Q93GI5 (BGAL_BIFLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase III
Short name=Beta-gal
EC=3.2.1.23

Gene names

Name:

beta-galIII

Organism

Bifidobacterium longum subsp. infantis

Taxonomic identifier

1682 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium › Bifidobacterium longum

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specific for beta-D-anomer-linked galactoside substrates. Hydrolyzes o-nitrophenyl-beta-D-galactopyranoside (ONPG), chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) and to a lesser extent lactose. Hydrolyzes p-nitrophenyl-beta-D-galacturonide very slightly. Does not hydrolyze maltose, sucrose, raffinose or melibiose. Has some transgalactosylation activity yielding galacto-oligosaccharides (GaOS), including O-beta-D-galactopyranosyl-(1,3)-O-beta-D-galactopyranosyl-(1-4)-D-glucopyranose. Ref.1
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 42 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	galactose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	beta-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 691	691	Beta-galactosidase III		PRO_0000407685
Regions
	Region	366 – 369	4	Substrate binding
Sites
	Active site	160	1	Proton donor By similarity
	Active site	318	1	Nucleophile By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	159	1	Substrate By similarity
	Binding site	326	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q93GI5 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: DAC10B3E74607C7F	FASTA	691	77,453
	10 20 30 40 50 60 MEHRAFKWPQ PLAGNKPRIW YVGDYNPDQW PEEVWDEDVA LMQQAGVNLV SVAIFSWAKL 70 80 90 100 110 120 EPEEGVYDFD WLDRVIDKLG KAGIAVDLAS GTASPPMWMT QAHPEILWVD YRGDVCQPGA 130 140 150 160 170 180 RQHWRATSPV FLDYALNLCR KMAEHYKDNP YVVSWHVSNE YGCHNRFDYS EDAERAFQKW 190 200 210 220 230 240 CEKKYGTIDA VNDAWGTAFW AQRMNNFSEI IPPRFIGDGN FMNPGKLLDW KRFSSDALLD 250 260 270 280 290 300 FYKAERDALL EIAPKPQTTN FMVSAGCTVL DYDKWGHDVD FVSNDHYFSP GEAHFDEMAY 310 320 330 340 350 360 AACLTDGIAR KNPWFLMEHS TSAVNWRPTN YRLEPGELVR DSLAHLAMGA DAICYFQWRQ 370 380 390 400 410 420 SKAGAEKWHS AMVPHAGPDS QIFRDVCELG ADLNKLADEG LLSTKLVKSK VAVVFDYESQ 430 440 450 460 470 480 WATEHTATPT QEVRHWTEPL DWFRALADNG LTADVVPVRG PWDEYEAVVL PSLAILSEQT 490 500 510 520 530 540 TRRVREYVAN GGKLFVTYYT GLVDDRDHVW LGGYPGSIRD VVGVRVEEFA PMGTDAPGTM 550 560 570 580 590 600 DHLDLDNGTV AHDFADVITS VADTAHVVAS FKADKWTGFD GAPAITVNDF GDGKAAYVGA 610 620 630 640 650 660 RLGREGLAKS LPALLEELGI ETSAEDDRGE VVRVERADET GENHFVFLFN RTHDVAVVDV 670 680 690 EGEPLVASLA QVNESEHTAA IQPNGVLVVK L

References

[1]

"Molecular and biochemical analysis of two beta-galactosidases from Bifidobacterium infantis HL96." Hung M.N., Xia Z., Hu N.T., Lee B.H. Appl. Environ. Microbiol. 67:4256-4263(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY. Strain: HL96.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF192266 Genomic DNA. Translation: AAL02053.1.
3D structure databases
ProteinModelPortal	Q93GI5.
ModBase	Search...
Protein family/group databases
CAZy	GH42. Glycoside Hydrolase Family 42.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR013739. Beta_galactosidase_C. IPR013738. Beta_galactosidase_Trimer. IPR003476. Glyco_hydro_42. IPR013529. Glyco_hydro_42_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF02449. Glyco_hydro_42. 1 hit. PF08533. Glyco_hydro_42C. 1 hit. PF08532. Glyco_hydro_42M. 1 hit. [Graphical view]
PIRSF	PIRSF001084. B-galactosidase. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BGAL_BIFLI

Accession

Primary (citable) accession number: Q93GI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 3, 2011
Last sequence update:	December 1, 2001
Last modified:	May 29, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program