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Q93HX3 (NAPA_MAGMG) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
OrganismMagnetospirillum magnetotacticum (Aquaspirillum magnetotacticum)
Taxonomic identifier188 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeMagnetospirillum

Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434Tat-type signal Potential
Chain35 – 827793Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000045990

Regions

Domain36 – 92574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding431Iron-sulfur (4Fe-4S) By similarity
Metal binding461Iron-sulfur (4Fe-4S) By similarity
Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding781Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q93HX3 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 5AAC5511C9152335

FASTA82792,949
        10         20         30         40         50         60 
MSLTRRDFIK ANAVPATAAA AGLATPAIAQ PAKANIRWDK GVCRFCGTGC SVLVGVQDGR 

        70         80         90        100        110        120 
VVATQGDPDS PVNRGLNCIK GYFLSKIMYG EDRLTRPLLR MKDGKFDKNG EFQPISWDQA 

       130        140        150        160        170        180 
FDIMAEKWKE QLKKPDGVTR VGMFGSGQWT IWEGYAASKL YKAGFRSNNL DPNARHCMAS 

       190        200        210        220        230        240 
AVAGFMRTFG IDEPMGCYDD IEQTDAFVLW GSNMAEMHPI LWSRVTDRRL THEGCKVAVL 

       250        260        270        280        290        300 
STFEHRSFEL ADIPMVFTPQ TDLAILNYIC HYIISKNAYN KEFIDKHVNF KKGATDIGYG 

       310        320        330        340        350        360 
LRPTHALEKD QANAATPDKA DPMTFDEFKA FVAEYTVEKV SKLSGVPADK LEALAKLYAD 

       370        380        390        400        410        420 
PKVKVVSFWT MGFNQHTRGT WVNNMIYNVH LLMGKISEPG NSPFSLTGQP SACGTAREVG 

       430        440        450        460        470        480 
TFAHRLPADM VVMNDKHREI AERCWKLPAG TINPKIGYHA VLQHRMLKDG KLNAYWVMCT 

       490        500        510        520        530        540 
NNMQTAPNMN EEGYPGYRNP ANFIVVSDPY PTVTALAADL ILPTAMWMEK EGAYGNAERR 

       550        560        570        580        590        600 
TQFWAPEGQG SGRGRSDLWQ IMEFSKRFKI EEVWPEELIA KKPELRGKTL FEVLYKNGQV 

       610        620        630        640        650        660 
DKFPLTELQA GFENDEAKAF GFYPQKGLFE EYASFGRGHA HDLAPFESYH KARGLRWPVV 

       670        680        690        700        710        720 
DGKETLWRFR EGYDPYVKAG EGVKFYGKPD GKAWIFALPY APAAESPDKD FDLWLSTGRV 

       730        740        750        760        770        780 
LEHWHSGSMT RRVPELHKSV PNAVLYMHPN DAAKRNLRNG DVVKVASRRG EVTTRIDTRG 

       790        800        810        820 
RNKPPEGLVF MPFFDESQLV NKLTLDATCP ISKETDYKKC AVKVSKA 

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References

[1]"Cloning and sequencing of periplasmic nitrate reductase gene cluster from magnetotactic bacterium Magnetospirillum magnetotacticum."
Taoka A., Fukumori Y.
Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: MS-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB055444 Genomic DNA. Translation: BAB59022.1.

3D structure databases

ProteinModelPortalQ93HX3.
SMRQ93HX3. Positions 36-826.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA1.7.1.1. 3153.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_MAGMG
AccessionPrimary (citable) accession number: Q93HX3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families