Reviewed, UniProtKB/Swiss-Prot Q93JT2 (TPMT_PSEU5)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:	tpm
Synonyms:	tmpA
Ordered Locus Names:	PST_1312

Organism

Pseudomonas stutzeri (strain A1501) [Complete proteome] [HAMAP]

Taxonomic identifier

379731 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_0000220131
Sites
	Binding site	13	1	S-adenosyl-L-methionine By similarity
	Binding site	48	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	69	1	S-adenosyl-L-methionine By similarity
	Binding site	126	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q93JT2-1 [UniParc]. Last modified September 13, 2005. Version 3. Checksum: 410021AD19465711	FASTA	235	26,502
	10 20 30 40 50 60 MTDHDNQRWL QLWRERRTDF HQHGVNLLLS RFWPAFAPAT PSRVFVPLCG KSLDMLWLAE 70 80 90 100 110 120 QGHDVIGVEL SPLAIEAFFR ENHLPPSKRR QGRFTLWRHG RIGILCGDYF ALSEADLGPV 130 140 150 160 170 180 DSVYDRAALT ALPPILRSRY VAQLRRIVPD TARVFLLTLE DAEADATLQQ ALGVDEELAA 190 200 210 220 230 LYTAGFEIAL AHVESLFEPD PQNGAPRRVE HKVYQLTGKR PASPEADGRA AETED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nitrogen fixation genetics and regulation in a Pseudomonas stutzeri strain associated with rice." Desnoues N., Lin M., Guo X., Ma L., Carreno-Lopez R., Elmerich C. Microbiology 149:2251-2262(2003) [PubMed: 12904565] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of the metabolically versatile and root-associated nitrogen-fixing bacterium Pseudomonas stutzeri A1501." Yan Y., Yang J., Dou Y., Ping S., Chen M., Yao Z., Li H., Lu W., Zhang W., Peng J., Liu W., He S., Geng L., Zhang X., Yang F., Li D., Lin Z., Wang Y. , Elmerich C., Lin M., Jin Q. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ297529 Genomic DNA. Translation: CAC44176.3. Different initiation. CP000304 Genomic DNA. Translation: ABP79007.1.
RefSeq	YP_001171849.1.
3D structure databases
HSSP	HSSP built from PDB template 1PJZ based on UniProtKB O86262.
ModBase	Search...
Protein-protein interaction databases
STRING	Q93JT2.
Genome annotation databases
GeneID	5097399.
GenomeReviews	Gene locus PST_1312 in contig CP000304_GR.
KEGG	psa:PST_1312.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TSIGHES.
Family and domain databases
HAMAP	MF_00812. [Tree]
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_PSEU5

Accession

Primary (citable) accession number: Q93JT2
Secondary accession number(s): A4VJ56

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 38 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)