Q93Q23 (MGT_STAA8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Monofunctional glycosyltransferase
Short name=MGT
EC=2.4.-.-

Alternative name(s):
Peptidoglycan TGase

Gene names

Name:	mgt
Ordered Locus Names:	SAOUHSC_02012

Organism

Staphylococcus aureus (strain NCTC 8325) [Reference proteome] [HAMAP]

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the biosynthesis of cell wall peptidoglycan. Responsible for the elongation of the glycan strands using lipid-linked disaccharide-pentapeptide as the substrate. Ref.1
Enzyme regulation	Activity increases 3-fold at pH 6.1. Inhibited by moenomycin A. Ref.1
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_01434
Subcellular location	Cell membrane; Single-pass membrane protein HAMAP-Rule MF_01434.
Sequence similarities	Belongs to the glycosyltransferase 51 family.
Sequence caution	The sequence AAA18515.1 differs from that shown. Reason: Erroneous termination at position 150. Translated as Phe. May be due to a cloning artifact.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan-based cell wall Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Monofunctional glycosyltransferase HAMAP-Rule MF_01434		PRO_0000083152
Regions
	Transmembrane	46 – 66	21	Helical; Potential
Experimental info
	Sequence conflict	257 – 258	2	Missing in AAK83040. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q93Q23 [UniParc]. Last modified July 11, 2006. Version 2. Checksum: C0F65B9F5CAB8761	FASTA	269	31,460
	10 20 30 40 50 60 MKRSDRYSNS NEHFEHMKHE PHYNTYYQPV GKPPKKKKSK RILLKILLTI LIIIALFIGI 70 80 90 100 110 120 MYFLSTRDNV DELRKIENKS SFVSADNMPE YVKGAFISME DERFYNHHGF DLKGTTRALF 130 140 150 160 170 180 STISDRDVQG GSTITQQVVK NYFYDNDRSF TRKVKELFVA HRVEKQYNKN EILSFYLNNI 190 200 210 220 230 240 YFGDNQYTLE GAANHYFGTT VNKNSTTMSH ITVLQSAILA SKVNAPSVYN INNMSENFTQ 250 260 RVSTNLEKMK QQNYINETQY QQAMSQLNR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a monofunctional glycosyltransferase from Staphylococcus aureus." Wang Q.M., Peery R.B., Johnson R.B., Alborn W.E., Yeh W.-K., Skatrud P.L. J. Bacteriol. 183:4779-4785(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15, FUNCTION, ENZYME REGULATION.
[2]	"Sequence analysis of the region downstream from a peptidoglycan hydrolase-encoding gene from Staphylococcus aureus NCTC8325." Borchardt S.A., Babwah A.V., Jayaswal R.K. Gene 137:253-258(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 8325.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF287468 Genomic DNA. Translation: AAK83040.1. L19300 Unassigned DNA. Translation: AAA18515.1. Sequence problems. CP000253 Genomic DNA. Translation: ABD31068.1.
RefSeq	YP_500509.1. NC_007795.1.
3D structure databases
ProteinModelPortal	Q93Q23.
SMR	Q93Q23. Positions 61-269.
ModBase	Search...
Protein-protein interaction databases
STRING	93061.SAOUHSC_02012.
Protein family/group databases
CAZy	GT51. Glycosyltransferase Family 51.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD31068; ABD31068; SAOUHSC_02012.
GeneID	3920466.
KEGG	sao:SAOUHSC_02012.
PATRIC	19581411. VBIStaAur99865_1827.
Phylogenomic databases
eggNOG	COG0744.
HOGENOM	HOG000288116.
KO	K04478.
OMA	YISHDEY.
ProtClustDB	PRK13481.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15458. SAUR93061:GIWJ-1962-MONOMER.
UniPathway	UPA00219.
Family and domain databases
HAMAP	MF_01434. MGT.
InterPro	IPR001264. Glyco_trans_51. IPR022978. Monofunct_glyco_trans. [Graphical view]
Pfam	PF00912. Transgly. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGT_STAA8

Accession

Primary (citable) accession number: Q93Q23
Secondary accession number(s): Q2G2G8, Q53720

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	July 11, 2006
Last modified:	May 1, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program