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Q93HX3

- NAPA_MAGMG

UniProt

Q93HX3 - NAPA_MAGMG

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Protein

Periplasmic nitrate reductase

Gene
napA
Organism
Magnetospirillum magnetotacticum (Aquaspirillum magnetotacticum)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431Iron-sulfur (4Fe-4S) By similarity
Metal bindingi46 – 461Iron-sulfur (4Fe-4S) By similarity
Metal bindingi50 – 501Iron-sulfur (4Fe-4S) By similarity
Metal bindingi78 – 781Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BRENDAi1.7.1.1. 3153.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
OrganismiMagnetospirillum magnetotacticum (Aquaspirillum magnetotacticum)
Taxonomic identifieri188 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeMagnetospirillum

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Tat-type signal Reviewed predictionAdd
BLAST
Chaini35 – 827793Periplasmic nitrate reductaseUniRule annotationPRO_0000045990Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ93HX3.
SMRiQ93HX3. Positions 36-826.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 92574Fe-4S Mo/W bis-MGD-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93HX3-1 [UniParc]FASTAAdd to Basket

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MSLTRRDFIK ANAVPATAAA AGLATPAIAQ PAKANIRWDK GVCRFCGTGC    50
SVLVGVQDGR VVATQGDPDS PVNRGLNCIK GYFLSKIMYG EDRLTRPLLR 100
MKDGKFDKNG EFQPISWDQA FDIMAEKWKE QLKKPDGVTR VGMFGSGQWT 150
IWEGYAASKL YKAGFRSNNL DPNARHCMAS AVAGFMRTFG IDEPMGCYDD 200
IEQTDAFVLW GSNMAEMHPI LWSRVTDRRL THEGCKVAVL STFEHRSFEL 250
ADIPMVFTPQ TDLAILNYIC HYIISKNAYN KEFIDKHVNF KKGATDIGYG 300
LRPTHALEKD QANAATPDKA DPMTFDEFKA FVAEYTVEKV SKLSGVPADK 350
LEALAKLYAD PKVKVVSFWT MGFNQHTRGT WVNNMIYNVH LLMGKISEPG 400
NSPFSLTGQP SACGTAREVG TFAHRLPADM VVMNDKHREI AERCWKLPAG 450
TINPKIGYHA VLQHRMLKDG KLNAYWVMCT NNMQTAPNMN EEGYPGYRNP 500
ANFIVVSDPY PTVTALAADL ILPTAMWMEK EGAYGNAERR TQFWAPEGQG 550
SGRGRSDLWQ IMEFSKRFKI EEVWPEELIA KKPELRGKTL FEVLYKNGQV 600
DKFPLTELQA GFENDEAKAF GFYPQKGLFE EYASFGRGHA HDLAPFESYH 650
KARGLRWPVV DGKETLWRFR EGYDPYVKAG EGVKFYGKPD GKAWIFALPY 700
APAAESPDKD FDLWLSTGRV LEHWHSGSMT RRVPELHKSV PNAVLYMHPN 750
DAAKRNLRNG DVVKVASRRG EVTTRIDTRG RNKPPEGLVF MPFFDESQLV 800
NKLTLDATCP ISKETDYKKC AVKVSKA 827
Length:827
Mass (Da):92,949
Last modified:December 1, 2001 - v1
Checksum:i5AAC5511C9152335
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB055444 Genomic DNA. Translation: BAB59022.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB055444 Genomic DNA. Translation: BAB59022.1 .

3D structure databases

ProteinModelPortali Q93HX3.
SMRi Q93HX3. Positions 36-826.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 1.7.1.1. 3153.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of periplasmic nitrate reductase gene cluster from magnetotactic bacterium Magnetospirillum magnetotacticum."
    Taoka A., Fukumori Y.
    Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MS-1.

Entry informationi

Entry nameiNAPA_MAGMG
AccessioniPrimary (citable) accession number: Q93HX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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