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Q93W54

- ICMTB_ARATH

UniProt

Q93W54 - ICMTB_ARATH

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Protein

Protein-S-isoprenylcysteine O-methyltransferase B

Gene

ICMTB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues, resulting in the modulation of the function of prenylated proteins. Involved in negative regulation of abscisic acid signaling. Carboxyl methylation is a reversible and potentially regulated step in the post-translational modification of prenylated proteins.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.

Cofactori

Divalent cations. Probably zinc By similarity.By similarity

Enzyme regulationi

Inhibited by farnesylthioacetic acid (FTAA) and N-acetyl-S-trans, trans-farnesyl-l-cysteine (AFC).

Kineticsi

  1. KM=5.0 µM for AFC as methyl acceptor1 Publication
  2. KM=3.0 µM for AGGC as methyl acceptor1 Publication

Vmax=220 pmol/min/mg enzyme toward AFC as methyl acceptor1 Publication

Vmax=296 pmol/min/mg enzyme toward AGGC as methyl acceptor1 Publication

GO - Molecular functioni

  1. protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Source: TAIR

GO - Biological processi

  1. flower development Source: TAIR
  2. negative regulation of abscisic acid-activated signaling pathway Source: TAIR
  3. shoot system development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT5G08335-MONOMER.
MetaCyc:AT5G08335-MONOMER.
SABIO-RKQ93W54.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase B (EC:2.1.1.100)
Short name:
AtICMTB
Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase B
Prenylated protein carboxyl methyltransferase B
Prenylcysteine carboxyl methyltransferase B
Gene namesi
Name:ICMTB
Synonyms:STE14B
Ordered Locus Names:At5g08335
ORF Names:F8L15.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G08335.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: TAIR
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Plants lacking ICMTA and ICMTB have altered phyllotaxis, fasciated stems and development of axillary flowers.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 197197Protein-S-isoprenylcysteine O-methyltransferase BPRO_0000356250Add
BLAST

Proteomic databases

PaxDbiQ93W54.
PRIDEiQ93W54.

Expressioni

Tissue specificityi

Expressed in flowers, stems, leaves, roots and siliques. Detected in apices and vascular tissues of leaves and roots, in the stigma and in the filaments and anthers of stamen. Not found in petioles or hypocotyls.1 Publication

Inductioni

Not induced by abscisic acid or auxin.1 Publication

Gene expression databases

GenevestigatoriQ93W54.

Interactioni

Protein-protein interaction databases

BioGridi16009. 2 interactions.
IntActiQ93W54. 2 interactions.
STRINGi3702.AT5G08335.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ93W54.
SMRiQ93W54. Positions 101-185.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3621HelicalSequence AnalysisAdd
BLAST
Transmembranei52 – 7221HelicalSequence AnalysisAdd
BLAST
Transmembranei81 – 10121HelicalSequence AnalysisAdd
BLAST
Transmembranei140 – 16021HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2020.
HOGENOMiHOG000213961.
InParanoidiQ93W54.
KOiK00587.
OMAiMPNDAST.
PhylomeDBiQ93W54.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q93W54-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEIFSDTGF RQLTQMFLAI IFFHTSEYIL AIAIHGASKV TLSSLLISKH
60 70 80 90 100
YALAMLISVL EYIAEIVFFP GLKQHWWISN FGLTMIILGE ILRKTAIITA
110 120 130 140 150
GRSFTHLIKI RREEHHKLVT EGVYQIMRHP SYSGFLIWSV GTQVMLCNPI
160 170 180 190
SAIAFAVVVW RFFAERIPYE EHYLKQFFGR QYVEYAQRVP SGVPFVN
Length:197
Mass (Da):22,766
Last modified:December 1, 2001 - v1
Checksum:i54EBEBEBADBF97DB
GO

Sequence cautioni

The sequence CAC08334.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL392174 Genomic DNA. Translation: CAC08334.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED91285.1.
AY035028 mRNA. Translation: AAK59533.1.
AY059102 mRNA. Translation: AAL15208.1.
RefSeqiNP_568191.1. NM_120917.1.
UniGeneiAt.19166.

Genome annotation databases

EnsemblPlantsiAT5G08335.1; AT5G08335.1; AT5G08335.
GeneIDi830731.
KEGGiath:AT5G08335.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL392174 Genomic DNA. Translation: CAC08334.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED91285.1 .
AY035028 mRNA. Translation: AAK59533.1 .
AY059102 mRNA. Translation: AAL15208.1 .
RefSeqi NP_568191.1. NM_120917.1.
UniGenei At.19166.

3D structure databases

ProteinModelPortali Q93W54.
SMRi Q93W54. Positions 101-185.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 16009. 2 interactions.
IntActi Q93W54. 2 interactions.
STRINGi 3702.AT5G08335.1-P.

Proteomic databases

PaxDbi Q93W54.
PRIDEi Q93W54.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G08335.1 ; AT5G08335.1 ; AT5G08335 .
GeneIDi 830731.
KEGGi ath:AT5G08335.

Organism-specific databases

TAIRi AT5G08335.

Phylogenomic databases

eggNOGi COG2020.
HOGENOMi HOG000213961.
InParanoidi Q93W54.
KOi K00587.
OMAi MPNDAST.
PhylomeDBi Q93W54.

Enzyme and pathway databases

BioCyci ARA:AT5G08335-MONOMER.
MetaCyc:AT5G08335-MONOMER.
SABIO-RK Q93W54.

Gene expression databases

Genevestigatori Q93W54.

Family and domain databases

InterProi IPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view ]
Pfami PF04140. ICMT. 1 hit.
[Graphical view ]
PROSITEi PS51564. SAM_ICMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prenylcysteine alpha-carboxyl methyltransferase expression and function in Arabidopsis thaliana."
    Narasimha Chary S., Bultema R.L., Packard C.E., Crowell D.N.
    Plant J. 32:735-747(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Functional analysis of Arabidopsis postprenylation CaaX processing enzymes and their function in subcellular protein targeting."
    Bracha-Drori K., Shichrur K., Lubetzky T.C., Yalovsky S.
    Plant Physiol. 148:119-131(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  6. "Isoprenylcysteine methylation and demethylation regulate abscisic acid signaling in Arabidopsis."
    Huizinga D.H., Omosegbon O., Omery B., Crowell D.N.
    Plant Cell 20:2714-2728(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiICMTB_ARATH
AccessioniPrimary (citable) accession number: Q93W54
Secondary accession number(s): Q9FTA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

ICMTB is more widely expressed and has a higher catalytic activity than ICMTA.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3