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Protein

Serine/threonine-protein kinase GRIK1

Gene

GRIK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates SnRK1.1/KIN10 and SnRK1.2/KIN11 by phosphorylation of their activation-loop 'Thr-198' and 'Thr-176', respectively. Required for the regulation by SnRK1 kinases of the transcription of a large set of genes, the modification the activity of metabolic enzymes, and the control of various nutrient-responsive cellular developmental processes.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated when autophosphorylated at Thr-154 and inactivated when phosphorylated at Ser-261 by SnRK1.1/KIN10.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei137ATPCurated1
Active sitei239Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi114 – 122ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  • protein autophosphorylation Source: UniProtKB
  • protein phosphorylation Source: UniProtKB
  • response to virus Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Host-virus interaction

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-442717. CREB phosphorylation through the activation of CaMKK.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase GRIK1 (EC:2.7.11.1)
Alternative name(s):
Protein GEMINIVIRUS REP INTERACTING KINASE 1
Short name:
Protein GRIK1
SnRK1-activating protein kinase 2
Short name:
AtSnAK2
Gene namesi
Name:GRIK1
Synonyms:SNAK2
Ordered Locus Names:At3g45240
ORF Names:F18N11, T14D3.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G45240.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Restricted to the nucleus in cells infected by geminivirus.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi137K → A: Abolishes autophosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004210341 – 396Serine/threonine-protein kinase GRIK1Add BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei154Phosphothreonine; by autocatalysis1 Publication1
Modified residuei261Phosphoserine; by KIN101 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ93V58.
PRIDEiQ93V58.

PTM databases

iPTMnetiQ93V58.
SwissPalmiQ93V58.

Expressioni

Tissue specificityi

Expressed in shoot apical meristem, leaf primordium and emerging petiole (at protein level).1 Publication

Developmental stagei

Highly expressed in young leaf and floral tissues but not expressed in mature tissues (at protein level).3 Publications

Inductioni

By geminivirus (TGMV, CaLCuV or BCTV) infection (at the protein level).3 Publications

Gene expression databases

ExpressionAtlasiQ93V58. baseline and differential.
GenevisibleiQ93V58. AT.

Interactioni

Subunit structurei

Associates with the SNF1-related protein kinase (SnRK) complex (By similarity). Interacts with AL1, a geminivirus (TGMV) protein essential for viral replication.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KIN10Q389972EBI-6399184,EBI-2107143

Protein-protein interaction databases

BioGridi8980. 2 interactors.
IntActiQ93V58. 3 interactors.
STRINGi3702.AT3G45240.1.

Structurei

3D structure databases

ProteinModelPortaliQ93V58.
SMRiQ93V58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 369Protein kinasePROSITE-ProRule annotationAdd BLAST262

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi51 – 61Poly-GluAdd BLAST11
Compositional biasi388 – 391Poly-Glu4

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0585. Eukaryota.
ENOG410YHHF. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ93V58.
KOiK07359.
OMAiNEYVREY.
OrthoDBiEOG09360CMZ.
PhylomeDBiQ93V58.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q93V58-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFCDSFAFAQ VMSCFGCFGG SERSRHSPNP YDDDTYSHDS GETSNPGGDD
60 70 80 90 100
EEGEEEEEVE ELSRSKRSEE ILKCKLQNGL VCRQFPVKET NKLTRGEDED
110 120 130 140 150
GNKTINEFVR ERKIGSGSYG KVVLYRSTVD DKHYAIKAFH KSHLSRLRVA
160 170 180 190 200
PSETAMGDVL REVMIMKTLE HPNIVNLIEV IDDPEFDDFY MVLEYVDGKW
210 220 230 240 250
AYDDSGPPGA LGEITARKYL RDVVAGLMYL HAHNVIHGDI KPDNLLVTST
260 270 280 290 300
GRVKIGDFSV SQVFKDDDDQ LRRSPGTPVF TAPECCLGIT YSGRSADTWA
310 320 330 340 350
VGVTLYCMIL GQYPFLGDTL QDTYDKIVHN PLIIPEGLNP RLRDLIEGLL
360 370 380 390
CKDPNQRMTL KAVAEHPWIT GEDGAISEYC CWCKRKAEEE EDQNHS
Length:396
Mass (Da):44,573
Last modified:December 1, 2001 - v1
Checksum:iF3C56641D73006B4
GO

Sequence cautioni

The sequence CAB72162 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM489731 mRNA. Translation: CAM32015.1.
AL132953 Genomic DNA. No translation available.
AL138649 Genomic DNA. Translation: CAB72162.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78007.1.
CP002686 Genomic DNA. Translation: AEE78008.1.
AY035070 mRNA. Translation: AAK59575.1.
AY056309 mRNA. Translation: AAL07158.1.
PIRiT47464.
RefSeqiNP_001030811.1. NM_001035734.2. [Q93V58-1]
NP_566876.3. NM_114393.4. [Q93V58-1]
UniGeneiAt.21488.

Genome annotation databases

EnsemblPlantsiAT3G45240.1; AT3G45240.1; AT3G45240. [Q93V58-1]
AT3G45240.2; AT3G45240.2; AT3G45240. [Q93V58-1]
GeneIDi823660.
GrameneiAT3G45240.1; AT3G45240.1; AT3G45240.
AT3G45240.2; AT3G45240.2; AT3G45240.
KEGGiath:AT3G45240.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM489731 mRNA. Translation: CAM32015.1.
AL132953 Genomic DNA. No translation available.
AL138649 Genomic DNA. Translation: CAB72162.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78007.1.
CP002686 Genomic DNA. Translation: AEE78008.1.
AY035070 mRNA. Translation: AAK59575.1.
AY056309 mRNA. Translation: AAL07158.1.
PIRiT47464.
RefSeqiNP_001030811.1. NM_001035734.2. [Q93V58-1]
NP_566876.3. NM_114393.4. [Q93V58-1]
UniGeneiAt.21488.

3D structure databases

ProteinModelPortaliQ93V58.
SMRiQ93V58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi8980. 2 interactors.
IntActiQ93V58. 3 interactors.
STRINGi3702.AT3G45240.1.

PTM databases

iPTMnetiQ93V58.
SwissPalmiQ93V58.

Proteomic databases

PaxDbiQ93V58.
PRIDEiQ93V58.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G45240.1; AT3G45240.1; AT3G45240. [Q93V58-1]
AT3G45240.2; AT3G45240.2; AT3G45240. [Q93V58-1]
GeneIDi823660.
GrameneiAT3G45240.1; AT3G45240.1; AT3G45240.
AT3G45240.2; AT3G45240.2; AT3G45240.
KEGGiath:AT3G45240.

Organism-specific databases

TAIRiAT3G45240.

Phylogenomic databases

eggNOGiKOG0585. Eukaryota.
ENOG410YHHF. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ93V58.
KOiK07359.
OMAiNEYVREY.
OrthoDBiEOG09360CMZ.
PhylomeDBiQ93V58.

Enzyme and pathway databases

ReactomeiR-ATH-442717. CREB phosphorylation through the activation of CaMKK.

Miscellaneous databases

PROiQ93V58.

Gene expression databases

ExpressionAtlasiQ93V58. baseline and differential.
GenevisibleiQ93V58. AT.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRIK1_ARATH
AccessioniPrimary (citable) accession number: Q93V58
Secondary accession number(s): Q9M1T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Functionally able to complement the yeast elm1 sak1 tos3 triple mutant.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.