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Protein

Malate synthase G

Gene

glcB

Organism
Rhizobium leguminosarum bv. viciae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei274 – 2741Acetyl-CoAUniRule annotation
Binding sitei311 – 3111Acetyl-CoAUniRule annotation
Active sitei338 – 3381Proton acceptorUniRule annotation
Binding sitei338 – 3381GlyoxylateUniRule annotation
Metal bindingi430 – 4301MagnesiumUniRule annotation
Binding sitei430 – 4301GlyoxylateUniRule annotation
Metal bindingi458 – 4581MagnesiumUniRule annotation
Binding sitei539 – 5391Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei629 – 6291Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Synonyms:masG
OrganismiRhizobium leguminosarum bv. viciae
Taxonomic identifieri387 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 723723Malate synthase GPRO_0000166897Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei615 – 6151Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi216596.RL0054.

Structurei

3D structure databases

ProteinModelPortaliQ937W7.
SMRiQ937W7. Positions 3-722.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1242Acetyl-CoA bindingUniRule annotation
Regioni455 – 4584Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q937W7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRVDKNGLA IETVLHDFLV EEVLPGLAVD ADKFFADFSA IVHDLAPKNC
60 70 80 90 100
ALLAKRDELQ VKIDDWYRRH GAPADMDEYQ SFLREIGYLL PEGSDFQVST
110 120 130 140 150
QNVDPEIASI AGPQLVVPVM NARYALNAAN ARWGSLYDAL YGTDAIPESD
160 170 180 190 200
GAEKGKSYNP KRGEKVIAWV RDFLDTSAPL QDCRWKDVGS FAVKDGALVV
210 220 230 240 250
RSIDGEQAML TDGKHFAGYR GDAAAPTHIL LKNNGIHIEI VIDAATTIGK
260 270 280 290 300
ADSAHISDVW LESAITTIMD CEDSIAAVDA EDKVVVYRNW LGLMKGDLQE
310 320 330 340 350
EVAKGGTSFI RTLNPDLQYA GPDGAAFEVH RRSLMLVRNV GHLMTNPAIL
360 370 380 390 400
DRDGNEVPEG IMDAAITGLI ALYDIGPSGR RKNSRTGSMY VVKPKMHGPE
410 420 430 440 450
EVAFAVEIFS RVEDALGLPR NTIKMGIMDE ERRTTVNLKE CIRAARERVV
460 470 480 490 500
FINTGFLDRT GDEIHTSMEA GPMIRKGDMR QAAWISAYEN WNVDIGLECG
510 520 530 540 550
LAGHAQIGKG MWAMPDLMAA MLEQKIAHPK AGANTAWVPS PTAATLHATH
560 570 580 590 600
YHRVNVARVQ QGLKDRARAK LSDILSVPVA VRPNWTPEEI QRELDNNAQG
610 620 630 640 650
ILGYVVRWVD QGVGCSKVPD INNVGLMEDR ATLRISAQHM ANWLHHKVVT
660 670 680 690 700
EAQIIETMRR MAAVVDRQNA SDPAYRPMAG NFDDSIAFQA ALDLVLKGRE
710 720
QPNGYTEPVL HRRRLELKAK QAA
Length:723
Mass (Da):79,678
Last modified:December 1, 2001 - v1
Checksum:i4E879906CFD64444
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY059637 Genomic DNA. Translation: AAL17965.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY059637 Genomic DNA. Translation: AAL17965.1.

3D structure databases

ProteinModelPortaliQ937W7.
SMRiQ937W7. Positions 3-722.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216596.RL0054.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Malate synthase gene from Rhizobium leguminosarum."
    Garcia de los Santos A., Hynes M.F.
    Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: VF39.

Entry informationi

Entry nameiMASZ_RHILV
AccessioniPrimary (citable) accession number: Q937W7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: December 1, 2001
Last modified: June 24, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.