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Q93HX3

- NAPA_MAGMG

UniProt

Q93HX3 - NAPA_MAGMG

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Magnetospirillum magnetotacticum (Aquaspirillum magnetotacticum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi43 – 431Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi78 – 781Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BRENDAi1.7.1.1. 3153.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    OrganismiMagnetospirillum magnetotacticum (Aquaspirillum magnetotacticum)
    Taxonomic identifieri188 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeMagnetospirillum

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3434Tat-type signalUniRule annotationAdd
    BLAST
    Chaini35 – 827793Periplasmic nitrate reductasePRO_0000045990Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ93HX3.
    SMRiQ93HX3. Positions 36-826.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini36 – 92574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q93HX3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLTRRDFIK ANAVPATAAA AGLATPAIAQ PAKANIRWDK GVCRFCGTGC    50
    SVLVGVQDGR VVATQGDPDS PVNRGLNCIK GYFLSKIMYG EDRLTRPLLR 100
    MKDGKFDKNG EFQPISWDQA FDIMAEKWKE QLKKPDGVTR VGMFGSGQWT 150
    IWEGYAASKL YKAGFRSNNL DPNARHCMAS AVAGFMRTFG IDEPMGCYDD 200
    IEQTDAFVLW GSNMAEMHPI LWSRVTDRRL THEGCKVAVL STFEHRSFEL 250
    ADIPMVFTPQ TDLAILNYIC HYIISKNAYN KEFIDKHVNF KKGATDIGYG 300
    LRPTHALEKD QANAATPDKA DPMTFDEFKA FVAEYTVEKV SKLSGVPADK 350
    LEALAKLYAD PKVKVVSFWT MGFNQHTRGT WVNNMIYNVH LLMGKISEPG 400
    NSPFSLTGQP SACGTAREVG TFAHRLPADM VVMNDKHREI AERCWKLPAG 450
    TINPKIGYHA VLQHRMLKDG KLNAYWVMCT NNMQTAPNMN EEGYPGYRNP 500
    ANFIVVSDPY PTVTALAADL ILPTAMWMEK EGAYGNAERR TQFWAPEGQG 550
    SGRGRSDLWQ IMEFSKRFKI EEVWPEELIA KKPELRGKTL FEVLYKNGQV 600
    DKFPLTELQA GFENDEAKAF GFYPQKGLFE EYASFGRGHA HDLAPFESYH 650
    KARGLRWPVV DGKETLWRFR EGYDPYVKAG EGVKFYGKPD GKAWIFALPY 700
    APAAESPDKD FDLWLSTGRV LEHWHSGSMT RRVPELHKSV PNAVLYMHPN 750
    DAAKRNLRNG DVVKVASRRG EVTTRIDTRG RNKPPEGLVF MPFFDESQLV 800
    NKLTLDATCP ISKETDYKKC AVKVSKA 827
    Length:827
    Mass (Da):92,949
    Last modified:December 1, 2001 - v1
    Checksum:i5AAC5511C9152335
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB055444 Genomic DNA. Translation: BAB59022.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB055444 Genomic DNA. Translation: BAB59022.1 .

    3D structure databases

    ProteinModelPortali Q93HX3.
    SMRi Q93HX3. Positions 36-826.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 1.7.1.1. 3153.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of periplasmic nitrate reductase gene cluster from magnetotactic bacterium Magnetospirillum magnetotacticum."
      Taoka A., Fukumori Y.
      Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: MS-1.

    Entry informationi

    Entry nameiNAPA_MAGMG
    AccessioniPrimary (citable) accession number: Q93HX3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3