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Protein

NEDD8

Gene

ned-8

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex uba-3-ula-1 and linkage to the E2 enzyme ubc-12. Attachment of ned-8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.3 Publications

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
  • negative regulation of gene expression Source: UniProtKB
  • positive regulation of apoptotic process Source: WormBase

Keywordsi

Molecular functionDevelopmental protein
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-CEL-5689603 UCH proteinases
R-CEL-8856825 Cargo recognition for clathrin-mediated endocytosis
R-CEL-8951664 Neddylation

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8
Alternative name(s):
Neddylin
Protein NED-8
Ubiquitin-like protein Nedd8
Gene namesi
Name:ned-8
ORF Names:F45H11.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiF45H11.2 ; CE10552 ; WBGene00003587 ; ned-8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Worms either arrest during embryonic development, or show vulval eversion at the L4 stage and burst at the vulva during the L4-to-adult molt. Those who survive to the adult stage display severe defects in terminal differentiation of seam cells, vulva and male tail.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000427771 – 76NEDD8Add BLAST76
PropeptideiPRO_000004277877By similarity1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Post-translational modificationi

Cleavage of precursor form is necessary for function.By similarity

Keywords - PTMi

Isopeptide bond

Proteomic databases

EPDiQ93725
PaxDbiQ93725
PeptideAtlasiQ93725
PRIDEiQ93725

Expressioni

Developmental stagei

Expressed throughout development.1 Publication

Gene expression databases

BgeeiWBGene00003587

Interactioni

Subunit structurei

Interacts with dcn-1 (PubMed:15988528). Covalently attached to cullins (PubMed:12781129). May interact with atx-3 (PubMed:17935801).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei8Interaction with uba-3By similarity1
Sitei44Interaction with uba-3By similarity1

Protein-protein interaction databases

BioGridi38327, 3 interactors
IntActiQ93725, 1 interactor
STRINGi6239.F45H11.2

Structurei

3D structure databases

ProteinModelPortaliQ93725
SMRiQ93725
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 72Interaction with uba-3By similarity3

Sequence similaritiesi

Belongs to the ubiquitin family.Curated

Phylogenomic databases

eggNOGiKOG0005 Eukaryota
COG5272 LUCA
GeneTreeiENSGT00900000141147
HOGENOMiHOG000233942
InParanoidiQ93725
KOiK12158
OMAiGKQMTDD
OrthoDBiEOG091G178I
PhylomeDBiQ93725

Family and domain databases

CDDicd01806 Nedd8, 1 hit
InterProiView protein in InterPro
IPR038738 Nedd8-like
IPR019956 Ubiquitin
IPR029071 Ubiquitin-like_domsf
IPR019954 Ubiquitin_CS
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF00240 ubiquitin, 1 hit
PRINTSiPR00348 UBIQUITIN
SMARTiView protein in SMART
SM00213 UBQ, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00299 UBIQUITIN_1, 1 hit
PS50053 UBIQUITIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93725-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIKVKTLTG KEIELDIEPN DRVERIKEKV EEKEGIPPPQ QRLIFAGKQM
60 70
NDDKTAADYK VLGGSVLHLV LALRGGF
Length:77
Mass (Da):8,629
Last modified:February 1, 1997 - v1
Checksum:iF3387DE33C671C78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z78420 Genomic DNA Translation: CAB01708.1
PIRiT22249
RefSeqiNP_492717.1, NM_060316.3
UniGeneiCel.18712

Genome annotation databases

EnsemblMetazoaiF45H11.2; F45H11.2; WBGene00003587
GeneIDi172910
KEGGicel:CELE_F45H11.2
UCSCiF45H11.2 c. elegans

Similar proteinsi

Entry informationi

Entry nameiNEDD8_CAEEL
AccessioniPrimary (citable) accession number: Q93725
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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