Q93IN2 (BCSA_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cellulose synthase catalytic subunit [UDP-forming]
EC=2.4.1.12

Gene names

Name:	bcsA
Ordered Locus Names:	STM3619

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of cellulose synthase. It polymerizes uridine 5'-diphosphate glucose to cellulose, which is produced as an extracellular component for mechanical and chemical protection at the onset of the stationary phase, when the cells exhibit multicellular behavior (rdar morphotype). Coexpression of cellulose and thin aggregative fimbriae leads to a hydrophobic network with tightly packed cells embedded in a highly inert matrix.
Catalytic activity	UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by bis-(3'-5') cyclic diguanylic acid (c-di-GMP) By similarity.
Pathway	Glycan metabolism; bacterial cellulose biosynthesis.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Domain	There are two conserved domains in the globular part of the protein: the N-terminal domain (domain A) contains the conserved DXD motif and is possibly involved in catalysis and substrate binding. The C-terminal domain (domain B) contains the QXXRW motif and is present only in processive glycosyl transferases. It could be involved in the processivity function of the enzyme, possibly required for holding the growing glycan chain in the active site.
Sequence similarities	Belongs to the glycosyltransferase 2 family. Contains 1 PilZ domain.

Ontologies

Keywords
Biological process	Cellulose biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	c-di-GMP
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	UDP-glucose metabolic process Inferred from electronic annotation. Source: InterPro cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulose synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC cyclic-di-GMP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 874	874	Cellulose synthase catalytic subunit [UDP-forming]		PRO_0000059270
Regions
	Transmembrane	30 – 50	21	Helical; Potential
	Transmembrane	151 – 171	21	Helical; Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Transmembrane	230 – 250	21	Helical; Potential
	Transmembrane	525 – 545	21	Helical; Potential
	Transmembrane	547 – 567	21	Helical; Potential
	Transmembrane	592 – 612	21	Helical; Potential
	Transmembrane	634 – 654	21	Helical; Potential
	Transmembrane	668 – 688	21	Helical; Potential
	Transmembrane	833 – 853	21	Helical; Potential
	Domain	694 – 790	97	PilZ
	Region	271 – 364	94	Catalytic subdomain A
	Region	441 – 501	61	Catalytic subdomain B
Sites
	Active site	313	1	Potential
	Active site	457	1	Potential
	Binding site	360	1	Substrate Potential
	Binding site	362	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q93IN2 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 4C9421B58606310A	FASTA	874	100,044
	10 20 30 40 50 60 MSALSRWLLI PPVSARLSER YQGYRRHGAS PFSAALGCLW TILAWIVFPL EHPRWQRIRD 70 80 90 100 110 120 GHKALYPHIN AARPRPLDPA RYLIQTLWLV MISSTKERHE PRWRSFARLK DVRGRYHQWM 130 140 150 160 170 180 DTLPERVRQK TTHLEKEKEL GHLSNGARRF ILGVIVTFSL ILALICITQP FNPLSQFIFL 190 200 210 220 230 240 LLLWGVALLV RRMPGRFSAL MLIVLSLTVS CRYIWWRYTS TLNWDDPVSL VCGLILLFAE 250 260 270 280 290 300 TYAWIVLVLG YFQVVWPLNR QPVPLPKEMS QWPTVDIFVP TYNEDLNVVK NTIYASLGID 310 320 330 340 350 360 WPKDKLNIWI LDDGGRESFR HFARHVGVHY IARTTHEHAK AGNINNALKH AKGEFVAIFD 370 380 390 400 410 420 CDHVPTRSFL QMTMGWFLKE KQLAMMQTPH HFFSPDPFER NLGRFRKTPN EGTLFYGLVQ 430 440 450 460 470 480 DGNDMWDATF FCGSCAVIRR KPLDEIGGIA VETVTEDAHT SLRLHRRGYT SAYMRIPQAA 490 500 510 520 530 540 GLATESLSAH IGQRIRWARG MVQIFRLDNP LFGKGLKLAQ RLCYLNAMFH FLSGIPRLIF 550 560 570 580 590 600 LTAPLAFLLL HAYIIYAPAL MIALFVIPHM VHASLTNSKI QGKYRHSFWS EIYETVLAWY 610 620 630 640 650 660 IAPPTLVALI NPHKGKFNVT AKGGLVEEKY VDWVISRPYI FLVLLNLLGV AAGVWRYYYG 670 680 690 700 710 720 PENETLTVIV SLVWVFYNLV ILGGAVAVSV ESKQVRRAHR VEIAMPGAIA REDGHLFSCT 730 740 750 760 770 780 VHDFSDGGLG IKINGQAQVL EGQKVNLLLK RGQQEYVFPT QVVRVTGNEV GLQLMPLTTK 790 800 810 820 830 840 QHIDFVQCTF ARADTWALWQ DSFPEDKPLE SLLDILKLGF RGYRHLAEFA PPSVKVIFRS 850 860 870 LTALIAWIVS FIPRRPERQA AIQPSDRVMA QAQQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix." Zogaj X., Nimtz M., Rohde M., Bokranz W., Roemling U. Mol. Microbiol. 39:1452-1463(2001) [PubMed: 11260463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 14028 / SGSG 2980 / CDC 6516-60 / NCTC 12023.
[2]	"Genetic analysis of Salmonella enteritidis biofilm formation: critical role of cellulose." Solano C., Garcia B., Valle J., Berasain C., Ghigo J.-M., Gamazo C., Lasa I. Mol. Microbiol. 43:793-808(2002) [PubMed: 11929533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[3]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ315770 Genomic DNA. Translation: CAC44015.1. AE006468 Genomic DNA. Translation: AAL22479.1. AJ315148 Genomic DNA. Translation: CAC86199.1.
RefSeq	NP_462520.1. NC_003197.1.
3D structure databases
ProteinModelPortal	Q93IN2.
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Proteomic databases
PRIDE	Q93IN2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1255142.
GenomeReviews	Gene locus STM3619 in contig AE006468_GR.
KEGG	stm:STM3619.
NMPDR	fig|99287.1.peg.3496.
PATRIC	32386055. VBISalEnt20916_3825.
Phylogenomic databases
HOGENOM	HBG632848.
OMA	CYANAML.
ProtClustDB	PRK11498.
Enzyme and pathway databases
BioCyc	STYP99287:STM3619-MONOMER.
Family and domain databases
InterPro	IPR003919. Cell_synth_A. IPR001173. Glyco_trans_2. IPR009875. PilZ_domain. [Graphical view]
KO	K00694.
Pfam	PF00535. Glycos_transf_2. 1 hit. PF07238. PilZ. 1 hit. [Graphical view]
PRINTS	PR01439. CELLSNTHASEA.
TIGRFAMs	TIGR03030. CelA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BCSA_SALTY

Accession

Primary (citable) accession number: Q93IN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program