Q93714 (IDH3A_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
EC=1.1.1.41

Alternative name(s):
Isocitric dehydrogenase subunit alpha
NAD(+)-specific ICDH subunit alpha

Gene names

ORF Names:

F43G9.1

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrion Inferred from direct assay PubMed 20188671. Source: WormBase
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 358		Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial		PRO_0000014441
Sites
	Metal binding	226	1	Magnesium or manganese By similarity
	Metal binding	250	1	Magnesium or manganese By similarity
	Metal binding	254	1	Magnesium or manganese By similarity
	Binding site	108	1	Substrate By similarity
	Binding site	118	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	226	1	Substrate By similarity
	Site	146	1	Critical for catalysis By similarity
	Site	193	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q93714 [UniParc]. Last modified April 13, 2004. Version 3. Checksum: B175DC68C127610F	FASTA	358	38,466
	10 20 30 40 50 60 MLGKCIKKAS STVGQSIRYS SGDVRRVTLI PGDGIGPEIS ASVQKIFEAA DAPIAWDPVD 70 80 90 100 110 120 VTPVKGRDGV FRIPSRCIEL MHANKVGLKG PLETPIGKGH RSLNLAVRKE FSLYANVRPC 130 140 150 160 170 180 RSLEGHKTLY DNVDVVTIRE NTEGEYSGIE HEIVPGVVQS IKLITETASR NVASFAFEYA 190 200 210 220 230 240 RQNGRKVVTA VHKANIMRQS DGLFLSICRE QAALYPDIKF KEAYLDTVCL NMVQDPSQYD 250 260 270 280 290 300 VLVMPNLYGD ILSDLCAGLV GGLGVTPSGN IGKGAAVFES VHGTAPDIAG QDKANPTALL 310 320 330 340 350 LSAVMMLRYM NLPQHAARIE KAVFDAIADG RAKTGDLGGT GTCSSFTADV CARVKDLE

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z79755 Genomic DNA. Translation: CAB02111.2.
PIR	T22149.
RefSeq	NP_492330.2. NM_059929.3.
3D structure databases
ProteinModelPortal	Q93714.
SMR	Q93714. Positions 27-354.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q93714. 2 interactions.
MINT	MINT-1058740.
STRING	6239.F43G9.1.2.
2D gel databases
World-2DPAGE	0020:Q93714.
Proteomic databases
PaxDb	Q93714.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F43G9.1.1; F43G9.1.1; F43G9.1. F43G9.1.2; F43G9.1.2; F43G9.1.
GeneID	172655.
KEGG	cel:CELE_F43G9.1.
UCSC	F43G9.1.1. c. elegans.
Organism-specific databases
CTD	172655.
WormBase	F43G9.1; CE34018; WBGene00009664.
Phylogenomic databases
eggNOG	COG0473.
GeneTree	ENSGT00550000074918.
HOGENOM	HOG000021113.
InParanoid	Q93714.
KO	K00030.
OMA	NQKQVTR.
Family and domain databases
Gene3D	3.40.718.10. 1 hit.
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
PANTHER	PTHR11835. PTHR11835. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	876445.

Entry information

Entry name

IDH3A_CAEEL

Accession

Primary (citable) accession number: Q93714

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 13, 2004
Last modified:	May 1, 2013
This is version 102 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project