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Protein

Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Gene

gas-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi345 – 3451Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi351 – 3511Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi366 – 3661Iron-sulfur (4Fe-4S)Sequence Analysis

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. NAD binding Source: InterPro
  4. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  5. quinone binding Source: InterPro

GO - Biological processi

  1. mitochondrial electron transport, NADH to ubiquinone Source: WormBase
  2. response to drug Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-49kD
Short name:
CI-49kD
NADH-ubiquinone oxidoreductase 49 kDa subunit
Gene namesi
Name:gas-1
ORF Names:K09A9.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome X

Organism-specific databases

WormBaseiK09A9.5; CE11980; WBGene00001520; gas-1.

Subcellular locationi

Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

GO - Cellular componenti

  1. intracellular membrane-bounded organelle Source: WormBase
  2. mitochondrial inner membrane Source: UniProtKB-SubCell
  3. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 482Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrialPRO_0000019983
Transit peptidei1 – ?MitochondrionSequence Analysis

Proteomic databases

PaxDbiQ93873.
PRIDEiQ93873.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. Component of the iron-sulfur (IP) fragment of the enzyme (By similarity).By similarity

Protein-protein interaction databases

STRINGi6239.K09A9.5.

Structurei

3D structure databases

ProteinModelPortaliQ93873.
SMRiQ93873. Positions 99-482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0649.
GeneTreeiENSGT00390000009529.
HOGENOMiHOG000228264.
InParanoidiQ93873.
KOiK03935.
OMAiWIQNIPL.
OrthoDBiEOG7WT411.
PhylomeDBiQ93873.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
HAMAPiMF_01358. NDH1_NuoD.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93873-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLGRKIAGTC LRANVPSVAA TSSTPATQTR NSHTIWYPDA KFERQFKTGG
60 70 80 90 100
TLGKLWMSER VSDFDEKIGL DKLEKLAYSD PVMSDNYSGK QREKNLENMI
110 120 130 140 150
LNFGPQHPAA HGVLRLVLKL EGEVIIKAIP HIGLLHRATE KLIEHKTYTQ
160 170 180 190 200
ALPYFDRLDY VSMMCNEQAW SLAVEKLLGI DIPTRAKYIR TLMGELTRIQ
210 220 230 240 250
NHIMGITTHA LDVGAMTPFF WMFEEREKLF EFSERVSGAR MHANYVRPGG
260 270 280 290 300
VAWDLPIGLM DDIYDWAIKF PERIDELEDM LTENRIWKAR TIDIGLVSAA
310 320 330 340 350
DALNWGFSGV MVRGSGIKQD VRKTEPYDAY ADMEFDVPIG TKGDCYDRYL
360 370 380 390 400
CRIEEMRQSL NIVHQCLNKM PAGEIKVDDH KVVPPKRAEM KENMESLIHH
410 420 430 440 450
FKFFTEGFQV PPGATYVPIE APKGEFGVYL VADGTGKPYR CFIRAPGFAH
460 470 480
LAAIHDVCYM SLIADIVAVI GTMDIVFGEV DR
Length:482
Mass (Da):54,563
Last modified:January 1, 1998 - v2
Checksum:iACF06535338418B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z79601 Genomic DNA. Translation: CAB01886.1.
PIRiT23532.
RefSeqiNP_510569.1. NM_078168.4.
UniGeneiCel.8011.

Genome annotation databases

EnsemblMetazoaiK09A9.5; K09A9.5; WBGene00001520.
GeneIDi181646.
KEGGicel:CELE_K09A9.5.
UCSCiK09A9.5. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z79601 Genomic DNA. Translation: CAB01886.1.
PIRiT23532.
RefSeqiNP_510569.1. NM_078168.4.
UniGeneiCel.8011.

3D structure databases

ProteinModelPortaliQ93873.
SMRiQ93873. Positions 99-482.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.K09A9.5.

Proteomic databases

PaxDbiQ93873.
PRIDEiQ93873.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiK09A9.5; K09A9.5; WBGene00001520.
GeneIDi181646.
KEGGicel:CELE_K09A9.5.
UCSCiK09A9.5. c. elegans.

Organism-specific databases

CTDi181646.
WormBaseiK09A9.5; CE11980; WBGene00001520; gas-1.

Phylogenomic databases

eggNOGiCOG0649.
GeneTreeiENSGT00390000009529.
HOGENOMiHOG000228264.
InParanoidiQ93873.
KOiK03935.
OMAiWIQNIPL.
OrthoDBiEOG7WT411.
PhylomeDBiQ93873.

Miscellaneous databases

NextBioi914808.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
HAMAPiMF_01358. NDH1_NuoD.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiNDUS2_CAEEL
AccessioniPrimary (citable) accession number: Q93873
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.