Reviewed, UniProtKB/Swiss-Prot Q93714 (IDH3A_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
    EC=1.1.1.41
Alternative name(s):
    Isocitric dehydrogenase subunit alpha
    NAD(+)-specific ICDH subunit alpha

Gene names

ORF Names:

F43G9.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA interference Inferred from mutant phenotype. Source: WormBase body morphogenesis Inferred from mutant phenotype. Source: WormBase determination of adult lifespan Inferred from mutant phenotype. Source: WormBase embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase receptor-mediated endocytosis Inferred from mutant phenotype. Source: WormBase tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
C37E2.1	Q93353	1	EBI-316891,EBI-316885

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 358		Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial		PRO_0000014441
Sites
	Metal binding	226	1	Magnesium or manganese By similarity
	Metal binding	250	1	Magnesium or manganese By similarity
	Metal binding	254	1	Magnesium or manganese By similarity
	Binding site	108	1	Substrate By similarity
	Binding site	118	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	226	1	Substrate By similarity
	Site	146	1	Critical for catalysis By similarity
	Site	193	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q93714-1 [UniParc]. Last modified April 13, 2004. Version 3. Checksum: B175DC68C127610F	FASTA	358	38,466
	10 20 30 40 50 60 MLGKCIKKAS STVGQSIRYS SGDVRRVTLI PGDGIGPEIS ASVQKIFEAA DAPIAWDPVD 70 80 90 100 110 120 VTPVKGRDGV FRIPSRCIEL MHANKVGLKG PLETPIGKGH RSLNLAVRKE FSLYANVRPC 130 140 150 160 170 180 RSLEGHKTLY DNVDVVTIRE NTEGEYSGIE HEIVPGVVQS IKLITETASR NVASFAFEYA 190 200 210 220 230 240 RQNGRKVVTA VHKANIMRQS DGLFLSICRE QAALYPDIKF KEAYLDTVCL NMVQDPSQYD 250 260 270 280 290 300 VLVMPNLYGD ILSDLCAGLV GGLGVTPSGN IGKGAAVFES VHGTAPDIAG QDKANPTALL 310 320 330 340 350 LSAVMMLRYM NLPQHAARIE KAVFDAIADG RAKTGDLGGT GTCSSFTADV CARVKDLE

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z79755 Genomic DNA. Translation: CAB02111.2.
PIR	T22149.
RefSeq	NP_492330.2.
UniGene	Cel.17675
3D structure databases
SMR	Q93714. Positions 18-354.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q93714. 6 interactions.
STRING	Q93714.
Genome annotation databases
Ensembl	F43G9.1.1; F43G9.1.1; F43G9.1; Caenorhabditis elegans. [Genome view] F43G9.1.2; F43G9.1.2; F43G9.1; Caenorhabditis elegans. [Genome view]
GeneID	172655.
KEGG	cel:F43G9.1.
UCSC	F43G9.1.1. c. elegans.
Organism-specific databases
CTD	172655.
WormBase	WBGene00009664. F43G9.1.
WormPep	F43G9.1. CE34018. [WorfDB]
Phylogenomic databases
eggNOG	meNOG04733.
HOGENOM	HBG518924.
InParanoid	Q93714.
OMA	ITTFAYE.
PhylomeDB	Q93714.
Enzyme and pathway databases
BRENDA	1.1.1.41. 672.
Gene expression databases
ArrayExpress	Q93714.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD_mit. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	876445.

Entry information

Entry name

IDH3A_CAEEL

Accession

Primary (citable) accession number: Q93714

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 13, 2004
Last modified:	February 9, 2010
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project