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Q93NW7

- Q93NW7_9ACTO

UniProt

Q93NW7 - Q93NW7_9ACTO

Protein
Submitted name:

AmphB

Gene

amphB

Organism
Streptomyces nodosus
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei2858 – 28581NADPImported
    Binding sitei2895 – 28951NADPImported
    Binding sitei2924 – 29241NADP; via amide nitrogenImported
    Binding sitei2929 – 29291NADP; via amide nitrogenImported

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi2756 – 27583NADPImported
    Nucleotide bindingi2779 – 27802NADPImported
    Nucleotide bindingi2807 – 28082NADPImported
    Nucleotide bindingi2833 – 28353NADPImported

    GO - Molecular functioni

    1. cofactor binding Source: InterPro
    2. nucleotide binding Source: UniProtKB-KW
    3. oxidoreductase activity Source: InterPro
    4. phosphopantetheine binding Source: InterPro
    5. transferase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    TransferaseUniRule annotation

    Keywords - Ligandi

    NADPImported, Nucleotide-bindingImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    AmphBImported
    Gene namesi
    Name:amphBImported
    OrganismiStreptomyces nodosusImported
    Taxonomic identifieri40318 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    PTM / Processingi

    Keywords - PTMi

    PhosphopantetheineUniRule annotation

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-59008N.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3MJCX-ray1.48A/B2529-3003[»]
    3MJEX-ray1.36A/B2529-3003[»]
    3MJSX-ray1.40A/B2529-3003[»]
    3MJTX-ray1.60A/B2529-3003[»]
    3MJVX-ray1.46A/B2529-3003[»]
    4DIFX-ray1.52A/B2529-3003[»]
    ProteinModelPortaliQ93NW7.
    SMRiQ93NW7. Positions 9-922, 1462-1552, 3015-3105.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ93NW7.

    Family & Domainsi

    Family and domain databases

    Gene3Di1.10.1200.10. 2 hits.
    3.40.366.10. 4 hits.
    3.40.47.10. 4 hits.
    3.40.50.720. 2 hits.
    InterProiIPR001227. Ac_transferase_dom.
    IPR009081. Acyl_carrier_prot-like.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR002198. DH_sc/Rdtase_SDR.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016036. Malonyl_transacylase_ACP-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR020842. PKS/FAS_KR.
    IPR020801. PKS_acyl_transferase.
    IPR020841. PKS_Beta-ketoAc_synthase_dom.
    IPR013968. PKS_KR.
    IPR020806. PKS_PP-bd.
    IPR015083. Polyketide_synth_docking.
    IPR006162. PPantetheine_attach_site.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF00698. Acyl_transf_1. 2 hits.
    PF00106. adh_short. 1 hit.
    PF08990. Docking. 1 hit.
    PF00109. ketoacyl-synt. 2 hits.
    PF02801. Ketoacyl-synt_C. 2 hits.
    PF08659. KR. 1 hit.
    PF00550. PP-binding. 2 hits.
    [Graphical view]
    SMARTiSM00827. PKS_AT. 2 hits.
    SM00822. PKS_KR. 2 hits.
    SM00825. PKS_KS. 2 hits.
    SM00823. PKS_PP. 2 hits.
    [Graphical view]
    SUPFAMiSSF47336. SSF47336. 2 hits.
    SSF52151. SSF52151. 4 hits.
    SSF53901. SSF53901. 2 hits.
    SSF55048. SSF55048. 2 hits.
    PROSITEiPS50075. ACP_DOMAIN. 2 hits.
    PS00606. B_KETOACYL_SYNTHASE. 2 hits.
    PS00012. PHOSPHOPANTETHEINE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q93NW7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPEPQQNQQE KVVDYLRRVT NDLRRARRRI GELESRDNEP VAIVGMSCRL     50
    PGGVSSPESL WELVESGGDA ISGFPADRGW DIEALTGKGE GSSSTHEGGF 100
    LYEATDFDAG FFGISPREAT AMDPQQRLLL EVSWEALERA GIAPTSLRSS 150
    ATGVFVGSYH WGAPSADTAT ELHGHALTGT AASVLSGRLS YVYGLEGPAV 200
    TVDTACSSSL VALHMAAQSL RNGESSLALV GGVTILAEPS VFVEFSAQGG 250
    LAQDGRCKAF SDSADGTGWS EGAAVLVVER LSDARRAGHR VLAVVRGSAV 300
    NQDGASNGLT APNGPSQQRV IRQALANAGL RPGDVDAVEA HGTGTPLGDP 350
    IEAQALIATY GSDRDPQQPL LLGSVKSNIG HTQSAAGVAG LVKMVMAMHN 400
    GTLPRTLHLT EPSTHVDWSL GAVRLLTEET AWPETGRVRR AAVSSFGISG 450
    TNAHAVLEQA PVPAEDAEDA GTEDAPQQPA TVPGALPWLL SAHSGQALCD 500
    QAAALVTRLD ADPEPRPQDI GQGLLTSRAL LERRAVVVGT GRDELLTGSR 550
    ALAEGQSWNG LAEGTADVDG RTVFVFPGQG SQWVGMGARL LDESPVFAER 600
    IAECAAALAE FTDWDLLDVL RGAEGAPTLE RVDVVQPASF AVMVSLAAVW 650
    RAQGVEPDAV VGHSQGEIAA AVVSGALSLR DGARVVALRS QAIGRTLAGR 700
    GGMMSVALPV ADVEARLEAF GGRVSVAAEN GPRSCVVSGE PGALDELSEL 750
    LTAEGVRVRR VPVDYASHSH HVEDLHDEIL QLLAEVAPKA SEVPLFSTVT 800
    GDWLDTTVMD AGYWFRSLRG RVLFADAIRG LLDTEHRAFI EVSSHPVLAM 850
    SVQDMIDDAG VVGVASGTLR RDNGGLDRFL LSAAQVFVRG VQVDWAPLFE 900
    GTGATRVDLP TYAFQHERYW NDRAVVDRSA SAPMDAEFWA AVEQADVSAL 950
    TEALGTDEES VAAILPGLAS WRRTRGERST VDSWRYRVVW EPLAQIPRAT 1000
    LDGTWLLVSA GGVDDDVAEV LEAGGAEVRR LVLDETCTDP AVLREHLTDT 1050
    DGLTGIVSVL ADAEEGCVRH PGLTLGLALS VSLAQALGEA DVTAPLWFLT 1100
    CGAFSTGPSD TVTRPLQSQI AGLGWTVAVE HPHRWGGGVD LPEALDARAA 1150
    QRLVAALAGA LGDDDQLAVR PAGVLARRIV RASGDTRRKA RSWKPRGTTL 1200
    VTGGSGTLAP GLARHLAAQG AEHLVLLSRR GADAPGAAEL AAELQAAGTE 1250
    VRFAACDITD PDAVAALLAD LKAEGRTVRT VVHTAAVIEL AALADTTVDA 1300
    FADVVHAKVT GARILDELLD DEELDDFVLY SSTAGMWGSG VHAAYVAGNA 1350
