Reviewed, UniProtKB/Swiss-Prot Q93353 (IDH3B_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
    EC=1.1.1.41
Alternative name(s):
    Isocitric dehydrogenase
    NAD(+)-specific ICDH

Gene names

ORF Names:

C37E2.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
F43G9.1	Q93714	1	EBI-316885,EBI-316891

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 31	31	Mitochondrion By similarity
	Chain	32 – 379	348	Probable isocitrate dehydrogenase [NAD] subunit beta, mitochondrial		PRO_0000014448
Regions
	Nucleotide binding	44 – 72	29	NAD Potential
Sites
	Metal binding	248	1	Magnesium or manganese By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	161	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity
	Site	168	1	Critical for catalysis By similarity
	Site	215	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q93353-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: F099425960AB3086	FASTA	379	41,553
	10 20 30 40 50 60 MLSRTVSSLS RVAPQTLGAV NAASSRQYSI TAPRPPTELN QKLKVTIIPG DGVGPELIYT 70 80 90 100 110 120 VQDIVKQTGI PIEFEEIFLS EVHYTRSSSI ENAVESIGRN NNVALKGAIE ESAVLHTEGE 130 140 150 160 170 180 LQGLNMRLRR SLDLFANVVH IKTLDGIKTR HGKQLDFVIV REQTEGEYSS LEHELVPGVI 190 200 210 220 230 240 ECLKISTRTK AERIAKFAFD YATKTGRKKV TAVHKANIMK LGDGLFLRTC EGVAKQYPKI 250 260 270 280 290 300 QFESMIIDNT CMQLVSKPEQ FDVMVMPNLY GNIIDNLAAG LVGGAGVVPG QSVGRDFVIF 310 320 330 340 350 360 EPGSRHSFQE AMGRSIANPT AMILCAANML NHLHLDAWGN SLRQAVADVV KEGKVRTRDL 370 GGYATTVDFA DAVIDKFRI

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z81046 Genomic DNA. Translation: CAB02822.1.
PIR	T19810.
RefSeq	NP_510362.1.
UniGene	Cel.8914
3D structure databases
HSSP	HSSP built from PDB template 1DPZ based on UniProtKB P00351.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:27476N.
IntAct	Q93353. 1 interaction.
Genome annotation databases
Ensembl	C37E2.1. Caenorhabditis elegans. [Contig view]
GeneID	181528.
KEGG	cel:C37E2.1.
Organism-specific databases
WormBase	WBGene00007993. C37E2.1.
WormPep	C37E2.1. CE08620. [WorfDB]
Phylogenomic databases
OMA	Q93353. FEKMIVD.
Enzyme and pathway databases
BRENDA	1.1.1.41. 672.
Gene expression databases
ArrayExpress	Q93353.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD_mit. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	914316.

Entry information

Entry name

IDH3B_CAEEL

Accession

Primary (citable) accession number: Q93353

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project