Q93W54 (ICMTB_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase B
Short name=AtICMTB
EC=2.1.1.100

Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase B
Prenylated protein carboxyl methyltransferase B
Prenylcysteine carboxyl methyltransferase B

Gene names

Name:	ICMTB
Synonyms:	STE14B
Ordered Locus Names:	At5g08335
ORF Names:	F8L15.70

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues, resulting in the modulation of the function of prenylated proteins. Involved in negative regulation of abscisic acid signaling. Carboxyl methylation is a reversible and potentially regulated step in the post-translational modification of prenylated proteins. Ref.1 Ref.6
Catalytic activity	S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.
Cofactor	Divalent cations. Probably zinc By similarity.
Enzyme regulation	Inhibited by farnesylthioacetic acid (FTAA) and N-acetyl-S-trans, trans-farnesyl-l-cysteine (AFC).
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.5.
Tissue specificity	Expressed in flowers, stems, leaves, roots and siliques. Detected in apices and vascular tissues of leaves and roots, in the stigma and in the filaments and anthers of stamen. Not found in petioles or hypocotyls. Ref.1
Induction	Not induced by abscisic acid or auxin. Ref.6
Disruption phenotype	Plants lacking ICMTA and ICMTB have altered phyllotaxis, fasciated stems and development of axillary flowers. Ref.5
Miscellaneous	ICMTB is more widely expressed and has a higher catalytic activity than ICMTA.
Sequence similarities	Belongs to the isoprenylcysteine O-methyltransferase family.
Biophysicochemical properties	Kinetic parameters: KM=5.0 µM for AFC as methyl acceptor Ref.1 KM=3.0 µM for AGGC as methyl acceptor Vmax=220 pmol/min/mg enzyme toward AFC as methyl acceptor Vmax=296 pmol/min/mg enzyme toward AGGC as methyl acceptor
Sequence caution	The sequence CAC08334.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	flower development Inferred from mutant phenotype Ref.5. Source: TAIR negative regulation of abscisic acid mediated signaling pathway Non-traceable author statement Ref.1. Source: TAIR shoot development Inferred from mutant phenotype Ref.5. Source: TAIR
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.5. Source: TAIR endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Inferred from direct assay Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	Protein-S-isoprenylcysteine O-methyltransferase B		PRO_0000356250
Regions
	Transmembrane	16 – 36	21	Helical; Potential
	Transmembrane	52 – 72	21	Helical; Potential
	Transmembrane	81 – 101	21	Helical; Potential
	Transmembrane	140 – 160	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q93W54 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 54EBEBEBADBF97DB	FASTA	197	22,766
	10 20 30 40 50 60 MTEIFSDTGF RQLTQMFLAI IFFHTSEYIL AIAIHGASKV TLSSLLISKH YALAMLISVL 70 80 90 100 110 120 EYIAEIVFFP GLKQHWWISN FGLTMIILGE ILRKTAIITA GRSFTHLIKI RREEHHKLVT 130 140 150 160 170 180 EGVYQIMRHP SYSGFLIWSV GTQVMLCNPI SAIAFAVVVW RFFAERIPYE EHYLKQFFGR 190 QYVEYAQRVP SGVPFVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prenylcysteine alpha-carboxyl methyltransferase expression and function in Arabidopsis thaliana." Narasimha Chary S., Bultema R.L., Packard C.E., Crowell D.N. Plant J. 32:735-747(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Functional analysis of Arabidopsis postprenylation CaaX processing enzymes and their function in subcellular protein targeting." Bracha-Drori K., Shichrur K., Lubetzky T.C., Yalovsky S. Plant Physiol. 148:119-131(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[6]	"Isoprenylcysteine methylation and demethylation regulate abscisic acid signaling in Arabidopsis." Huizinga D.H., Omosegbon O., Omery B., Crowell D.N. Plant Cell 20:2714-2728(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL392174 Genomic DNA. Translation: CAC08334.1. Sequence problems. CP002688 Genomic DNA. Translation: AED91285.1. AY035028 mRNA. Translation: AAK59533.1. AY059102 mRNA. Translation: AAL15208.1.
IPI	IPI00530857.
RefSeq	NP_568191.1. NM_120917.1.
UniGene	At.19166.
3D structure databases
ProteinModelPortal	Q93W54.
SMR	Q93W54. Positions 101-185.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q93W54. 2 interactions.
STRING	3702.AT5G08335.1-P.
Proteomic databases
PaxDb	Q93W54.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G08335.1; AT5G08335.1; AT5G08335.
GeneID	830731.
KEGG	ath:AT5G08335.
Organism-specific databases
TAIR	At5g08335.
Phylogenomic databases
eggNOG	COG2020.
HOGENOM	HOG000213961.
InParanoid	Q93W54.
KO	K00587.
OMA	MLISVLE.
PhylomeDB	Q93W54.
ProtClustDB	CLSN2686135.
Enzyme and pathway databases
BioCyc	MetaCyc:AT5G08335-MONOMER.
SABIO-RK	Q93W54.
Gene expression databases
Genevestigator	Q93W54.
Family and domain databases
InterPro	IPR007269. ICMT_MeTrfase. IPR025770. PPMT_MeTrfase. [Graphical view]
Pfam	PF04140. ICMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ICMTB_ARATH

Accession

Primary (citable) accession number: Q93W54
Secondary accession number(s): Q9FTA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program