    YLSALAEQRR ARGARATSIH WGKWPDDLER ELADPHQIRR SGLEYLDPEL 1400
    AMTALTRVME DDETVIGLMD IDWGTYHDVF TAGRPSHLFD RIPEVARLLA 1450
    DRAAPAATAV ATSGLAARLQ GVSAAEQDRI VLSVVREETA AVLGHASAET 1500
    VPERRAFRDI GFDSVTAVDL RNRLVAATGL TLPSTMVFDH PNAVALATFL 1550
    KATALGTTGT AGDRPTAAVT AGADDDLIVI VGMSCRFPGG ANTPEELLRL 1600
    ALDGADVISE FPADRGWDAH GLYDPDPDRQ GRTYSVHGGF LHEAAGFDAG 1650
    FFGISPREAL AMDPQQRLLL ETSWEVLERA GIDPESLHST ATGAFFGASY 1700
    QDYSSSVQNG TEGAEVHMMT GTAASVLSGR ISYLLGLEGP AVTVDTACSS 1750
    SLVAMHLACQ SLRSGESSLA LAGGAAVMAT PHAFIGFSRQ RALAENGRCK 1800
    PFAEGADGMT LAEGVGVVLL EKLSDARRNG HRVLAVVRGS ALNQDGASNG 1850
    LTAPNGLSQQ RVIRQALANA GLGTADIDVV EAHGTGTKLG DPIEAGALLA 1900
    TYGQGRDGGQ PLLLGSVKSN IGHTQAAAGV AGVIKMVMAM HAGELPGTLH 1950
    IDEPSSHVDW TSGAVTLLRE RTEWPAVGDR PRRAGISSFG ISGTNAHLLL 2000
    EQAPPAEEPA EEPAGDIEGA PLVPWLLSAR SAQALREQAS RLLTATTGTD 2050
    VTVEPARVGH ALATTRTAFD HRAVVLGSRP EDFTRGLEAV VSSASAPGVV 2100
    TGTAGPDSTG PVFVYPGQGS QWWGMGRELL AGSEVFRTAV DDCAAALAPY 2150
    TDWSLHEVLA GEGDPELLER VDVVQPALFS MMVALTALWR SHGVEPAAVV 2200
    GHSQGEIAAA HVAGALSLED AALVVALRSQ ALPQLSGLGG MMSVSAPAAR 2250
    VTELLQPWGE ALSIAAVNGP SSVIVSGDAD ALDELLETCR EQNVRARKVS 2300
    VDYASHGRHV EAVRDELARV LAPVSPRTPE VPFYSTVTGA RLDEAAFDGT 2350
    YWYTNLRQTV LMEDATRALI ASGHRVFVEI SPHPVLAAPI QETQESVQEE 2400
    LTETGAVLGT LRRDDGDRRR FLTSLAEAHT HGIAVDWTTA FDRPAYAAVD 2450
    LPTYPFQRQD FWPEPKTAAP AGTADASDAA FWELVQNQDL DALAGELGIP 2500
    ADDGAARLGD VLPALSAWRT RSLTRSRADA LRYHIEWNRV AEPGTARPAG 2550
    RLLAVISPDH AGAPWVTAVL DALGPDTVRF EAKGTDRAAW AAQLAQLRED 2600
    EGEFHAVVSL LAAAEALHTD HGSVPLGLAQ TLLLAQALGD AGLTAPLWCL 2650
    TRGGVAAGRG DVLSSPVQGA LWGLGRVIGL EHPDRWGGLI DLPETVDTRA 2700
    AARLTGLLAD AGGEDQLAIR GSGVLARRLA HAAPAVPGSG KRPPVHGSVL 2750
    VTGGTGGIGG RVARRLAEQG AAHLVLTSRR GADAPGAAEL RAELEQLGVR 2800
    VTIAACDAAD REALAALLAE LPEDAPLTAV FHSAGVAHDD APVADLTLGQ 2850
    LDALMRAKLT AARHLHELTA DLDLDAFVLF SSGAAVWGSG GQPGYAAANA 2900
    YLDALAEHRR SLGLTASSVA WGTWGEVGMA TDPEVHDRLV RQGVLAMEPE 2950
    HALGALDQML ENDDTAAAIT LMDWEMFAPA FTANRPSALL STVPEAVSAL 3000
    SDEGPADGGA DAAVPPLRAR LDGLPTAERA RALVEAVRAE ASATLGHDSP 3050
    DAVPASRAFR DVGFDSVTAV ELRNRLRAAL GLPLPAALVF DYPTPTALAQ 3100
    HIGSLLYGTA PDEADTGRAD DPDARIREAL ATVPIGRLRK AGLLDMVLKL 3150
    AESDDTEDSA TATDDAADAL DLDDMDAEAL LRLAAENSAN 3190
    Length:3,190
    Mass (Da):334,066
    Last modified:December 1, 2001 - v1
    Checksum:iEC005DABF792D89B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF357202 Genomic DNA. Translation: AAK73513.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF357202 Genomic DNA. Translation: AAK73513.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3MJC X-ray 1.48 A/B 2529-3003 [» ]
    3MJE X-ray 1.36 A/B 2529-3003 [» ]
    3MJS X-ray 1.40 A/B 2529-3003 [» ]
    3MJT X-ray 1.60 A/B 2529-3003 [» ]
    3MJV X-ray 1.46 A/B 2529-3003 [» ]
    4DIF X-ray 1.52 A/B 2529-3003 [» ]
    ProteinModelPortali Q93NW7.
    SMRi Q93NW7. Positions 9-922, 1462-1552, 3015-3105.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59008N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q93NW7.

