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Protein

Intracellular endo-alpha-(1->5)-L-arabinanase

Gene

abn-ts

Organism
Geobacillus thermodenitrificans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleavage of endo alpha-(1->5)-L-arabinofuranosyl residues in debranched arabinan.1 Publication

Catalytic activityi

Endohydrolysis of (1->5)-alpha-arabinofuranosidic linkages in (1->5)-arabinans.

Cofactori

Ca2+CuratedNote: Binds 1 Ca2+ ion per subunit.Curated

Temperature dependencei

Optimum temperature is 70 degrees Celsius. The enzyme is stable for 30 minutes at temperatures up to 70 degrees Celsius.1 Publication

Pathwayi: L-arabinan degradation

This protein is involved in the pathway L-arabinan degradation, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway L-arabinan degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei27 – 271Proton acceptorBy similarity
Binding sitei27 – 271SubstrateBy similarity
Binding sitei105 – 1051Substrate; via amide nitrogen
Sitei147 – 1471Important for catalytic activityBy similarity
Active sitei201 – 2011Proton donorBy similarity
Metal bindingi271 – 2711CalciumSequence analysis
Sitei271 – 2711Important for substrate recognitionBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00667.

Protein family/group databases

CAZyiGH43. Glycoside Hydrolase Family 43.

Names & Taxonomyi

Protein namesi
Recommended name:
Intracellular endo-alpha-(1->5)-L-arabinanase (EC:3.2.1.99)
Short name:
ABN
Alternative name(s):
Endo-1,5-alpha-L-arabinanase
Gene namesi
Name:abn-ts
OrganismiGeobacillus thermodenitrificans
Taxonomic identifieri33940 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 1716Missing : No activity is found after 5 minutes at 70 degrees Celsius. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Intracellular endo-alpha-(1->5)-L-arabinanasePRO_0000422130Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

Secondary structure

1
313
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni12 – 143Combined sources
Beta strandi29 – 335Combined sources
Beta strandi36 – 449Combined sources
Beta strandi47 – 6317Combined sources
Helixi71 – 744Combined sources
Beta strandi82 – 9110Combined sources
Beta strandi94 – 1018Combined sources
Beta strandi109 – 1179Combined sources
Beta strandi129 – 1379Combined sources
Beta strandi141 – 1433Combined sources
Beta strandi149 – 1524Combined sources
Beta strandi158 – 1625Combined sources
Beta strandi169 – 1746Combined sources
Turni176 – 1783Combined sources
Beta strandi188 – 1914Combined sources
Beta strandi194 – 1974Combined sources
Beta strandi200 – 2089Combined sources
Beta strandi211 – 2199Combined sources
Helixi224 – 2263Combined sources
Beta strandi230 – 2389Combined sources
Helixi251 – 2533Combined sources
Beta strandi257 – 2604Combined sources
Beta strandi264 – 27714Combined sources
Beta strandi280 – 28910Combined sources
Turni290 – 2945Combined sources
Beta strandi296 – 3027Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WL7X-ray1.90A2-313[»]
ProteinModelPortaliQ93HT9.
SMRiQ93HT9. Positions 2-313.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ93HT9.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni144 – 1474Substrate bindingBy similarity
Regioni164 – 1663Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 43 family.Curated

Family and domain databases

Gene3Di2.115.10.20. 1 hit.
InterProiIPR006710. Glyco_hydro_43.
IPR016840. Glyco_hydro_43_endo_a_Ara-ase.
IPR023296. Glyco_hydro_beta-prop.
[Graphical view]
PANTHERiPTHR22925. PTHR22925. 1 hit.
PfamiPF04616. Glyco_hydro_43. 1 hit.
[Graphical view]
PIRSFiPIRSF026534. Endo_alpha-L-arabinosidase. 1 hit.
SUPFAMiSSF75005. SSF75005. 1 hit.

Sequencei

Sequence statusi: Complete.

Q93HT9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHFHPFGNV NFYEMDWSLK GDLWAHDPVI AKEGSRWYVF HTGSGIQIKT
60 70 80 90 100
SEDGVHWENM GRVFPSLPDW CKQYVPEKDE DHLWAPDICF YNGIYYLYYS
110 120 130 140 150
VSTFGKNTSV IGLATNRTLD PRDPDYEWKD MGPVIHSTAS DNYNAIDPNV
160 170 180 190 200
VFDQEGQPWL SFGSFWSGIQ LIQLDTETMK PAAQAELLTI ASRGEEPNAI
210 220 230 240 250
EAPFIVCRNG YYYLFVSFDF CCRGIESTYK IAVGRSKDIT GPYVDKNGVS
260 270 280 290 300
MMQGGGTILD AGNDRWIGPG HCAVYFSGVS AILVNHAYDA LKNGEPTLQI
310
RPLYWDDEGW PYL
Length:313
Mass (Da):35,457
Last modified:December 1, 2001 - v1
Checksum:i5AA483062433C1DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB061269 Genomic DNA. Translation: BAB64339.1.
PIRiJC7817.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB061269 Genomic DNA. Translation: BAB64339.1.
PIRiJC7817.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WL7X-ray1.90A2-313[»]
ProteinModelPortaliQ93HT9.
SMRiQ93HT9. Positions 2-313.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH43. Glycoside Hydrolase Family 43.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00667.

Miscellaneous databases

EvolutionaryTraceiQ93HT9.

Family and domain databases

Gene3Di2.115.10.20. 1 hit.
InterProiIPR006710. Glyco_hydro_43.
IPR016840. Glyco_hydro_43_endo_a_Ara-ase.
IPR023296. Glyco_hydro_beta-prop.
[Graphical view]
PANTHERiPTHR22925. PTHR22925. 1 hit.
PfamiPF04616. Glyco_hydro_43. 1 hit.
[Graphical view]
PIRSFiPIRSF026534. Endo_alpha-L-arabinosidase. 1 hit.
SUPFAMiSSF75005. SSF75005. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIABN_GEOTD
AccessioniPrimary (citable) accession number: Q93HT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: December 1, 2001
Last modified: October 14, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The N-terminal 16 amino acids plays an important role in the thermostability.1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.