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Protein

Beta-galactosidase III

Gene

beta-galIII

Organism
Bifidobacterium longum subsp. infantis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specific for beta-D-anomer-linked galactoside substrates. Hydrolyzes o-nitrophenyl-beta-D-galactopyranoside (ONPG), chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) and to a lesser extent lactose. Hydrolyzes p-nitrophenyl-beta-D-galacturonide very slightly. Does not hydrolyze maltose, sucrose, raffinose or melibiose. Has some transgalactosylation activity yielding galacto-oligosaccharides (GaOS), including O-beta-D-galactopyranosyl-(1,3)-O-beta-D-galactopyranosyl-(1-4)-D-glucopyranose.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei121 – 1211SubstrateBy similarity
Binding sitei159 – 1591SubstrateBy similarity
Active sitei160 – 1601Proton donorBy similarity
Active sitei318 – 3181NucleophileBy similarity
Binding sitei326 – 3261SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase IIIImported (EC:3.2.1.23)
Short name:
Beta-galBy similarity
Gene namesi
Name:beta-galIIIImported
OrganismiBifidobacterium longum subsp. infantis
Taxonomic identifieri1682 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 691691Beta-galactosidase IIIPRO_0000407685Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ93GI5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni366 – 3694Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q93GI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHRAFKWPQ PLAGNKPRIW YVGDYNPDQW PEEVWDEDVA LMQQAGVNLV
60 70 80 90 100
SVAIFSWAKL EPEEGVYDFD WLDRVIDKLG KAGIAVDLAS GTASPPMWMT
110 120 130 140 150
QAHPEILWVD YRGDVCQPGA RQHWRATSPV FLDYALNLCR KMAEHYKDNP
160 170 180 190 200
YVVSWHVSNE YGCHNRFDYS EDAERAFQKW CEKKYGTIDA VNDAWGTAFW
210 220 230 240 250
AQRMNNFSEI IPPRFIGDGN FMNPGKLLDW KRFSSDALLD FYKAERDALL
260 270 280 290 300
EIAPKPQTTN FMVSAGCTVL DYDKWGHDVD FVSNDHYFSP GEAHFDEMAY
310 320 330 340 350
AACLTDGIAR KNPWFLMEHS TSAVNWRPTN YRLEPGELVR DSLAHLAMGA
360 370 380 390 400
DAICYFQWRQ SKAGAEKWHS AMVPHAGPDS QIFRDVCELG ADLNKLADEG
410 420 430 440 450
LLSTKLVKSK VAVVFDYESQ WATEHTATPT QEVRHWTEPL DWFRALADNG
460 470 480 490 500
LTADVVPVRG PWDEYEAVVL PSLAILSEQT TRRVREYVAN GGKLFVTYYT
510 520 530 540 550
GLVDDRDHVW LGGYPGSIRD VVGVRVEEFA PMGTDAPGTM DHLDLDNGTV
560 570 580 590 600
AHDFADVITS VADTAHVVAS FKADKWTGFD GAPAITVNDF GDGKAAYVGA
610 620 630 640 650
RLGREGLAKS LPALLEELGI ETSAEDDRGE VVRVERADET GENHFVFLFN
660 670 680 690
RTHDVAVVDV EGEPLVASLA QVNESEHTAA IQPNGVLVVK L
Length:691
Mass (Da):77,453
Last modified:December 1, 2001 - v1
Checksum:iDAC10B3E74607C7F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF192266 Genomic DNA. Translation: AAL02053.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF192266 Genomic DNA. Translation: AAL02053.1.

3D structure databases

ProteinModelPortaliQ93GI5.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular and biochemical analysis of two beta-galactosidases from Bifidobacterium infantis HL96."
    Hung M.N., Xia Z., Hu N.T., Lee B.H.
    Appl. Environ. Microbiol. 67:4256-4263(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY.
    Strain: HL96Imported.

Entry informationi

Entry nameiBGAL_BIFLI
AccessioniPrimary (citable) accession number: Q93GI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: December 1, 2001
Last modified: June 24, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.