Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q93NW7 (Q93NW7_9ACTO) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein names
Gene names
Name:amphB EMBL AAK73513.1
OrganismStreptomyces nodosus EMBL AAK73513.1
Taxonomic identifier40318 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length3190 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding2756 – 27583NADP PDB 4DIF PDB 3MJE PDB 3MJS PDB 3MJT PDB 3MJV
Nucleotide binding2779 – 27802NADP PDB 4DIF PDB 3MJE PDB 3MJS PDB 3MJT PDB 3MJV
Nucleotide binding2807 – 28082NADP PDB 4DIF PDB 3MJE PDB 3MJS PDB 3MJT PDB 3MJV
Nucleotide binding2833 – 28353NADP PDB 4DIF PDB 3MJE PDB 3MJS PDB 3MJT PDB 3MJV

Sites

Binding site28581NADP PDB 4DIF PDB 3MJE PDB 3MJS PDB 3MJT PDB 3MJV
Binding site28951NADP PDB 4DIF PDB 3MJE PDB 3MJS PDB 3MJT PDB 3MJV
Binding site29241NADP; via amide nitrogen PDB 4DIF PDB 3MJE PDB 3MJS PDB 3MJT PDB 3MJV
Binding site29291NADP; via amide nitrogen PDB 4DIF PDB 3MJE PDB 3MJS PDB 3MJT PDB 3MJV

Sequences

Sequence LengthMass (Da)Tools
Q93NW7 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: EC005DABF792D89B

FASTA3,190334,066
        10         20         30         40         50         60 
MPEPQQNQQE KVVDYLRRVT NDLRRARRRI GELESRDNEP VAIVGMSCRL PGGVSSPESL 

        70         80         90        100        110        120 
WELVESGGDA ISGFPADRGW DIEALTGKGE GSSSTHEGGF LYEATDFDAG FFGISPREAT 

       130        140        150        160        170        180 
AMDPQQRLLL EVSWEALERA GIAPTSLRSS ATGVFVGSYH WGAPSADTAT ELHGHALTGT 

       190        200        210        220        230        240 
AASVLSGRLS YVYGLEGPAV TVDTACSSSL VALHMAAQSL RNGESSLALV GGVTILAEPS 

       250        260        270        280        290        300 
VFVEFSAQGG LAQDGRCKAF SDSADGTGWS EGAAVLVVER LSDARRAGHR VLAVVRGSAV 

       310        320        330        340        350        360 
NQDGASNGLT APNGPSQQRV IRQALANAGL RPGDVDAVEA HGTGTPLGDP IEAQALIATY 

       370        380        390        400        410        420 
GSDRDPQQPL LLGSVKSNIG HTQSAAGVAG LVKMVMAMHN GTLPRTLHLT EPSTHVDWSL 

       430        440        450        460        470        480 
GAVRLLTEET AWPETGRVRR AAVSSFGISG TNAHAVLEQA PVPAEDAEDA GTEDAPQQPA 

       490        500        510        520        530        540 
TVPGALPWLL SAHSGQALCD QAAALVTRLD ADPEPRPQDI GQGLLTSRAL LERRAVVVGT 

       550        560        570        580        590        600 
GRDELLTGSR ALAEGQSWNG LAEGTADVDG RTVFVFPGQG SQWVGMGARL LDESPVFAER 

       610        620        630        640        650        660 
IAECAAALAE FTDWDLLDVL RGAEGAPTLE RVDVVQPASF AVMVSLAAVW RAQGVEPDAV 

       670        680        690        700        710        720 
VGHSQGEIAA AVVSGALSLR DGARVVALRS QAIGRTLAGR GGMMSVALPV ADVEARLEAF 

       730        740        750        760        770        780 
GGRVSVAAEN GPRSCVVSGE PGALDELSEL LTAEGVRVRR VPVDYASHSH HVEDLHDEIL 

       790        800        810        820        830        840 
QLLAEVAPKA SEVPLFSTVT GDWLDTTVMD AGYWFRSLRG RVLFADAIRG LLDTEHRAFI 

       850        860        870        880        890        900 
EVSSHPVLAM SVQDMIDDAG VVGVASGTLR RDNGGLDRFL LSAAQVFVRG VQVDWAPLFE 

       910        920        930        940        950        960 
GTGATRVDLP TYAFQHERYW NDRAVVDRSA SAPMDAEFWA AVEQADVSAL TEALGTDEES 

       970        980        990       1000       1010       1020 
VAAILPGLAS WRRTRGERST VDSWRYRVVW EPLAQIPRAT LDGTWLLVSA GGVDDDVAEV 

      1030       1040       1050       1060       1070       1080 
LEAGGAEVRR LVLDETCTDP AVLREHLTDT DGLTGIVSVL ADAEEGCVRH PGLTLGLALS 

      1090       1100       1110       1120       1130       1140 
VSLAQALGEA DVTAPLWFLT CGAFSTGPSD TVTRPLQSQI AGLGWTVAVE HPHRWGGGVD 

