Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

F-box/WD repeat-containing protein sel-10

Gene

sel-10

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable substrate recognition component of SCF (SKP1-CUL-F-box protein) E3 ubiquitin-protein ligase complex, which mediates the ubiquination and subsequent proteasomal degradation of target proteins (PubMed:17626846). Regulates synapse elimination in early development in the motor neuron HSNL (PubMed:17626846). Cell autonomous negative regulator of lin-12/Notch-mediated signaling, with respect to lin-12 activity in cell fate decisions and tumorigenesis (PubMed:9389650). May target the intracellular domains of lin-12/Notch proteins for ubiquitin-dependent degradation (PubMed:9389650). Involved in sex determination by promoting female development (PubMed:15306688, PubMed:18718460). Potential regulator of presenilin (PubMed:9861048). May have a role in egg laying (PubMed:8293978, PubMed:15306688). Regulates zyg-1 levels (possibly redundantly with lin-23) to control centrosome duplication during mitosis (PubMed:22623721). Negatively regulates lin-45 activity and protein stability, probably by targeting it for ubiquitination and proteasomal degradation (PubMed:23154983).8 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • protein kinase binding Source: UniProtKB

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • female sex differentiation Source: UniProtKB
  • negative regulation of centrosome duplication Source: UniProtKB
  • negative regulation of Notch signaling pathway Source: UniProtKB
  • regulation of Notch signaling pathway Source: WormBase
  • regulation of protein stability Source: UniProtKB
  • regulation of vulval development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Sexual differentiation, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-CEL-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ93794.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box/WD repeat-containing protein sel-10
Alternative name(s):
Egg laying defective protein 411 Publication
Suppressor/enhancer of lin-12 protein 10
Gene namesi
Name:sel-10Imported
Synonyms:egl-41Imported
ORF Names:F55B12.3Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiF55B12.3a; CE25007; WBGene00004767; sel-10.
F55B12.3b; CE27762; WBGene00004767; sel-10.

Subcellular locationi

  • Cell projectionaxon 1 Publication

  • Note: Expressed throughout the axon throughout development.1 Publication

GO - Cellular componenti

  • axon Source: UniProtKB
  • cytoplasm Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cell projection

Pathology & Biotechi

Disruption phenotypei

Worms show a weak masculinization phenotype (PubMed:8293978). Moreover sel-10 mutants suppress the egg laying defective (egl) phenotype of the sel-12 mutants (PubMed:8293978). RNAi-mediated knockdown results in increased zyg-1 expression at centrosomes (PubMed:22623721).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi567 – 5671G → E in n1074; masculinizing phenotype. Does not self-interact in vitro or bind to skr-1 protein. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 587587F-box/WD repeat-containing protein sel-10PRO_0000051212Add
BLAST

Proteomic databases

PaxDbiQ93794.
PRIDEiQ93794.

PTM databases

iPTMnetiQ93794.

Expressioni

Developmental stagei

Expressed in the HSNL motor neuron from early larval stage L4 to the young adult stage.1 Publication

Interactioni

Subunit structurei

Probable component of the SCF(sel-10) E3 ubiquitin-protein ligase complex which includes skr-1 and F-box domain-containing protein sel-10 as a substrate recognition component (PubMed:17626846). Interacts with fem-1, fem-2, and fem-3 (PubMed:15306688). Interacts with the intracellular domain of glp-1 and sel-12 (PubMed:9861048). Interacts with lin-12 (PubMed:9389650). Interacts with skr-1 (PubMed:18718460). Interacts with zyg-1 (PubMed:22623721).6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
fem-1P172212EBI-323098,EBI-1998155
fem-2P495942EBI-323098,EBI-1998402
fem-3P346912EBI-323098,EBI-445465
lin-12P145853EBI-323098,EBI-326049
Notch4P316952EBI-323098,EBI-643670From a different organism.
skr-1G5ECU15EBI-323098,EBI-323117

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi44892. 11 interactions.
IntActiQ93794. 11 interactions.
MINTiMINT-115740.
STRINGi6239.F55B12.3a.

Structurei

3D structure databases

ProteinModelPortaliQ93794.
SMRiQ93794. Positions 106-583.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini121 – 16747F-boxPROSITE-ProRule annotationAdd
BLAST
Repeati253 – 29139WD 1Add
BLAST
Repeati294 – 33340WD 2Add
BLAST
Repeati336 – 37338WD 3Add
BLAST
Repeati376 – 41540WD 4Add
BLAST
Repeati416 – 45540WD 5Add
BLAST
Repeati461 – 49838WD 6Add
BLAST
Repeati501 – 53939WD 7Add
BLAST

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0274. Eukaryota.
ENOG410XRWX. LUCA.
GeneTreeiENSGT00760000119106.
HOGENOMiHOG000236264.
InParanoidiQ93794.
KOiK10260.
OMAiGGCVHTL.
OrthoDBiEOG7VX8VF.
PhylomeDBiQ93794.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR001810. F-box_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00256. FBOX. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
PS00678. WD_REPEATS_1. 5 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q93794-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWPRNDVHMD DGSMTPEDQE PVTDNDMEYN DNGEESSYSN GSSSSYNADK
60 70 80 90 100
LSSSRPLQHK LDLSASPSRN NDLNPRVEHL IALFKDLSSA EQMDAFTRLL
110 120 130 140 150
QESNMTNIRQ LRAIIEPHFQ RDFLSCLPVE LGMKILHNLT GYDLLKVAQV
160 170 180 190 200
SKNWKLISEI DKIWKSLGVE EFKHHPDPTD RVTGAWQGTA IAAGVTIPDH
210 220 230 240 250
IQPCDLNVHR FLKLQKFGDI FERAADKSRY LRADKIEKNW NANPIMGSAV
260 270 280 290 300
LRGHEDHVIT CMQIHDDVLV TGSDDNTLKV WCIDKGEVMY TLVGHTGGVW
310 320 330 340 350
TSQISQCGRY IVSGSTDRTV KVWSTVDGSL LHTLQGHTST VRCMAMAGSI
360 370 380 390 400
LVTGSRDTTL RVWDVESGRH LATLHGHHAA VRCVQFDGTT VVSGGYDFTV
410 420 430 440 450
KIWNAHTGRC IRTLTGHNNR VYSLLFESER SIVCSGSLDT SIRVWDFTRP
460 470 480 490 500
EGQECVALLQ GHTSLTSGMQ LRGNILVSCN ADSHVRVWDI HEGTCVHMLS
510 520 530 540 550
GHRSAITSLQ WFGRNMVATS SDDGTVKLWD IERGALIRDL VTLDSGGNGG
560 570 580
CIWRLCSTST MLACAVGSRN NTEETKVILL DFDAVYP
Length:587
Mass (Da):65,312
Last modified:April 13, 2004 - v3
Checksum:i2D3970B4EFAA1B8C
GO
Isoform b (identifier: Q93794-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-55: Missing.

Note: No experimental confirmation available.
Show »
Length:585
Mass (Da):65,068
Checksum:i60F100B591F28D1B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei54 – 552Missing in isoform b. CuratedVSP_009813

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020788 mRNA. Translation: AAC47809.1.
Z79757 Genomic DNA. Translation: CAB02129.2.
Z79757 Genomic DNA. Translation: CAC42307.1.
PIRiT22703.
RefSeqiNP_001023975.1. NM_001028804.2. [Q93794-2]
NP_506421.1. NM_074020.5. [Q93794-1]
UniGeneiCel.2743.

Genome annotation databases

EnsemblMetazoaiF55B12.3a; F55B12.3a; WBGene00004767. [Q93794-1]
GeneIDi179878.
KEGGicel:CELE_F55B12.3.
UCSCiF55B12.3b.1. c. elegans. [Q93794-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020788 mRNA. Translation: AAC47809.1.
Z79757 Genomic DNA. Translation: CAB02129.2.
Z79757 Genomic DNA. Translation: CAC42307.1.
PIRiT22703.
RefSeqiNP_001023975.1. NM_001028804.2. [Q93794-2]
NP_506421.1. NM_074020.5. [Q93794-1]
UniGeneiCel.2743.

