Reviewed, UniProtKB/Swiss-Prot Q93AR9 (HPPA2_MYCDI)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump 2
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase 2
      Short name=H(+)-PPase 2
    Membrane-bound proton-translocating pyrophosphatase 2

Gene names

Name:

hppA2

Organism

Mycoplana dimorpha

Taxonomic identifier

28320 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Mycoplana

Protein attributes

Sequence length	208 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity. HAMAP MF_01130
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01130
Cofactor	Magnesium By similarity. HAMAP MF_01130
Subunit structure	Homodimer Potential. HAMAP MF_01130
Subcellular location	Cell membrane; Multi-pass membrane protein Probable HAMAP MF_01130.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›208	›208	Pyrophosphate-energized proton pump 2 HAMAP MF_01130		PRO_0000217020
Regions
	Transmembrane	20 – 42	23	Potential
	Transmembrane	55 – 77	23	Potential
	Transmembrane	87 – 109	23	Potential
	Transmembrane	164 – 186	23	Potential
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	208	1

Sequences

	Sequence		Length	Mass (Da)
	Q93AR9-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 5A5345C538A8950E	FASTA	208	20,704
	10 20 30 40 50 60 MVLAAIFFGG TPILATAMAY PLAICAACVI TSIIGTFFVK LGTNNSIMGA LYKGLIVTGA 70 80 90 100 110 120 LSILGLGAAT SFTIGWGSIG TVGGIEVRGG NLFVCGLIGL VVTALIVVIT EYYTGTNKRP 130 140 150 160 170 180 VNSIAQASVT GHGTNVIQGL AVSLESTALP AIVIVGGIIA TYQLAGLFGT AIAVTAMLGL 190 200 AGMIVALDAF GPVTDNAGGI AEMSHLSP

References

[1]

"High prevalence of the H+-proton-pumping inorganic pyrophosphatase gene in alpha proteobacteria and evidence of lateral transfer in its phylogeny." Jumas-Bilak E., Michaux-Charachon S., Teyssier C. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 4279 / DSM 7138 / IFO 13291 / NCIMB 9439.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF417518 Genomic DNA. Translation: AAL18698.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.6.1.1. 66799.
Family and domain databases
HAMAP	MF_01130. H_PPase_type2. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HPPA2_MYCDI

Accession

Primary (citable) accession number: Q93AR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	December 1, 2001
Last modified:	January 19, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)