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Q93HX3

- NAPA_MAGMG

UniProt

Q93HX3 - NAPA_MAGMG

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Magnetospirillum magnetotacticum (Aquaspirillum magnetotacticum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi78 – 781Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BRENDAi1.7.1.1. 3153.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
OrganismiMagnetospirillum magnetotacticum (Aquaspirillum magnetotacticum)
Taxonomic identifieri188 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeMagnetospirillum

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Tat-type signalUniRule annotationAdd
BLAST
Chaini35 – 827793Periplasmic nitrate reductasePRO_0000045990Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ93HX3.
SMRiQ93HX3. Positions 36-826.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 92574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93HX3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLTRRDFIK ANAVPATAAA AGLATPAIAQ PAKANIRWDK GVCRFCGTGC
60 70 80 90 100
SVLVGVQDGR VVATQGDPDS PVNRGLNCIK GYFLSKIMYG EDRLTRPLLR
110 120 130 140 150
MKDGKFDKNG EFQPISWDQA FDIMAEKWKE QLKKPDGVTR VGMFGSGQWT
160 170 180 190 200
IWEGYAASKL YKAGFRSNNL DPNARHCMAS AVAGFMRTFG IDEPMGCYDD
210 220 230 240 250
IEQTDAFVLW GSNMAEMHPI LWSRVTDRRL THEGCKVAVL STFEHRSFEL
260 270 280 290 300
ADIPMVFTPQ TDLAILNYIC HYIISKNAYN KEFIDKHVNF KKGATDIGYG
310 320 330 340 350
LRPTHALEKD QANAATPDKA DPMTFDEFKA FVAEYTVEKV SKLSGVPADK
360 370 380 390 400
LEALAKLYAD PKVKVVSFWT MGFNQHTRGT WVNNMIYNVH LLMGKISEPG
410 420 430 440 450
NSPFSLTGQP SACGTAREVG TFAHRLPADM VVMNDKHREI AERCWKLPAG
460 470 480 490 500
TINPKIGYHA VLQHRMLKDG KLNAYWVMCT NNMQTAPNMN EEGYPGYRNP
510 520 530 540 550
ANFIVVSDPY PTVTALAADL ILPTAMWMEK EGAYGNAERR TQFWAPEGQG
560 570 580 590 600
SGRGRSDLWQ IMEFSKRFKI EEVWPEELIA KKPELRGKTL FEVLYKNGQV
610 620 630 640 650
DKFPLTELQA GFENDEAKAF GFYPQKGLFE EYASFGRGHA HDLAPFESYH
660 670 680 690 700
KARGLRWPVV DGKETLWRFR EGYDPYVKAG EGVKFYGKPD GKAWIFALPY
710 720 730 740 750
APAAESPDKD FDLWLSTGRV LEHWHSGSMT RRVPELHKSV PNAVLYMHPN
760 770 780 790 800
DAAKRNLRNG DVVKVASRRG EVTTRIDTRG RNKPPEGLVF MPFFDESQLV
810 820
NKLTLDATCP ISKETDYKKC AVKVSKA
Length:827
Mass (Da):92,949
Last modified:December 1, 2001 - v1
Checksum:i5AAC5511C9152335
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB055444 Genomic DNA. Translation: BAB59022.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB055444 Genomic DNA. Translation: BAB59022.1 .

3D structure databases

ProteinModelPortali Q93HX3.
SMRi Q93HX3. Positions 36-826.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 1.7.1.1. 3153.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of periplasmic nitrate reductase gene cluster from magnetotactic bacterium Magnetospirillum magnetotacticum."
    Taoka A., Fukumori Y.
    Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MS-1.

Entry informationi

Entry nameiNAPA_MAGMG
AccessioniPrimary (citable) accession number: Q93HX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: December 1, 2001
Last modified: November 26, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3