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Protein

Putative dihydrofolate reductase

Gene

dhfr-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative dihydrofolate reductase (dhfr-1)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei69SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 21NADPBy similarity7
Nucleotide bindingi53 – 55NADPBy similarity3
Nucleotide bindingi75 – 77NADPBy similarity3
Nucleotide bindingi115 – 122NADPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

ReactomeiR-CEL-196757 Metabolism of folate and pterines
UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Putative dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:dhfr-1
ORF Names:C36B1.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiC36B1.7 ; CE05374 ; WBGene00007974 ; dhfr-1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863721 – 189Putative dihydrofolate reductaseAdd BLAST189

Proteomic databases

EPDiQ93341
PaxDbiQ93341
PeptideAtlasiQ93341
PRIDEiQ93341

Expressioni

Gene expression databases

BgeeiWBGene00007974

Interactioni

Protein-protein interaction databases

BioGridi38114, 1 interactor
IntActiQ93341, 1 interactor
STRINGi6239.C36B1.7

Structurei

3D structure databases

ProteinModelPortaliQ93341
SMRiQ93341
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 185DHFRPROSITE-ProRule annotationAdd BLAST183

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 34Substrate bindingBy similarity6

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

eggNOGiKOG1324 Eukaryota
COG0262 LUCA
GeneTreeiENSGT00390000010283
HOGENOMiHOG000040235
InParanoidiQ93341
KOiK00287
OMAiNTWESIP
OrthoDBiEOG091G0PRK
PhylomeDBiQ93341

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q93341-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKMNLIVAM DAEGGIGKNG VLPWRIKKDM QYFASVTKNV SDQSKRNAVL
60 70 80 90 100
MGRKCWESIP VTRRPLAGRL NIVLSRQLPA QKSDDYIVVN SLEAAMKLLS
110 120 130 140 150
EPPFVDSIET IWNIGGAEIY DLALRENLVD EIHLTRIFKN FEADVHLKSL
160 170 180
DFSKMEKVQN AEVSSENSEI FEENGLKFEF CKWKVVENH
Length:189
Mass (Da):21,602
Last modified:February 1, 1997 - v1
Checksum:iC1642558E097725F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z80215 Genomic DNA Translation: CAB02272.1
PIRiT19778
RefSeqiNP_492364.1, NM_059963.3
UniGeneiCel.19146

Genome annotation databases

EnsemblMetazoaiC36B1.7; C36B1.7; WBGene00007974
GeneIDi172681
KEGGicel:CELE_C36B1.7
UCSCiC36B1.7 c. elegans

Similar proteinsi

Entry informationi

Entry nameiDYR_CAEEL
AccessioniPrimary (citable) accession number: Q93341
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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