Q93NX8 (Q93NX8_9ACTO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
AmphJ EMBL AAK73502.1

Gene names

Name:

amphJ EMBL AAK73502.1

Organism

Streptomyces nodosus EMBL AAK73502.1

Taxonomic identifier

40318 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	5644 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Keywords
Ligand	Phosphopantetheine RuleBase RU000721
Molecular function	Transferase RuleBase RU000693
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: InterPro cofactor binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q93NX8 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: CF6A35E3BA0D2658	FASTA	5,644	585,795
	10 20 30 40 50 60 MEQTMNAPAE NVVAALRAAV KETERLRRRN RTIVAAAREP IAVVGMGCRF PGGVDSPQAL 70 80 90 100 110 120 WEMVAGGTDV ISEFPDDRGW DLEALRTSGI DDRDTSVSQR GGFLDSIADF DPGFFGISPR 130 140 150 160 170 180 EAVTMDPQQR LLLETAWEAI ERARIDATRL RGTRTGTFIG TNGQDYAYLL VRSLDDATGD 190 200 210 220 230 240 VGTGIAASAV SGRLSYTFGL EGPAITVDTA CSSSLVALHL AVQSLRNGEC TLALAGGVNV 250 260 270 280 290 300 MSTPGSLVEF SRQGGLAGDG RCKAFSDSAD GTGWSEGAAV LALERLSDAQ RNGHPVLAVI 310 320 330 340 350 360 RGSAVNQDGA SNGFTAPNGP SQQRVIRQAL SNAGLNPADV DVVEAHGTGT PLGDPIEAQS 370 380 390 400 410 420 ILATYGQDRE QPLLLGSIKS NIGHTQSAAS GVAGIMKMIM AMRNEVLPKT LHVDRPSTHV 430 440 450 460 470 480 DWTAGKVELL TENRPWPTAP DRPRRSGVSS FGVSGTNAHV IVEQAPQTPA HQPEEAPADP 490 500 510 520 530 540 SDEAPAAPRT AGVLPWVLSA RSAAALREQA AALLAHLDAP GAPGALDTGY SLATTRASLE 550 560 570 580 590 600 HRLAVVTGAD GTAGREALTS WLAGDPAPDA HEGRPVGRTR SAFLFSGQGA QRLGMGRELH 610 620 630 640 650 660 ARFPVFAEAL DQVLDLLDEE LDASLGDIIW GEEEAPLNET GFTQPALFAV EVALYRLVES 670 680 690 700 710 720 WGVTPDFVAG HSIGEIAAAH VAGVFSLEDA CRLVAARAGL MQALPSGGAM VAVEATEDEV 730 740 750 760 770 780 LPLLTEGVAV AAVNGPTSVV VSGEEKATLA VAEQLAAKGR RTSRLRVSHA FHSPLMDPML 790 800 810 820 830 840 DDFRAVAETL SYDEPQLPVV SNLTGTLAAD GQLTSPEYWV RHVREAVRFA DGVRALAEAG 850 860 870 880 890 900 ADVLLELGPD GVLAALAQQS ATALTVPFLR KDRPEEHSAV TALARLHTAG VTVDWAAFHD 910 920 930 940 950 960 GTGARTGELP TYAFQHERYW PKATATAVDA TGLGLASADH PLLGAAMSVA GSDELLLTGT 970 980 990 1000 1010 1020 LSLATHPWLA DHSVDGMVVF PGTGFLELAV RAADQAGCDR VQELTVATPL VLPATGAVQM 1030 1040 1050 1060 1070 1080 QISVGAAGED GSRELRFFTR PGEDFDAEWT QHATGRIASG EHVLGFDTTV WPPRDSEAVD 1090 1100 1110 1120 1130 1140 IEELFDRFAS DGLEYGPVFR GLRAAWRQDD TVYAEVELPD SVEDAGAFGL HPALLHAALH 1150 1160 1170 1180 