Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Urease subunit beta

Gene

ureB

Organism
Helicobacter hepaticus (strain ATCC 51449 / 3B1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit alpha (ureA), Urease subunit beta (ureB)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi136 – 1361Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi138 – 1381Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi219 – 2191Nickel 1; via carbamate groupUniRule annotation
Metal bindingi219 – 2191Nickel 2; via carbamate groupUniRule annotation
Binding sitei221 – 2211SubstrateUniRule annotation
Metal bindingi248 – 2481Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi274 – 2741Nickel 2; via tele nitrogenUniRule annotation
Active sitei322 – 3221Proton donorUniRule annotation
Metal bindingi362 – 3621Nickel 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciHHEP235279:GHUA-424-MONOMER.
BRENDAi3.5.1.5. 7587.
UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit betaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit betaUniRule annotation
Gene namesi
Name:ureBUniRule annotation
Ordered Locus Names:HH_0408
OrganismiHelicobacter hepaticus (strain ATCC 51449 / 3B1)
Taxonomic identifieri235279 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000002495 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 569569Urease subunit betaPRO_0000234158Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei219 – 2191N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Expressioni

Inductioni

By nickel ions.1 Publication

Interactioni

Subunit structurei

Heterohexamer of 3 UreA (alpha) and 3 UreB (beta) subunits.UniRule annotation

Protein-protein interaction databases

STRINGi235279.HH0408.

Structurei

3D structure databases

ProteinModelPortaliQ93PJ4.
SMRiQ93PJ4. Positions 1-569.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini131 – 569439UreaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiEOG6ND0GM.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q93PJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKISRKQYA SMYGPTTGDK VRLGDTNLFA EIEKDYTLYG EEIKFGGGKT
60 70 80 90 100
IRDGMAQSAS TYTNELDAVI TNAMIIDYTG IYKADIGIKG GKIVGIGKAG
110 120 130 140 150
NPDTQDSVNE AMVVGAATEV IAGEGQIITA GGIDTHIHFI SPTQIPTALY
160 170 180 190 200
SGVTTMIGGG TGPAAGTNAT TCTPGKWNMH QMLRAAESYA MNLGFFGKGN
210 220 230 240 250
SSNEEGLEEQ IKAGALGLKV HEDWGSTPAA INHALNVAQK YDVQVAIHTD
260 270 280 290 300
TLNEAGCVED TMKAIDGRTI HTFHTEGAGG GHAPDIIKAA GEPNILPAST
310 320 330 340 350
NPTIPFTKNT ADEHLDMLMV CHHLDKKIKE DVAFADSRIR PETIAAEDTL
360 370 380 390 400
HDMGIFSITS SDSQAMGRVG EVITRTWQTA DKCKNEFGAL KEECGENDNF
410 420 430 440 450
RIKRYISKYT INPAIAHGIS EYVGSVEVGK FADLVLWKPS MFGIKPEMIL
460 470 480 490 500
KNGMIVAAKI GDSNASIPTP EPVVYAPMFG SYGKAKYNCA ITFVSKIAYD
510 520 530 540 550
CHIKEELGLE RILLPVKNCR NITKKDMKFN DVITPIEVNP ETYEVRVNNT
560
KITSKPVEKV SLGQLYCLF
Length:569
Mass (Da):61,510
Last modified:December 1, 2001 - v1
Checksum:i2825D1E6406F6429
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332656 Genomic DNA. Translation: AAK69199.1.
AE017125 Genomic DNA. Translation: AAP77005.1.
RefSeqiWP_011115250.1. NC_004917.1.

Genome annotation databases

EnsemblBacteriaiAAP77005; AAP77005; HH_0408.
KEGGihhe:HH_0408.
PATRICi20588268. VBIHelHep90276_0413.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332656 Genomic DNA. Translation: AAK69199.1.
AE017125 Genomic DNA. Translation: AAP77005.1.
RefSeqiWP_011115250.1. NC_004917.1.

3D structure databases

ProteinModelPortaliQ93PJ4.
SMRiQ93PJ4. Positions 1-569.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi235279.HH0408.

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP77005; AAP77005; HH_0408.
KEGGihhe:HH_0408.
PATRICi20588268. VBIHelHep90276_0413.

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiEOG6ND0GM.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.
BioCyciHHEP235279:GHUA-424-MONOMER.
BRENDAi3.5.1.5. 7587.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression, and catalytic activity of Helicobacter hepaticus urease."
    Beckwith C.S., McGee D.J., Mobley H.L.T., Riley L.K.
    Infect. Immun. 69:5914-5920(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MU94-1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51449 / 3B1.
  3. "Differential regulation of urease activity in Helicobacter hepaticus and Helicobacter pylori."
    Belzer C., Stoof J., Beckwith C.S., Kuipers E.J., Kusters J.G., van Vliet A.H.M.
    Microbiology 151:3989-3995(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiURE1_HELHP
AccessioniPrimary (citable) accession number: Q93PJ4
Secondary accession number(s): Q7BYA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

The orthologous protein is known as the alpha subunit (UreC) in most other bacteria.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.