Reviewed, UniProtKB/Swiss-Prot Q937L4 (CPNA_COMTE)

Names and origin

Protein names

Recommended name:
    Cyclopentanol dehydrogenase
    EC=1.1.1.163

Gene names

Name:	cpnA
Synonyms:	cpmB

Organism

Comamonas testosteroni (Pseudomonas testosteroni)

Taxonomic identifier

285 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Comamonas

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces cyclopentanol to cyclopentanone. The enzyme also shows considerable activity towards cyclohexanol. Ref.1
Catalytic activity	Cyclopentanol + NAD+ = cyclopentanone + NADH.
Pathway	Alcohol metabolism; cyclopentanol degradation; 5-valerolactone from cyclopentanol: step 1/2.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro cyclopentanol dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Cyclopentanol dehydrogenase		PRO_0000054552
Regions
	Nucleotide binding	10 – 34	25	NAD By similarity
Sites
	Active site	155	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q937L4-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 7C42C3CBAEBB858E	FASTA	250	26,626
	10 20 30 40 50 60 MGRVNDKVVL VTGGAMGMGL THCTLLAREG ATVYLSDMNE ELGHQAVAEI RRQGGKAHFL 70 80 90 100 110 120 HLDVTNENHW TGAVDTILAE SDRLDALVNN AGILTLKPVQ DTSNEEWDRI FEINVRSVFL 130 140 150 160 170 180 GTRAVIEPMR KAHKGCIVNV SSIYGLVGAP GAAAYEASKG AVRLFTKACA VDLAPFNIRV 190 200 210 220 230 240 NSVHPGVIAT PMTQQILDAP QSARALLGPT LLGRAAQPME VSQAVLFLVS DEASFVHGSE 250 LVVDGGYTAN

References

[1]

"Cloning of Baeyer-Villiger monooxygenases from Comamonas, Xanthobacter and Rhodococcus via PCR with highly degenerate primers." van Beilen J.B., Mourlane F., Seeger M.A., Kovac J., Li Z., Smits T.H.M., Fritsche U., Witholt B. Environ. Microbiol. 5:174-182(2003) [PubMed: 12588297] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ418060 Genomic DNA. Translation: CAD10799.1.
3D structure databases
HSSP	HSSP built from PDB template 1NFF based on UniProtKB Q10855.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.163. 4566.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
ProtoNet	Search...

Entry information

Entry name

CPNA_COMTE

Accession

Primary (citable) accession number: Q937L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)