1190 1200 GTAFLSDDSG LLPFAWEGVS LHADGASTLR VRIASCGEDT VEIAAVDPAG QPVLSVESLT 1210 1220 1230 1240 1250 1260 LRAADSGAGA ASRREEANSL FRVDWTPRTV AAPAAPATWA VLGEDPFGLT AALAGDSEAV 1270 1280 1290 1300 1310 1320 AGVHAPAATL EELAARSGAV PDMVAVTVRG DADGGPEAAR ELTREVLALV QGWLAEPAFA 1330 1340 1350 1360 1370 1380 SSRLVVVTRD AVADGERGAV DLAAAPVWGL VRSAQSENPG RLLLADVDDT ADSLARLPLL 1390 1400 1410 1420 1430 1440 AGLFDAEEPQ AVVREGTVRV GRLARLESGD SSARALDPEG TVLVTGGTGG LASALARHLV 1450 1460 1470 1480 1490 1500 AEHDIRHLLL TSRRGPDAPG AADLVQALAE LGAEARVAAC DVADREALAA LLASVPAEHP 1510 1520 1530 1540 1550 1560 LTAVVHTAGV LDDGIFPSLT PDRLDSVMRP KVDAAWHLHD LTRDLDLAAF VLYSSTAGVM 1570 1580 1590 1600 1610 1620 GSPGQANYAA GNTFLDALAA HRQSLGLPAT SLAWGAWEQG VGMTGQLSGQ DARRISDAGG 1630 1640 1650 1660 1670 1680 MPLLSVERGL ALYDAAMLAD EPLVVPLGLG GGGPLPAGAG VPAILRGLVR TGGRRARAAT 1690 1700 1710 1720 1730 1740 AAVARAGLAE RLAVLPEEQR RPFVVDLVRA EAAAVLGHGS ADAVDSRREF RGLGFDSLTA 1750 1760 1770 1780 1790 1800 IELRNRLGKA TGLTLPATLV FDYPTPEQLA DHLLDELLGA DAIEVFAASQ SAADVHDDPV 1810 1820 1830 1840 1850 1860 VIVGMGCRFP GGVGSPEDLW DLLASGSDAI TGFPADREWE SSRLVAGEAG GVSAQGGFLS 1870 1880 1890 1900 1910 1920 DIAGFDADFF GISPREALAM DPQQRILLEV TWEAIERAGV DPTALRGSRT GVFMGVNGQD 1930 1940 1950 1960 1970 1980 YSSLVMGSRD DVAGHATAGL AVSVVSGRLS YALGLEGPAL SVDTACSSSL VSLHLAAQAL 1990 2000 2010 2020 2030 2040 RSGECSMALV GGVTVMTTPA NFAGFSRMGG LAQDGRCKAF SDSADGTGWS EGAAVLVVER 2050 2060 2070 2080 2090 2100 LSDARRAGHR VLAVVRGSAV NQDGASNGLT APNGPSQQRV IRQALANAGL RPGDVDAVEA 2110 2120 2130 2140 2150 2160 HGTGTPPGDP IEAQALLATY GSDRDPQQPL LLGSVKSNIG HTQAAAGVAG LVKMVMAMRN 2170 2180 2190 2200 2210 2220 GVLPRTLHIT EPSTHVDWSL GAVQVLTEET AWPETGRVRR AGVSSFGISG TNAHVILEGA 2230 2240 2250 2260 2270 2280 PDEPVPAPVA DRPVPGAVAW PVSAKSEGAL DDQAERLRES ADALPALDTA YTLATGRADF 2290 2300 2310 2320 2330 2340 EHRAVLLAAD GTLTEVARGV AEPHRSAFLF SGQGAQRLGM GRELHARFPV FAEAFDSVTA 2350 2360 2370 2380 2390 2400 LLESELDTSV REVMWGTDEG ALNATAFTQP ALFAVEVALY RLVESWGVTP DFVAGHSVGE 2410 2420 2430 2440 2450 2460 IAAAHVAGVF SLEDACRLVA ARAGLMQALP SGGAMVAVEA TEDEVLPLLT EGVAVAAVNG 2470 2480 2490 2500 2510 2520 PTSVVVSGEE QATLAVAEQL AAQGRRTSRL RVSHAFHSPL MDPMLEDFRA VAETLSYHEP 2530 2540 2550 2560 2570 2580 RIPVVSNLTG EVAAAGVHTH PDYWVRHVRE AVRFADGVRG LADRGVTALL EIGPDGVLSA 2590 2600 2610 2620 2630 2640 LAAASLTDTD TVVVPALRKD RDETVSVLSG VARLYVAGVD VDWSAPLSGA GARIADVPTY 2650 2660 2670 