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Reviewed, UniProtKB/Swiss-Prot Q93WF1 (PEL20_ARATH)

Last modified February 9, 2010. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Probable pectate lyase 20
    EC=4.2.2.2
Gene names
Ordered Locus Names: At5g48900
ORF Names: K19E20.1
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactor

Binds 1 calcium ion. Required for its activity By similarity.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.

Sequence similarities

Belongs to the polysaccharide lyase 1 family.

Sequence caution

The sequence BAB10313.1 differs from that shown. Reason: Erroneous gene model prediction.

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Ontologies

Keywords
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionLyase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

pectate lyase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 417392Probable pectate lyase 20
PRO_0000024885

Sites

Active site2951 Potential
Metal binding2151Calcium By similarity
Metal binding2391Calcium By similarity
Metal binding2431Calcium By similarity

Amino acid modifications

Glycosylation291N-linked (GlcNAc...) Potential
Glycosylation531N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
Q93WF1-1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 286C71B9AE957848

FASTA41746,728
        10         20         30         40         50         60 
MAVTQILVVF ASALLLSMFF TGVDSTRSNE TWHEHAVENP EEVAAMVDMS IRNSTARRRL 

        70         80         90        100        110        120 
GYFSCSTGNP IDDCWRCDRR WQSRRKHLAN CAIGFGRNAI GGRDGRYYVV SDPNDDNPVN 

       130        140        150        160        170        180 
PKPGTLRHAV IQEEPLWIVF KRDMVITLKE ELIMNSFKTI DGRGVNVHIA NGACITIQFV 

       190        200        210        220        230        240 
TNIIIHGIHI HDCRPTGNAM VRSSPSHYGW RTMADGDGIS IFGSSHIWID HNSLSNCADG 

       250        260        270        280        290        300 
LIDAVMASTA ITISNNYFTH HNEVMLLGHS DTYTRDKVMQ VTIAYNHFGE GLIQRMPRCR 

       310        320        330        340        350        360 
HGYFHVVNND YTHWEMYAIG GSASPTINSQ GNRYLAPRNR FAKEVTKRDY AGQWQWRHWN 

       370        380        390        400        410 
WRSEGDLFLN GAFFTRSGSG LGASYARASS LAAKSSSLVG VITYNAGALN CRGGRRC

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References

[1]"Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
DNA Res. 6:183-195(1999) [PubMed: 10470850] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[3]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB017061 Genomic DNA. Translation: BAB10313.1. Sequence problems.
AY050795 mRNA. Translation: AAK92730.1.
AY050404 mRNA. Translation: AAK91420.1.
AY086099 mRNA. Translation: AAM63307.1.
IPIIPI00520930.
RefSeqNP_568705.1.
UniGeneAt.26564

3D structure databases

SMRQ93WF1. Positions 68-410.
ModBaseSearch...

Protein family/group databases

CAZyPL1. Polysaccharide Lyase Family 1.

Proteomic databases

PRIDEQ93WF1.

Genome annotation databases

GeneID834948.
GenomeReviewsGene locus AT5G48900 in contig BA000015_GR.
KEGGath:AT5G48900.
NMPDRfig|3702.1.peg.26726.

Organism-specific databases

TAIRAt5g48900.

Phylogenomic databases

HOGENOMHBG317381.
InParanoidQ93WF1.
OMAHLANCAI.

Enzyme and pathway databases

BRENDA4.2.2.2. 302.

Gene expression databases

ArrayExpressQ93WF1.
GenevestigatorQ93WF1.
GermOnlineAT5G48900. Arabidopsis thaliana.

Family and domain databases

InterProIPR002022. Amb_allergen.
IPR018082. AmbAllergen.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
PfamPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSPR00807. AMBALLERGEN.
SMARTSM00656. Amb_all. 1 hit.
SM00710. PbH1. 2 hits.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePEL20_ARATH
AccessionPrimary (citable) accession number: Q93WF1
Secondary accession number(s): Q8LDB4, Q9FI81
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: December 1, 2001
Last modified: February 9, 2010
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents