Q937F6 (XPKA_LACPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xylulose-5-phosphate phosphoketolase
EC=4.1.2.9

Gene names

Name:

xpkA

Organism

Lactobacillus pentosus

Taxonomic identifier

1589 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	788 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-xylulose 5-phosphate + phosphate = acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O. HAMAP-Rule MF_01403
Cofactor	Thiamine pyrophosphate Potential.
Subunit structure	Homohexamer Potential.
Induction	Induced by sugars that are fermented by the phosphoketolase pathway (PKP) and repressed by glucose mediated by carbon catabolite protein A and by the mannose phosphoenolpyruvate phosphotransferase system. HAMAP-Rule MF_01403
Sequence similarities	Belongs to the XFP family.

Ontologies

Keywords
Ligand	Thiamine pyrophosphate
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	phosphoketolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 788	787	Xylulose-5-phosphate phosphoketolase HAMAP-Rule MF_01403		PRO_0000193868

Sequences

	Sequence		Length	Mass (Da)
	Q937F6 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: C9AE9E7819CF8753	FASTA	788	88,705
	10 20 30 40 50 60 MSTDYSSPAY LQKVDKYWRA ANYLSVGQLY LKDNPLLQRP LKASDVKVHP IGHWGTIAGQ 70 80 90 100 110 120 NFIYAHLNRV INKYGLKMFY VEGPGHGGQV MVSNSYLDGT YTDIYPEITQ DVEGMQKLFK 130 140 150 160 170 180 QFSFPGGVAS HAAPETPGSI HEGGELGYSI SHGVGAILDN PDEIAAVVVG DGESETGPLA 190 200 210 220 230 240 TSWQSTKFIN PINDGAVLPI LNLNGFKISN PTIFGRTSDE KIKQYFESMN WEPIFVEGDD 250 260 270 280 290 300 PEKVHPALAK AMDEAVEKIK AIQKNAREND DATLPVWPMI VFRAPKGWTG PKSWDGDKIE 310 320 330 340 350 360 GSFRAHQIPI PVDQTDMEHA DALVDWLESY QPKELFNEDG SLKDDIKEII PTGDARMAAN 370 380 390 400 410 420 PITNGGVDPK ALNLPNFRDY AVDTSKHGAN VKQDMIVWSD YLRDVIKKNP DNFRLFGPDE 430 440 450 460 470 480 TMSNRLYGVF ETTNRQWMED IHPDSDQYEA PAGRVLDAQL SEHQAEGWLE GYVLTGRHGL 490 500 510 520 530 540 FASYEAFLRV VDSMLTQHFK WLRKANELDW RKKYPSLNII AASTVFQQDH NGYTHQDPGA 550 560 570 580 590 600 LTHLAEKKPE YIREYLPADA NSLLAVGDVI FRSQEKINYV VTSKHPRQQW FSIEEAKQLV 610 620 630 640 650 660 DNGLGIIDWA STDQGSEPDI VFAAAGTEPT LETLAAIQLL HDSFPDMKIR FVNVVDILKL 670 680 690 700 710 720 RSPEKDPRGL SDAEFDHYFT KDKPVVFAFH GYEDLVRDIF FDRHNHNLHV HGYRENGDIT 730 740 750 760 770 780 TPFDVRVMNQ MDRFDLAKSA IAAQPAMENT GAAFVQDMDN MLAKHNAYIR DAGTDLPEVN DWQWKGLK

References

[1]

"Expression of the xylulose 5-phosphate phosphoketolase gene, xpkA, from Lactobacillus pentosus MD363 is induced by sugars that are fermented via the phosphoketolase pathway and is repressed by glucose mediated by CcpA and the mannose phosphoenolpyruvate phosphotransferase system." Posthuma C.C., Bader R., Engelmann R., Postma P.W., Hengstenberg W., Pouwels P.H. Appl. Environ. Microbiol. 68:831-837(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-32; 478-489 AND 651-659, FUNCTION. Strain: MD363.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ309011 Genomic DNA. Translation: CAC84393.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	4.1.2.9. 2886.
Family and domain databases
HAMAP	MF_01403. Phosphoketolase.
InterPro	IPR023962. Phosphoketolase. IPR005593. Xul5P/Fru6P_PKetolase. IPR018969. Xul5P/Fru6P_PKetolase_C. IPR019790. Xul5P/Fru6P_PKetolase_CS. IPR018970. Xul5P/Fru6P_PKetolase_N. IPR019789. Xul5P/Fru6P_PKetolase_ThDP_BS. [Graphical view]
Pfam	PF03894. XFP. 1 hit. PF09363. XFP_C. 1 hit. PF09364. XFP_N. 1 hit. [Graphical view]
PIRSF	PIRSF017245. Phosphoketolase. 1 hit.
PROSITE	PS60002. PHOSPHOKETOLASE_1. 1 hit. PS60003. PHOSPHOKETOLASE_2. 1 hit. PS00187. TPP_ENZYMES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XPKA_LACPE

Accession

Primary (citable) accession number: Q937F6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 38 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program