2680 2690 2700 AFQHERYWPK AAPAALDATG LGLASADHPL LGAAMSVAGS DELLLTGSLS AATHPWLADH 2710 2720 2730 2740 2750 2760 VVGGMIFFPG TGFLELAVRA ADQAGCDRVE ELMIAAPLVL PATGAVQVQI SVGAADEEGS 2770 2780 2790 2800 2810 2820 RELRFFTRPG EDFDAEWTQH ATGRIGSGEQ VIDFDATVWP PRDAEAIDID GMFERYAADG 2830 2840 2850 2860 2870 2880 LEYGPVFRGL RAVWRQDDTV YAEVALPESV EDADAFGLHP ALFDAALHST VFLSAEGDTR 2890 2900 2910 2920 2930 2940 SLLPFAWEGV SLHADGASTL RVRIASCGED TVQIAAVDPG GQPVVSVESL TLRAAGPGDA 2950 2960 2970 2980 2990 3000 AEPRRDDSNS LLRVDWTART LGAPAAPATW AVLGEDPFGL TAALAGDSEA VAGVHAPAAT 3010 3020 3030 3040 3050 3060 IEELAARSGA VPDMVAVTVR GDADAGPDDA HELAHEVLAL VQGWLAEPAF ASSRLVVVTR 3070 3080 3090 3100 3110 3120 NAVADGERGA VDLAAAPVWG LVRSAQSENP GRLLLADVDD TADSLARLPL LAGLFDAEEP 3130 3140 3150 3160 3170 3180 QAVVREGTVR VGRLARLESG TSLVPPAGTP WRLGCRAKGS LDGLALLPYP EAVTPLTGRE 3190 3200 3210 3220 3230 3240 VRIGIRAAGL NFRDVLNALG MYPGEAGLFG SEGAGVVSEV GPDVTGLAPG DRVMGMVFGG 3250 3260 3270 3280 3290 3300 FGPLGVADER LLTRVPDDWS WETASSVPLV FLTAYHALKD LAGLRPGEKI LIHAGAGGVG 3310 3320 3330 3340 3350 3360 MAAIQIAHHL GAEVFATASE GKWDVLRSLG VADDHIASSR TLDFETAFTE VAGDKGLDVV 3370 3380 3390 3400 3410 3420 LNALAGEFVD ASMRLLGTGG RFLEMGKTDI RDSDAASDGI TYRFFDLGMV DPDHIQQMLL 3430 3440 3450 3460 3470 3480 DLVDLFERDV LSPLPVRAWD VRRSREAFRF MSMAKHIGKI VLTMPRAMDP EGTVLVTGGT 3490 3500 3510 3520 3530 3540 GGLASALARH LVAEHGIKHL LLTSRRGPDA PGAADLVQAL AELGAEARVA ACDVADRDAL 3550 3560 3570 3580 3590 3600 AGLLASVPAE RPLTAVVHTA GVLDDGILAS LTPDRLDTVM RPKVDAAWHL HDLTRDLDLA 3610 3620 3630 3640 3650 3660 AFVLYSSTSG VFGSPGQANY AAGNTFLDAL AAHRQSLGLP ATSLAWNAWE QGSGMTSGLS 3670 3680 3690 3700 3710 3720 DEDMRRINDN SGMPLLSVER GLALYDAATL ADEPLVVPLG LGGGGSLPPG MSVPAILRGL 3730 3740 3750 3760 3770 3780 VRTGGRRAKA GAAAVARAGL AERLAVLPEE QRLPFVVDLV RAEAATVLGH GSADAVDARR 3790 3800 3810 3820 3830 3840 EFRGLGFDSL TAIELRNRLG KASGLTLTAT LVFDYPTPQQ LAEHLLDELL GADAAEAFAA 3850 3860 3870 3880 3890 3900 PQTAAAASDD DPVVIVGMGC RFPGGVGSPE ELWDLVASGT DAITGFPADR EWESSTIGGE 3910 3920 3930 3940 3950 3960 PGDLSGQGGF LSDIADFDAD FFGIAPREAL AMDPQQRILL EVTWEAVERA GLDPTALRGS 3970 3980 3990 4000 4010 4020 RTGVFMGVSG QDYSGLVMRS RDDIASHATT GLAVSVVSGR LSYTLGLEGP ALSVDTACSS 4030 4040 4050 4060 4070 4080 SLVSLHLAAQ ALRSGECTMA LAGGVTVMTT PANFTGFSKM GGLAHDGRCK AFSDSADGTG 4090 4100 4110 4120 4130 4140 WSEGAAVLVL ERLSDARRAG HRVLAVVRGS AVNQDGASNG LTAPNGPSQQ RVIRQALANA 4150 4160 4170 4180 4190 4200 GLRPGDVDAV EAHGTGTPLG DPIEAQALIA TYGSDRDPQQ PLLLGSVKSN IGHTQSAAGA 4210 4220 4230 4240 4250 4260 AGLVKMVMAM HQGTLPRTLH VTEPSTHVDW SLGAVRLLTE ETAWPETGRV RRAGVSSFGI 4270 4280 4290 4300 4310 4320 SGTNAHVILE GAPEPTADDR SAEDTVTPAV TPWVVSARSE QALDAQLERL RAHAAAHPEL 4330 4340 4350 4360 4370 4380 SGADIGLSLV TSRPSFEHRA VLLAGPDGIT EAARAEAGTA RTPAFLFSGQ GAQRLGMGRE 4390 4400 4410 4420 4430 4440 LHARFPVFAE VFDSVTALLE SELGTSVREV MWGTDEAALN STAFTQPALF AVEVALYRLV 4450 4460 4470 4480 4490 4500 ESWGVTPDFV AGHSVGEIAA AHVAGVFSLE DACRLVAARA RLMDALPRGG AMAAVEATED 4510 4520 4530 4540 4550 4560 EVLPLLDDGV AVAAVNGPTS VVVSGPEDGV DQLVALLESD GRRTTRLRVS HAFHSSLMDP 4570 4580 4590 4600 4610 4620 MLEDFRAVAE TLSYHEPRIP VVSNLTGEVA SAGTHTHPDY WVRHVREAVR FADGVRALAD 4630 4640 4650 4660 4670 4680 RGVTAFLEIG PDGVLSALAA ASLPDTGTVV VPALRKDRDE TVSVLTALAR LHTAGLDTDW 4690 4700 4710 4720 4730 4740 SAHFAGTGAR TVELPTYAFQ GTRFWPDTTA APGDAGGLGL DAGGHPLLTA ATSVAGSDET 4750 4760 4770 4780 4790 4800 LLSGRLSAAA QPWLTGRTEN GTTILPTAVL AELALHAAEA CDRTTVENLT VGAPLALTGN 4810 4820 4830 4840 4850 4860 RPQRLQVLVG VPDETGRRTL TVHTRADGDD APWVERATAM LTDAPAAATP DTVWPPADAT 4870 4880 4890 4900 4910 4920 PVDELPEPTG PSVLRAAWRR GGDVFAEVEI TEQSPAEQAF ALHPALLDTA VRAAVLLEGD 4930 4940 4950 4960 4970 4980 GGGSGDDTLD AVAWDGLVLH AAHPVLLRVR LTATGDDTWA LEATDPQGGP VLSVASVTLG 4990 5000 5010 5020 5030 5040 ATVAAPVTGA PATDDAALLA LDWVAPAPAP RSGDSGPWTV LGDALPGLDT ALAAVDNVLV 5050 5060 5070 5080 5090 5100 TRADSLAELL DSGAPLPSLM LLPVEGGPTA GHDLPAAVRA ATTRVLDLLR RWTSDPRTAD 5110 5120 5130 5140 5150 5160 SRLAIVTRGA VAAGREDVTD LAAAAVWGLV RSAQSENPGC FLLLDLDPAD AAEATDAAVL 5170 5180 5190 5200 5210 5220 ASLPALFDAG ETQAAVRGGA LTVARLTRTE ATPAPAADQV RAWDRDGTVL ITGGTGGLGA 5230 5240 5250 5260 5270 5280 VLARHLVTGH GIKHLLLAGR RGPDAPGATA LSKELSALGA EVTVRACDVS DRSAVDALLA 5290 5300 5310 5320 5330 5340 GLPAEHPLTA VVHTAGVLDD ATIGTLTAEQ LDTVLRPKAD AAWHLHQATR ALPLAGFVLY 5350 5360 5370 5380 5390 5400 SSVAGVTGGP GQGNYAAANT FLDALAAHRA AQGLPALSLA WGPWGQGAGM TGTLSDADLE 5410 5420 5430 5440 5450 5460 RMERSGMPPL TERQGLALFD AANGHDEALA VAIRVSRSAA APDAGEVPAV LRSLVRARRR 5470 5480 5490 5500 5510 5520 AAATAGADGL TRRLAGLGAE QRHETLVGLV RQETAGVLGH SGADAVPADR DFSRLGFDSL 5530 5540 5550 5560 5570 5580 MAVELRTRLS AATGVRLPST LVFDHPTPAA VARHLADSLT GQDRSGTAAS PLAALDRLEA 5590 5600 5610 5620 5630 5640 ELSADGVDEA VRRGVEGRLR RLLAAWDGTG SDGNGPAVEE RIEAASAEEI FAFIDNELGR SSDS

