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Protein

Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylase

Gene

asD

Organism
Comamonas testosteroni (Pseudomonas testosteroni)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme that has both L-aspartate decarboxylase and transaminase activity.2 Publications

Catalytic activityi

L-aspartate = L-alanine + CO2.
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.

Cofactori

pyridoxal 5'-phosphate1 Publication

Kineticsi

  1. KM=9 mM for L-aspartate1 Publication

    pH dependencei

    Optimum pH is 5.1 Publication

    Temperature dependencei

    Optimum temperature is 45 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei115Aspartate; via amide nitrogenBy similarity1
    Binding sitei256AspartateBy similarity1
    Binding sitei497AspartateBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    • alanine biosynthetic process Source: UniProtKB
    • aspartate metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Aminotransferase, Decarboxylase, Lyase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylase (EC:2.6.1.1, EC:4.1.1.12)
    Alternative name(s):
    Aspartate 4-decarboxylase
    Short name:
    ASD
    Short name:
    AsdA
    Gene namesi
    Name:asD
    OrganismiComamonas testosteroni (Pseudomonas testosteroni)
    Taxonomic identifieri285 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeComamonas

    Pathology & Biotechi

    Biotechnological usei

    Is highly specific for L-aspartic acid, and so can be used to produce L-alanine and D-aspartic acid from DL-aspartic acid.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi134Y → F: Slightly reduced activity. 1 Publication1
    Mutagenesisi315K → A: Slightly reduced activity. 1 Publication1
    Mutagenesisi487R → A: Loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004191231 – 533Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylaseAdd BLAST533

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei315N6-(pyridoxal phosphate)lysine1

    Interactioni

    Subunit structurei

    Homododecamer.1 Publication

    Protein-protein interaction databases

    DIPiDIP-48316N.
    STRINGi399795.CtesDRAFT_PD2303.

    Structurei

    Secondary structure

    1533
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi3 – 6Combined sources4
    Helixi13 – 23Combined sources11
    Helixi27 – 35Combined sources9
    Beta strandi41 – 44Combined sources4
    Helixi46 – 63Combined sources18
    Beta strandi74 – 76Combined sources3
    Helixi83 – 93Combined sources11
    Turni94 – 96Combined sources3
    Helixi98 – 112Combined sources15
    Helixi118 – 130Combined sources13
    Beta strandi136 – 139Combined sources4
    Helixi142 – 155Combined sources14
    Helixi163 – 165Combined sources3
    Beta strandi166 – 172Combined sources7
    Helixi173 – 187Combined sources15
    Beta strandi196 – 202Combined sources7
    Helixi205 – 212Combined sources8
    Turni214 – 216Combined sources3
    Beta strandi219 – 224Combined sources6
    Helixi227 – 229Combined sources3
    Helixi235 – 238Combined sources4
    Helixi239 – 242Combined sources4
    Beta strandi246 – 254Combined sources9
    Beta strandi256 – 258Combined sources3
    Helixi264 – 276Combined sources13
    Beta strandi282 – 286Combined sources5
    Helixi290 – 292Combined sources3
    Beta strandi293 – 295Combined sources3
    Helixi299 – 302Combined sources4
    Helixi304 – 306Combined sources3
    Beta strandi307 – 313Combined sources7
    Turni314 – 318Combined sources5
    Helixi320 – 322Combined sources3
    Beta strandi324 – 332Combined sources9
    Helixi334 – 340Combined sources7
    Helixi344 – 354Combined sources11
    Turni355 – 357Combined sources3
    Helixi361 – 363Combined sources3
    Helixi366 – 373Combined sources8
    Turni374 – 379Combined sources6
    Helixi380 – 382Combined sources3
    Helixi387 – 402Combined sources16
    Helixi407 – 424Combined sources18
    Helixi425 – 427Combined sources3
    Beta strandi440 – 445Combined sources6
    Helixi446 – 454Combined sources9
    Helixi456 – 465Combined sources10
    Helixi468 – 479Combined sources12
    Beta strandi484 – 487Combined sources4
    Beta strandi495 – 503Combined sources9
    Helixi505 – 525Combined sources21
    Helixi529 – 533Combined sources5

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2ZY3X-ray2.50A/B/C/D/E/F1-533[»]
    2ZY4X-ray2.00A/B/C/D/E/F1-533[»]
    2ZY5X-ray2.65A/B/C/D/E/F1-533[»]
    ProteinModelPortaliQ93QX0.
    SMRiQ93QX0.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ93QX0.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105CEB. Bacteria.
    COG0436. LUCA.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR022518. Aspartate_4-decarboxylase.
    IPR004838. NHTrfase_class1_PyrdxlP-BS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 2 hits.
    TIGRFAMsiTIGR03801. asp_4_decarbox. 1 hit.
    PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q93QX0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSKDYQSLAK LSPFELKDEL IKIASSDGNR LMLNAGRGNP NFLATTPRRA
    60 70 80 90 100
    FFRLGLFAAA ESELSYSYMT TVGVGGLAKI DGIEGRFERY IAENRDQEGV
    110 120 130 140 150
    RFLGKSLSYV RDQLGLDPAA FLHEMVDGIL GCNYPVPPRM LNISEKIVRQ
    160 170 180 190 200
    YIIREMGADA IPSESVNLFA VEGGTAAMAY IFESLKLNGL LKAGDKVAIG
    210 220 230 240 250
    MPVFTPYIEI PELAQYALEE VAINADPSLN WQYPDSELDK LKDPAIKIFF
    260 270 280 290 300
    CVNPSNPPSV KMDQRSLERV RNIVAEHRPD LMILTDDVYG TFADDFQSLF
    310 320 330 340 350
    AICPENTLLV YSFSKYFGAT GWRLGVVAAH QQNVFDLALD KLQESEKVAL
    360 370 380 390 400
    DHRYRSLLPD VRSLKFIDRL VADSRAVALN HTAGLSTPQQ VQMALFSLFA
    410 420 430 440 450
    LMDEADEYKH TLKQLIRRRE TTLYRELGMP PLRDENAVDY YTLIDLQDVT
    460 470 480 490 500
    AKLYGEAFSE WAVKQSSTGD MLFRIADETG IVLLPGRGFG SNRPSGRASL
    510 520 530
    ANLNEYEYAA IGRALRKMAD ELYAEYSGQA QNL
    Length:533
    Mass (Da):59,584
    Last modified:December 1, 2001 - v1
    Checksum:iB6E0042383A51113
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF168368 Genomic DNA. Translation: AAK58507.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF168368 Genomic DNA. Translation: AAK58507.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2ZY3X-ray2.50A/B/C/D/E/F1-533[»]
    2ZY4X-ray2.00A/B/C/D/E/F1-533[»]
    2ZY5X-ray2.65A/B/C/D/E/F1-533[»]
    ProteinModelPortaliQ93QX0.
    SMRiQ93QX0.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    DIPiDIP-48316N.
    STRINGi399795.CtesDRAFT_PD2303.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Phylogenomic databases

    eggNOGiENOG4105CEB. Bacteria.
    COG0436. LUCA.

    Miscellaneous databases

    EvolutionaryTraceiQ93QX0.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR022518. Aspartate_4-decarboxylase.
    IPR004838. NHTrfase_class1_PyrdxlP-BS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 2 hits.
    TIGRFAMsiTIGR03801. asp_4_decarbox. 1 hit.
    PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiASDA_COMTE
    AccessioniPrimary (citable) accession number: Q93QX0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: December 1, 2001
    Last modified: November 2, 2016
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.