Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NEDD8

Gene

ned-8

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex uba-3-ula-1 and linkage to the E2 enzyme ubc-12. Attachment of ned-8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.3 Publications

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
  • negative regulation of gene expression Source: UniProtKB
  • positive regulation of apoptotic process Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8
Alternative name(s):
Neddylin
Protein NED-8
Ubiquitin-like protein Nedd8
Gene namesi
Name:ned-8
ORF Names:F45H11.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiF45H11.2; CE10552; WBGene00003587; ned-8.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • nucleus Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Worms either arrest during embryonic development, or show vulval eversion at the L4 stage and burst at the vulva during the L4-to-adult molt. Those who survive to the adult stage display severe defects in terminal differentiation of seam cells, vulva and male tail.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7676NEDD8PRO_0000042777Add
BLAST
Propeptidei77 – 771By similarityPRO_0000042778

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki76 – 76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Post-translational modificationi

Cleavage of precursor form is necessary for function.By similarity

Keywords - PTMi

Isopeptide bond

Proteomic databases

EPDiQ93725.
PaxDbiQ93725.
PRIDEiQ93725.

Expressioni

Developmental stagei

Expressed throughout development.1 Publication

Interactioni

Subunit structurei

Interacts with dcn-1. Covalently attached to cullins.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei8 – 81Interaction with uba-3By similarity
Sitei44 – 441Interaction with uba-3By similarity

Protein-protein interaction databases

BioGridi38327. 3 interactions.
IntActiQ93725. 1 interaction.
STRINGi6239.F45H11.2.

Structurei

3D structure databases

ProteinModelPortaliQ93725.
SMRiQ93725. Positions 1-76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni70 – 723Interaction with uba-3By similarity

Sequence similaritiesi

Belongs to the ubiquitin family.Curated

Phylogenomic databases

eggNOGiKOG0005. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00840000129851.
HOGENOMiHOG000233942.
InParanoidiQ93725.
KOiK12158.
OMAiGKQMTDD.
OrthoDBiEOG7JDR1W.
PhylomeDBiQ93725.

Family and domain databases

InterProiIPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93725-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIKVKTLTG KEIELDIEPN DRVERIKEKV EEKEGIPPPQ QRLIFAGKQM
60 70
NDDKTAADYK VLGGSVLHLV LALRGGF
Length:77
Mass (Da):8,629
Last modified:February 1, 1997 - v1
Checksum:iF3387DE33C671C78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z78420 Genomic DNA. Translation: CAB01708.1.
PIRiT22249.
RefSeqiNP_492717.1. NM_060316.3.
UniGeneiCel.18712.

Genome annotation databases

EnsemblMetazoaiF45H11.2; F45H11.2; WBGene00003587.
GeneIDi172910.
KEGGicel:CELE_F45H11.2.
UCSCiF45H11.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z78420 Genomic DNA. Translation: CAB01708.1.
PIRiT22249.
RefSeqiNP_492717.1. NM_060316.3.
UniGeneiCel.18712.

3D structure databases

ProteinModelPortaliQ93725.
SMRiQ93725. Positions 1-76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi38327. 3 interactions.
IntActiQ93725. 1 interaction.
STRINGi6239.F45H11.2.

Proteomic databases

EPDiQ93725.
PaxDbiQ93725.
PRIDEiQ93725.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF45H11.2; F45H11.2; WBGene00003587.
GeneIDi172910.
KEGGicel:CELE_F45H11.2.
UCSCiF45H11.2. c. elegans.

Organism-specific databases

CTDi172910.
WormBaseiF45H11.2; CE10552; WBGene00003587; ned-8.

Phylogenomic databases

eggNOGiKOG0005. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00840000129851.
HOGENOMiHOG000233942.
InParanoidiQ93725.
KOiK12158.
OMAiGKQMTDD.
OrthoDBiEOG7JDR1W.
PhylomeDBiQ93725.

Miscellaneous databases

NextBioi877495.
PROiQ93725.

Family and domain databases

InterProiIPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "The NED-8 conjugating system in Caenorhabditis elegans is required for embryogenesis and terminal differentiation of the hypodermis."
    Jones D., Candido E.P.M.
    Dev. Biol. 226:152-165(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, FUNCTION, DISRUPTION PHENOTYPE.
  3. "Cytoskeletal regulation by the Nedd8 ubiquitin-like protein modification pathway."
    Kurz T., Pintard L., Willis J.H., Hamill D.R., Goenczy P., Peter M., Bowerman B.
    Science 295:1294-1298(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION.
  4. "Neddylation and deneddylation of CUL-3 is required to target MEI-1/katanin for degradation at the meiosis-to-mitosis transition in C. elegans."
    Pintard L., Kurz T., Glaser S., Willis J.H., Peter M., Bowerman B.
    Curr. Biol. 13:911-921(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  5. "The conserved protein DCN-1/Dcn1p is required for cullin neddylation in C. elegans and S. cerevisiae."
    Kurz T., Oezlue N., Rudolf F., O'Rourke S.M., Luke B., Hofmann K., Hyman A.A., Bowerman B., Peter M.
    Nature 435:1257-1261(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DCN-1.

Entry informationi

Entry nameiNEDD8_CAEEL
AccessioniPrimary (citable) accession number: Q93725
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: February 1, 1997
Last modified: May 11, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.