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Reviewed, UniProtKB/Swiss-Prot Q93W20 (NIFU2_ARATH)

Last modified June 16, 2009. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    NifU-like protein 2, chloroplastic
      Short name=AtCNfu2
      Short name=AtCnfU-V
Gene names
Name: NIFU2
Synonyms: CNFU2, NFU2
Ordered Locus Names: At5g49940
ORF Names: K9P8.16
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length235 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Molecular scaffold for [Fe-S] cluster assembly of chloroplastic iron-sulfur proteins. Required for biogenesis of ferredoxin, a major photosynthetic electron carrier containing [2Fe-2S] cluster. Required for the assembly of photosystem I complex. Ref.5 Ref.6

Cofactor

Binds 1 [2Fe-2S] cluster per subunit. Ref.5

Subunit structure

Homodimer. Ref.5

Subcellular location

Plastidchloroplast stroma. Ref.5 Ref.1

Tissue specificity

Predominantly expressed in leaves and floral stalks. Ubiquitous (at protein level). Ref.5 Ref.1

Disruption phenotype

Plants are dwarf with faint pale-green leaves, decreased amount of ferredoxin and impaired photosystem I accumulation. Ref.5

Sequence similarities

Belongs to the nifU family.

Sequence caution

The sequence BAA97015.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At5g49940 has been split into 2 genes: At5g49940 and At5g49945.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q93W20-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1616Chloroplast Potential
Chain17 – 235219NifU-like protein 2, chloroplastic
PRO_0000247613

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 6443A3A407D8A3A8

FASTA23525,620
        10         20         30         40         50         60 
MQLLTLNPAA ISRTPPQAID PSSSSSLLLP FPQILSSQRA LGLVARPCNP LRRGLSRFLS 

        70         80         90        100        110        120 
SRQLFRRSKV VKAVATPDPI LEVPLTEENV ESVLDEIRPY LMSDGGNVAL HEIDGNIVRV 

       130        140        150        160        170        180 
KLQGACGSCP SSTMTMKMGI ERRLMEKIPE IVAVEALPDE ETGLELNEEN IEKVLEEIRP 

       190        200        210        220        230 
YLIGTADGSL DLVEIEDPIV KIRITGPAAG VMTVRVAVTQ KLREKIPSIA AVQLI 

« Hide

References

« Hide 'large scale' references
[1]"Iron-sulphur cluster assembly in plants: distinct NFU proteins in mitochondria and plastids from Arabidopsis thaliana."
Leon S., Touraine B., Ribot C., Briat J.-F., Lobreaux S.
Biochem. J. 371:823-830(2003) [PubMed: 12553879] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed: 11910074] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"The Arabidopsis chloroplastic NifU-like protein CnfU, which can act as an iron-sulfur cluster scaffold protein, is required for biogenesis of ferredoxin and photosystem I."
Yabe T., Morimoto K., Kikuchi S., Nishio K., Terashima I., Nakai M.
Plant Cell 16:993-1007(2004) [PubMed: 15031412] [Abstract]
Cited for: FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[6]"Nfu2: a scaffold protein required for [4Fe-4S] and ferredoxin iron-sulphur cluster assembly in Arabidopsis chloroplasts."
Touraine B., Boutin J.-P., Marion-Poll A., Briat J.-F., Peltier G., Lobreaux S.
Plant J. 40:101-111(2004) [PubMed: 15361144] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

AJ512934 mRNA. Translation: CAD55559.1.
AB024032 Genomic DNA. Translation: BAA97015.1. Sequence problems.
AF370353 mRNA. Translation: AAK44168.1.
AF428399 mRNA. Translation: AAL16167.1.
AY062984 mRNA. Translation: AAL34158.1.
AK118652 mRNA. Translation: BAC43248.1.
IPIIPI00517598.
RefSeqNP_568715.1.
UniGeneAt.26219

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2Z51X-ray1.35A83-235[»]
ModBaseSearch...

Proteomic databases

PRIDEQ93W20.

Genome annotation databases

GeneID835057.
GenomeReviewsGene locus AT5G49940 in contig BA000015_GR.
NMPDRfig|3702.1.peg.26841.

Organism-specific databases

GeneFarm3943. 402.
TAIRAt5g49940.

Phylogenomic databases

OMAQ93W20. LHEIDGN.

Gene expression databases

ArrayExpressQ93W20.
GermOnlineAT5G49940. Arabidopsis thaliana.

Family and domain databases

InterProIPR001075. NIF_FeS_clus_asmbl_NifU_C.
[Graphical view]
PfamPF01106. NifU. 2 hits.
[Graphical view]
ProDomPD002830. NifU_C. 2 hits.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameNIFU2_ARATH
AccessionPrimary (citable) accession number: Q93W20
Secondary accession number(s): Q9LTX6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: December 1, 2001
Last modified: June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Recent format changes

Overview of recent format changes

Recent format changes (XML)

Overview of recent format changes in the XML format

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents