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Q93PP9

- BFR_DESDA

UniProt

Q93PP9 - BFR_DESDA

Protein

Bacterioferritin

Gene

bfr

Organism
Desulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Iron-storage protein, whose ferroxidase center binds Fe2+ ions, oxidizes them by dioxygen to Fe3+, and participates in the subsequent Fe3+ oxide mineral core formation within the central cavity of the protein complex.

    Catalytic activityi

    4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.

    Cofactori

    Binds 1 Fe-coproporphyrin III group per dimer.1 Publication
    Binds 2 iron ions per subunit. The catalytic dinuclear iron-binding site within each subunit is known as the ferroxidase center.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi23 – 231Iron 1
    Metal bindingi56 – 561Iron 1
    Metal bindingi56 – 561Iron 2
    Metal bindingi57 – 571Iron (Fe-coproporphyrin III axial ligand); shared with dimeric partner
    Metal bindingi59 – 591Iron 1
    Metal bindingi99 – 991Iron 2
    Metal bindingi132 – 1321Iron 1
    Metal bindingi132 – 1321Iron 2
    Metal bindingi135 – 1351Iron 2

    GO - Molecular functioni

    1. ferric iron binding Source: InterPro
    2. ferroxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. intracellular sequestering of iron ion Source: UniProtKB
    2. iron ion transport Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Iron storage

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciDDES525146:GIWF-1431-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bacterioferritin (EC:1.16.3.1)
    Short name:
    BFR
    Gene namesi
    Name:bfr
    Ordered Locus Names:Ddes_1387
    OrganismiDesulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949)
    Taxonomic identifieri525146 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio
    ProteomesiUP000002598: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 179179BacterioferritinPRO_0000192591Add
    BLAST

    Interactioni

    Subunit structurei

    Homooligomer of 24 subunits, arranged as 12 dimers, that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be deposited.1 Publication

    Protein-protein interaction databases

    STRINGi525146.Ddes_1387.

    Structurei

    Secondary structure

    1
    179
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 3935
    Helixi43 – 6927
    Helixi88 – 11528
    Helixi119 – 14931
    Helixi151 – 1577

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NF4X-ray2.05A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-179[»]
    1NF6X-ray2.35A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-179[»]
    1NFVX-ray1.95A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-179[»]
    ProteinModelPortaliQ93PP9.
    SMRiQ93PP9. Positions 2-172.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ93PP9.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 150150Ferritin-like diironPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the bacterioferritin family.Curated
    Contains 1 ferritin-like diiron domain.CuratedPROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2193.
    HOGENOMiHOG000262383.
    KOiK03594.
    OrthoDBiEOG6WDSKP.

    Family and domain databases

    Gene3Di1.20.1260.10. 1 hit.
    InterProiIPR002024. Bacterioferritin.
    IPR009040. Ferritin-like_diiron.
    IPR009078. Ferritin-like_SF.
    IPR012347. Ferritin-rel.
    IPR008331. Ferritin_DPS_dom.
    [Graphical view]
    PfamiPF00210. Ferritin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002560. Bacterioferritin. 1 hit.
    PRINTSiPR00601. BACFERRITIN.
    SUPFAMiSSF47240. SSF47240. 1 hit.
    PROSITEiPS50905. FERRITIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q93PP9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGNREDRKA KVIEVLNKAR AMELHAIHQY MNQHYSLDDM DYGELAANMK    50
    LIAIDEMRHA ENFAERIKEL GGEPTTQKEG KVVTGQAVPV IYESDADQED 100
    ATIEAYSQFL KVCKEQGDIV TARLFERIIE EEQAHLTYYE NIGSHIKNLG 150
    DTYLAKIAGT PSSTGTASKG FVTATPAAE 179
    Length:179
    Mass (Da):19,881
    Last modified:December 1, 2001 - v1
    Checksum:i09E3387462FED3A3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF321851 Genomic DNA. Translation: AAK60120.1.
    CP001358 Genomic DNA. Translation: ACL49289.1.
    RefSeqiWP_012625013.1. NC_011883.1.
    YP_002479967.1. NC_011883.1.

    Genome annotation databases

    EnsemblBacteriaiACL49289; ACL49289; Ddes_1387.
    GeneIDi7285082.
    KEGGidds:Ddes_1387.
    PATRICi21736512. VBIDesDes50650_1607.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF321851 Genomic DNA. Translation: AAK60120.1 .
    CP001358 Genomic DNA. Translation: ACL49289.1 .
    RefSeqi WP_012625013.1. NC_011883.1.
    YP_002479967.1. NC_011883.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1NF4 X-ray 2.05 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P 1-179 [» ]
    1NF6 X-ray 2.35 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P 1-179 [» ]
    1NFV X-ray 1.95 A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P 1-179 [» ]
    ProteinModelPortali Q93PP9.
    SMRi Q93PP9. Positions 2-172.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 525146.Ddes_1387.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACL49289 ; ACL49289 ; Ddes_1387 .
    GeneIDi 7285082.
    KEGGi dds:Ddes_1387.
    PATRICi 21736512. VBIDesDes50650_1607.

    Phylogenomic databases

    eggNOGi COG2193.
    HOGENOMi HOG000262383.
    KOi K03594.
    OrthoDBi EOG6WDSKP.

    Enzyme and pathway databases

    BioCyci DDES525146:GIWF-1431-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q93PP9.

    Family and domain databases

    Gene3Di 1.20.1260.10. 1 hit.
    InterProi IPR002024. Bacterioferritin.
    IPR009040. Ferritin-like_diiron.
    IPR009078. Ferritin-like_SF.
    IPR012347. Ferritin-rel.
    IPR008331. Ferritin_DPS_dom.
    [Graphical view ]
    Pfami PF00210. Ferritin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002560. Bacterioferritin. 1 hit.
    PRINTSi PR00601. BACFERRITIN.
    SUPFAMi SSF47240. SSF47240. 1 hit.
    PROSITEi PS50905. FERRITIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genetic organization of Desulfovibrio desulphuricans ATCC 27774 bacterioferritin and rubredoxin-2 genes: involvement of rubredoxin in iron metabolism."
      da Costa P.N., Romao C.V., LeGall J., Xavier A.V., Melo E., Teixeira M., Saraiva L.M.
      Mol. Microbiol. 41:217-227(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete sequence of Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774."
      US DOE Joint Genome Institute
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Lu M., Kiss H., Meineke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L.
      , Kyrpides N., Ovchinnikova G., Hazen T.C.
      Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27774 / DSM 6949.
    3. "A bacterioferritin from the strict anaerobe Desulfovibrio desulfuricans ATCC 27774."
      Romao C.V., Regalla M., Xavier A.V., Teixeira M., Liu M.-Y., Le Gall J.
      Biochemistry 39:6841-6849(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, SUBUNIT, REDOX POTENTIOMETRY OF HEME, ABSORPTION SPECTROSCOPY, EPR SPECTROSCOPY.
    4. "Iron-coproporphyrin III is a natural cofactor in bacterioferritin from the anaerobic bacterium Desulfovibrio desulfuricans."
      Romao C.V., Louro R., Timkovich R., Lubben M., Liu M.Y., LeGall J., Xavier A.V., Teixeira M.
      FEBS Lett. 480:213-216(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: COFACTOR.
    5. "Structure determination of bacterioferritin from Desulfovibrio desulfuricans by the MAD method at the Fe K-edge."
      Coelho A.V., Macedo S., Matias P.M., Thompson A.W., LeGall J., Carrondo M.A.
      Acta Crystallogr. D 57:326-329(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CRYSTALLIZATION.
    6. "The nature of the di-iron site in the bacterioferritin from Desulfovibrio desulfuricans."
      Macedo S., Romao C.V., Mitchell E., Matias P.M., Liu M.Y., Xavier A.V., LeGall J., Teixeira M., Lindley P., Carrondo M.A.
      Nat. Struct. Biol. 10:285-290(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).

    Entry informationi

    Entry nameiBFR_DESDA
    AccessioniPrimary (citable) accession number: Q93PP9
    Secondary accession number(s): B8J0L2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 23, 2003
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3