Reviewed, UniProtKB/Swiss-Prot Q93AS1 (HPPA2_RHILT)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump 2
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase 2
      Short name=H(+)-PPase 2
    Membrane-bound proton-translocating pyrophosphatase 2

Gene names

Name:

hppA2

Organism

Rhizobium leguminosarum bv. trifolii

Taxonomic identifier

386 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	215 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01130
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›215	›215	Pyrophosphate-energized proton pump 2 HAMAP MF_01130		PRO_0000217026
Regions
	Transmembrane	13 – 35	23	Potential
	Transmembrane	55 – 77	23	Potential
	Transmembrane	89 – 111	23	Potential
	Transmembrane	133 – 155	23	Potential
	Transmembrane	162 – 184	23	Potential
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	215	1

Sequences

	Sequence		Length	Mass (Da)
	Q93AS1-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 9B8D950E65B48318	FASTA	215	21,711
	10 20 30 40 50 60 AAIFFAGTPI LESAMVYPLA ICGACILTSI AGTFFVKLGT NNSIMGALYK GLIATGVFSV 70 80 90 100 110 120 AGLAVATYAT VGWGTIGTVA GMEITGTNLF FCGLVGLVVT ALIVVITEYY TGTNKRPVNS 130 140 150 160 170 180 IAQASVTGHG TNVIQGLAVS LESTALPAIV IVGGIIGTYQ LGGLFGTGIA VTAMLGLAGM 190 200 210 IVALDAFGPV TDNAGGIAEM AGLDPDVRKR PTRWM

References

[1]

"High prevalence of the H+-proton-pumping inorganic pyrophosphatase gene in alpha proteobacteria and evidence of lateral transfer in its phylogeny." Jumas-Bilak E., Michaux-Charachon S., Teyssier C. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 14480 / 3D1k22a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF417516 Genomic DNA. Translation: AAL14975.1.
3D structure databases
ModBase	Search...
Family and domain databases
HAMAP	MF_01130. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HPPA2_RHILT

Accession

Primary (citable) accession number: Q93AS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	December 1, 2001
Last modified:	October 13, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)