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Protein

Phosphoheptose isomerase

Gene

gmhA

Organism
Burkholderia pseudomallei (strain K96243)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.1 Publication

Catalytic activityi

D-sedoheptulose 7-phosphate = D-glycero-D-manno-heptose 7-phosphate.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Pathwayi: D-glycero-D-manno-heptose 7-phosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Phosphoheptose isomerase (gmhA)
This subpathway is part of the pathway D-glycero-D-manno-heptose 7-phosphate biosynthesis, which is itself part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate, the pathway D-glycero-D-manno-heptose 7-phosphate biosynthesis and in Carbohydrate biosynthesis.

Pathwayi: capsule polysaccharide biosynthesis

This protein is involved in the pathway capsule polysaccharide biosynthesis, which is part of Capsule biogenesis.
View all proteins of this organism that are known to be involved in the pathway capsule polysaccharide biosynthesis and in Capsule biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi64Zinc1 Publication1
Metal bindingi68Zinc1 Publication1
Binding sitei68Substrate1
Binding sitei128Substrate1
Metal bindingi175Zinc1 Publication1
Binding sitei175Substrate1
Metal bindingi183Zinc1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Capsule biogenesis/degradation, Carbohydrate metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi5.3.1.28. 1031.
UniPathwayiUPA00041; UER00436.
UPA00934.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoheptose isomerase (EC:5.3.1.28)
Alternative name(s):
Sedoheptulose 7-phosphate isomerase
Gene namesi
Name:gmhA
Ordered Locus Names:BPSL2795
OrganismiBurkholderia pseudomallei (strain K96243)
Taxonomic identifieri272560 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000000605 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61D → A: Less than 6% of wild-type activity. 1 Publication1
Mutagenesisi64H → Q: Less than 10% of wild-type activity. 1 Publication1
Mutagenesisi68E → Q: No activity. 1 Publication1
Mutagenesisi98D → N: No activity. 1 Publication1
Mutagenesisi124T → A: No activity. 1 Publication1
Mutagenesisi175Q → E: No activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001365211 – 197Phosphoheptose isomeraseAdd BLAST197

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi272560.BPSL2795.

Structurei

Secondary structure

1197
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 24Combined sources21
Helixi27 – 45Combined sources19
Beta strandi50 – 53Combined sources4
Helixi57 – 70Combined sources14
Beta strandi73 – 75Combined sources3
Beta strandi82 – 84Combined sources3
Helixi89 – 99Combined sources11
Helixi101 – 103Combined sources3
Helixi106 – 112Combined sources7
Beta strandi118 – 122Combined sources5
Beta strandi124 – 126Combined sources3
Helixi129 – 140Combined sources12
Beta strandi144 – 149Combined sources6
Helixi156 – 159Combined sources4
Beta strandi161 – 165Combined sources5
Helixi171 – 194Combined sources24

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X3YX-ray2.40A/B/C/D/E/F/G/H1-197[»]
2XBLX-ray1.62A/B/C/D1-197[»]
ProteinModelPortaliQ93UJ2.
SMRiQ93UJ2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ93UJ2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 197SISAdd BLAST158

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni55 – 57Substrate binding3
Regioni97 – 98Substrate binding2
Regioni123 – 125Substrate binding3

Sequence similaritiesi

Belongs to the SIS family. GmhA subfamily.Curated
Contains 1 SIS domain.Curated

Phylogenomic databases

eggNOGiENOG4105F55. Bacteria.
COG0279. LUCA.
HOGENOMiHOG000237571.
KOiK03271.
OMAiHEICIRV.

Family and domain databases

HAMAPiMF_00067. GmhA. 1 hit.
InterProiIPR004515. Phosphoheptose_Isoase.
IPR001347. SIS.
[Graphical view]
PfamiPF13580. SIS_2. 1 hit.
[Graphical view]
PROSITEiPS51464. SIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q93UJ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENRELTYIT NSIAEAQRVM AAMLADERLL ATVRKVADAC IASIAQGGKV
60 70 80 90 100
LLAGNGGSAA DAQHIAGEFV SRFAFDRPGL PAVALTTDTS ILTAIGNDYG
110 120 130 140 150
YEKLFSRQVQ ALGNEGDVLI GYSTSGKSPN ILAAFREAKA KGMTCVGFTG
160 170 180 190
NRGGEMRELC DLLLEVPSAD TPKIQEGHLV LGHIVCGLVE HSIFGKQ
Length:197
Mass (Da):20,813
Last modified:November 23, 2004 - v2
Checksum:i22A249C834AC585B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34R → Q in AAK49808 (PubMed:11119486).Curated1
Sequence conflicti115E → K in AAK49808 (PubMed:11119486).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF228583 Genomic DNA. Translation: AAK49808.1.
BX571965 Genomic DNA. Translation: CAH36804.1.
RefSeqiWP_011205222.1. NC_006350.1.
YP_109390.1. NC_006350.1.

Genome annotation databases

EnsemblBacteriaiCAH36804; CAH36804; BPSL2795.
GeneIDi3092932.
KEGGibps:BPSL2795.
PATRICi19265991. VBIBurPse99623_3191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF228583 Genomic DNA. Translation: AAK49808.1.
BX571965 Genomic DNA. Translation: CAH36804.1.
RefSeqiWP_011205222.1. NC_006350.1.
YP_109390.1. NC_006350.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X3YX-ray2.40A/B/C/D/E/F/G/H1-197[»]
2XBLX-ray1.62A/B/C/D1-197[»]
ProteinModelPortaliQ93UJ2.
SMRiQ93UJ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272560.BPSL2795.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH36804; CAH36804; BPSL2795.
GeneIDi3092932.
KEGGibps:BPSL2795.
PATRICi19265991. VBIBurPse99623_3191.

Phylogenomic databases

eggNOGiENOG4105F55. Bacteria.
COG0279. LUCA.
HOGENOMiHOG000237571.
KOiK03271.
OMAiHEICIRV.

Enzyme and pathway databases

UniPathwayiUPA00041; UER00436.
UPA00934.
BRENDAi5.3.1.28. 1031.

Miscellaneous databases

EvolutionaryTraceiQ93UJ2.

Family and domain databases

HAMAPiMF_00067. GmhA. 1 hit.
InterProiIPR004515. Phosphoheptose_Isoase.
IPR001347. SIS.
[Graphical view]
PfamiPF13580. SIS_2. 1 hit.
[Graphical view]
PROSITEiPS51464. SIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGMHA_BURPS
AccessioniPrimary (citable) accession number: Q93UJ2
Secondary accession number(s): Q63R78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: November 23, 2004
Last modified: November 2, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.