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Protein

Guanine nucleotide-binding protein subunit gamma 2

Gene

GG2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Involved in the abscisic acid (ABA) and ethylene signaling pathways. Regulates basipetal transport of auxin (IAA) in roots and hypocotyls, and thus modulates root architecture (e.g. lateral root formation). The heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi probably by modulating cell wall-related genes expression; involved in resistance to Plectosphaerella cucumerina.6 Publications

GO - Molecular functioni

GO - Biological processi

  • basipetal auxin transport Source: TAIR
  • G-protein coupled receptor signaling pathway Source: InterPro
  • lateral root development Source: TAIR
  • protein palmitoylation Source: UniProtKB
  • protein prenylation Source: UniProtKB
  • seed germination Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit gamma 2
Alternative name(s):
Ggamma-subunit 2
Heterotrimeric G protein gamma-subunit 2
Short name:
AtAGG2
Gene namesi
Name:GG2
Synonyms:AGG2
Ordered Locus Names:At3g22942
ORF Names:F5N5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G22942.

Subcellular locationi

GO - Cellular componenti

  • heterotrimeric G-protein complex Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Hypersensitive to auxin-mediated induction of lateral roots, within the epidermis and/or cortex, attenuating basipetally transported auxin and graviresponsiveness. Enhanced sensitivity to glucose. Abnormal roots architecture. Enhanced susceptibility to necrotrophic and vascular pathogenic fungi, such as Plectosphaerella cucumerina associated with a disturbed expression of genes involved in cell wall metabolism (e.g. lower xylose content in cell walls).5 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi90 – 967KEAKRCG → NGGEGCR: Golgi and other internal membranes subcellular localization, probably by reduced lipidation. 1 Publication
Mutagenesisi95 – 951C → S: Loss of cell membrane attachment leading to Golgi and other internal membranes subcellular localization. 2 Publications
Mutagenesisi97 – 971C → S: No prenylation and loss of cell membrane attachment leading to cytoplasmic subcellular localization. 1 Publication
Mutagenesisi100 – 1001L → LTEFNH: Loss of cell membrane attachment leading to cytoplasmic subcellular localization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9797Guanine nucleotide-binding protein subunit gamma 2PRO_0000419815Add
BLAST
Propeptidei98 – 1003Removed in mature formPRO_0000419816

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources
Lipidationi95 – 951S-palmitoyl cysteine2 Publications
Modified residuei97 – 971Cysteine methyl esterCurated
Lipidationi97 – 971S-farnesyl cysteine1 Publication

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

PaxDbiQ93V47.
PRIDEiQ93V47.

PTM databases

iPTMnetiQ93V47.
SwissPalmiQ93V47.

Expressioni

Tissue specificityi

Mostly expressed in roots (excluded from the stele), seedlings (especially at the hypocotyl/root junction), floral stems, floral buds, flowers and siliques, and, to a lower extent, in leaves (restricted to guard cells). Also present in hydathods.3 Publications

Developmental stagei

In seedlings, first observed at the hypocotyl/root junction but later confined to the root, including root hairs. In flowers, expressed in the apex of stamen filaments at a very early developmental stage and disappeared before the flower opened. Not present in siliques.2 Publications

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. GPG1 interacts with the beta subunit GB1. The dimer GB1-GG2 interacts with NDL1, NDL2 and NDL3. Binds to NUDT7.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GB1P491772EBI-1751115,EBI-1632851

Protein-protein interaction databases

BioGridi10846. 4 interactions.
IntActiQ93V47. 2 interactions.
STRINGi3702.AT3G22942.1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 10080G protein gammaAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni90 – 967Regulates lipidation and cell membrane subcellular localization

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili19 – 5537Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 G protein gamma domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410J1I4. Eukaryota.
ENOG410YZU3. LUCA.
HOGENOMiHOG000238769.
InParanoidiQ93V47.
OMAiACKEMLS.
OrthoDBiEOG09360Z7R.
PhylomeDBiQ93V47.

Family and domain databases

InterProiIPR015898. G-protein_gamma-like_dom.
[Graphical view]
PfamiPF00631. G-gamma. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q93V47-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAGSSNSSG QLSGRVVDTR GKHRIQAELK RLEQEARFLE EELEQLEKMD
60 70 80 90 100
NASASCKEFL DSVDSKPDPL LPETTGPVNA TWDQWFEGPK EAKRCGCSIL
Length:100
Mass (Da):11,139
Last modified:December 1, 2001 - v1
Checksum:iF7CA80881064D4BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF347077 mRNA. Translation: AAK71536.1.
AF347078 Genomic DNA. Translation: AAK71537.1.
AP001300 Genomic DNA. No translation available.
CP002686 Genomic DNA. Translation: AEE76694.1.
BT024657 mRNA. Translation: ABD57482.1.
AK228306 mRNA. Translation: BAF00249.1.
RefSeqiNP_850746.1. NM_180415.2. [Q93V47-1]
UniGeneiAt.27127.
At.71143.

Genome annotation databases

EnsemblPlantsiAT3G22942.1; AT3G22942.1; AT3G22942. [Q93V47-1]
GeneIDi825532.
KEGGiath:AT3G22942.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF347077 mRNA. Translation: AAK71536.1.
AF347078 Genomic DNA. Translation: AAK71537.1.
AP001300 Genomic DNA. No translation available.
CP002686 Genomic DNA. Translation: AEE76694.1.
BT024657 mRNA. Translation: ABD57482.1.
AK228306 mRNA. Translation: BAF00249.1.
RefSeqiNP_850746.1. NM_180415.2. [Q93V47-1]
UniGeneiAt.27127.
At.71143.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10846. 4 interactions.
IntActiQ93V47. 2 interactions.
STRINGi3702.AT3G22942.1.

PTM databases

iPTMnetiQ93V47.
SwissPalmiQ93V47.

Proteomic databases

PaxDbiQ93V47.
PRIDEiQ93V47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G22942.1; AT3G22942.1; AT3G22942. [Q93V47-1]
GeneIDi825532.
KEGGiath:AT3G22942.

Organism-specific databases

TAIRiAT3G22942.

Phylogenomic databases

eggNOGiENOG410J1I4. Eukaryota.
ENOG410YZU3. LUCA.
HOGENOMiHOG000238769.
InParanoidiQ93V47.
OMAiACKEMLS.
OrthoDBiEOG09360Z7R.
PhylomeDBiQ93V47.

Miscellaneous databases

PROiQ93V47.

Family and domain databases

InterProiIPR015898. G-protein_gamma-like_dom.
[Graphical view]
PfamiPF00631. G-gamma. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGG2_ARATH
AccessioniPrimary (citable) accession number: Q93V47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.