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Protein

DNA gyrase subunit A

Gene

gyrA

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.UniRule annotation

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei123O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
Biological processAntibiotic resistance
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR158878:G1G22-6-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesi
Name:gyrAUniRule annotation
Ordered Locus Names:SAV0006
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2097174

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001452521 – 889DNA gyrase subunit AAdd BLAST889

Proteomic databases

PaxDbiQ932M0
PRIDEiQ932M0

2D gel databases

World-2DPAGE0002:Q932M0

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.UniRule annotation

Protein-protein interaction databases

STRINGi158878.SAV0006

Structurei

3D structure databases

ProteinModelPortaliQ932M0
SMRiQ932M0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi528 – 534GyrA-boxUniRule annotation7

Sequence similaritiesi

Belongs to the type II topoisomerase GyrA/ParC subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C24 Bacteria
COG0188 LUCA
HOGENOMiHOG000076278
KOiK02469
OMAiTHHWLLF
PhylomeDBiQ932M0

Family and domain databases

Gene3Di1.10.268.10, 1 hit
2.120.10.90, 1 hit
3.90.199.10, 3 hits
HAMAPiMF_01897 GyrA, 1 hit
InterProiView protein in InterPro
IPR005743 GyrA
IPR006691 GyrA/parC_rep
IPR035516 Gyrase/topoIV_suA_C
IPR013760 Topo_IIA-like_dom_sf
IPR002205 Topo_IIA_A/C
IPR013758 Topo_IIA_A/C_ab
IPR013757 Topo_IIA_A_a_sf
PfamiView protein in Pfam
PF03989 DNA_gyraseA_C, 6 hits
PF00521 DNA_topoisoIV, 1 hit
SMARTiView protein in SMART
SM00434 TOP4c, 1 hit
SUPFAMiSSF101904 SSF101904, 1 hit
SSF56719 SSF56719, 1 hit

Sequencei

Sequence statusi: Complete.

Q932M0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELPQSRIN ERNITSEMRE SFLDYAMSVI VARALPDVRD GLKPVHRRIL
60 70 80 90 100
YGLNEQGMTP DKSYKKSARI VGDVMGKYHP HGDLSIYEAM VRMAQDFSYR
110 120 130 140 150
YPLVDGQGNF GSMDGDGAAA MRYTEARMTK ITLELLRDIN KDTIDFIDNY
160 170 180 190 200
DGNEREPSVL PARFPNLLAN GASGIAVGMA TNIPPHNLTE LINGVLSLSK
210 220 230 240 250
NPDISIAELM EDIEGPDFPT AGLILGKSGI RRAYETGRGS IQMRSRAVIE
260 270 280 290 300
ERGGGRQRIV VTEIPFQVNK ARMIEKIAEL VRDKKIDGIT DLRDETSLRT
310 320 330 340 350
GVRVVIDVRK DANASVILNN LYKQTPLQTS FGVNMIALVN GRPKLINLKE
360 370 380 390 400
ALVHYLEHQK TVVRRRTQYN LRKAKDRAHI LEGLRIALDH IDEIISTIRE
410 420 430 440 450
SDTDKVAMKS LQQRFKLSEK QAQAILDMRL RRLTGLERDK IEAEYNELLN
460 470 480 490 500
YISELETILA DEEVLLQLVR DELTEIRDRF GDDRRTEIQL GGFEDLEDED
510 520 530 540 550
LIPEEQIVIT LSHNNYIKRL PVSTYRAQNR GGRGVQGMNT LEEDFVSQLV
560 570 580 590 600
TLSTHDHVLF FTNKGRVYKL KGYEVPELSR QSKGIPVVNA IELENDEVIS
610 620 630 640 650
TMIAVKDLES EDNFLVFATK RGVVKRSALS NFSRINRNGK IAISFREDDE
660 670 680 690 700
LIAVRLTSGQ EDILIGTSHA SLIRFPESTL RPLGRTATGV KGITLREGDE
710 720 730 740 750
VVGLDVAHAN SVDEVLVVTE NGYGKRTPVN DYRLSNRGGK GIKTATITER
760 770 780 790 800
NGNVVCITTV TGEEDLMIVT NAGVIIRLDV ADISQNGRAA QGVRLIRLGD
810 820 830 840 850
DQFVSTVAKV KEDAEDETNE DEQSTSTVSE DGTEQQREAV VNDETPGNAI
860 870 880
HTEVIDSEEN DEDGRIEVRQ DFMDRVEEDI QQSSDEDEE
Length:889
Mass (Da):99,650
Last modified:December 1, 2001 - v1
Checksum:i4A533AA0A1AC4943
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA Translation: BAB56168.1
RefSeqiWP_000819068.1, NC_002758.2

Genome annotation databases

EnsemblBacteriaiBAB56168; BAB56168; SAV0006
KEGGisav:SAV0006

Similar proteinsi

Entry informationi

Entry nameiGYRA_STAAM
AccessioniPrimary (citable) accession number: Q932M0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health