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Q93GI5

- BGAL_BIFLI

UniProt

Q93GI5 - BGAL_BIFLI

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Protein
Beta-galactosidase III
Gene
beta-galIII
Organism
Bifidobacterium longum subsp. infantis
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Specific for beta-D-anomer-linked galactoside substrates. Hydrolyzes o-nitrophenyl-beta-D-galactopyranoside (ONPG), chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) and to a lesser extent lactose. Hydrolyzes p-nitrophenyl-beta-D-galacturonide very slightly. Does not hydrolyze maltose, sucrose, raffinose or melibiose. Has some transgalactosylation activity yielding galacto-oligosaccharides (GaOS), including O-beta-D-galactopyranosyl-(1,3)-O-beta-D-galactopyranosyl-(1-4)-D-glucopyranose.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei121 – 1211Substrate By similarity
Binding sitei159 – 1591Substrate By similarity
Active sitei160 – 1601Proton donor By similarity
Active sitei318 – 3181Nucleophile By similarity
Binding sitei326 – 3261Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase III (EC:3.2.1.23)
Short name:
Beta-gal
Gene namesi
Name:beta-galIII
OrganismiBifidobacterium longum subsp. infantis
Taxonomic identifieri1682 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 691691Beta-galactosidase III
PRO_0000407685Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ93GI5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni366 – 3694Substrate binding

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q93GI5-1 [UniParc]FASTAAdd to Basket

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MEHRAFKWPQ PLAGNKPRIW YVGDYNPDQW PEEVWDEDVA LMQQAGVNLV    50
SVAIFSWAKL EPEEGVYDFD WLDRVIDKLG KAGIAVDLAS GTASPPMWMT 100
QAHPEILWVD YRGDVCQPGA RQHWRATSPV FLDYALNLCR KMAEHYKDNP 150
YVVSWHVSNE YGCHNRFDYS EDAERAFQKW CEKKYGTIDA VNDAWGTAFW 200
AQRMNNFSEI IPPRFIGDGN FMNPGKLLDW KRFSSDALLD FYKAERDALL 250
EIAPKPQTTN FMVSAGCTVL DYDKWGHDVD FVSNDHYFSP GEAHFDEMAY 300
AACLTDGIAR KNPWFLMEHS TSAVNWRPTN YRLEPGELVR DSLAHLAMGA 350
DAICYFQWRQ SKAGAEKWHS AMVPHAGPDS QIFRDVCELG ADLNKLADEG 400
LLSTKLVKSK VAVVFDYESQ WATEHTATPT QEVRHWTEPL DWFRALADNG 450
LTADVVPVRG PWDEYEAVVL PSLAILSEQT TRRVREYVAN GGKLFVTYYT 500
GLVDDRDHVW LGGYPGSIRD VVGVRVEEFA PMGTDAPGTM DHLDLDNGTV 550
AHDFADVITS VADTAHVVAS FKADKWTGFD GAPAITVNDF GDGKAAYVGA 600
RLGREGLAKS LPALLEELGI ETSAEDDRGE VVRVERADET GENHFVFLFN 650
RTHDVAVVDV EGEPLVASLA QVNESEHTAA IQPNGVLVVK L 691
Length:691
Mass (Da):77,453
Last modified:December 1, 2001 - v1
Checksum:iDAC10B3E74607C7F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF192266 Genomic DNA. Translation: AAL02053.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF192266 Genomic DNA. Translation: AAL02053.1 .

3D structure databases

ProteinModelPortali Q93GI5.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular and biochemical analysis of two beta-galactosidases from Bifidobacterium infantis HL96."
    Hung M.N., Xia Z., Hu N.T., Lee B.H.
    Appl. Environ. Microbiol. 67:4256-4263(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY.
    Strain: HL96.

Entry informationi

Entry nameiBGAL_BIFLI
AccessioniPrimary (citable) accession number: Q93GI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: December 1, 2001
Last modified: June 11, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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