Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein
Submitted name:

6-hydroxy-L-nicotine oxidase

Gene

6-hlno

Organism
Arthrobacter nicotinovorans
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei226 – 2261FAD; via amide nitrogen and carbonyl oxygenCombined sources
Binding sitei398 – 3981FADCombined sources

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 122FADCombined sources
Nucleotide bindingi31 – 322FADCombined sources
Nucleotide bindingi37 – 393FADCombined sources
Nucleotide bindingi56 – 594FADCombined sources
Nucleotide bindingi406 – 4083FADCombined sources

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseImported

Keywords - Ligandi

FADCombined sources, Flavoprotein, Nucleotide-bindingCombined sources

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-964.
BRENDAi1.5.3.5. 449.
1.5.99.14. 449.
1.5.99.4. 449.

Names & Taxonomyi

Protein namesi
Submitted name:
6-hydroxy-L-nicotine oxidaseImported (EC:1.5.3.5Imported)
Gene namesi
Name:6-hlnoImported
Encoded oniPlasmid pA01Imported
Plasmid pAO1Imported
OrganismiArthrobacter nicotinovoransImported
Taxonomic identifieri29320 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaePaenarthrobacter

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3K7MX-ray1.95X1-425[»]
3K7QX-ray2.05X1-425[»]
3K7TX-ray2.85A/B1-425[»]
3NG7X-ray1.95X1-425[»]
3NGCX-ray2.25X1-425[»]
3NH3X-ray2.10X1-425[»]
3NHOX-ray2.85X1-425[»]
3NK0X-ray2.15X1-425[»]
3NK1X-ray2.20X1-425[»]
3NK2X-ray2.65X1-425[»]
3NN0X-ray2.75X1-425[»]
3NN6X-ray2.19X1-425[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 423413Amino_oxidaseInterPro annotationAdd
BLAST

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR002937. Amino_oxidase.
IPR023753. FAD/NAD-binding_dom.
IPR001613. Flavin_amine_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
PRINTSiPR00757. AMINEOXDASEF.
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

Q93NH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYDAIVVGGG FSGLKAARDL TNAGKKVLLL EGGERLGGRA YSRESRNVPG
60 70 80 90 100
LRVEIGGAYL HRKHHPRLAA ELDRYGIPTA AASEFTSFRH RLGPTAVDQA
110 120 130 140 150
FPIPGSEAVA VEAATYTLLR DAHRIDLEKG LENQDLEDLD IPLNEYVDKL
160 170 180 190 200
DLPPVSRQFL LAWAWNMLGQ PADQASALWM LQLVAAHHYS ILGVVLSLDE
210 220 230 240 250
VFSNGSADLV DAMSQEIPEI RLQTVVTGID QSGDVVNVTV KDGHAFQAHS
260 270 280 290 300
VIVATPMNTW RRIVFTPALP ERRRSVIEEG HGGQGLKILI HVRGAEAGIE
310 320 330 340 350
CVGDGIFPTL YDYCEVSESE RLLVAFTDSG SFDPTDIGAV KDAVLYYLPE
360 370 380 390 400
VEVLGIDYHD WIADPLFEGP WVAPRVGQFS RVHKELGEPA GRIHFVGSDV
410 420
SLEFPGYIEG ALETAECAVN AILHS
Length:425
Mass (Da):46,335
Last modified:December 1, 2001 - v1
Checksum:i7B358BFFD90B1BA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF373840 Genomic DNA. Translation: AAK64245.1.
AJ507836 Genomic DNA. Translation: CAD47951.1.
RefSeqiWP_016359462.1. NC_021229.1.
YP_007988777.1. NC_021229.1.

Genome annotation databases

GeneIDi15879608.
KEGGipg:15879608.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF373840 Genomic DNA. Translation: AAK64245.1.
AJ507836 Genomic DNA. Translation: CAD47951.1.
RefSeqiWP_016359462.1. NC_021229.1.
YP_007988777.1. NC_021229.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3K7MX-ray1.95X1-425[»]
3K7QX-ray2.05X1-425[»]
3K7TX-ray2.85A/B1-425[»]
3NG7X-ray1.95X1-425[»]
3NGCX-ray2.25X1-425[»]
3NH3X-ray2.10X1-425[»]
3NHOX-ray2.85X1-425[»]
3NK0X-ray2.15X1-425[»]
3NK1X-ray2.20X1-425[»]
3NK2X-ray2.65X1-425[»]
3NN0X-ray2.75X1-425[»]
3NN6X-ray2.19X1-425[»]
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi15879608.
KEGGipg:15879608.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-964.
BRENDAi1.5.3.5. 449.
1.5.99.14. 449.
1.5.99.4. 449.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR002937. Amino_oxidase.
IPR023753. FAD/NAD-binding_dom.
IPR001613. Flavin_amine_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
PRINTSiPR00757. AMINEOXDASEF.
SUPFAMiSSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "6-hydroxy-D-nicotine oxidase of Arthrobacter oxidans. Gene structure of the flavoenzyme and its relationship to 6-hydroxy-L-nicotine oxidase."
    Brandsch R., Hinkkanen A.E., Mauch L., Nagursky H., Decker K.
    Eur. J. Biochem. 167:315-320(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 49919Imported.
    Plasmid: pAO1
  2. "Gene cluster on pAO1 of Arthrobacter nicotinovorans involved in degradation of the plant alkaloid nicotine: cloning, purification, and characterization of 2,6-dihydroxypyridine 3-hydroxylase."
    Baitsch D., Sandu C., Brandsch R., Igloi G.L.
    J. Bacteriol. 183:5262-5267(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Plasmid: pA01
  3. "Sequence of the 165-kilobase catabolic plasmid pAO1 from Arthrobacter nicotinovorans and identification of a pAO1-dependent nicotine uptake system."
    Igloi G.L., Brandsch R.
    J. Bacteriol. 185:1976-1986(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 49919Imported.
    Plasmid: pAO1
  4. "Crystal structure analysis of free and substrate-bound 6-hydroxy-L-nicotine oxidase from Arthrobacter nicotinovorans."
    Kachalova G.S., Bourenkov G.P., Mengesdorf T., Schenk S., Maun H.R., Burghammer M., Riekel C., Decker K., Bartunik H.D.
    J. Mol. Biol. 396:785-799(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH FAD.
  5. "Crystallographic snapshots of the complete reaction cycle of nicotine degradation by an amine oxidase of the monoamine oxidase (MAO) family."
    Kachalova G., Decker K., Holt A., Bartunik H.D.
    Proc. Natl. Acad. Sci. U.S.A. 108:4800-4805(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH FAD.
  6. "pAO1 of Arthrobacter nicotinovorans and the spread of catabolic traits by horizontal gene transfer in gram-positive soil bacteria."
    Mihasan M., Brandsch R.
    J. Mol. Evol. 77:22-30(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 49919Imported.
    Plasmid: pAO1

Entry informationi

Entry nameiQ93NH4_ARTNI
AccessioniPrimary (citable) accession number: Q93NH4
Entry historyi
Integrated into UniProtKB/TrEMBL: December 1, 2001
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, PlasmidImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.