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Q93W54

- ICMTB_ARATH

UniProt

Q93W54 - ICMTB_ARATH

Protein

Protein-S-isoprenylcysteine O-methyltransferase B

Gene

ICMTB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues, resulting in the modulation of the function of prenylated proteins. Involved in negative regulation of abscisic acid signaling. Carboxyl methylation is a reversible and potentially regulated step in the post-translational modification of prenylated proteins.2 Publications

    Catalytic activityi

    S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.

    Cofactori

    Divalent cations. Probably zinc By similarity.By similarity

    Enzyme regulationi

    Inhibited by farnesylthioacetic acid (FTAA) and N-acetyl-S-trans, trans-farnesyl-l-cysteine (AFC).

    Kineticsi

    1. KM=5.0 µM for AFC as methyl acceptor1 Publication
    2. KM=3.0 µM for AGGC as methyl acceptor1 Publication

    Vmax=220 pmol/min/mg enzyme toward AFC as methyl acceptor1 Publication

    Vmax=296 pmol/min/mg enzyme toward AGGC as methyl acceptor1 Publication

    GO - Molecular functioni

    1. protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Source: TAIR

    GO - Biological processi

    1. flower development Source: TAIR
    2. negative regulation of abscisic acid-activated signaling pathway Source: TAIR
    3. shoot system development Source: TAIR

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciARA:AT5G08335-MONOMER.
    MetaCyc:AT5G08335-MONOMER.
    SABIO-RKQ93W54.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein-S-isoprenylcysteine O-methyltransferase B (EC:2.1.1.100)
    Short name:
    AtICMTB
    Alternative name(s):
    Isoprenylcysteine carboxylmethyltransferase B
    Prenylated protein carboxyl methyltransferase B
    Prenylcysteine carboxyl methyltransferase B
    Gene namesi
    Name:ICMTB
    Synonyms:STE14B
    Ordered Locus Names:At5g08335
    ORF Names:F8L15.70
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G08335.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: TAIR
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Plants lacking ICMTA and ICMTB have altered phyllotaxis, fasciated stems and development of axillary flowers.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 197197Protein-S-isoprenylcysteine O-methyltransferase BPRO_0000356250Add
    BLAST

    Proteomic databases

    PaxDbiQ93W54.
    PRIDEiQ93W54.

    Expressioni

    Tissue specificityi

    Expressed in flowers, stems, leaves, roots and siliques. Detected in apices and vascular tissues of leaves and roots, in the stigma and in the filaments and anthers of stamen. Not found in petioles or hypocotyls.1 Publication

    Inductioni

    Not induced by abscisic acid or auxin.1 Publication

    Gene expression databases

    GenevestigatoriQ93W54.

    Interactioni

    Protein-protein interaction databases

    BioGridi16009. 2 interactions.
    IntActiQ93W54. 2 interactions.
    STRINGi3702.AT5G08335.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ93W54.
    SMRiQ93W54. Positions 101-185.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei52 – 7221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei81 – 10121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei140 – 16021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2020.
    HOGENOMiHOG000213961.
    InParanoidiQ93W54.
    KOiK00587.
    OMAiMPNDAST.
    PhylomeDBiQ93W54.

    Family and domain databases

    InterProiIPR007269. ICMT_MeTrfase.
    IPR025770. PPMT_MeTrfase.
    [Graphical view]
    PfamiPF04140. ICMT. 1 hit.
    [Graphical view]
    PROSITEiPS51564. SAM_ICMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q93W54-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEIFSDTGF RQLTQMFLAI IFFHTSEYIL AIAIHGASKV TLSSLLISKH    50
    YALAMLISVL EYIAEIVFFP GLKQHWWISN FGLTMIILGE ILRKTAIITA 100
    GRSFTHLIKI RREEHHKLVT EGVYQIMRHP SYSGFLIWSV GTQVMLCNPI 150
    SAIAFAVVVW RFFAERIPYE EHYLKQFFGR QYVEYAQRVP SGVPFVN 197
    Length:197
    Mass (Da):22,766
    Last modified:December 1, 2001 - v1
    Checksum:i54EBEBEBADBF97DB
    GO

    Sequence cautioni

    The sequence CAC08334.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL392174 Genomic DNA. Translation: CAC08334.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED91285.1.
    AY035028 mRNA. Translation: AAK59533.1.
    AY059102 mRNA. Translation: AAL15208.1.
    RefSeqiNP_568191.1. NM_120917.1.
    UniGeneiAt.19166.

    Genome annotation databases

    EnsemblPlantsiAT5G08335.1; AT5G08335.1; AT5G08335.
    GeneIDi830731.
    KEGGiath:AT5G08335.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL392174 Genomic DNA. Translation: CAC08334.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED91285.1 .
    AY035028 mRNA. Translation: AAK59533.1 .
    AY059102 mRNA. Translation: AAL15208.1 .
    RefSeqi NP_568191.1. NM_120917.1.
    UniGenei At.19166.

    3D structure databases

    ProteinModelPortali Q93W54.
    SMRi Q93W54. Positions 101-185.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 16009. 2 interactions.
    IntActi Q93W54. 2 interactions.
    STRINGi 3702.AT5G08335.1-P.

    Proteomic databases

    PaxDbi Q93W54.
    PRIDEi Q93W54.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G08335.1 ; AT5G08335.1 ; AT5G08335 .
    GeneIDi 830731.
    KEGGi ath:AT5G08335.

    Organism-specific databases

    TAIRi AT5G08335.

    Phylogenomic databases

    eggNOGi COG2020.
    HOGENOMi HOG000213961.
    InParanoidi Q93W54.
    KOi K00587.
    OMAi MPNDAST.
    PhylomeDBi Q93W54.

    Enzyme and pathway databases

    BioCyci ARA:AT5G08335-MONOMER.
    MetaCyc:AT5G08335-MONOMER.
    SABIO-RK Q93W54.

    Gene expression databases

    Genevestigatori Q93W54.

    Family and domain databases

    InterProi IPR007269. ICMT_MeTrfase.
    IPR025770. PPMT_MeTrfase.
    [Graphical view ]
    Pfami PF04140. ICMT. 1 hit.
    [Graphical view ]
    PROSITEi PS51564. SAM_ICMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prenylcysteine alpha-carboxyl methyltransferase expression and function in Arabidopsis thaliana."
      Narasimha Chary S., Bultema R.L., Packard C.E., Crowell D.N.
      Plant J. 32:735-747(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Functional analysis of Arabidopsis postprenylation CaaX processing enzymes and their function in subcellular protein targeting."
      Bracha-Drori K., Shichrur K., Lubetzky T.C., Yalovsky S.
      Plant Physiol. 148:119-131(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    6. "Isoprenylcysteine methylation and demethylation regulate abscisic acid signaling in Arabidopsis."
      Huizinga D.H., Omosegbon O., Omery B., Crowell D.N.
      Plant Cell 20:2714-2728(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.

    Entry informationi

    Entry nameiICMTB_ARATH
    AccessioniPrimary (citable) accession number: Q93W54
    Secondary accession number(s): Q9FTA1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 16, 2008
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    ICMTB is more widely expressed and has a higher catalytic activity than ICMTA.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3