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Protein

Platelet binding protein GspB

Gene

gspB

Organism
Streptococcus gordonii
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in virulence and host-pathogen interactions. Mediates binding to human platelets via interaction with the human cell surface glycoprotein GP1BA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei443Important for interaction with host glycoprotein and virulence1
Sitei484Important for interaction with host glycoprotein and virulence1
Sitei485Important for interaction with host glycoprotein and virulence1

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet binding protein GspB
Alternative name(s):
Serine-rich adhesin for platelets
Serine-rich repeat protein GspB
Gene namesi
Name:gspB
OrganismiStreptococcus gordonii
Taxonomic identifieri1302 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

Loss of adherence to human platelet cells.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi443Y → F: Strongly reduced interaction with GP1BA carbohydrate chains. 1 Publication1
Mutagenesisi484R → E: Strongly reduced interaction with GP1BA carbohydrate chains. Strongly reduced platelet binding. 1 Publication1
Mutagenesisi485Y → F: Strongly reduced interaction with GP1BA carbohydrate chains. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 85Sequence analysisAdd BLAST85
ChainiPRO_000041419286 – 3041Platelet binding protein GspBAdd BLAST2956
PropeptideiPRO_00004141933042 – 3072Removed by sortaseSequence analysisAdd BLAST31

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3041Pentaglycyl murein peptidoglycan amidated threonineSequence analysis1

Post-translational modificationi

Proteolytically cleaved by a metalloprotease.By similarity
Both SSR1 and SSR2 domains are glycosylated. A truncated derivative (about residues 1-2062) contains 105 nmol per nmol of protein, suggesting at least 10% of the apparent molecular weight is due to carbohydrates. Glucose and N-acetylglucosamine are present in a ratio of 30:73 residues per truncated polypeptide, as well as minor amounts of galactose and N-acetylgalactosamine. Glycosylation occurs intracellularly in the Ser-rich regions SSR1 and SSR2. Glycosylation of SSR2 domain may be required to prevent aggregation of GspB.2 Publications

Keywords - PTMi

Glycoprotein, Peptidoglycan-anchor

Interactioni

Subunit structurei

Both SSR domains in the unglycosylated protein bind to Asp2 and Asp3; glycosylated protein binds less well. Interacts with the human cell surface glycoprotein GP1BA.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
asp2Q9AET84EBI-6414561,EBI-6414583
asp3Q9AET74EBI-6414561,EBI-6414568

Protein-protein interaction databases

IntActiQ939N5. 2 interactors.

Structurei

Secondary structure

13072
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi247 – 250Combined sources4
Beta strandi253 – 257Combined sources5
Beta strandi263 – 272Combined sources10
Turni273 – 275Combined sources3
Beta strandi277 – 284Combined sources8
Beta strandi291 – 299Combined sources9
Beta strandi303 – 310Combined sources8
Beta strandi316 – 319Combined sources4
Beta strandi328 – 333Combined sources6
Beta strandi338 – 346Combined sources9
Beta strandi351 – 364Combined sources14
Turni368 – 370Combined sources3
Helixi375 – 377Combined sources3
Turni379 – 382Combined sources4
Beta strandi386 – 395Combined sources10
Beta strandi404 – 406Combined sources3
Beta strandi409 – 414Combined sources6
Beta strandi418 – 426Combined sources9
Helixi434 – 436Combined sources3
Beta strandi438 – 440Combined sources3
Beta strandi442 – 446Combined sources5
Beta strandi455 – 461Combined sources7
Beta strandi464 – 472Combined sources9
Beta strandi475 – 477Combined sources3
Beta strandi480 – 487Combined sources8
Beta strandi495 – 497Combined sources3
Helixi499 – 507Combined sources9
Beta strandi515 – 521Combined sources7
Beta strandi531 – 534Combined sources4
Helixi541 – 554Combined sources14
Helixi560 – 562Combined sources3
Helixi564 – 566Combined sources3
Helixi568 – 571Combined sources4
Beta strandi572 – 574Combined sources3
Beta strandi580 – 584Combined sources5
Beta strandi589 – 592Combined sources4
Turni595 – 597Combined sources3
Beta strandi598 – 601Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QC5X-ray1.40X245-604[»]
3QC6X-ray1.90X245-604[»]
5IUCX-ray1.25A/B399-521[»]
SMRiQ939N5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni123 – 236Ser-rich 1 (SSR1)Add BLAST114
Regioni237 – 3027Basic region (BR)Add BLAST2791
Regioni604 – 3028Ser-rich 2 (SSR2)Add BLAST2425

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi3038 – 3042LPXTG sorting signalSequence analysis5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi123 – 236Ser-richAdd BLAST114
Compositional biasi604 – 3028Ser-richAdd BLAST2425
Compositional biasi669 – 2963Ala-richAdd BLAST2295

Domaini

Has a short and long Ser-rich region with a basic region between them. The SRR domains themselves are comprised of inexact repeats of SASESASTSASV. SSR1 has 6 repeats, SSR2 has 200. The Ser-rich regions are both glycosylated.