3D structure databases

ProteinModelPortaliQ93794.
SMRiQ93794. Positions 106-583.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi44892. 11 interactions.
IntActiQ93794. 11 interactions.
MINTiMINT-115740.
STRINGi6239.F55B12.3a.

PTM databases

iPTMnetiQ93794.

Proteomic databases

PaxDbiQ93794.
PRIDEiQ93794.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF55B12.3a; F55B12.3a; WBGene00004767. [Q93794-1]
GeneIDi179878.
KEGGicel:CELE_F55B12.3.
UCSCiF55B12.3b.1. c. elegans. [Q93794-1]

Organism-specific databases

CTDi179878.
WormBaseiF55B12.3a; CE25007; WBGene00004767; sel-10.
F55B12.3b; CE27762; WBGene00004767; sel-10.

Phylogenomic databases

eggNOGiKOG0274. Eukaryota.
ENOG410XRWX. LUCA.
GeneTreeiENSGT00760000119106.
HOGENOMiHOG000236264.
InParanoidiQ93794.
KOiK10260.
OMAiGGCVHTL.
OrthoDBiEOG7VX8VF.
PhylomeDBiQ93794.

Enzyme and pathway databases

ReactomeiR-CEL-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ93794.

Miscellaneous databases

PROiQ93794.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR001810. F-box_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00256. FBOX. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
PS00678. WD_REPEATS_1. 5 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "sel-10, a negative regulator of lin-12 activity in Caenorhabditis elegans, encodes a member of the CDC4 family of proteins."
    Hubbard E.J.A., Wu G., Kitajewski J., Greenwald I.
    Genes Dev. 11:3182-3193(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, INTERACTION WITH LIN-12.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "Suppressors of a lin-12 hypomorph define genes that interact with both lin-12 and glp-1 in Caenorhabditis elegans."
    Sundaram M., Greenwald I.
    Genetics 135:765-783(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  4. "Evidence for functional and physical association between Caenorhabditis elegans SEL-10, a Cdc4p-related protein, and SEL-12 presenilin."
    Wu G., Hubbard E.J.A., Kitajewski J.K., Greenwald I.
    Proc. Natl. Acad. Sci. U.S.A. 95:15787-15791(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GLP-1 AND SEL-12.
  5. "The Caenorhabditis elegans F-box protein SEL-10 promotes female development and may target FEM-1 and FEM-3 for degradation by the proteasome."
    Jaeger S., Schwartz H.T., Horvitz H.R., Conradt B.
    Proc. Natl. Acad. Sci. U.S.A. 101:12549-12554(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FEM-1; FEM-2 AND FEM-3.
  6. "Spatial regulation of an E3 ubiquitin ligase directs selective synapse elimination."
    Ding M., Chao D., Wang G., Shen K.
    Science 317:947-951(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  7. "SKR-1, a homolog of Skp1 and a member of the SCF(SEL-10) complex, regulates sex-determination and LIN-12/Notch signaling in C. elegans."
    Killian D.J., Harvey E., Johnson P., Otori M., Mitani S., Xue D.
    Dev. Biol. 322:322-331(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SKR-1, MUTAGENESIS OF GLY-567.
  8. "The C. elegans F-box proteins LIN-23 and SEL-10 antagonize centrosome duplication by regulating ZYG-1 levels."
    Peel N., Dougherty M., Goeres J., Liu Y., O'Connell K.F.
    J. Cell Sci. 125:3535-3544(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ZYG-1, DISRUPTION PHENOTYPE.
  9. "SEL-10/Fbw7-dependent negative feedback regulation of LIN-45/Braf signaling in C. elegans via a conserved phosphodegron."
    de la Cova C., Greenwald I.
    Genes Dev. 26:2524-2535(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSEL10_CAEEL
AccessioniPrimary (citable) accession number: Q93794
Secondary accession number(s): O44083, Q95ZT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 13, 2004
Last modified: June 8, 2016
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.