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Q93GI5

- BGAL_BIFLI

UniProt

Q93GI5 - BGAL_BIFLI

Protein

Beta-galactosidase III

Gene

beta-galIII

Organism
Bifidobacterium longum subsp. infantis
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Specific for beta-D-anomer-linked galactoside substrates. Hydrolyzes o-nitrophenyl-beta-D-galactopyranoside (ONPG), chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) and to a lesser extent lactose. Hydrolyzes p-nitrophenyl-beta-D-galacturonide very slightly. Does not hydrolyze maltose, sucrose, raffinose or melibiose. Has some transgalactosylation activity yielding galacto-oligosaccharides (GaOS), including O-beta-D-galactopyranosyl-(1,3)-O-beta-D-galactopyranosyl-(1-4)-D-glucopyranose.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei121 – 1211SubstrateBy similarity
    Binding sitei159 – 1591SubstrateBy similarity
    Active sitei160 – 1601Proton donorBy similarity
    Active sitei318 – 3181NucleophileBy similarity
    Binding sitei326 – 3261SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. galactose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase IIIImported (EC:3.2.1.23)
    Short name:
    Beta-galBy similarity
    Gene namesi
    Name:beta-galIIIImported
    OrganismiBifidobacterium longum subsp. infantis
    Taxonomic identifieri1682 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 691691Beta-galactosidase IIIPRO_0000407685Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ93GI5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni366 – 3694Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q93GI5-1 [UniParc]FASTAAdd to Basket

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    MEHRAFKWPQ PLAGNKPRIW YVGDYNPDQW PEEVWDEDVA LMQQAGVNLV    50
    SVAIFSWAKL EPEEGVYDFD WLDRVIDKLG KAGIAVDLAS GTASPPMWMT 100
    QAHPEILWVD YRGDVCQPGA RQHWRATSPV FLDYALNLCR KMAEHYKDNP 150
    YVVSWHVSNE YGCHNRFDYS EDAERAFQKW CEKKYGTIDA VNDAWGTAFW 200
    AQRMNNFSEI IPPRFIGDGN FMNPGKLLDW KRFSSDALLD FYKAERDALL 250
    EIAPKPQTTN FMVSAGCTVL DYDKWGHDVD FVSNDHYFSP GEAHFDEMAY 300
    AACLTDGIAR KNPWFLMEHS TSAVNWRPTN YRLEPGELVR DSLAHLAMGA 350
    DAICYFQWRQ SKAGAEKWHS AMVPHAGPDS QIFRDVCELG ADLNKLADEG 400
    LLSTKLVKSK VAVVFDYESQ WATEHTATPT QEVRHWTEPL DWFRALADNG 450
    LTADVVPVRG PWDEYEAVVL PSLAILSEQT TRRVREYVAN GGKLFVTYYT 500
    GLVDDRDHVW LGGYPGSIRD VVGVRVEEFA PMGTDAPGTM DHLDLDNGTV 550
    AHDFADVITS VADTAHVVAS FKADKWTGFD GAPAITVNDF GDGKAAYVGA 600
    RLGREGLAKS LPALLEELGI ETSAEDDRGE VVRVERADET GENHFVFLFN 650
    RTHDVAVVDV EGEPLVASLA QVNESEHTAA IQPNGVLVVK L 691
    Length:691
    Mass (Da):77,453
    Last modified:December 1, 2001 - v1
    Checksum:iDAC10B3E74607C7F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF192266 Genomic DNA. Translation: AAL02053.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF192266 Genomic DNA. Translation: AAL02053.1 .

    3D structure databases

    ProteinModelPortali Q93GI5.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH42. Glycoside Hydrolase Family 42.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProi IPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001084. B-galactosidase. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular and biochemical analysis of two beta-galactosidases from Bifidobacterium infantis HL96."
      Hung M.N., Xia Z., Hu N.T., Lee B.H.
      Appl. Environ. Microbiol. 67:4256-4263(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY.
      Strain: HL96Imported.

    Entry informationi

    Entry nameiBGAL_BIFLI
    AccessioniPrimary (citable) accession number: Q93GI5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3