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Protein

2,6-dihydropseudooxynicotine hydrolase

Gene
N/A
Organism
Arthrobacter nicotinovorans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

L-nicotine is used as a growth substrate. Plays a role in nicotine catabolism by cleaving a C-C bond in 2,6-dihydroxypseudooxynicotine.2 Publications

Catalytic activityi

1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O = 2,6-dihydroxypyridine + 4-methylaminobutanoate.2 Publications

Kineticsi

  1. KM=6 µM for 2,6-dihydropseudooxynicotine1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Pathwayi: nicotine degradation

    This protein is involved in step 2 of the subpathway that synthesizes 2,6-dihydroxypyridine and 4-(methylamino)butanoate from 6-hydroxypseudooxynicotine.3 Publications
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. 2,6-dihydropseudooxynicotine hydrolase
    This subpathway is part of the pathway nicotine degradation, which is itself part of Alkaloid degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2,6-dihydroxypyridine and 4-(methylamino)butanoate from 6-hydroxypseudooxynicotine, the pathway nicotine degradation and in Alkaloid degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei1481 PublicationSequence analysis1
    Active sitei2171 Publication1
    Active sitei3001 Publication1
    Active sitei3291 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Alkaloid metabolism

    Enzyme and pathway databases

    BRENDAi3.7.1.19. 449.
    SABIO-RKQ93NG6.
    UniPathwayiUPA00106; UER00490.

    Protein family/group databases

    ESTHERiartni-Q93NG6. Duf_1100.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2,6-dihydropseudooxynicotine hydrolase (EC:3.7.1.19)
    Encoded oniPlasmid pAO1Imported
    OrganismiArthrobacter nicotinovorans
    Taxonomic identifieri29320 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaePaenarthrobacter

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi18R → A: Total loss of activity. 1 Publication1
    Mutagenesisi22D → A: 91% loss of activity. 1 Publication1
    Mutagenesisi148E → A: 98% loss of activity. 1 Publication1
    Mutagenesisi149S → A: 84% loss of activity. 1 Publication1
    Mutagenesisi153E → A: 32% loss of activity. 1 Publication1
    Mutagenesisi216R → A: 92% loss of activity. 1 Publication1
    Mutagenesisi217S → A: Total loss of activity. 1 Publication1
    Mutagenesisi300D → A: 94% loss of activity. 1 Publication1
    Mutagenesisi329H → A: 92% loss of activity. 1 Publication1
    Mutagenesisi330C → A: 5% loss of activity. 1 Publication1
    Mutagenesisi332H → A: 58% loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002837351 – 3672,6-dihydropseudooxynicotine hydrolaseAdd BLAST367

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    Secondary structure

    1367
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi9 – 13Combined sources5
    Helixi16 – 22Combined sources7
    Helixi26 – 33Combined sources8
    Helixi41 – 61Combined sources21
    Helixi65 – 83Combined sources19
    Helixi90 – 105Combined sources16
    Helixi106 – 108Combined sources3
    Beta strandi109 – 111Combined sources3
    Beta strandi113 – 120Combined sources8
    Beta strandi123 – 130Combined sources8
    Beta strandi133 – 135Combined sources3
    Beta strandi138 – 144Combined sources7
    Turni151 – 153Combined sources3
    Helixi155 – 163Combined sources9
    Beta strandi167 – 171Combined sources5
    Helixi177 – 179Combined sources3
    Turni180 – 182Combined sources3
    Helixi189 – 202Combined sources14
    Beta strandi206 – 216Combined sources11
    Helixi218 – 229Combined sources12
    Beta strandi235 – 240Combined sources6
    Helixi248 – 250Combined sources3
    Helixi253 – 262Combined sources10
    Helixi268 – 278Combined sources11
    Turni282 – 284Combined sources3
    Helixi285 – 287Combined sources3
    Beta strandi292 – 297Combined sources6
    Beta strandi300 – 302Combined sources3
    Helixi305 – 313Combined sources9
    Helixi316 – 318Combined sources3
    Beta strandi319 – 324Combined sources6
    Helixi329 – 334Combined sources6
    Helixi337 – 350Combined sources14
    Beta strandi360 – 362Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2JBWX-ray2.10A/B/C/D1-365[»]
    ProteinModelPortaliQ93NG6.
    SMRiQ93NG6.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ93NG6.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UPF0255 family.Sequence analysis

    Phylogenomic databases

    KOiK19188.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR010520. UPF0255.
    [Graphical view]
    PfamiPF06500. DUF1100. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q93NG6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTVTSQVKPE DEMLNWGRLI LDGVSYSDMV GARDRPKEIT WFDYWMSLAN
    60 70 80 90 100
    EYEQEAERKV ALGHDLSAGE LLMSAALCAQ YAQFLWFDER RQKGQARKVE
    110 120 130 140 150
    LYQKAAPLLS PPAERHELVV DGIPMPVYVR IPEGPGPHPA VIMLGGLEST
    160 170 180 190 200
    KEESFQMENL VLDRGMATAT FDGPGQGEMF EYKRIAGDYE KYTSAVVDLL
    210 220 230 240 250
    TKLEAIRNDA IGVLGRSLGG NYALKSAACE PRLAACISWG GFSDLDYWDL
    260 270 280 290 300
    ETPLTKESWK YVSKVDTLEE ARLHVHAALE TRDVLSQIAC PTYILHGVHD
    310 320 330 340 350
    EVPLSFVDTV LELVPAEHLN LVVEKDGDHC CHNLGIRPRL EMADWLYDVL
    360
    VAGKKVAPTM KGWPLNG
    Length:367
    Mass (Da):40,995
    Last modified:December 1, 2001 - v1
    Checksum:i1A5D31A7DFC4C8E3
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF373840 Genomic DNA. Translation: AAK64252.1.
    AJ507836 Genomic DNA. Translation: CAD47941.1.
    RefSeqiWP_016359452.1. NC_021229.1.
    YP_007988767.1. NC_021229.1.

    Genome annotation databases

    GeneIDi15879627.
    KEGGiag:CAD47941.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF373840 Genomic DNA. Translation: AAK64252.1.
    AJ507836 Genomic DNA. Translation: CAD47941.1.
    RefSeqiWP_016359452.1. NC_021229.1.
    YP_007988767.1. NC_021229.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2JBWX-ray2.10A/B/C/D1-365[»]
    ProteinModelPortaliQ93NG6.
    SMRiQ93NG6.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    ESTHERiartni-Q93NG6. Duf_1100.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi15879627.
    KEGGiag:CAD47941.

    Phylogenomic databases

    KOiK19188.

    Enzyme and pathway databases

    UniPathwayiUPA00106; UER00490.
    BRENDAi3.7.1.19. 449.
    SABIO-RKQ93NG6.

    Miscellaneous databases

    EvolutionaryTraceiQ93NG6.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR010520. UPF0255.
    [Graphical view]
    PfamiPF06500. DUF1100. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDHPON_ARTNI
    AccessioniPrimary (citable) accession number: Q93NG6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: December 1, 2001
    Last modified: November 30, 2016
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Plasmid

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.