Reviewed, UniProtKB/Swiss-Prot Q93873 (NDUS2_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    NADH-ubiquinone oxidoreductase 49 kDa subunit
    Complex I-49kD
      Short name=CI-49kD

Gene names

Name:	gas-1
ORF Names:	K09A9.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD(+) + ubiquinol. NADH + acceptor = NAD(+) + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster By similarity.
Subunit structure	Complex I is composed of 45 different subunits. Component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix sideBy similarity.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase mitochondrial electron transport, NADH to ubiquinone Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase response to drug Inferred from mutant phenotype. Source: WormBase transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 482		Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial		PRO_0000019983
Sites
	Metal binding	345	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	351	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	366	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q93873-1 [UniParc]. Last modified January 1, 1998. Version 2. Checksum: ACF06535338418B1	FASTA	482	54,563
	10 20 30 40 50 60 MLGRKIAGTC LRANVPSVAA TSSTPATQTR NSHTIWYPDA KFERQFKTGG TLGKLWMSER 70 80 90 100 110 120 VSDFDEKIGL DKLEKLAYSD PVMSDNYSGK QREKNLENMI LNFGPQHPAA HGVLRLVLKL 130 140 150 160 170 180 EGEVIIKAIP HIGLLHRATE KLIEHKTYTQ ALPYFDRLDY VSMMCNEQAW SLAVEKLLGI 190 200 210 220 230 240 DIPTRAKYIR TLMGELTRIQ NHIMGITTHA LDVGAMTPFF WMFEEREKLF EFSERVSGAR 250 260 270 280 290 300 MHANYVRPGG VAWDLPIGLM DDIYDWAIKF PERIDELEDM LTENRIWKAR TIDIGLVSAA 310 320 330 340 350 360 DALNWGFSGV MVRGSGIKQD VRKTEPYDAY ADMEFDVPIG TKGDCYDRYL CRIEEMRQSL 370 380 390 400 410 420 NIVHQCLNKM PAGEIKVDDH KVVPPKRAEM KENMESLIHH FKFFTEGFQV PPGATYVPIE 430 440 450 460 470 480 APKGEFGVYL VADGTGKPYR CFIRAPGFAH LAAIHDVCYM SLIADIVAVI GTMDIVFGEV DR

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z79601 Genomic DNA. Translation: CAB01886.1.
PIR	T23532.
RefSeq	NP_510569.1.
UniGene	Cel.8011
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	K09A9.5. Caenorhabditis elegans. [Contig view]
GeneID	181646.
KEGG	cel:K09A9.5.
Organism-specific databases
WormBase	WBGene00001520. gas-1.
WormPep	K09A9.5. CE11980. [WorfDB]
Gene expression databases
ArrayExpress	Q93873.
Family and domain databases
InterPro	IPR014029. NADH-UbQ_OxRdtase_49kDa_CS. IPR010219. NADH_DH_1_dsu. IPR001135. NADH_UbQ_OxRdtase_49kDa. [Graphical view]
Pfam	PF00346. Complex1_49kDa. 1 hit. [Graphical view]
TIGRFAMs	TIGR01962. NuoD. 1 hit.
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	914808.

Entry information

Entry name

NDUS2_CAEEL

Accession

Primary (citable) accession number: Q93873

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 25, 2008
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project