ID NIFU2_ARATH Reviewed; 235 AA. AC Q93W20; A8MS35; Q9LTX6; DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot. DT 01-DEC-2001, sequence version 1. DT 25-JAN-2012, entry version 59. DE RecName: Full=NifU-like protein 2, chloroplastic; DE Short=AtCNfu2; DE Short=AtCnfU-V; DE Flags: Precursor; GN Name=NIFU2; Synonyms=CNFU2, NFU2; OrderedLocusNames=At5g49940; GN ORFNames=K9P8.16; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, RP SUBCELLULAR LOCATION, AND GENE FAMILY. RC STRAIN=cv. Columbia; RX PubMed=12553879; DOI=10.1042/BJ20021946; RA Leon S., Touraine B., Ribot C., Briat J.-F., Lobreaux S.; RT "Iron-sulphur cluster assembly in plants: distinct NFU proteins in RT mitochondria and plastids from Arabidopsis thaliana."; RL Biochem. J. 371:823-830(2003). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX MEDLINE=20181125; PubMed=10718197; DOI=10.1093/dnares/7.1.31; RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., RA Tabata S.; RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC RT clones."; RL DNA Res. 7:31-63(2000). RN [3] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RG The Arabidopsis Information Resource (TAIR); RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RC STRAIN=cv. Columbia; RX MEDLINE=21932900; PubMed=11910074; DOI=10.1126/science.1071006; RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., RA Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., RA Shibata K., Shinagawa A., Shinozaki K.; RT "Functional annotation of a full-length Arabidopsis cDNA collection."; RL Science 296:141-145(2002). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RC STRAIN=cv. Columbia; RX MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RC STRAIN=cv. Columbia; RX PubMed=19423640; DOI=10.1093/dnares/dsp009; RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., RA Seki M., Shinozaki K.; RT "Analysis of multiple occurrences of alternative splicing events in RT Arabidopsis thaliana using novel sequenced full-length cDNAs."; RL DNA Res. 16:155-164(2009). RN [7] RP FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, RP AND DISRUPTION PHENOTYPE. RX PubMed=15031412; DOI=10.1105/tpc.020511; RA Yabe T., Morimoto K., Kikuchi S., Nishio K., Terashima I., Nakai M.; RT "The Arabidopsis chloroplastic NifU-like protein CnfU, which can act RT as an iron-sulfur cluster scaffold protein, is required for biogenesis RT of ferredoxin and photosystem I."; RL Plant Cell 16:993-1007(2004). RN [8] RP FUNCTION. RX PubMed=15361144; DOI=10.1111/j.1365-313X.2004.02189.x; RA Touraine B., Boutin J.-P., Marion-Poll A., Briat J.-F., Peltier G., RA Lobreaux S.; RT "Nfu2: a scaffold protein required for [4Fe-4S] and ferredoxin iron- RT sulphur cluster assembly in Arabidopsis chloroplasts."; RL Plant J. 40:101-111(2004). RN [9] RP X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 83-235, SUBUNIT, AND RP DISULFIDE BONDS. RX PubMed=18585737; DOI=10.1016/j.jmb.2008.05.072; RA Yabe T., Yamashita E., Kikuchi A., Morimoto K., Nakagawa A., RA Tsukihara T., Nakai M.; RT "Structural analysis of Arabidopsis CnfU protein: an iron-sulfur RT cluster biosynthetic scaffold in chloroplasts."; RL J. Mol. Biol. 381:160-173(2008). CC -!- FUNCTION: Molecular scaffold for [Fe-S] cluster assembly of CC chloroplastic iron-sulfur proteins. Required for biogenesis of CC ferredoxin, a major photosynthetic electron carrier containing CC [2Fe-2S] cluster. Required for the assembly of photosystem I CC complex. CC -!- COFACTOR: Binds 1 [2Fe-2S] cluster per subunit. CC -!- SUBUNIT: Homodimer; disulfide-linked. CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1; CC IsoId=Q93W20-1; Sequence=Displayed; CC Name=2; CC IsoId=Q93W20-2; Sequence=VSP_040522, VSP_040523; CC Note=May be due to a competing donor splice site. No CC experimental confirmation available; CC -!- TISSUE SPECIFICITY: Predominantly expressed in leaves and floral CC stalks. Ubiquitous (at protein level). CC -!- DISRUPTION PHENOTYPE: Plants are dwarf with faint pale-green CC leaves, decreased amount of ferredoxin and impaired photosystem I CC accumulation. CC -!- SIMILARITY: Belongs to the nifU family. CC -!- SEQUENCE CAUTION: CC Sequence=BAA97015.1; Type=Erroneous gene model prediction; Note=The predicted gene At5g49940 has been split into 2 genes: At5g49940 and At5g49945; CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; AJ512934; CAD55559.1; -; mRNA. DR EMBL; AB024032; BAA97015.1; ALT_SEQ; Genomic_DNA. DR EMBL; CP002688; AED95873.1; -; Genomic_DNA. DR EMBL; CP002688; AED95874.1; -; Genomic_DNA. DR EMBL; AK316745; BAH19468.1; -; mRNA. DR EMBL; AF370353; AAK44168.1; -; mRNA. DR EMBL; AF428399; AAL16167.1; -; mRNA. DR EMBL; AY062984; AAL34158.1; -; mRNA. DR EMBL; AK118652; BAC43248.1; -; mRNA. DR IPI; IPI00517598; -. DR IPI; IPI00846628; -. DR RefSeq; NP_001078739.1; NM_001085270.2. DR RefSeq; NP_568715.1; NM_124372.4. DR UniGene; At.26219; -. DR PDB; 2Z51; X-ray; 1.35 A; A=83-235. DR PDBsum; 2Z51; -. DR ProteinModelPortal; Q93W20; -. DR SMR; Q93W20; 83-235. DR STRING; Q93W20; -. DR PRIDE; Q93W20; -. DR EnsemblPlants; AT5G49940.1; AT5G49940.1; AT5G49940. DR GeneID; 835057; -. DR GenomeReviews; BA000015_GR; AT5G49940. DR KEGG; ath:AT5G49940; -. DR NMPDR; fig|3702.1.peg.26841; -. DR GeneFarm; 3943; 402. DR TAIR; At5g49940; -. DR GeneTree; EPGT00070000029156; -. DR HOGENOM; HBG749723; -. DR InParanoid; Q93W20; -. DR OMA; VRPYLMA; -. DR PhylomeDB; Q93W20; -. DR ProtClustDB; CLSN2720187; -. DR ArrayExpress; Q93W20; -. DR Genevestigator; Q93W20; -. DR GermOnline; AT5G49940; Arabidopsis thaliana. DR GO; GO:0009570; C:chloroplast stroma; IDA:TAIR. DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW. DR GO; GO:0005506; F:iron ion binding; IEA:InterPro. DR GO; GO:0005198; F:structural molecule activity; TAS:TAIR. DR GO; GO:0009658; P:chloroplast organization; IMP:TAIR. DR GO; GO:0016226; P:iron-sulfur cluster assembly; IMP:TAIR. DR InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C. DR Pfam; PF01106; NifU; 2. DR ProDom; PD002830; NIF_FeS_clus_asmbl_NifU_C; 2. PE 1: Evidence at protein level; KW 2Fe-2S; 3D-structure; Alternative splicing; Chloroplast; KW Complete proteome; Disulfide bond; Iron; Iron-sulfur; Metal-binding; KW Plastid; Reference proteome; Transit peptide. FT TRANSIT 1 16 Chloroplast (Potential). FT CHAIN 17 235 NifU-like protein 2, chloroplastic. FT /FTId=PRO_0000247613. FT DISULFID 126 126 Interchain (with C-129). FT DISULFID 129 129 Interchain (with C-126). FT VAR_SEQ 175 185 LEEIRPYLIGT -> KFWCWKKSGLT (in isoform FT 2). FT /FTId=VSP_040522. FT VAR_SEQ 186 235 Missing (in isoform 2). FT /FTId=VSP_040523. FT HELIX 87 103 FT STRAND 108 113 FT STRAND 117 122 FT HELIX 130 132 FT HELIX 133 137 FT HELIX 141 147 FT STRAND 153 156 FT HELIX 168 179 FT STRAND 190 196 FT STRAND 199 204 FT HELIX 213 225 FT STRAND 231 234 SQ SEQUENCE 235 AA; 25620 MW; 6443A3A407D8A3A8 CRC64; MQLLTLNPAA ISRTPPQAID PSSSSSLLLP FPQILSSQRA LGLVARPCNP LRRGLSRFLS SRQLFRRSKV VKAVATPDPI LEVPLTEENV ESVLDEIRPY LMSDGGNVAL HEIDGNIVRV KLQGACGSCP SSTMTMKMGI ERRLMEKIPE IVAVEALPDE ETGLELNEEN IEKVLEEIRP YLIGTADGSL DLVEIEDPIV KIRITGPAAG VMTVRVAVTQ KLREKIPSIA AVQLI //