    Family and domain databases

    Gene3Di 1.10.1200.10. 2 hits.
    3.40.366.10. 4 hits.
    3.40.47.10. 4 hits.
    3.40.50.720. 2 hits.
    InterProi IPR001227. Ac_transferase_dom.
    IPR009081. Acyl_carrier_prot-like.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR002198. DH_sc/Rdtase_SDR.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016036. Malonyl_transacylase_ACP-bd.
    IPR016040. NAD(P)-bd_dom.
    IPR020842. PKS/FAS_KR.
    IPR020801. PKS_acyl_transferase.
    IPR020841. PKS_Beta-ketoAc_synthase_dom.
    IPR013968. PKS_KR.
    IPR020806. PKS_PP-bd.
    IPR015083. Polyketide_synth_docking.
    IPR006162. PPantetheine_attach_site.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF00698. Acyl_transf_1. 2 hits.
    PF00106. adh_short. 1 hit.
    PF08990. Docking. 1 hit.
    PF00109. ketoacyl-synt. 2 hits.
    PF02801. Ketoacyl-synt_C. 2 hits.
    PF08659. KR. 1 hit.
    PF00550. PP-binding. 2 hits.
    [Graphical view ]
    SMARTi SM00827. PKS_AT. 2 hits.
    SM00822. PKS_KR. 2 hits.
    SM00825. PKS_KS. 2 hits.
    SM00823. PKS_PP. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47336. SSF47336. 2 hits.
    SSF52151. SSF52151. 4 hits.
    SSF53901. SSF53901. 2 hits.
    SSF55048. SSF55048. 2 hits.
    PROSITEi PS50075. ACP_DOMAIN. 2 hits.
    PS00606. B_KETOACYL_SYNTHASE. 2 hits.
    PS00012. PHOSPHOPANTETHEINE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes."
      Caffrey P., Lynch S., Flood E., Finnan S., Oliynyk M.
      Chem. Biol. 8:713-723(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Biosynthesis of amphotericin derivatives lacking exocyclic carboxyl groups."
      Carmody M., Murphy B., Byrne B., Power P., Rai D., Rawlings B., Caffrey P.
      J. Biol. Chem. 280:34420-34426(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    3. "Structural and functional analysis of A-type ketoreductases from the amphotericin modular polyketide synthase."
      Zheng J., Taylor C.A., Piasecki S.K., Keatinge-Clay A.T.
      Structure 18:913-922(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.36 ANGSTROMS) OF 2529-3003 IN COMPLEX WITH NADP.
    4. "Structure and mutagenesis of A2-type ketoreductase from modular polyketide synthase reveals insights into stereospecificity."
      Zheng J., Keatinge-Clay A.T.
      Submitted (JAN-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.52 ANGSTROMS) OF 2529-3003 IN COMPLEX WITH NADP.

    Entry informationi

    Entry nameiQ93NW7_9ACTO
    AccessioniPrimary (citable) accession number: Q93NW7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: December 1, 2001
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3