Q93W20 (NIFU2_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 69.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: NifU-like protein 2, chloroplastic Short name=AtCNfu2 Short name=AtCnfU-V | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Reference proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis![]() |
Protein attributes
| Sequence length | 235 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Molecular scaffold for [Fe-S] cluster assembly of chloroplastic iron-sulfur proteins. Required for biogenesis of ferredoxin, a major photosynthetic electron carrier containing [2Fe-2S] cluster. Required for the assembly of photosystem I complex. Ref.7 Ref.8 |
| Cofactor | Binds 1 [2Fe-2S] cluster per subunit. Ref.7 |
| Subunit structure | |
| Subcellular location | |
| Tissue specificity | Predominantly expressed in leaves and floral stalks. Ubiquitous (at protein level). Ref.1 Ref.7 |
| Disruption phenotype | Plants are dwarf with faint pale-green leaves, decreased amount of ferredoxin and impaired photosystem I accumulation. Ref.7 |
| Sequence similarities | Belongs to the NifU family. |
| Sequence caution | The sequence BAA97015.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At5g49940 has been split into 2 genes: At5g49940 and At5g49945. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Chloroplast Plastid |
| Coding sequence diversity | Alternative splicing |
| Domain | Transit peptide |
| Ligand | 2Fe-2S Iron Iron-sulfur Metal-binding |
| PTM | Disulfide bond |
| Technical term | 3D-structure Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | chloroplast organization Inferred from mutant phenotype Ref.7. Source: TAIR iron-sulfur cluster assemblyInferred from mutant phenotype Ref.7. Source: TAIR |
| Cellular_component | chloroplast stroma Inferred from direct assay PubMed 20061580. Source: TAIR |
| Molecular_function | 2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion bindingInferred from electronic annotation. Source: InterPro structural molecule activityTraceable author statement Ref.1. Source: TAIR |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q93W20-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q93W20-2) The sequence of this isoform differs from the canonical sequence as follows: 175-185: LEEIRPYLIGT → KFWCWKKSGLT 186-235: Missing. | ||||||
| Note: May be due to a competing donor splice site. No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 16 | 16 | Chloroplast Potential | ||||||||||||||||||||||||||||||||
| Chain | 17 – 235 | 219 | NifU-like protein 2, chloroplastic | PRO_0000247613 | |||||||||||||||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||||||||||||||||
| Disulfide bond | 126 | Interchain (with C-129) Ref.9 | |||||||||||||||||||||||||||||||||
| Disulfide bond | 129 | Interchain (with C-126) Ref.9 | |||||||||||||||||||||||||||||||||
Natural variations | |||||||||||||||||||||||||||||||||||
| Alternative sequence | 175 – 185 | 11 | LEEIRPYLIGT → KFWCWKKSGLT in isoform 2. | VSP_040522 | |||||||||||||||||||||||||||||||
| Alternative sequence | 186 – 235 | 50 | Missing in isoform 2. | VSP_040523 | |||||||||||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||||||||||||
| Helix | 87 – 103 | 17 | |||||||||||||||||||||||||||||||||
| Beta strand | 106 – 114 | 9 | |||||||||||||||||||||||||||||||||
| Beta strand | 117 – 123 | 7 | |||||||||||||||||||||||||||||||||
| Helix | 124 – 127 | 4 | |||||||||||||||||||||||||||||||||
| Helix | 130 – 132 | 3 | |||||||||||||||||||||||||||||||||
| Helix | 133 – 147 | 15 | |||||||||||||||||||||||||||||||||
| Beta strand | 153 – 156 | 4 | |||||||||||||||||||||||||||||||||
| Helix | 168 – 178 | 11 | |||||||||||||||||||||||||||||||||
| Helix | 179 – 181 | 3 | |||||||||||||||||||||||||||||||||
| Helix | 184 – 186 | 3 | |||||||||||||||||||||||||||||||||
| Beta strand | 189 – 196 | 8 | |||||||||||||||||||||||||||||||||
| Beta strand | 199 – 206 | 8 | |||||||||||||||||||||||||||||||||
| Helix | 207 – 210 | 4 | |||||||||||||||||||||||||||||||||
| Helix | 213 – 225 | 13 | |||||||||||||||||||||||||||||||||
