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Q93714 (IDH3A_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial

EC=1.1.1.41
Alternative name(s):
Isocitric dehydrogenase subunit alpha
NAD(+)-specific ICDH subunit alpha
Gene names
Name:idha-1
ORF Names:F43G9.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Subunit structure

Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandMagnesium
Manganese
Metal-binding
NAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmitochondrion

Inferred from direct assay PubMed 20188671. Source: WormBase

   Molecular_functionNAD binding

Inferred from electronic annotation. Source: InterPro

isocitrate dehydrogenase (NAD+) activity

Inferred from electronic annotation. Source: UniProtKB-EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 358Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrialPRO_0000014441

Sites

Metal binding2261Magnesium or manganese By similarity
Metal binding2501Magnesium or manganese By similarity
Metal binding2541Magnesium or manganese By similarity
Binding site1081Substrate By similarity
Binding site1181Substrate By similarity
Binding site1391Substrate By similarity
Binding site2261Substrate By similarity
Site1461Critical for catalysis By similarity
Site1931Critical for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q93714 [UniParc].

Last modified April 13, 2004. Version 3.
Checksum: B175DC68C127610F

FASTA35838,466
        10         20         30         40         50         60 
MLGKCIKKAS STVGQSIRYS SGDVRRVTLI PGDGIGPEIS ASVQKIFEAA DAPIAWDPVD 

        70         80         90        100        110        120 
VTPVKGRDGV FRIPSRCIEL MHANKVGLKG PLETPIGKGH RSLNLAVRKE FSLYANVRPC 

       130        140        150        160        170        180 
RSLEGHKTLY DNVDVVTIRE NTEGEYSGIE HEIVPGVVQS IKLITETASR NVASFAFEYA 

       190        200        210        220        230        240 
RQNGRKVVTA VHKANIMRQS DGLFLSICRE QAALYPDIKF KEAYLDTVCL NMVQDPSQYD 

       250        260        270        280        290        300 
VLVMPNLYGD ILSDLCAGLV GGLGVTPSGN IGKGAAVFES VHGTAPDIAG QDKANPTALL 

       310        320        330        340        350 
LSAVMMLRYM NLPQHAARIE KAVFDAIADG RAKTGDLGGT GTCSSFTADV CARVKDLE 

« Hide

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z79755 Genomic DNA. Translation: CAB02111.2.
PIRT22149.
RefSeqNP_492330.2. NM_059929.3.
UniGeneCel.17675.

3D structure databases

ProteinModelPortalQ93714.
SMRQ93714. Positions 27-354.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid38088. 2 interactions.
IntActQ93714. 2 interactions.
MINTMINT-1058740.
STRING6239.F43G9.1.2.

2D gel databases

World-2DPAGE0020:Q93714.

Proteomic databases

PaxDbQ93714.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF43G9.1.1; F43G9.1.1; F43G9.1.
F43G9.1.2; F43G9.1.2; F43G9.1.
GeneID172655.
KEGGcel:CELE_F43G9.1.
UCSCF43G9.1.1. c. elegans.

Organism-specific databases

CTD172655.
WormBaseF43G9.1; CE34018; WBGene00009664; idha-1.

Phylogenomic databases

eggNOGCOG0473.
HOGENOMHOG000021113.
InParanoidQ93714.
KOK00030.
OMAEGMYSGL.
PhylomeDBQ93714.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11835. PTHR11835. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00175. mito_nad_idh. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio876445.
PROQ93714.

Entry information

Entry nameIDH3A_CAEEL
AccessionPrimary (citable) accession number: Q93714
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 13, 2004
Last modified: April 16, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase