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Protein

Probable beta-mannosidase

Gene

C33G3.4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei463 – 4631Proton donorBy similarity

GO - Molecular functioni

  1. beta-mannosidase activity Source: GO_Central

GO - Biological processi

  1. carbohydrate metabolic process Source: GO_Central
  2. mannan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-mannosidase (EC:3.2.1.25)
Alternative name(s):
Mannanase
Short name:
Mannase
Gene namesi
ORF Names:C33G3.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome X

Organism-specific databases

WormBaseiC33G3.4; CE08560; WBGene00007904.

Subcellular locationi

Lysosome Curated

GO - Cellular componenti

  1. intracellular membrane-bounded organelle Source: GO_Central
  2. lysosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 900879Probable beta-mannosidasePRO_0000012167Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi42 – 421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi131 – 1311N-linked (GlcNAc...)1 Publication
Glycosylationi477 – 4771N-linked (GlcNAc...)1 Publication
Glycosylationi576 – 5761N-linked (GlcNAc...)Sequence Analysis
Glycosylationi661 – 6611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi738 – 7381N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ93324.
PRIDEiQ93324.

Interactioni

Protein-protein interaction databases

STRINGi6239.C33G3.4.

Structurei

3D structure databases

ProteinModelPortaliQ93324.
SMRiQ93324. Positions 28-771.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3250.
GeneTreeiENSGT00390000001670.
HOGENOMiHOG000186862.
InParanoidiQ93324.
KOiK01192.
OMAiIATNLLM.
OrthoDBiEOG7S7SD2.
PhylomeDBiQ93324.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view]
PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93324-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRTSLVVCLF WLLFQLHTTH GYNTLVNLAG NWEFSSSNKT VNGTGTVPGD
60 70 80 90 100
IYSDLYASGI IDNPLFGENH LNLKWIAEDD WTYSRKFRLI DLDDTVGAFL
110 120 130 140 150
EIESVDTIAT VYVNGQKVLH SRNQFLPYHV NVTDIIALGE NDITIKFKSS
160 170 180 190 200
VKYAEKRADE YKKIFGHSLP PDCNPDIYHG ECHQNFIRKA QYSFAWDWGP
210 220 230 240 250
SFPTVGIPST ITINIYRGQY FHDFNWKTRF AHGKWKVAFE FDTFHYGART
260 270 280 290 300
IEYSVQIPEL GIKESDYYRL SATKSLQTRS KNIMSLSIPM EHEPERWWPN
310 320 330 340 350
GMGEQKLYDV VVSMGGQVKE KKIGFKTVEL VQDLIDPKKP EKGRNFYFKI
360 370 380 390 400
NDEPVFLKGT NWIPVSMFRS DRENIAKTEF LLDSVAEVGM NAIRVWGGGF
410 420 430 440 450
YESNHFYYYA SKKGILVWQD LMFACALYPT TEEFIQNAEE EVSYNVDRIS
460 470 480 490 500
QHTSVIVFSG NNENEAAIRG HWWKASNYTE SQQVKDYVLL YQRLAKIAKK
510 520 530 540 550
VAPTIPFIMS SPSNGVETEE EGGVSKNPYD VRYGDIHYYN EFVNLWRDDT
560 570 580 590 600
YLTPRCASEY GVQSYPMKET MLNWINESDW EYTSKAMFHR QHHPGGIATN
610 620 630 640 650
LLMIFQHLPI PAECGSKSVS DVPSCKYISS ASYMSRLAYF SQVHQSIALK
660 670 680 690 700
TQTLHYRRFR NTTTNEGLGN TMCAMYWQLN DVWAAPTWST IDFEQNWKMA
710 720 730 740 750
HYEARRFFSN VAVYSFADET DFNLKVFLLN DNPYLLHNIT VNVQMLSWGN
760 770 780 790 800
GLDPILTNEF HIDSVPAGSS EVLKTGITFS KITELSEYLY VSTLYDSSGV
810 820 830 840 850
KIHEDVLVPD FLFEVDFNTF GDVQISDVQR IDEKTYDLTI TTDRVSPFTW
860 870 880 890 900
ITCKKPFTGW FSDNGFHMIQ RLRKIRLIAK FEVDLEKSDF TVCNLKNCYV
Length:900
Mass (Da):103,978
Last modified:February 1, 1997 - v1
Checksum:iC6B90C25517579C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z78540 Genomic DNA. Translation: CAB01737.1.
PIRiT19689.
RefSeqiNP_510342.1. NM_077941.5.
UniGeneiCel.5667.

Genome annotation databases

EnsemblMetazoaiC33G3.4; C33G3.4; WBGene00007904.
GeneIDi181517.
KEGGicel:CELE_C33G3.4.
UCSCiC33G3.4. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z78540 Genomic DNA. Translation: CAB01737.1.
PIRiT19689.
RefSeqiNP_510342.1. NM_077941.5.
UniGeneiCel.5667.

3D structure databases

ProteinModelPortaliQ93324.
SMRiQ93324. Positions 28-771.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C33G3.4.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Proteomic databases

PaxDbiQ93324.
PRIDEiQ93324.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC33G3.4; C33G3.4; WBGene00007904.
GeneIDi181517.
KEGGicel:CELE_C33G3.4.
UCSCiC33G3.4. c. elegans.

Organism-specific databases

CTDi181517.
WormBaseiC33G3.4; CE08560; WBGene00007904.

Phylogenomic databases

eggNOGiCOG3250.
GeneTreeiENSGT00390000001670.
HOGENOMiHOG000186862.
InParanoidiQ93324.
KOiK01192.
OMAiIATNLLM.
OrthoDBiEOG7S7SD2.
PhylomeDBiQ93324.

Miscellaneous databases

NextBioi914270.
PROiQ93324.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view]
PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-131 AND ASN-477, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiMANBA_CAEEL
AccessioniPrimary (citable) accession number: Q93324
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: February 4, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.