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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79SubstrateUniRule annotation1
Metal bindingi233ManganeseUniRule annotation1
Metal bindingi253Manganese; via tele nitrogenUniRule annotation1
Binding sitei275SubstrateUniRule annotation1
Active sitei277UniRule annotation1
Metal bindingi300ManganeseUniRule annotation1
Binding sitei392GTPUniRule annotation1
Binding sitei423GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi276 – 281GTPUniRule annotation6
Nucleotide bindingi518 – 521GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:SCO4979
ORF Names:2SCK36.02
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036151 – 609Phosphoenolpyruvate carboxykinase [GTP]Add BLAST609

Proteomic databases

PRIDEiQ93JL5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO4979

Structurei

3D structure databases

ProteinModelPortaliQ93JL5
SMRiQ93JL5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni224 – 226Substrate bindingUniRule annotation3
Regioni390 – 392Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M Bacteria
COG1274 LUCA
HOGENOMiHOG000191700
InParanoidiQ93JL5
KOiK01596
OMAiCWLALTD
OrthoDBiPOG091H0B4F
PhylomeDBiQ93JL5

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

Q93JL5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARDIAAPPV PTNHQELISW VNEIAELTQP DAVVWCDGSE AEYERLCGEL
60 70 80 90 100
VEKGTFRKLD PIKRPNSYYA ASDPTDVARV EDRTFICSAK EEDAGPTNHW
110 120 130 140 150
KDPAEMRAIF TGDKGEGGLF RGSMRGRTMY VVPFCMGPLG SPLSALGVEI
160 170 180 190 200
TDSAYVAASM RTMTRMGQPV LDELGDEGFF VKAVHSVGAP LEPGQADVPW
210 220 230 240 250
PCNSTKYISH FPEDREIWSY GSGYGGNALL GKKCYALRIA SVMARDEGWL
260 270 280 290 300
AEHMLVLKLT PPTGAPKYVA AAFPSACGKT NLAMLEPTIS GWTVETIGDD
310 320 330 340 350
IAWMRFGEDG RLYAINPEAG FFGVAPGTGE HTNANAMKTL WGNSVFTNVA
360 370 380 390 400
LTDDGDVWWE GMTEETPAHL TDWKGNDWTP ESGTPAAHPN ARFTTPAAQC
410 420 430 440 450
PIIAPEWEDP RGVPISAILF GGRRATAVPL VTESFDWNHG VFLGANVASE
460 470 480 490 500
KTAAAEGKVG ELRRDPFAML PFCGYNMGDY MGHWVDVAKD KDQSKLPKIY
510 520 530 540 550
YVNWFRKDDA GRFVWPGFGE NGRVLKWIVE RLEGRADGVE TPIGVLPTKE
560 570 580 590 600
SLDTDGLDLA DADLEFLLSV DKEVWREEAA LVPEHLNTFG DHTPAELWDQ

YRALVRRLG
Length:609
Mass (Da):66,917
Last modified:December 1, 2001 - v1
Checksum:i80FF6E00B817875A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939122 Genomic DNA Translation: CAC40592.1
RefSeqiNP_629131.1, NC_003888.3
WP_003973998.1, NC_003888.3

Genome annotation databases

EnsemblBacteriaiCAC40592; CAC40592; CAC40592
GeneIDi1100420
KEGGisco:SCO4979
PATRICifig|100226.15.peg.5060

Similar proteinsi

Entry informationi

Entry nameiPCKG_STRCO
AccessioniPrimary (citable) accession number: Q93JL5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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