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Protein

Beta-galactosidase III

Gene

beta-galIII

Organism
Bifidobacterium longum subsp. infantis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specific for beta-D-anomer-linked galactoside substrates. Hydrolyzes o-nitrophenyl-beta-D-galactopyranoside (ONPG), chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) and to a lesser extent lactose. Hydrolyzes p-nitrophenyl-beta-D-galacturonide very slightly. Does not hydrolyze maltose, sucrose, raffinose or melibiose. Has some transgalactosylation activity yielding galacto-oligosaccharides (GaOS), including O-beta-D-galactopyranosyl-(1,3)-O-beta-D-galactopyranosyl-1-4-D-glucopyranose.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei121SubstrateBy similarity1
Binding sitei159SubstrateBy similarity1
Active sitei160Proton donorBy similarity1
Active sitei318NucleophileBy similarity1
Binding sitei326SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase IIIImported (EC:3.2.1.23)
Short name:
Beta-galBy similarity
Gene namesi
Name:beta-galIIIImported
OrganismiBifidobacterium longum subsp. infantis
Taxonomic identifieri1682 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004076851 – 691Beta-galactosidase IIIAdd BLAST691

Structurei

3D structure databases

ProteinModelPortaliQ93GI5.
SMRiQ93GI5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni366 – 369Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Sequence analysis

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.40.50.880. 1 hit.
InterProiView protein in InterPro
IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
PANTHERiPTHR36447. PTHR36447. 1 hit.
PfamiView protein in Pfam
PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q93GI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHRAFKWPQ PLAGNKPRIW YVGDYNPDQW PEEVWDEDVA LMQQAGVNLV
60 70 80 90 100
SVAIFSWAKL EPEEGVYDFD WLDRVIDKLG KAGIAVDLAS GTASPPMWMT
110 120 130 140 150
QAHPEILWVD YRGDVCQPGA RQHWRATSPV FLDYALNLCR KMAEHYKDNP
160 170 180 190 200
YVVSWHVSNE YGCHNRFDYS EDAERAFQKW CEKKYGTIDA VNDAWGTAFW
210 220 230 240 250
AQRMNNFSEI IPPRFIGDGN FMNPGKLLDW KRFSSDALLD FYKAERDALL
260 270 280 290 300
EIAPKPQTTN FMVSAGCTVL DYDKWGHDVD FVSNDHYFSP GEAHFDEMAY
310 320 330 340 350
AACLTDGIAR KNPWFLMEHS TSAVNWRPTN YRLEPGELVR DSLAHLAMGA
360 370 380 390 400
DAICYFQWRQ SKAGAEKWHS AMVPHAGPDS QIFRDVCELG ADLNKLADEG
410 420 430 440 450
LLSTKLVKSK VAVVFDYESQ WATEHTATPT QEVRHWTEPL DWFRALADNG
460 470 480 490 500
LTADVVPVRG PWDEYEAVVL PSLAILSEQT TRRVREYVAN GGKLFVTYYT
510 520 530 540 550
GLVDDRDHVW LGGYPGSIRD VVGVRVEEFA PMGTDAPGTM DHLDLDNGTV
560 570 580 590 600
AHDFADVITS VADTAHVVAS FKADKWTGFD GAPAITVNDF GDGKAAYVGA
610 620 630 640 650
RLGREGLAKS LPALLEELGI ETSAEDDRGE VVRVERADET GENHFVFLFN
660 670 680 690
RTHDVAVVDV EGEPLVASLA QVNESEHTAA IQPNGVLVVK L
Length:691
Mass (Da):77,453
Last modified:December 1, 2001 - v1
Checksum:iDAC10B3E74607C7F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF192266 Genomic DNA. Translation: AAL02053.1.

Similar proteinsi

Entry informationi

Entry nameiBGAL_BIFLI
AccessioniPrimary (citable) accession number: Q93GI5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: December 1, 2001
Last modified: February 28, 2018
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program