Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Monofunctional glycosyltransferase

Gene

mgt

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Peptidoglycan polymerase that catalyzes glycan chain elongation using lipid-linked disaccharide-pentapeptide as the substrate.UniRule annotation1 Publication

Catalytic activityi

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.UniRule annotation1 Publication

Enzyme regulationi

Activity increases 3-fold at pH 6.1. Inhibited by moenomycin A.1 Publication

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationCurated
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15458.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Monofunctional glycosyltransferase1 PublicationUniRule annotation (EC:2.4.1.129UniRule annotation1 Publication)
Short name:
MGT1 PublicationUniRule annotation
Alternative name(s):
Peptidoglycan TGaseUniRule annotationCurated
Gene namesi
Name:mgt1 PublicationUniRule annotation
Ordered Locus Names:SAOUHSC_02012
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation1 Publication; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei46 – 66HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000831521 – 269Monofunctional glycosyltransferaseAdd BLAST269

Interactioni

Protein-protein interaction databases

STRINGi93061.SAOUHSC_02012.

Structurei

3D structure databases

ProteinModelPortaliQ93Q23.
SMRiQ93Q23.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 51 family.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ4. Bacteria.
COG0744. LUCA.
HOGENOMiHOG000288116.
KOiK04478.
OMAiGSDQYTI.

Family and domain databases

Gene3Di1.10.3810.10. 1 hit.
HAMAPiMF_01434. MGT. 1 hit.
InterProiView protein in InterPro
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR022978. Monofunct_glyco_trans.
PfamiView protein in Pfam
PF00912. Transgly. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.

Sequencei

Sequence statusi: Complete.

Q93Q23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSDRYSNS NEHFEHMKHE PHYNTYYQPV GKPPKKKKSK RILLKILLTI
60 70 80 90 100
LIIIALFIGI MYFLSTRDNV DELRKIENKS SFVSADNMPE YVKGAFISME
110 120 130 140 150
DERFYNHHGF DLKGTTRALF STISDRDVQG GSTITQQVVK NYFYDNDRSF
160 170 180 190 200
TRKVKELFVA HRVEKQYNKN EILSFYLNNI YFGDNQYTLE GAANHYFGTT
210 220 230 240 250
VNKNSTTMSH ITVLQSAILA SKVNAPSVYN INNMSENFTQ RVSTNLEKMK
260
QQNYINETQY QQAMSQLNR
Length:269
Mass (Da):31,460
Last modified:July 11, 2006 - v2
Checksum:iC0F65B9F5CAB8761
GO

Sequence cautioni

The sequence AAA18515 differs from that shown. Reason: Erroneous termination at position 150. Translated as Phe. May be due to a cloning artifact.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti257 – 258Missing in AAK83040 (PubMed:11466281).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF287468 Genomic DNA. Translation: AAK83040.1.
L19300 Unassigned DNA. Translation: AAA18515.1. Sequence problems.
CP000253 Genomic DNA. Translation: ABD31068.1.
RefSeqiWP_000830380.1. NC_007795.1.
YP_500509.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD31068; ABD31068; SAOUHSC_02012.
GeneIDi28380483.
3920466.
KEGGisao:SAOUHSC_02012.
PATRICi19581411. VBIStaAur99865_1827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF287468 Genomic DNA. Translation: AAK83040.1.
L19300 Unassigned DNA. Translation: AAA18515.1. Sequence problems.
CP000253 Genomic DNA. Translation: ABD31068.1.
RefSeqiWP_000830380.1. NC_007795.1.
YP_500509.1. NC_007795.1.

3D structure databases

ProteinModelPortaliQ93Q23.
SMRiQ93Q23.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93061.SAOUHSC_02012.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD31068; ABD31068; SAOUHSC_02012.
GeneIDi28380483.
3920466.
KEGGisao:SAOUHSC_02012.
PATRICi19581411. VBIStaAur99865_1827.

Phylogenomic databases

eggNOGiENOG4105BZ4. Bacteria.
COG0744. LUCA.
HOGENOMiHOG000288116.
KOiK04478.
OMAiGSDQYTI.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciMetaCyc:MONOMER-15458.

Family and domain databases

Gene3Di1.10.3810.10. 1 hit.
HAMAPiMF_01434. MGT. 1 hit.
InterProiView protein in InterPro
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR022978. Monofunct_glyco_trans.
PfamiView protein in Pfam
PF00912. Transgly. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMGT_STAA8
AccessioniPrimary (citable) accession number: Q93Q23
Secondary accession number(s): Q2G2G8, Q53720
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 11, 2006
Last modified: February 15, 2017
This is version 88 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.