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Protein

NEDD8

Gene

ned-8

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex uba-3-ula-1 and linkage to the E2 enzyme ubc-12. Attachment of ned-8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.3 Publications

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
  • negative regulation of gene expression Source: UniProtKB
  • positive regulation of apoptotic process Source: WormBase

Keywordsi

Molecular functionDevelopmental protein
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-CEL-5689603. UCH proteinases.
R-CEL-8856825. Cargo recognition for clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8
Alternative name(s):
Neddylin
Protein NED-8
Ubiquitin-like protein Nedd8
Gene namesi
Name:ned-8
ORF Names:F45H11.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiF45H11.2; CE10552; WBGene00003587; ned-8.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • nucleus Source: WormBase

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Worms either arrest during embryonic development, or show vulval eversion at the L4 stage and burst at the vulva during the L4-to-adult molt. Those who survive to the adult stage display severe defects in terminal differentiation of seam cells, vulva and male tail.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000427771 – 76NEDD8Add BLAST76
PropeptideiPRO_000004277877By similarity1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Post-translational modificationi

Cleavage of precursor form is necessary for function.By similarity

Keywords - PTMi

Isopeptide bond

Proteomic databases

EPDiQ93725.
PaxDbiQ93725.
PeptideAtlasiQ93725.
PRIDEiQ93725.

Expressioni

Developmental stagei

Expressed throughout development.1 Publication

Gene expression databases

BgeeiWBGene00003587.

Interactioni

Subunit structurei

Interacts with dcn-1. Covalently attached to cullins.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei8Interaction with uba-3By similarity1
Sitei44Interaction with uba-3By similarity1

Protein-protein interaction databases

BioGridi38327. 3 interactors.
IntActiQ93725. 1 interactor.
STRINGi6239.F45H11.2.

Structurei

3D structure databases

ProteinModelPortaliQ93725.
SMRiQ93725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 72Interaction with uba-3By similarity3

Sequence similaritiesi

Belongs to the ubiquitin family.Curated

Phylogenomic databases

eggNOGiKOG0005. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00870000136558.
HOGENOMiHOG000233942.
InParanoidiQ93725.
KOiK12158.
OMAiGKQMTDD.
OrthoDBiEOG091H0BM6.
PhylomeDBiQ93725.

Family and domain databases

InterProiView protein in InterPro
IPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
PfamiView protein in Pfam
PF00240. ubiquitin. 1 hit.
PRINTSiPR00348. UBIQUITIN.
SMARTiView protein in SMART
SM00213. UBQ. 1 hit.
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93725-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIKVKTLTG KEIELDIEPN DRVERIKEKV EEKEGIPPPQ QRLIFAGKQM
60 70
NDDKTAADYK VLGGSVLHLV LALRGGF
Length:77
Mass (Da):8,629
Last modified:February 1, 1997 - v1
Checksum:iF3387DE33C671C78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z78420 Genomic DNA. Translation: CAB01708.1.
PIRiT22249.
RefSeqiNP_492717.1. NM_060316.3.
UniGeneiCel.18712.

Genome annotation databases

EnsemblMetazoaiF45H11.2; F45H11.2; WBGene00003587.
GeneIDi172910.
KEGGicel:CELE_F45H11.2.
UCSCiF45H11.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z78420 Genomic DNA. Translation: CAB01708.1.
PIRiT22249.
RefSeqiNP_492717.1. NM_060316.3.
UniGeneiCel.18712.

3D structure databases

ProteinModelPortaliQ93725.
SMRiQ93725.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi38327. 3 interactors.
IntActiQ93725. 1 interactor.
STRINGi6239.F45H11.2.

Proteomic databases

EPDiQ93725.
PaxDbiQ93725.
PeptideAtlasiQ93725.
PRIDEiQ93725.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF45H11.2; F45H11.2; WBGene00003587.
GeneIDi172910.
KEGGicel:CELE_F45H11.2.
UCSCiF45H11.2. c. elegans.

Organism-specific databases

CTDi172910.
WormBaseiF45H11.2; CE10552; WBGene00003587; ned-8.

Phylogenomic databases

eggNOGiKOG0005. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00870000136558.
HOGENOMiHOG000233942.
InParanoidiQ93725.
KOiK12158.
OMAiGKQMTDD.
OrthoDBiEOG091H0BM6.
PhylomeDBiQ93725.

Enzyme and pathway databases

ReactomeiR-CEL-5689603. UCH proteinases.
R-CEL-8856825. Cargo recognition for clathrin-mediated endocytosis.

Miscellaneous databases

PROiQ93725.

Gene expression databases

BgeeiWBGene00003587.

Family and domain databases

InterProiView protein in InterPro
IPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
PfamiView protein in Pfam
PF00240. ubiquitin. 1 hit.
PRINTSiPR00348. UBIQUITIN.
SMARTiView protein in SMART
SM00213. UBQ. 1 hit.
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNEDD8_CAEEL
AccessioniPrimary (citable) accession number: Q93725
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: February 1, 1997
Last modified: February 15, 2017
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.