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Q93341

- DYR_CAEEL

UniProt

Q93341 - DYR_CAEEL

Protein

Putative dihydrofolate reductase

Gene

dhfr-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei9 – 91NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei69 – 691SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 217NADPBy similarity
    Nucleotide bindingi53 – 553NADPBy similarity
    Nucleotide bindingi75 – 773NADPBy similarity
    Nucleotide bindingi115 – 1228NADPBy similarity

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB-EC
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. glycine biosynthetic process Source: InterPro
    2. nucleotide biosynthetic process Source: InterPro
    3. one-carbon metabolic process Source: UniProtKB-KW
    4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    ReactomeiREACT_183103. G1/S-Specific Transcription.
    REACT_183242. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative dihydrofolate reductase (EC:1.5.1.3)
    Gene namesi
    Name:dhfr-1
    ORF Names:C36B1.7
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome I

    Organism-specific databases

    WormBaseiC36B1.7; CE05374; WBGene00007974; dhfr-1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 189189Putative dihydrofolate reductasePRO_0000186372Add
    BLAST

    Proteomic databases

    PaxDbiQ93341.
    PRIDEiQ93341.

    Interactioni

    Protein-protein interaction databases

    BioGridi38114. 1 interaction.
    IntActiQ93341. 1 interaction.
    STRINGi6239.C36B1.7.

    Structurei

    3D structure databases

    ProteinModelPortaliQ93341.
    SMRiQ93341. Positions 1-185.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 185183DHFRPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni29 – 346Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the dihydrofolate reductase family.Curated
    Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0262.
    GeneTreeiENSGT00390000010283.
    HOGENOMiHOG000040235.
    InParanoidiQ93341.
    KOiK00287.
    OMAiIKKDMQY.
    OrthoDBiEOG7V1FS3.
    PhylomeDBiQ93341.

    Family and domain databases

    Gene3Di3.40.430.10. 1 hit.
    InterProiIPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    [Graphical view]
    PRINTSiPR00070. DHFR.
    SUPFAMiSSF53597. SSF53597. 1 hit.
    PROSITEiPS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q93341-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKMNLIVAM DAEGGIGKNG VLPWRIKKDM QYFASVTKNV SDQSKRNAVL    50
    MGRKCWESIP VTRRPLAGRL NIVLSRQLPA QKSDDYIVVN SLEAAMKLLS 100
    EPPFVDSIET IWNIGGAEIY DLALRENLVD EIHLTRIFKN FEADVHLKSL 150
    DFSKMEKVQN AEVSSENSEI FEENGLKFEF CKWKVVENH 189
    Length:189
    Mass (Da):21,602
    Last modified:February 1, 1997 - v1
    Checksum:iC1642558E097725F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z80215 Genomic DNA. Translation: CAB02272.1.
    PIRiT19778.
    RefSeqiNP_492364.1. NM_059963.3.
    UniGeneiCel.19146.

    Genome annotation databases

    EnsemblMetazoaiC36B1.7; C36B1.7; WBGene00007974.
    GeneIDi172681.
    KEGGicel:CELE_C36B1.7.
    UCSCiC36B1.7. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z80215 Genomic DNA. Translation: CAB02272.1 .
    PIRi T19778.
    RefSeqi NP_492364.1. NM_059963.3.
    UniGenei Cel.19146.

    3D structure databases

    ProteinModelPortali Q93341.
    SMRi Q93341. Positions 1-185.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 38114. 1 interaction.
    IntActi Q93341. 1 interaction.
    STRINGi 6239.C36B1.7.

    Proteomic databases

    PaxDbi Q93341.
    PRIDEi Q93341.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C36B1.7 ; C36B1.7 ; WBGene00007974 .
    GeneIDi 172681.
    KEGGi cel:CELE_C36B1.7.
    UCSCi C36B1.7. c. elegans.

    Organism-specific databases

    CTDi 172681.
    WormBasei C36B1.7 ; CE05374 ; WBGene00007974 ; dhfr-1.

    Phylogenomic databases

    eggNOGi COG0262.
    GeneTreei ENSGT00390000010283.
    HOGENOMi HOG000040235.
    InParanoidi Q93341.
    KOi K00287.
    OMAi IKKDMQY.
    OrthoDBi EOG7V1FS3.
    PhylomeDBi Q93341.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .
    Reactomei REACT_183103. G1/S-Specific Transcription.
    REACT_183242. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

    Miscellaneous databases

    NextBioi 876555.
    PROi Q93341.

    Family and domain databases

    Gene3Di 3.40.430.10. 1 hit.
    InterProi IPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00070. DHFR.
    SUPFAMi SSF53597. SSF53597. 1 hit.
    PROSITEi PS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiDYR_CAEEL
    AccessioniPrimary (citable) accession number: Q93341
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3