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Q93PS7

- PDCH_COMTE

UniProt

Q93PS7 - PDCH_COMTE

Protein

2-pyrone-4,6-dicarbaxylate hydrolase

Gene
N/A
Organism
Comamonas testosteroni (Pseudomonas testosteroni)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 30 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Involved in the meta fission degradative pathway of protocatechuate. Catalyzes the reversible hydrolysis of 2-pyrone-4,6-dicarboxylate (PDC) to a mixture of 4-carboxy-2-hydroxymuconate (CHM) and 4-oxalomesaconate (OMA).1 Publication

    Catalytic activityi

    2-oxo-2H-pyran-4,6-dicarboxylate + H2O = (1E)-4-oxobut-1-ene-1,2,4-tricarboxylate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501SubstrateBy similarity
    Binding sitei78 – 781SubstrateBy similarity
    Binding sitei125 – 1251SubstrateBy similarity
    Binding sitei131 – 1311SubstrateBy similarity
    Binding sitei158 – 1581SubstrateBy similarity
    Binding sitei182 – 1821SubstrateBy similarity
    Active sitei258 – 2581By similarity
    Binding sitei263 – 2631SubstrateBy similarity

    GO - Molecular functioni

    1. 2-pyrone-4,6-dicarboxylate lactonase activity Source: UniProtKB

    GO - Biological processi

    1. protocatechuate catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-3463.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-pyrone-4,6-dicarbaxylate hydrolase
    Alternative name(s):
    2-pyrone-4,6-dicarboxylate lactonohydrolase
    PDC hydrolase (EC:3.1.1.57)
    OrganismiComamonas testosteroni (Pseudomonas testosteroni)
    Taxonomic identifieri285 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeComamonas

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene completely lose the ability to grow on protocatechuate (PCA) and accumulated PDC.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3053052-pyrone-4,6-dicarbaxylate hydrolasePRO_0000422784Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ93PS7.
    SMRiQ93PS7. Positions 8-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni34 – 341Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the PDC hydrolase family.Curated

    Family and domain databases

    InterProiIPR006992. Amidohydro_2.
    [Graphical view]
    PfamiPF04909. Amidohydro_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q93PS7-1 [UniParc]FASTAAdd to Basket

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    MSQFEKTPGW LDWYANPSKP QFKLPAGAVD AHCHVFGPGN EFPFAPERKY    50
    TPCDASKAQL YALRDHLGFA RNVVVQATCH GADNRAMVDA CKSSGGKARG 100
    VATVKRSISD AELQQLHDAG VRGVRFNFVK RLVDFTPKDE LMEIAGRIAK 150
    LGWHVVIYFE AVDLPELWDF FTALPTTVVV DHMGRPDVTK GVDSEEFALF 200
    LKFMREHQNV WSKVSCPERL SVTGPKALNG EQNAYRDVVP FARRVVEEFP 250
    DRVLWGTDWP HPNLKDHMPD DGLLVDFIPH IAPTAELQQK LLVDNPMRLY 300
    WPEEV 305
    Length:305
    Mass (Da):34,451
    Last modified:December 1, 2001 - v1
    Checksum:i2167B504424FDDDF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF305325 Genomic DNA. Translation: AAK73571.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF305325 Genomic DNA. Translation: AAK73571.1 .

    3D structure databases

    ProteinModelPortali Q93PS7.
    SMRi Q93PS7. Positions 8-305.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-3463.

    Family and domain databases

    InterProi IPR006992. Amidohydro_2.
    [Graphical view ]
    Pfami PF04909. Amidohydro_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate."
      Providenti M.A., Mampel J., MacSween S., Cook A.M., Wyndham R.C.
      Microbiology 147:2157-2167(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BR6020.
    2. "Characterization of the protocatechuate 4,5-cleavage pathway operon in Comamonas sp. strain E6 and discovery of a novel pathway gene."
      Kamimura N., Aoyama T., Yoshida R., Takahashi K., Kasai D., Abe T., Mase K., Katayama Y., Fukuda M., Masai E.
      Appl. Environ. Microbiol. 76:8093-8101(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiPDCH_COMTE
    AccessioniPrimary (citable) accession number: Q93PS7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 26, 2013
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 30 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3