Reviewed, UniProtKB/Swiss-Prot Q93LR4 (K6PF_BACSH)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase
      Short name=Phosphofructokinase
    EC=2.7.1.11
Alternative name(s):
    Phosphohexokinase

Gene names

Name:	pfkA
Synonyms:	pfk

Organism

Bacillus sphaericus

Taxonomic identifier

1421 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Planococcaceae › Lysinibacillus

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	6-phosphofructokinase HAMAP MF_00339		PRO_0000111934
Regions
	Nucleotide binding	21 – 25	5	ATP By similarity
	Nucleotide binding	154 – 158	5	ATP By similarity
	Nucleotide binding	171 – 187	17	ATP By similarity
Sites
	Active site	127	1	Proton acceptor By similarity
	Metal binding	185	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	187	1	Magnesium By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q93LR4-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 77FF47BF43E5E23B	FASTA	319	33,987
	10 20 30 40 50 60 MKKIAVLTSG GDVPGMNAAI RAVVRKAAFH GINVVGIKHG YEGLVKGYLE ELDLGSVGGI 70 80 90 100 110 120 IQRGGTHLNS ARCPEFKEDA VQQQGIENLR AAGIEGLVVI GGDGSYRGAM DLVKKGFPAV 130 140 150 160 170 180 GVPGTIDNDV PGTEYTIGFD TALNTVVESI DKIRDTATSH ENSFIVEVMG RDAGDIALWA 190 200 210 220 230 240 GLAAGAETVL IPEEDYDLDD IVARLDRGAA RGKKHSIIIV AEGVMSGNEL AKLIKEKTGK 250 260 270 280 290 300 ETRVSVLGHI QRGGSPTARD RVLASQFGAH AVELLMEGKY GRAVGIRNHQ VIDYDMPEAF 310 EKNHEADVSL YTLMKELSI

References

[1]	"6-phosphofructokinase of Bacillus sphaericus 2362." Alice A.F., Perez Martinez G., Sanchez Rivas C. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 2362.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY034597 Genomic DNA. Translation: AAK57729.1.
3D structure databases
HSSP	HSSP built from PDB template 6PFK based on UniProtKB P00512.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.11. 327.
Family and domain databases
HAMAP	MF_00339. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProDom	PD000707. Ppfruckinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_BACSH

Accession

Primary (citable) accession number: Q93LR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)