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Q93341 (DYR_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:dhfr-1
ORF Names:C36B1.7
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length189 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 189189Putative dihydrofolate reductase
PRO_0000186372

Regions

Domain3 – 185183DHFR
Nucleotide binding15 – 217NADP By similarity
Nucleotide binding53 – 553NADP By similarity
Nucleotide binding75 – 773NADP By similarity
Nucleotide binding115 – 1228NADP By similarity
Region29 – 346Substrate binding By similarity

Sites

Binding site91NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding site691Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q93341 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: C1642558E097725F

FASTA18921,602
        10         20         30         40         50         60 
MRKMNLIVAM DAEGGIGKNG VLPWRIKKDM QYFASVTKNV SDQSKRNAVL MGRKCWESIP 

        70         80         90        100        110        120 
VTRRPLAGRL NIVLSRQLPA QKSDDYIVVN SLEAAMKLLS EPPFVDSIET IWNIGGAEIY 

       130        140        150        160        170        180 
DLALRENLVD EIHLTRIFKN FEADVHLKSL DFSKMEKVQN AEVSSENSEI FEENGLKFEF 


CKWKVVENH 

« Hide

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z80215 Genomic DNA. Translation: CAB02272.1.
PIRT19778.
RefSeqNP_492364.1. NM_059963.3.
UniGeneCel.19146.

3D structure databases

ProteinModelPortalQ93341.
SMRQ93341. Positions 1-185.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid38114. 1 interaction.
IntActQ93341. 1 interaction.
STRING6239.C36B1.7.

Proteomic databases

PaxDbQ93341.
PRIDEQ93341.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC36B1.7; C36B1.7; C36B1.7.
GeneID172681.
KEGGcel:CELE_C36B1.7.
UCSCC36B1.7. c. elegans.

Organism-specific databases

CTD172681.
WormBaseC36B1.7; CE05374; WBGene00007974; dhfr-1.

Phylogenomic databases

eggNOGCOG0262.
HOGENOMHOG000040235.
InParanoidQ93341.
KOK00287.
OMASIETIWN.
OrthoDBEOG7V1FS3.
PhylomeDBQ93341.

Enzyme and pathway databases

UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio876555.
PROQ93341.

Entry information

Entry nameDYR_CAEEL
AccessionPrimary (citable) accession number: Q93341
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase