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Q93324 (MANBA_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable beta-mannosidase
EC=3.2.1.25
Alternative name(s):
Mannanase
Short name=Mannase
Gene names
ORF Names:C33G3.4
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length900 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.

Subcellular location

Lysosome Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
   Cellular componentLysosome
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentlysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-mannosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 900879Probable beta-mannosidase
PRO_0000012167

Sites

Active site4631Proton donor By similarity

Amino acid modifications

Glycosylation381N-linked (GlcNAc...) Potential
Glycosylation421N-linked (GlcNAc...) Potential
Glycosylation1311N-linked (GlcNAc...) Ref.2
Glycosylation4771N-linked (GlcNAc...) Ref.2
Glycosylation5761N-linked (GlcNAc...) Potential
Glycosylation6611N-linked (GlcNAc...) Potential
Glycosylation7381N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q93324 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: C6B90C25517579C7

FASTA900103,978
        10         20         30         40         50         60 
MRTSLVVCLF WLLFQLHTTH GYNTLVNLAG NWEFSSSNKT VNGTGTVPGD IYSDLYASGI 

        70         80         90        100        110        120 
IDNPLFGENH LNLKWIAEDD WTYSRKFRLI DLDDTVGAFL EIESVDTIAT VYVNGQKVLH 

       130        140        150        160        170        180 
SRNQFLPYHV NVTDIIALGE NDITIKFKSS VKYAEKRADE YKKIFGHSLP PDCNPDIYHG 

       190        200        210        220        230        240 
ECHQNFIRKA QYSFAWDWGP SFPTVGIPST ITINIYRGQY FHDFNWKTRF AHGKWKVAFE 

       250        260        270        280        290        300 
FDTFHYGART IEYSVQIPEL GIKESDYYRL SATKSLQTRS KNIMSLSIPM EHEPERWWPN 

       310        320        330        340        350        360 
GMGEQKLYDV VVSMGGQVKE KKIGFKTVEL VQDLIDPKKP EKGRNFYFKI NDEPVFLKGT 

       370        380        390        400        410        420 
NWIPVSMFRS DRENIAKTEF LLDSVAEVGM NAIRVWGGGF YESNHFYYYA SKKGILVWQD 

       430        440        450        460        470        480 
LMFACALYPT TEEFIQNAEE EVSYNVDRIS QHTSVIVFSG NNENEAAIRG HWWKASNYTE 

       490        500        510        520        530        540 
SQQVKDYVLL YQRLAKIAKK VAPTIPFIMS SPSNGVETEE EGGVSKNPYD VRYGDIHYYN 

       550        560        570        580        590        600 
EFVNLWRDDT YLTPRCASEY GVQSYPMKET MLNWINESDW EYTSKAMFHR QHHPGGIATN 

       610        620        630        640        650        660 
LLMIFQHLPI PAECGSKSVS DVPSCKYISS ASYMSRLAYF SQVHQSIALK TQTLHYRRFR 

       670        680        690        700        710        720 
NTTTNEGLGN TMCAMYWQLN DVWAAPTWST IDFEQNWKMA HYEARRFFSN VAVYSFADET 

       730        740        750        760        770        780 
DFNLKVFLLN DNPYLLHNIT VNVQMLSWGN GLDPILTNEF HIDSVPAGSS EVLKTGITFS 

       790        800        810        820        830        840 
KITELSEYLY VSTLYDSSGV KIHEDVLVPD FLFEVDFNTF GDVQISDVQR IDEKTYDLTI 

       850        860        870        880        890        900 
TTDRVSPFTW ITCKKPFTGW FSDNGFHMIQ RLRKIRLIAK FEVDLEKSDF TVCNLKNCYV

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References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[2]"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-131 AND ASN-477, MASS SPECTROMETRY.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z78540 Genomic DNA. Translation: CAB01737.1.
PIRT19689.
RefSeqNP_510342.1. NM_077941.5.

3D structure databases

ProteinModelPortalQ93324.
SMRQ93324. Positions 28-771.
ModBaseSearch...

Protein-protein interaction databases

STRING6239.C33G3.4.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Proteomic databases

PaxDbQ93324.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC33G3.4; C33G3.4; C33G3.4.
GeneID181517.
KEGGcel:CELE_C33G3.4.
UCSCC33G3.4. c. elegans.

Organism-specific databases

CTD181517.
WormBaseC33G3.4; CE08560; WBGene00007904.

Phylogenomic databases

eggNOGCOG3250.
GeneTreeENSGT00390000001670.
HOGENOMHOG000186862.
InParanoidQ93324.
KOK01192.
OMAYGARTIE.

Family and domain databases

Gene3D2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00703. Glyco_hydro_2. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMSSF49785. Gal_bind_like. 1 hit.
SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNetSearch...

Other

NextBio914270.

Entry information

Entry nameMANBA_CAEEL
AccessionPrimary (citable) accession number: Q93324
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

SIMILARITY comments

Index of protein domains and families

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