      1150       1160       1170       1180       1190       1200 
LPEALDARAA QRLVAALAGA LGDDDQLAVR PAGVLARRIV RASGDTRRKA RSWKPRGTTL 

      1210       1220       1230       1240       1250       1260 
VTGGSGTLAP GLARHLAAQG AEHLVLLSRR GADAPGAAEL AAELQAAGTE VRFAACDITD 

      1270       1280       1290       1300       1310       1320 
PDAVAALLAD LKAEGRTVRT VVHTAAVIEL AALADTTVDA FADVVHAKVT GARILDELLD 

      1330       1340       1350       1360       1370       1380 
DEELDDFVLY SSTAGMWGSG VHAAYVAGNA YLSALAEQRR ARGARATSIH WGKWPDDLER 

      1390       1400       1410       1420       1430       1440 
ELADPHQIRR SGLEYLDPEL AMTALTRVME DDETVIGLMD IDWGTYHDVF TAGRPSHLFD 

      1450       1460       1470       1480       1490       1500 
RIPEVARLLA DRAAPAATAV ATSGLAARLQ GVSAAEQDRI VLSVVREETA AVLGHASAET 

      1510       1520       1530       1540       1550       1560 
VPERRAFRDI GFDSVTAVDL RNRLVAATGL TLPSTMVFDH PNAVALATFL KATALGTTGT 

      1570       1580       1590       1600       1610       1620 
AGDRPTAAVT AGADDDLIVI VGMSCRFPGG ANTPEELLRL ALDGADVISE FPADRGWDAH 

      1630       1640       1650       1660       1670       1680 
GLYDPDPDRQ GRTYSVHGGF LHEAAGFDAG FFGISPREAL AMDPQQRLLL ETSWEVLERA 

      1690       1700       1710       1720       1730       1740 
GIDPESLHST ATGAFFGASY QDYSSSVQNG TEGAEVHMMT GTAASVLSGR ISYLLGLEGP 

      1750       1760       1770       1780       1790       1800 
AVTVDTACSS SLVAMHLACQ SLRSGESSLA LAGGAAVMAT PHAFIGFSRQ RALAENGRCK 

      1810       1820       1830       1840       1850       1860 
PFAEGADGMT LAEGVGVVLL EKLSDARRNG HRVLAVVRGS ALNQDGASNG LTAPNGLSQQ 

      1870       1880       1890       1900       1910       1920 
RVIRQALANA GLGTADIDVV EAHGTGTKLG DPIEAGALLA TYGQGRDGGQ PLLLGSVKSN 

      1930       1940       1950       1960       1970       1980 
IGHTQAAAGV AGVIKMVMAM HAGELPGTLH IDEPSSHVDW TSGAVTLLRE RTEWPAVGDR 

      1990       2000       2010       2020       2030       2040 
PRRAGISSFG ISGTNAHLLL EQAPPAEEPA EEPAGDIEGA PLVPWLLSAR SAQALREQAS 

      2050       2060       2070       2080       2090       2100 
RLLTATTGTD VTVEPARVGH ALATTRTAFD HRAVVLGSRP EDFTRGLEAV VSSASAPGVV 

      2110       2120       2130       2140       2150       2160 
TGTAGPDSTG PVFVYPGQGS QWWGMGRELL AGSEVFRTAV DDCAAALAPY TDWSLHEVLA 

      2170       2180       2190       2200       2210       2220 
GEGDPELLER VDVVQPALFS MMVALTALWR SHGVEPAAVV GHSQGEIAAA HVAGALSLED 

      2230       2240       2250       2260       2270       2280 
AALVVALRSQ ALPQLSGLGG MMSVSAPAAR VTELLQPWGE ALSIAAVNGP SSVIVSGDAD 

      2290       2300       2310       2320       2330       2340 
ALDELLETCR EQNVRARKVS VDYASHGRHV EAVRDELARV LAPVSPRTPE VPFYSTVTGA 

      2350       2360       2370       2380       2390       2400 
RLDEAAFDGT YWYTNLRQTV LMEDATRALI ASGHRVFVEI SPHPVLAAPI QETQESVQEE 

      2410       2420       2430       2440       2450       2460 
LTETGAVLGT LRRDDGDRRR FLTSLAEAHT HGIAVDWTTA FDRPAYAAVD LPTYPFQRQD 

      2470       2480       2490       2500       2510       2520 
FWPEPKTAAP AGTADASDAA FWELVQNQDL DALAGELGIP ADDGAARLGD VLPALSAWRT 

      2530       2540       2550       2560       2570       2580 
RSLTRSRADA LRYHIEWNRV AEPGTARPAG RLLAVISPDH AGAPWVTAVL DALGPDTVRF 

      2590       2600       2610       2620       2630       2640 
EAKGTDRAAW AAQLAQLRED EGEFHAVVSL LAAAEALHTD HGSVPLGLAQ TLLLAQALGD 