References

[1]	"Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes." Caffrey P., Lynch S., Flood E., Finnan S., Oliynyk M. Chem. Biol. 8:713-723(2001) [PubMed: 11451671] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Biosynthesis of amphotericin derivatives lacking exocyclic carboxyl groups." Carmody M., Murphy B., Byrne B., Power P., Rai D., Rawlings B., Caffrey P. J. Biol. Chem. 280:34420-34426(2005) [PubMed: 16079135] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF357202 Genomic DNA. Translation: AAK73502.1.
3D structure databases
HSSP	HSSP built from PDB template 1PQW based on UniProtKB P96202.
ProteinModelPortal	Q93NX8.
SMR	Q93NX8. Positions 5466-5559.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001227. Ac_transferase_dom. IPR009081. Acyl_carrier_prot-like. IPR014043. Acyl_transferase. IPR016035. Acyl_Trfase/lysoPLipase. IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR000794. Beta-ketoacyl_synthase. IPR002198. DH_sc/Rdtase_SDR. IPR011032. GroES-like. IPR018201. Ketoacyl_synth_AS. IPR014031. Ketoacyl_synth_C. IPR014030. Ketoacyl_synth_N. IPR016036. Malonyl_transacylase_ACP-bd. IPR016040. NAD(P)-bd_dom. IPR006163. Phsphopanteth-bd. IPR020842. PKS/FAS_KR. IPR020801. PKS_acyl_transferase. IPR020841. PKS_Beta-ketoAc_synthase_dom. IPR020807. PKS_dehydratase. IPR020843. PKS_ER. IPR013968. PKS_KR. IPR020806. PKS_PP-bd. IPR006162. PPantetheine_attach_site. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.366.10. Ac_transferase_reg. 6 hits. G3DSA:1.10.1200.10. ACP_like. 3 hits. G3DSA:3.40.50.720. NAD(P)-bd. 4 hits. G3DSA:3.40.47.10. Thiolase-like_subgr. 6 hits.
PANTHER	PTHR11712. Ketoacyl_synth. 1 hit.
Pfam	PF00698. Acyl_transf_1. 3 hits. PF08240. ADH_N. 1 hit. PF00106. adh_short. 1 hit. PF00107. ADH_zinc_N. 1 hit. PF00109. ketoacyl-synt. 3 hits. PF02801. Ketoacyl-synt_C. 3 hits. PF08659. KR. 2 hits. PF00550. PP-binding. 3 hits. [Graphical view]
SMART	SM00827. PKS_AT. 3 hits. SM00826. PKS_DH. 3 hits. SM00829. PKS_ER. 1 hit. SM00822. PKS_KR. 3 hits. SM00825. PKS_KS. 3 hits. SM00823. PKS_PP. 3 hits. [Graphical view]
SUPFAM	SSF47336. ACP_like. 3 hits. SSF52151. Acyl_Trfase/lysoPlipase. 3 hits. SSF50129. GroES_like. 1 hit. SSF55048. Malonyl_transacylase_ACP-bd. 3 hits. SSF53901. Thiolase-like. 3 hits.
PROSITE	PS50075. ACP_DOMAIN. 3 hits. PS00606. B_KETOACYL_SYNTHASE. 3 hits. PS00012. PHOSPHOPANTETHEINE. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q93NX8_9ACTO

Accession

Primary (citable) accession number: Q93NX8

Entry history

Integrated into UniProtKB/TrEMBL:	December 1, 2001
Last sequence update:	December 1, 2001
Last modified:	December 14, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)