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Protein

Putative dihydrofolate reductase

Gene

dhfr-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathway: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative dihydrofolate reductase (dhfr-1)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91NADP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei69 – 691SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 217NADPBy similarity
Nucleotide bindingi53 – 553NADPBy similarity
Nucleotide bindingi75 – 773NADPBy similarity
Nucleotide bindingi115 – 1228NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_306849. Metabolism of folate and pterines.
REACT_309220. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
REACT_328624. G1/S-Specific Transcription.
REACT_349225. E2F mediated regulation of DNA replication.
UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:dhfr-1
ORF Names:C36B1.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiC36B1.7; CE05374; WBGene00007974; dhfr-1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 189189Putative dihydrofolate reductasePRO_0000186372Add
BLAST

Proteomic databases

PaxDbiQ93341.
PRIDEiQ93341.

Interactioni

Protein-protein interaction databases

BioGridi38114. 1 interaction.
IntActiQ93341. 1 interaction.
STRINGi6239.C36B1.7.

Structurei

3D structure databases

ProteinModelPortaliQ93341.
SMRiQ93341. Positions 1-180.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 185183DHFRPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni29 – 346Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0262.
GeneTreeiENSGT00390000010283.
HOGENOMiHOG000040235.
InParanoidiQ93341.
KOiK00287.
OMAiCWESIPE.
OrthoDBiEOG7V1FS3.
PhylomeDBiQ93341.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q93341-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKMNLIVAM DAEGGIGKNG VLPWRIKKDM QYFASVTKNV SDQSKRNAVL
60 70 80 90 100
MGRKCWESIP VTRRPLAGRL NIVLSRQLPA QKSDDYIVVN SLEAAMKLLS
110 120 130 140 150
EPPFVDSIET IWNIGGAEIY DLALRENLVD EIHLTRIFKN FEADVHLKSL
160 170 180
DFSKMEKVQN AEVSSENSEI FEENGLKFEF CKWKVVENH
Length:189
Mass (Da):21,602
Last modified:February 1, 1997 - v1
Checksum:iC1642558E097725F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z80215 Genomic DNA. Translation: CAB02272.1.
PIRiT19778.
RefSeqiNP_492364.1. NM_059963.3.
UniGeneiCel.19146.

Genome annotation databases

EnsemblMetazoaiC36B1.7; C36B1.7; WBGene00007974.
GeneIDi172681.
KEGGicel:CELE_C36B1.7.
UCSCiC36B1.7. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z80215 Genomic DNA. Translation: CAB02272.1.
PIRiT19778.
RefSeqiNP_492364.1. NM_059963.3.
UniGeneiCel.19146.

3D structure databases

ProteinModelPortaliQ93341.
SMRiQ93341. Positions 1-180.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi38114. 1 interaction.
IntActiQ93341. 1 interaction.
STRINGi6239.C36B1.7.

Proteomic databases

PaxDbiQ93341.
PRIDEiQ93341.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC36B1.7; C36B1.7; WBGene00007974.
GeneIDi172681.
KEGGicel:CELE_C36B1.7.
UCSCiC36B1.7. c. elegans.

Organism-specific databases

CTDi172681.
WormBaseiC36B1.7; CE05374; WBGene00007974; dhfr-1.

Phylogenomic databases

eggNOGiCOG0262.
GeneTreeiENSGT00390000010283.
HOGENOMiHOG000040235.
InParanoidiQ93341.
KOiK00287.
OMAiCWESIPE.
OrthoDBiEOG7V1FS3.
PhylomeDBiQ93341.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.
ReactomeiREACT_306849. Metabolism of folate and pterines.
REACT_309220. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
REACT_328624. G1/S-Specific Transcription.
REACT_349225. E2F mediated regulation of DNA replication.

Miscellaneous databases

NextBioi876555.
PROiQ93341.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiDYR_CAEEL
AccessioniPrimary (citable) accession number: Q93341
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 24, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.