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Q93PS7

- PDCH_COMTE

UniProt

Q93PS7 - PDCH_COMTE

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Protein

2-pyrone-4,6-dicarbaxylate hydrolase

Gene
N/A
Organism
Comamonas testosteroni (Pseudomonas testosteroni)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the meta fission degradative pathway of protocatechuate. Catalyzes the reversible hydrolysis of 2-pyrone-4,6-dicarboxylate (PDC) to a mixture of 4-carboxy-2-hydroxymuconate (CHM) and 4-oxalomesaconate (OMA).1 Publication

Catalytic activityi

2-oxo-2H-pyran-4,6-dicarboxylate + H2O = (1E)-4-oxobut-1-ene-1,2,4-tricarboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501SubstrateBy similarity
Binding sitei78 – 781SubstrateBy similarity
Binding sitei125 – 1251SubstrateBy similarity
Binding sitei131 – 1311SubstrateBy similarity
Binding sitei158 – 1581SubstrateBy similarity
Binding sitei182 – 1821SubstrateBy similarity
Active sitei258 – 2581By similarity
Binding sitei263 – 2631SubstrateBy similarity

GO - Molecular functioni

  1. 2-pyrone-4,6-dicarboxylate lactonase activity Source: UniProtKB

GO - Biological processi

  1. protocatechuate catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3463.

Names & Taxonomyi

Protein namesi
Recommended name:
2-pyrone-4,6-dicarbaxylate hydrolase
Alternative name(s):
2-pyrone-4,6-dicarboxylate lactonohydrolase
PDC hydrolase (EC:3.1.1.57)
OrganismiComamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifieri285 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeComamonas

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene completely lose the ability to grow on protocatechuate (PCA) and accumulated PDC.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3053052-pyrone-4,6-dicarbaxylate hydrolasePRO_0000422784Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ93PS7.
SMRiQ93PS7. Positions 8-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 341Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the PDC hydrolase family.Curated

Family and domain databases

InterProiIPR006992. Amidohydro_2.
[Graphical view]
PfamiPF04909. Amidohydro_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q93PS7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQFEKTPGW LDWYANPSKP QFKLPAGAVD AHCHVFGPGN EFPFAPERKY
60 70 80 90 100
TPCDASKAQL YALRDHLGFA RNVVVQATCH GADNRAMVDA CKSSGGKARG
110 120 130 140 150
VATVKRSISD AELQQLHDAG VRGVRFNFVK RLVDFTPKDE LMEIAGRIAK
160 170 180 190 200
LGWHVVIYFE AVDLPELWDF FTALPTTVVV DHMGRPDVTK GVDSEEFALF
210 220 230 240 250
LKFMREHQNV WSKVSCPERL SVTGPKALNG EQNAYRDVVP FARRVVEEFP
260 270 280 290 300
DRVLWGTDWP HPNLKDHMPD DGLLVDFIPH IAPTAELQQK LLVDNPMRLY

WPEEV
Length:305
Mass (Da):34,451
Last modified:December 1, 2001 - v1
Checksum:i2167B504424FDDDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305325 Genomic DNA. Translation: AAK73571.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305325 Genomic DNA. Translation: AAK73571.1 .

3D structure databases

ProteinModelPortali Q93PS7.
SMRi Q93PS7. Positions 8-305.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-3463.

Family and domain databases

InterProi IPR006992. Amidohydro_2.
[Graphical view ]
Pfami PF04909. Amidohydro_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate."
    Providenti M.A., Mampel J., MacSween S., Cook A.M., Wyndham R.C.
    Microbiology 147:2157-2167(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BR6020.
  2. "Characterization of the protocatechuate 4,5-cleavage pathway operon in Comamonas sp. strain E6 and discovery of a novel pathway gene."
    Kamimura N., Aoyama T., Yoshida R., Takahashi K., Kasai D., Abe T., Mase K., Katayama Y., Fukuda M., Masai E.
    Appl. Environ. Microbiol. 76:8093-8101(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPDCH_COMTE
AccessioniPrimary (citable) accession number: Q93PS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: December 1, 2001
Last modified: October 1, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3