Reviewed, UniProtKB/Swiss-Prot Q93W20 (NIFU2_ARATH)

Names and origin

Protein names

Recommended name:
    NifU-like protein 2, chloroplastic
      Short name=AtCNfu2
      Short name=AtCnfU-V

Gene names

Name:	NIFU2
Synonyms:	CNFU2, NFU2
Ordered Locus Names:	At5g49940
ORF Names:	K9P8.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Molecular scaffold for [Fe-S] cluster assembly of chloroplastic iron-sulfur proteins. Required for biogenesis of ferredoxin, a major photosynthetic electron carrier containing [2Fe-2S] cluster. Required for the assembly of photosystem I complex. Ref.5 Ref.6
Cofactor	Binds 1 [2Fe-2S] cluster per subunit. Ref.5
Subunit structure	Homodimer. Ref.5
Subcellular location	Plastid › chloroplast stroma. Ref.5 Ref.1
Tissue specificity	Predominantly expressed in leaves and floral stalks. Ubiquitous (at protein level). Ref.5 Ref.1
Disruption phenotype	Plants are dwarf with faint pale-green leaves, decreased amount of ferredoxin and impaired photosystem I accumulation. Ref.5
Sequence similarities	Belongs to the nifU family.
Sequence caution	The sequence BAA97015.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At5g49940 has been split into 2 genes: At5g49940 and At5g49945.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	chloroplast organization Ref.5 Inferred from mutant phenotype. Source: TAIR iron-sulfur cluster assembly Ref.1 Ref.5 Inferred from mutant phenotype. Source: TAIR
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Ref.1 Traceable author statement. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q93W20-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 16	16	Chloroplast Potential
	Chain	17 – 235	219	NifU-like protein 2, chloroplastic		PRO_0000247613

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 6443A3A407D8A3A8	FASTA	235	25,620
	10 20 30 40 50 60 MQLLTLNPAA ISRTPPQAID PSSSSSLLLP FPQILSSQRA LGLVARPCNP LRRGLSRFLS 70 80 90 100 110 120 SRQLFRRSKV VKAVATPDPI LEVPLTEENV ESVLDEIRPY LMSDGGNVAL HEIDGNIVRV 130 140 150 160 170 180 KLQGACGSCP SSTMTMKMGI ERRLMEKIPE IVAVEALPDE ETGLELNEEN IEKVLEEIRP 190 200 210 220 230 YLIGTADGSL DLVEIEDPIV KIRITGPAAG VMTVRVAVTQ KLREKIPSIA AVQLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Iron-sulphur cluster assembly in plants: distinct NFU proteins in mitochondria and plastids from Arabidopsis thaliana." Leon S., Touraine B., Ribot C., Briat J.-F., Lobreaux S. Biochem. J. 371:823-830(2003) [PubMed: 12553879] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The Arabidopsis chloroplastic NifU-like protein CnfU, which can act as an iron-sulfur cluster scaffold protein, is required for biogenesis of ferredoxin and photosystem I." Yabe T., Morimoto K., Kikuchi S., Nishio K., Terashima I., Nakai M. Plant Cell 16:993-1007(2004) [PubMed: 15031412] [Abstract] Cited for: FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[6]	"Nfu2: a scaffold protein required for [4Fe-4S] and ferredoxin iron-sulphur cluster assembly in Arabidopsis chloroplasts." Touraine B., Boutin J.-P., Marion-Poll A., Briat J.-F., Peltier G., Lobreaux S. Plant J. 40:101-111(2004) [PubMed: 15361144] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AJ512934 mRNA. Translation: CAD55559.1.
AB024032 Genomic DNA. Translation: BAA97015.1. Sequence problems.
AF370353 mRNA. Translation: AAK44168.1.
AF428399 mRNA. Translation: AAL16167.1.
AY062984 mRNA. Translation: AAL34158.1.
AK118652 mRNA. Translation: BAC43248.1.

IPI

IPI00517598.

RefSeq

NP_568715.1.

UniGene

At.26219

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2Z51	X-ray	1.35	A	83-235	[»]

ModBase

Search...

Proteomic databases

PRIDE

Q93W20.

Genome annotation databases

GeneID

835057.

GenomeReviews

Gene locus AT5G49940 in contig BA000015_GR.

NMPDR

fig|3702.1.peg.26841.

Organism-specific databases

GeneFarm

3943. 402.

TAIR

At5g49940.

Phylogenomic databases

OMA

Q93W20. LHEIDGN.

Gene expression databases

ArrayExpress

Q93W20.

GermOnline

AT5G49940. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR001075. NIF_FeS_clus_asmbl_NifU_C.
[Graphical view]

Pfam

PF01106. NifU. 2 hits.
[Graphical view]

ProDom

PD002830. NifU_C. 2 hits.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Entry information

Entry name

NIFU2_ARATH

Accession

Primary (citable) accession number: Q93W20
Secondary accession number(s): Q9LTX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)