Q937L5 (CPNB_COMTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclopentanone 1,2-monooxygenase
Short name=CPMO
EC=1.14.13.16

Gene names

Name:	cpnB
Synonyms:	cpmA

Organism

Comamonas testosteroni (Pseudomonas testosteroni)

Taxonomic identifier

285 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Comamonas

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Cyclopentanone + NADPH + O2 = 5-valerolactone + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Alcohol metabolism; cyclopentanol degradation; 5-valerolactone from cyclopentanol: step 2/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the FAD-binding monooxygenase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro cyclopentanone monooxygenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro flavin-containing monooxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 550	549	Cyclopentanone 1,2-monooxygenase		PRO_0000186454
Regions
	Nucleotide binding	31 – 32	2	FAD By similarity
Sites
	Binding site	51	1	FAD By similarity
	Binding site	60	1	FAD By similarity
	Binding site	71	1	FAD By similarity
	Binding site	77	1	FAD By similarity
	Binding site	123	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Site	344	1	Transition state stabilizer Potential

Sequences

	Sequence		Length	Mass (Da)
	Q937L5 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8956EF95203F6173	FASTA	550	62,111
	10 20 30 40 50 60 MTTMTTMTTE QLGMNNSVND KLDVLLIGAG FTGLYQLYHL RKLGYKVHLV DAGADIGGIW 70 80 90 100 110 120 HWNCYPGARV DTHCQIYQYS IPELWQEFNW KELFPNWAQM REYFHFADKK LDLSKDISFN 130 140 150 160 170 180 TRVQSAVFDE GTREWTVRSI GHQPIQARFV IANLGFGASP STPNVDGIET FKGQWYHTAL 190 200 210 220 230 240 WPQEGVNMAG KRVAIIGTGS SGVQVAQEAA LDAKQVTVYQ RTPNLALPMH QKQLSAEDNL 250 260 270 280 290 300 RMKPELPAAF ERRGKCFAGF DFDFIAKNAT ELSAAERTEI LEELWNAGGF RYWLANFQDY 310 320 330 340 350 360 LFDDKANDYV YEFWRDKVRA RIKDPKVAEK LAPMKKPHPY GAKRPSLEQW YYEIFNQNNV 370 380 390 400 410 420 TLVDVNETPV LRITEKGIVT AEGEAEFDLI VFATGFDAVT GGLTSIDFRN NQGQSFKDVW 430 440 450 460 470 480 SDGIRTQLGV ATAGFPNLLF GYGPQSPAGF CNGPSSAEYQ GDLLIQLMNY LRDNNISRIE 490 500 510 520 530 540 AQSEAQEEWS KLIADFWDSS LFPRAKSWYQ GSNIPGKKVE SLNFPLGLPT YISKFNESAE 550 KGYAGFSLAS

References

[1]

"Cloning of Baeyer-Villiger monooxygenases from Comamonas, Xanthobacter and Rhodococcus via PCR with highly degenerate primers." van Beilen J.B., Mourlane F., Seeger M.A., Kovac J., Li Z., Smits T.H.M., Fritsche U., Witholt B. Environ. Microbiol. 5:174-182(2003) [PubMed: 12588297] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ418060 Genomic DNA. Translation: CAD10798.1.
3D structure databases
ProteinModelPortal	Q937L5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR020946. Flavin_mOase-like. [Graphical view]
Pfam	PF00743. FMO-like. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CPNB_COMTE

Accession

Primary (citable) accession number: Q937L5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	January 23, 2007
Last modified:	June 28, 2011
This is version 43 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program