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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791SubstrateUniRule annotation
Binding sitei226 – 2261Substrate; via amide nitrogenUniRule annotation
Metal bindingi233 – 2331ManganeseUniRule annotation
Binding sitei233 – 2331SubstrateUniRule annotation
Metal bindingi253 – 2531Manganese; via tele nitrogenUniRule annotation
Binding sitei275 – 2751SubstrateUniRule annotation
Active sitei277 – 2771UniRule annotation
Metal bindingi300 – 3001ManganeseUniRule annotation
Binding sitei392 – 3921GTPUniRule annotation
Binding sitei423 – 4231GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi276 – 2816GTPUniRule annotation
Nucleotide bindingi518 – 5214GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:SCO4979
ORF Names:2SCK36.02
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 609609Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103615Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO4979.

Structurei

3D structure databases

ProteinModelPortaliQ93JL5.
SMRiQ93JL5. Positions 10-609.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni390 – 3923Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
InParanoidiQ93JL5.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.
PhylomeDBiQ93JL5.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q93JL5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARDIAAPPV PTNHQELISW VNEIAELTQP DAVVWCDGSE AEYERLCGEL
60 70 80 90 100
VEKGTFRKLD PIKRPNSYYA ASDPTDVARV EDRTFICSAK EEDAGPTNHW
110 120 130 140 150
KDPAEMRAIF TGDKGEGGLF RGSMRGRTMY VVPFCMGPLG SPLSALGVEI
160 170 180 190 200
TDSAYVAASM RTMTRMGQPV LDELGDEGFF VKAVHSVGAP LEPGQADVPW
210 220 230 240 250
PCNSTKYISH FPEDREIWSY GSGYGGNALL GKKCYALRIA SVMARDEGWL
260 270 280 290 300
AEHMLVLKLT PPTGAPKYVA AAFPSACGKT NLAMLEPTIS GWTVETIGDD
310 320 330 340 350
IAWMRFGEDG RLYAINPEAG FFGVAPGTGE HTNANAMKTL WGNSVFTNVA
360 370 380 390 400
LTDDGDVWWE GMTEETPAHL TDWKGNDWTP ESGTPAAHPN ARFTTPAAQC
410 420 430 440 450
PIIAPEWEDP RGVPISAILF GGRRATAVPL VTESFDWNHG VFLGANVASE
460 470 480 490 500
KTAAAEGKVG ELRRDPFAML PFCGYNMGDY MGHWVDVAKD KDQSKLPKIY
510 520 530 540 550
YVNWFRKDDA GRFVWPGFGE NGRVLKWIVE RLEGRADGVE TPIGVLPTKE
560 570 580 590 600
SLDTDGLDLA DADLEFLLSV DKEVWREEAA LVPEHLNTFG DHTPAELWDQ

YRALVRRLG
Length:609
Mass (Da):66,917
Last modified:December 1, 2001 - v1
Checksum:i80FF6E00B817875A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939122 Genomic DNA. Translation: CAC40592.1.
RefSeqiNP_629131.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAC40592; CAC40592; CAC40592.
GeneIDi1100420.
KEGGisco:SCO4979.
PATRICi23739800. VBIStrCoe124346_5060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939122 Genomic DNA. Translation: CAC40592.1.
RefSeqiNP_629131.1. NC_003888.3.

3D structure databases

ProteinModelPortaliQ93JL5.
SMRiQ93JL5. Positions 10-609.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO4979.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC40592; CAC40592; CAC40592.
GeneIDi1100420.
KEGGisco:SCO4979.
PATRICi23739800. VBIStrCoe124346_5060.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
InParanoidiQ93JL5.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.
PhylomeDBiQ93JL5.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiPCKG_STRCO
AccessioniPrimary (citable) accession number: Q93JL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: December 1, 2001
Last modified: January 7, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.