      2650       2660       2670       2680       2690       2700 
AGLTAPLWCL TRGGVAAGRG DVLSSPVQGA LWGLGRVIGL EHPDRWGGLI DLPETVDTRA 

      2710       2720       2730       2740       2750       2760 
AARLTGLLAD AGGEDQLAIR GSGVLARRLA HAAPAVPGSG KRPPVHGSVL VTGGTGGIGG 

      2770       2780       2790       2800       2810       2820 
RVARRLAEQG AAHLVLTSRR GADAPGAAEL RAELEQLGVR VTIAACDAAD REALAALLAE 

      2830       2840       2850       2860       2870       2880 
LPEDAPLTAV FHSAGVAHDD APVADLTLGQ LDALMRAKLT AARHLHELTA DLDLDAFVLF 

      2890       2900       2910       2920       2930       2940 
SSGAAVWGSG GQPGYAAANA YLDALAEHRR SLGLTASSVA WGTWGEVGMA TDPEVHDRLV 

      2950       2960       2970       2980       2990       3000 
RQGVLAMEPE HALGALDQML ENDDTAAAIT LMDWEMFAPA FTANRPSALL STVPEAVSAL 

      3010       3020       3030       3040       3050       3060 
SDEGPADGGA DAAVPPLRAR LDGLPTAERA RALVEAVRAE ASATLGHDSP DAVPASRAFR 

      3070       3080       3090       3100       3110       3120 
DVGFDSVTAV ELRNRLRAAL GLPLPAALVF DYPTPTALAQ HIGSLLYGTA PDEADTGRAD 

      3130       3140       3150       3160       3170       3180 
DPDARIREAL ATVPIGRLRK AGLLDMVLKL AESDDTEDSA TATDDAADAL DLDDMDAEAL 

      3190 
LRLAAENSAN 

« Hide

References

[1]"Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes."
Caffrey P., Lynch S., Flood E., Finnan S., Oliynyk M.
Chem. Biol. 8:713-723(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Biosynthesis of amphotericin derivatives lacking exocyclic carboxyl groups."
Carmody M., Murphy B., Byrne B., Power P., Rai D., Rawlings B., Caffrey P.
J. Biol. Chem. 280:34420-34426(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[3]"Structural and functional analysis of A-type ketoreductases from the amphotericin modular polyketide synthase."
Zheng J., Taylor C.A., Piasecki S.K., Keatinge-Clay A.T.
Structure 18:913-922(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.36 ANGSTROMS) OF 2529-3003 IN COMPLEX WITH NADP.
[4]"Structure and mutagenesis of A2-type ketoreductase from modular polyketide synthase reveals insights into stereospecificity."
Zheng J., Keatinge-Clay A.T.
Submitted (JAN-2012) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.52 ANGSTROMS) OF 2529-3003 IN COMPLEX WITH NADP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF357202 Genomic DNA. Translation: AAK73513.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3MJCX-ray1.48A/B2529-3003[»]
3MJEX-ray1.36A/B2529-3003[»]
3MJSX-ray1.40A/B2529-3003[»]
3MJTX-ray1.60A/B2529-3003[»]
3MJVX-ray1.46A/B2529-3003[»]
4DIFX-ray1.52A/B2529-3003[»]
ProteinModelPortalQ93NW7.
SMRQ93NW7. Positions 9-922, 1462-1552, 3015-3105.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59008N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1200.10. 2 hits.
3.40.366.10. 4 hits.
3.40.47.10. 4 hits.
3.40.50.720. 2 hits.
InterProIPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR002198. DH_sc/Rdtase_SDR.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR016040. NAD(P)-bd_dom.
IPR020842. PKS/FAS_KR.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR013968. PKS_KR.
IPR020806. PKS_PP-bd.
IPR015083. Polyketide_synth_docking.
IPR006162. PPantetheine_attach_site.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF00698. Acyl_transf_1. 2 hits.
PF00106. adh_short. 1 hit.
PF08990. Docking. 1 hit.
PF00109. ketoacyl-synt. 2 hits.
PF02801. Ketoacyl-synt_C. 2 hits.
PF08659. KR. 1 hit.
PF00550. PP-binding. 2 hits.
[Graphical view]
SMARTSM00827. PKS_AT. 2 hits.
SM00822. PKS_KR. 2 hits.
SM00825. PKS_KS. 2 hits.
SM00823. PKS_PP. 2 hits.
[Graphical view]
SUPFAMSSF47336. SSF47336. 2 hits.
SSF52151. SSF52151. 4 hits.
SSF53901. SSF53901. 2 hits.
SSF55048. SSF55048. 2 hits.
PROSITEPS50075. ACP_DOMAIN. 2 hits.
PS00606. B_KETOACYL_SYNTHASE. 2 hits.
PS00012. PHOSPHOPANTETHEINE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ93NW7.

Entry information

Entry nameQ93NW7_9ACTO
AccessionPrimary (citable) accession number: Q93NW7
Entry history
Integrated into UniProtKB/TrEMBL: December 1, 2001
Last sequence update: December 1, 2001
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)