| Beta strand | 231 – 234 | 4 | |||||||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Iron-sulphur cluster assembly in plants: distinct NFU proteins in mitochondria and plastids from Arabidopsis thaliana." Leon S., Touraine B., Ribot C., Briat J.-F., Lobreaux S. Biochem. J. 371:823-830(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY. Strain: cv. Columbia. |
| [2] | "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [4] | "Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia. |
| [5] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia. |
| [6] | "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia. |
| [7] | "The Arabidopsis chloroplastic NifU-like protein CnfU, which can act as an iron-sulfur cluster scaffold protein, is required for biogenesis of ferredoxin and photosystem I." Yabe T., Morimoto K., Kikuchi S., Nishio K., Terashima I., Nakai M. Plant Cell 16:993-1007(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE. |
| [8] | "Nfu2: a scaffold protein required for [4Fe-4S] and ferredoxin iron-sulphur cluster assembly in Arabidopsis chloroplasts." Touraine B., Boutin J.-P., Marion-Poll A., Briat J.-F., Peltier G., Lobreaux S. Plant J. 40:101-111(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. |
| [9] | "Structural analysis of Arabidopsis CnfU protein: an iron-sulfur cluster biosynthetic scaffold in chloroplasts." Yabe T., Yamashita E., Kikuchi A., Morimoto K., Nakagawa A., Tsukihara T., Nakai M. J. Mol. Biol. 381:160-173(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 83-235, SUBUNIT, DISULFIDE BONDS. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | AJ512934 mRNA. Translation: CAD55559.1. AB024032 Genomic DNA. Translation: BAA97015.1. Sequence problems. CP002688 Genomic DNA. Translation: AED95873.1. CP002688 Genomic DNA. Translation: AED95874.1. AK316745 mRNA. Translation: BAH19468.1. AF370353 mRNA. Translation: AAK44168.1. AF428399 mRNA. Translation: AAL16167.1. AY062984 mRNA. Translation: AAL34158.1. AK118652 mRNA. Translation: BAC43248.1. | ||||||||||||
| IPI | IPI00517598. IPI00846628. | ||||||||||||
| RefSeq | NP_001078739.1. NM_001085270.2. NP_568715.1. NM_124372.4. | ||||||||||||
| UniGene | At.26219. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | Q93W20. | ||||||||||||
| SMR | Q93W20. Positions 83-235. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| STRING | 3702.AT5G49940.1-P. | ||||||||||||
Proteomic databases | |||||||||||||
| PaxDb | Q93W20. | ||||||||||||
| PRIDE | Q93W20. | ||||||||||||
Protocols and materials databases | |||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| EnsemblPlants | AT5G49940.1; AT5G49940.1; AT5G49940. | ||||||||||||
| GeneID | 835057. | ||||||||||||
| KEGG | ath:AT5G49940. | ||||||||||||
Organism-specific databases | |||||||||||||
| GeneFarm | 3943. 402. | ||||||||||||
| TAIR | At5g49940. | ||||||||||||
Phylogenomic databases | |||||||||||||
| eggNOG | COG0694. | ||||||||||||
| HOGENOM | HOG000006174. | ||||||||||||
| InParanoid | Q93W20. | ||||||||||||
| OMA | LEFVTIE. | ||||||||||||
| PhylomeDB | Q93W20. | ||||||||||||
| ProtClustDB | CLSN2720187. | ||||||||||||
Gene expression databases | |||||||||||||
| Genevestigator | Q93W20. | ||||||||||||
| GermOnline | AT5G49940. Arabidopsis thaliana. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR001075. NIF_FeS_clus_asmbl_NifU_C. [Graphical view] | ||||||||||||
| Pfam | PF01106. NifU. 2 hits. [Graphical view] | ||||||||||||
| ProDom | PD002830. NIF_FeS_clus_asmbl_NifU_C. 2 hits. [Graphical view] [Entries sharing at least one domain] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other | |||||||||||||
| EvolutionaryTrace | Q93W20. | ||||||||||||
Entry information
| Entry name | NIFU2_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q93W20 Secondary accession number(s): A8MS35, Q9LTX6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Recent format changes Overview of recent format changes |
| Recent format changes (XML) Overview of recent format changes in the XML format |
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with
