Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial precursor

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Reviewed, UniProtKB/Swiss-Prot Q93873 (NDUS2_CAEEL)

Last modified February 9, 2010. Version 85. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    NADH-ubiquinone oxidoreductase 49 kDa subunit
    Complex I-49kD
      Short name=CI-49kD

Gene names

Name:	gas-1
ORF Names:	K09A9.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster By similarity.
Subunit structure	Complex I is composed of 45 different subunits. Component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side By similarity.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

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Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase receptor-mediated endocytosis Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion Potential

Chain

? – 482

Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

PRO_0000019983

Sites

Metal binding

345

Iron-sulfur (4Fe-4S) Potential

Metal binding

351

Iron-sulfur (4Fe-4S) Potential

Metal binding

366

Iron-sulfur (4Fe-4S) Potential

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Sequences

Sequence

Length

Mass (Da)

Tools

Q93873-1 [UniParc].

Last modified January 1, 1998. Version 2.
Checksum: ACF06535338418B1
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FASTA

482

54,563

        10         20         30         40         50         60 
MLGRKIAGTC LRANVPSVAA TSSTPATQTR NSHTIWYPDA KFERQFKTGG TLGKLWMSER 

        70         80         90        100        110        120 
VSDFDEKIGL DKLEKLAYSD PVMSDNYSGK QREKNLENMI LNFGPQHPAA HGVLRLVLKL 

       130        140        150        160        170        180 
EGEVIIKAIP HIGLLHRATE KLIEHKTYTQ ALPYFDRLDY VSMMCNEQAW SLAVEKLLGI 

       190        200        210        220        230        240 
DIPTRAKYIR TLMGELTRIQ NHIMGITTHA LDVGAMTPFF WMFEEREKLF EFSERVSGAR 

       250        260        270        280        290        300 
MHANYVRPGG VAWDLPIGLM DDIYDWAIKF PERIDELEDM LTENRIWKAR TIDIGLVSAA 

       310        320        330        340        350        360 
DALNWGFSGV MVRGSGIKQD VRKTEPYDAY ADMEFDVPIG TKGDCYDRYL CRIEEMRQSL 

       370        380        390        400        410        420 
NIVHQCLNKM PAGEIKVDDH KVVPPKRAEM KENMESLIHH FKFFTEGFQV PPGATYVPIE 

       430        440        450        460        470        480 
APKGEFGVYL VADGTGKPYR CFIRAPGFAH LAAIHDVCYM SLIADIVAVI GTMDIVFGEV 


DR

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References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	Z79601 Genomic DNA. Translation: CAB01886.1.
PIR	T23532.
RefSeq	NP_510569.1.
UniGene	Cel.8011
3D structure databases
SMR	Q93873. Positions 108-482.
ModBase	Search...
Protein-protein interaction databases
STRING	Q93873.
Genome annotation databases
Ensembl	K09A9.5; K09A9.5; K09A9.5; Caenorhabditis elegans. [Genome view]
GeneID	181646.
KEGG	cel:K09A9.5.
UCSC	K09A9.5. c. elegans.
Organism-specific databases
CTD	181646.
WormBase	WBGene00001520. gas-1.
WormPep	K09A9.5. CE11980. [WorfDB]
Phylogenomic databases
eggNOG	meNOG06746.
HOGENOM	HBG459705.
InParanoid	Q93873.
OMA	YVHRAVE.
PhylomeDB	Q93873.
Enzyme and pathway databases
BRENDA	1.6.5.3. 672. 1.6.99.3. 672.
Gene expression databases
ArrayExpress	Q93873.
Family and domain databases
InterPro	IPR010219. NADH_DH_1_dsu. IPR001135. NADH_Q_OxRdtase_suD. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. [Graphical view]
Pfam	PF00346. Complex1_49kDa. 1 hit. [Graphical view]
TIGRFAMs	TIGR01962. NuoD. 1 hit.
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	914808.

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Entry information

Entry name

NDUS2_CAEEL

Accession

Primary (citable) accession number: Q93873

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents