Protease - Bacillus sp. KSM-KP43

Preferred order of sections

Names and origin

Protein names

Submitted name:
Protease EMBL BAB55674.2

Gene names

Name:

PROF EMBL BAB55674.2

Organism

Bacillus sp. KSM-KP43 EMBL BAB55674.2

Taxonomic identifier

109322 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Calcium PDB 1WMD PDB 1WME PDB 1WMF Metal-binding PDB 1WMD PDB 1WME PDB 1WMF
Molecular function	Hydrolase Protease EMBL BAB55674.2
Technical term	3D-structure PDB 1WMD PDB 1WME PDB 1WMF
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

392

1

Calcium 1 PDB 1WMD PDB 1WME PDB 1WMF

Metal binding

400

1

Calcium 1; via carbonyl oxygen PDB 1WMD PDB 1WME PDB 1WMF

Metal binding

403

1

Calcium 1 PDB 1WMD PDB 1WME PDB 1WMF

Metal binding

407

1

Calcium 1; via pros nitrogen PDB 1WMD PDB 1WME PDB 1WMF

Metal binding

573

1

Calcium 2 PDB 1WMD PDB 1WME PDB 1WMF

Metal binding

574

1

Calcium 2; via carbonyl oxygen PDB 1WMD PDB 1WME PDB 1WMF

Metal binding

575

1

Calcium 2 PDB 1WMD PDB 1WME PDB 1WMF

Metal binding

590

1

Calcium 3 PDB 1WMD PDB 1WME PDB 1WMF

Metal binding

592

1

Calcium 3 PDB 1WMD PDB 1WME PDB 1WMF

Metal binding

594

1

Calcium 3; via carbonyl oxygen PDB 1WMD PDB 1WME PDB 1WMF

Metal binding

597

1

Calcium 3 PDB 1WMD PDB 1WME PDB 1WMF

Metal binding

598

1

Calcium 3 PDB 1WMD PDB 1WME PDB 1WMF

Metal binding

600

1

Calcium 2; via carbonyl oxygen PDB 1WMD PDB 1WME PDB 1WMF

Metal binding

606

1

Calcium 2 PDB 1WMD PDB 1WME PDB 1WMF

Sequences

Sequence

Length

Mass (Da)

Tools

Q93UV9 [UniParc].

Last modified October 1, 2002. Version 2.
Checksum: 4BBAF77E9D592C15
Show »

FASTA

640

67,991

        10         20         30         40         50         60 
MRKKKKVFLS VLSAAAILST VALSNPSAGG ARNFDLDFKG IQTTTDAKGF SKQGQTGAAA 

        70         80         90        100        110        120 
FLVESENVKL PKGLQKKLET VPANNKLHII QFNGPILEET KQQLEKTGAK ILDYIPDYAY 

       130        140        150        160        170        180 
IVEYEGDVKS ATSTIEHVES VEPYLPIYRI DPQLFTKGAS ELVKAVALDT KQKNKEVQLR 

       190        200        210        220        230        240 
GIEQIAQFAI SNDVLYITAK PEYKVMNDVA RGIVKADVAQ SSYGLYGQGQ IVAVADTGLD 

       250        260        270        280        290        300 
TGRNDSSMHE AFRGKITALY ALGRTNNAND TNGHGTHVAG SVLGNGSTNK GMAPQANLVF 

       310        320        330        340        350        360 
QSIMDSGGGL GGLPSNLQTL FSQAYSAGAR IHTNSWGAAV NGAYTTDSRN VDDYVRKNDM 

       370        380        390        400        410        420 
TILFAAGNEG PNGGTISAPG TAKNAITVGA TENLRPSFGS YADNINHVAQ FSSRGPTKDG 

       430        440        450        460        470        480 
RIKPDVMAPG TFILSARSSL APDSSFWANH DSKYAYMGGT SMATPIVAGN VAQLREHFVK 

       490        500        510        520        530        540 
NRGITPKPSL LKAALIAGAA DIGLGYPNGN QGWGRVTLDK SLNVAYVNES SSLSTSQKAT 

       550        560        570        580        590        600 
YSFTATAGKP LKISLVWSDA PASTTASVTL VNDLDLVITA PNGTQYVGND FTSPYNDNWD 

       610        620        630        640 
GRNNVENVFI NAPQSGTYTI EVQAYNVPVG PQTFSLAIVN

« Hide

References

[1]	"new protease." Itoh S., Saeki K. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: KP43 EMBL BAB55674.2.
[2]	"The crystal structure of an oxidatively stable subtilisin-like alkaline serine protease, KP-43, with a C-terminal beta-barrel domain." Nonaka T., Fujihashi M., Kita A., Saeki K., Ito S., Horikoshi K., Miki K. J. Biol. Chem. 279:47344-47351(2004) [PubMed: 15342641] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) OF 207-640 IN COMPLEX WITH CALCIUM.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB051423 Genomic DNA. Translation: BAB55674.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WMD	X-ray	1.30	A	207-640	[»]
1WME	X-ray	1.50	A	207-640	[»]
1WMF	X-ray	1.73	A	207-640	[»]

ProteinModelPortal

Q93UV9.

SMR

Q93UV9. Positions 207-640.

ModBase

Search...

Protein family/group databases

MEROPS

S08.123.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008979. Galactose-bd-like.
IPR007280. Peptidase_C_arc/bac.
IPR000209. Peptidase_S8/S53.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
[Graphical view]

Gene3D

G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.

PANTHER

PTHR10795. SubtilSerProt. 1 hit.

Pfam

PF00082. Peptidase_S8. 1 hit.
PF04151. PPC. 1 hit.
[Graphical view]

PRINTS

PR00723. SUBTILISIN.

SUPFAM

SSF49785. Gal_bind_like. 1 hit.
SSF52743. Pept_S8_S53. 1 hit.

PROSITE

PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q93UV9_9BACI

Accession

Primary (citable) accession number: Q93UV9

Entry history

Integrated into UniProtKB/TrEMBL:	December 1, 2001
Last sequence update:	October 1, 2002
Last modified:	November 16, 2011
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)