Sequence similaritiesi

Belongs to the SraP family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR019948. Gram-positive_anchor.
IPR022263. KxYKxGKxW.
IPR026465. Ser_adhes_glycop.
[Graphical view]
PfamiPF00746. Gram_pos_anchor. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03715. KxYKxGKxW. 1 hit.
TIGR04224. ser_adhes_Nterm. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q939N5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFFKRQKGQY HEVERVTRFK LIKSGKHWLR AATSQFGLLR LMKGSDVSST
60 70 80 90 100
EVKVVEEQSV EKSGLNYLKG IIATGAVLGG AVVTSSSVYA EEEQAHEKVI
110 120 130 140 150
DTRDVLATRG EAVLSEEAAT TLSSTEANPV ESLSDTLSAS ESTSASSSVS
160 170 180 190 200
TSISVSESFS VSGSLSYSTS LSQSVSASAS ASESLSVSSS ASDSVSASTS
210 220 230 240 250
TSASASQSVS ASQKSTISTS ESTRSESSQQ STEASSQTGR RRTRRAVTES
260 270 280 290 300
APNVEYHDVK GDMIQSVTTS FDDTSRLLTW TINLTPRQVK SNLGALVSIS
310 320 330 340 350
GNQETRTVTI NGKNAANGGV YNSGGAWNLY TGESVNNNVL RITTQVNDTG
360 370 380 390 400
GEVKLGLRLV TSDKKITKTN LPLEFSQVAA TTNGSWDKAG YNTTIVEKDT
410 420 430 440 450
ERPVVNVPSE ITVYRGESFE YFATVTDNSN AFDLAKTVVR WLYNNQPGRG
460 470 480 490 500
TEWLQYSVTQ VGNQLKVRIF GNVPIDTTIG DYTRYVVATD AAGNVNATQT
510 520 530 540 550
EMGNAAVDKT SVNGQFKLII RFRIKTPENT VFVNNPNQLT EVEKNLVREA
560 570 580 590 600
VKKSNPDLRA QDVLNSNYVT GITVSNNGTT TITYRDGRKD IIDGSKFIDT
610 620 630 640 650
RAGSISKSQS TSNSISVSLS KSESASASLV TSKLNSISSS ASVSASTSIS
660 670 680 690 700
TSGSVSASES ASTSSSVSAS ESASTSASVS ASESASTSAS VSASTSASTS
710 720 730 740 750
ASVSASTSAS TSASTSASKS ASTSASVSAS TSASTSASVS ASESASTSAS
760 770 780 790 800
VSASTSASTS ASVSASTSAS TSASVSASES ASTSASVSAS TSASTSASVS
810 820 830 840 850
ASESASTSAS VSASTSASTS ASVSASASAS TSASVSASTS ASTSASVSAS
860 870 880 890 900
ASASTSASVS ASTSASTSAS VSASESASTS ASVSASESAS TSASVSASES
910 920 930 940 950
ASTSASVSAS ESASTSASVS ASTSASTSAS VSASESASTS ASVSASESAS
960 970 980 990 1000
TSASVSASES ASTSASVSAS ESASTSASVS ASTSASTSAS VSASTSASTS
1010 1020 1030 1040 1050
ASVSASTSAS TSASVSASTS ASTSASVSAS ESASTSASVS ASESASTSAS
1060 1070 1080 1090 1100
VSASTSASTS ASVSASESAS TSASVSASES ASTSASESAS ESASTSASVS
1110 1120 1130 1140 1150
ASESASTSAS VSASESSSTS ASVSASESSS TSASVSASES ASTSASVSAS
1160 1170 1180 1190 1200
ESASTSASES ASESASTSAS VSASESASTS ASVSASESAS TSASVSASES
1210 1220 1230 1240 1250
VSTSASVSAS ESASTSASVS ASESASTSAS ESASESASTS ASVSASESAS
1260 1270 1280 1290 1300
TSASVSASES ASTSASVSAS TSASTSASVS ASESASTSAS VSASESASTS
1310 1320 1330 1340 1350
ASVSASESAS TSASVSASES VSTSASVSAS ESASTSASVS ASESASTSAS
1360 1370 1380 1390 1400
ESASESASTS ASVSASESAS TSASVSASES ASTSASVSAS TSASTSASVS
1410 1420 1430 1440 1450
ASESASTSAS VSASESASTS ASVSASESAS TSASVSASTS ASTSASVSAS
1460 1470 1480 1490 1500
ESASTSTSVS TSTSASTSAS VSASESASTS ASVSASESAS TSASVSASTS
1510 1520 1530 1540 1550
ASTSASVSAS ESASTSASVS ASESASTSAS VSASESASTS ASVSASESAS
1560 1570 1580 1590 1600
TSASVSASTS ASTSASVSAS ESASTSASVS ASESASTSAS VSASESASTS
1610 1620 1630 1640 1650
ASVSASESAS TSASVSASES ASTSASVSAS ESASTSASVS ASESASTSAS
1660 1670 1680 1690 1700
VSASESASTS ASVSASESAS TSASVSASES ASTSASVSAS ESASTSASVS
1710 1720 1730 1740 1750
ASESASTSAS VSASESASTS ASVSASESAS TSASVSASES ASTSASVSAS
1760 1770 1780 1790 1800
ESASTSASVS ASESASTSAS VSASESASTS ASVSASESAS TSASVSASES
1810 1820 1830 1840 1850
ASTSASVSAS ESASTSASVS ASESASTSAS VSASESASTS ASVSASESAS
1860 1870 1880 1890 1900
TSASVSASES ASTSASVSAS TSTSTSASVS ASESASTSAS VSASESASTS
1910 1920 1930 1940 1950
ASVSASESAS TSASVSASES ASTSASVSAS ESASTSASVS ASESASTSAS
1960 1970 1980 1990 2000
VSASESASTS ASVSASESAS TSASVSASES ASTSASVSAS TSASTSASVS
2010 2020 2030 2040 2050
ASESASTSAS VSASESASTS ASVSASESAS TSASVSASES ASTSASVSAS
2060 2070 2080 2090 2100
ESASTSASVS ASESASTSAS VSASESASTS ASVSASESAS TSASVSASKS
2110 2120 2130 2140 2150
ASTSESASTS ASVSASESAS TSASVSASES ASTSASVSAS ESVSTSASVS
2160 2170 2180 2190 2200
ASDSASISAS VLASESASTS ASVSASESAS TSASVSASES ASTSASVSAS
2210 2220 2230 2240 2250
ESASTSSSVS ASESASTSAS VSASESASTS ASVSASTSAS TSASVSASES
2260 2270 2280 2290 2300
ASTSASVSAS ESASTSASVS ASESASTSAS VSASESASTS ASVSASESAS
2310 2320 2330 2340 2350
TSASVSASES ASTSASVSAS ESASTSASVS ASTSASTSAS VSASESASTS
2360 2370 2380 2390 2400
ASVSSSESAS TSASVSASES ASTSASVSAS ESASTSASVS ASESASTSAS
2410 2420 2430 2440 2450
VSASESASTS ASVSASTSAS TSASVSASES ASTSASVSAS ESASTSASVS
2460 2470 2480 2490 2500
ASESASTSAS VSASESASTS ASVSASTSAS TSASVSASES ASTSASVSAS
2510 2520 2530 2540 2550
ESASTSASVS ASTSASTSAS VSASESASTS ASVSASESAS TSASVSASES
2560 2570 2580 2590 2600
ASTSASVSAS ESASTSASVS ASESASTSAS VSASESASTS ASVSASESAS
2610 2620 2630 2640 2650
TSASVSASMS ASTSASVSVS ESTSTSASVS ANESASTSAS VSASESASTS
2660 2670 2680 2690 2700
ASVSASESAS TSASVSASES ASTSASVSAS ESASTSASVS ASESASTSAS
2710 2720 2730 2740 2750
VSASESASTS ASVSASESAS TSASVSASES ASTSASVSAS ESASTSASVS
2760 2770 2780 2790 2800
ASTSASTSAS VSANESASTS ASVSASESAS TSASVSASES ASTSASVSAS
2810 2820 2830 2840 2850
ESASTSASVS ASESASTSAS VSASTSASTS ASVSANESAS TSASVSASES
2860 2870 2880 2890 2900
ASTSASVSAS ESASTSASVS ASESASTSAS VSASESASTS ASVSASTSAS
2910 2920 2930 2940 2950
TSASVSASES ASTSASASAS ESASTSASVS ASESASTSAS VSASESASTS
2960 2970 2980 2990 3000
ASVSASESAS TNASVSVSES MSVSESLSLS ISTSVLHSQL NDIYESELYS
3010 3020 3030 3040 3050
LSLSESLSAS QSLSQSLSES QSSSASQSMH DRISKGQLPR TGESENKASI
3060 3070
LALGLGALGL AFKKRKKNES ED
Length:3,072
Mass (Da):285,769
Last modified:December 1, 2001 - v1
Checksum:i0B148372697CF7F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY028381 Genomic DNA. Translation: AAL13053.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY028381 Genomic DNA. Translation: AAL13053.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QC5X-ray1.40X245-604[»]
3QC6X-ray1.90X245-604[»]
5IUCX-ray1.25A/B399-521[»]
SMRiQ939N5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ939N5. 2 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR019948. Gram-positive_anchor.
IPR022263. KxYKxGKxW.
IPR026465. Ser_adhes_glycop.
[Graphical view]
PfamiPF00746. Gram_pos_anchor. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03715. KxYKxGKxW. 1 hit.
TIGR04224. ser_adhes_Nterm. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGSPB_STRGN
AccessioniPrimary (citable) accession number: Q939N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.