Q93Q17 (AEXT_AERSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-ribosyltransferase toxin AexT
EC=2.4.2.-

Alternative name(s):
Exoenzyme T

Gene names

Name:

aexT

Organism

Aeromonas salmonicida

Taxonomic identifier

645 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Directly involved in the toxicity for RTG-2 (rainbow trout gonad) fish cells.
Subcellular location	Secreted. Note: Translocated into the cytosol of fish cells via a type III secretion system.
Miscellaneous	Although the gene exists in strain ATCC 33658, it is not expressed.
Sequence similarities	Belongs to the YopE family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Glycosyltransferase Toxin Transferase
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro positive regulation of GTPase activity Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTPase activator activity Inferred from electronic annotation. Source: InterPro transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	ADP-ribosyltransferase toxin AexT		PRO_0000064470

Sequences

	Sequence		Length	Mass (Da)
	Q93Q17 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 95069F91E8820877	FASTA	475	50,105
	10 20 30 40 50 60 MQIQANTVGT QAVAHHSDAT TGVGRMGQME ARQVATGQDA ILLGSRSEPQ KGQGLLSRLG 70 80 90 100 110 120 AQLARPFVAI KEWISNLLGT DKRAAAPKAQ TAVSPEDLQR LMKQAAFGSS LGGFAKADVL 130 140 150 160 170 180 NNITGEQLGK DHASLATGNG PLRSLCTALQ AVVIGSQQPQ LRELATGLLA RPIAGIPLQQ 190 200 210 220 230 240 WGSVGGKVTE LLTSAPPELL KEAMSQLHTA MGEVADLQRA VKAEVAGEPA RSATTAAAVA 250 260 270 280 290 300 PLQSGESEVN VEPADKALAE GLQEQFGLEA EQYLGEQPHG TYSDAEVMAL GLYTNGEYQH 310 320 330 340 350 360 LNRSLRQEKQ LDAGQALIDQ GMSTAFEKST PTEQLIKTFR GTHGGDAFNE VAEGQVGHDV 370 380 390 400 410 420 AYLSTSRDPK VATNFGGSGS ISTIFGRSGI DVSDISVEGD EQEILYNKET DMRVLLSAKD 430 440 450 460 470 ERGVTRRVLE EASLGEQSGH SKGLLDGLDL ARGAGGADKP QEQDIRLKMR GLDLA

References

[1]	"Characterization of an ADP-ribosyltransferase toxin (AexT) from Aeromonas salmonicida subsp. salmonicida." Braun M., Stuber K., Schlatter Y., Wahli T., Kuhnert P., Frey J. J. Bacteriol. 184:1851-1858(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TOXICITY. Strain: ATCC 33658 / DSM 19634 / JCM 7874 / NCIMB 1102 and JF2267.
[2]	"The ADP-ribosylating toxin, AexT, from Aeromonas salmonicida subsp. salmonicida is translocated via a type III secretion pathway." Burr S.E., Stuber K., Frey J. J. Bacteriol. 185:6583-6591(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SECRETION VIA TYPE III SECRETION PATHWAY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF288366 Genomic DNA. Translation: AAK83052.1. AJ578475 Genomic DNA. Translation: CAE17664.1.
3D structure databases
ProteinModelPortal	Q93Q17.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.20.120.260. 1 hit.
InterPro	IPR003540. ADP-ribosyltransferase. IPR014773. Uncharacterised_dom_YopE. IPR003537. Vir_YopE. [Graphical view]
Pfam	PF03496. ADPrib_exo_Tox. 1 hit. PF03545. YopE. 1 hit. [Graphical view]
PRINTS	PR01372. YERSINIAYOPE.
SUPFAM	SSF47233. Vir_YopE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AEXT_AERSA

Accession

Primary (citable) accession number: Q93Q17

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	December 1, 2001
Last modified:	March 6, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program