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Q93GI5 (BGAL_BIFLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase III

Short name=Beta-gal
EC=3.2.1.23
Gene names
Name:beta-galIII
OrganismBifidobacterium longum subsp. infantis
Taxonomic identifier1682 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Protein attributes

Sequence length691 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specific for beta-D-anomer-linked galactoside substrates. Hydrolyzes o-nitrophenyl-beta-D-galactopyranoside (ONPG), chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) and to a lesser extent lactose. Hydrolyzes p-nitrophenyl-beta-D-galacturonide very slightly. Does not hydrolyze maltose, sucrose, raffinose or melibiose. Has some transgalactosylation activity yielding galacto-oligosaccharides (GaOS), including O-beta-D-galactopyranosyl-(1,3)-O-beta-D-galactopyranosyl-(1-4)-D-glucopyranose. Ref.1

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 42 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processgalactose metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 691691Beta-galactosidase III
PRO_0000407685

Regions

Region366 – 3694Substrate binding

Sites

Active site1601Proton donor By similarity
Active site3181Nucleophile By similarity
Binding site1211Substrate By similarity
Binding site1591Substrate By similarity
Binding site3261Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q93GI5 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: DAC10B3E74607C7F

FASTA69177,453
        10         20         30         40         50         60 
MEHRAFKWPQ PLAGNKPRIW YVGDYNPDQW PEEVWDEDVA LMQQAGVNLV SVAIFSWAKL 

        70         80         90        100        110        120 
EPEEGVYDFD WLDRVIDKLG KAGIAVDLAS GTASPPMWMT QAHPEILWVD YRGDVCQPGA 

       130        140        150        160        170        180 
RQHWRATSPV FLDYALNLCR KMAEHYKDNP YVVSWHVSNE YGCHNRFDYS EDAERAFQKW 

       190        200        210        220        230        240 
CEKKYGTIDA VNDAWGTAFW AQRMNNFSEI IPPRFIGDGN FMNPGKLLDW KRFSSDALLD 

       250        260        270        280        290        300 
FYKAERDALL EIAPKPQTTN FMVSAGCTVL DYDKWGHDVD FVSNDHYFSP GEAHFDEMAY 

       310        320        330        340        350        360 
AACLTDGIAR KNPWFLMEHS TSAVNWRPTN YRLEPGELVR DSLAHLAMGA DAICYFQWRQ 

       370        380        390        400        410        420 
SKAGAEKWHS AMVPHAGPDS QIFRDVCELG ADLNKLADEG LLSTKLVKSK VAVVFDYESQ 

       430        440        450        460        470        480 
WATEHTATPT QEVRHWTEPL DWFRALADNG LTADVVPVRG PWDEYEAVVL PSLAILSEQT 

       490        500        510        520        530        540 
TRRVREYVAN GGKLFVTYYT GLVDDRDHVW LGGYPGSIRD VVGVRVEEFA PMGTDAPGTM 

       550        560        570        580        590        600 
DHLDLDNGTV AHDFADVITS VADTAHVVAS FKADKWTGFD GAPAITVNDF GDGKAAYVGA 

       610        620        630        640        650        660 
RLGREGLAKS LPALLEELGI ETSAEDDRGE VVRVERADET GENHFVFLFN RTHDVAVVDV 

       670        680        690 
EGEPLVASLA QVNESEHTAA IQPNGVLVVK L 

« Hide

References

[1]"Molecular and biochemical analysis of two beta-galactosidases from Bifidobacterium infantis HL96."
Hung M.N., Xia Z., Hu N.T., Lee B.H.
Appl. Environ. Microbiol. 67:4256-4263(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY.
Strain: HL96.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF192266 Genomic DNA. Translation: AAL02053.1.

3D structure databases

ProteinModelPortalQ93GI5.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFPIRSF001084. B-galactosidase. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGAL_BIFLI
AccessionPrimary (citable) accession number: Q93GI5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: December 1, 2001
Last modified: June 11, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries