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Protein

Zinc metalloproteinase nas-37

Gene

nas-37

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metalloprotease that plays an essential role in molting, a process during larval stages in which a new cuticle is formed and the old cuticle is shed. Required during ecdysis, the opening of the cuticle to allow the worm to escape.2 Publications

Catalytic activityi

Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi204Zinc; catalyticBy similarity1
Active sitei205By similarity1
Metal bindingi208Zinc; catalyticBy similarity1
Metal bindingi214Zinc; catalyticBy similarity1

GO - Molecular functioni

  • metalloendopeptidase activity Source: WormBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • collagen and cuticulin-based cuticle development Source: WormBase
  • ecdysis, collagen and cuticulin-based cuticle Source: WormBase
  • molting cycle, collagen and cuticulin-based cuticle Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.318.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase nas-37 (EC:3.4.24.21)
Alternative name(s):
Nematode astacin 37
Gene namesi
Name:nas-37
ORF Names:C17G1.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC17G1.6a; CE31417; WBGene00003553; nas-37.
C17G1.6b; CE38034; WBGene00003553; nas-37.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Worms exhibit incomplete ecdysis; at each molt the cuticle fails to open sufficiently at the anterior end and the partially shed cuticle is dragged behind the animal.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi214H → R in ox196; induces defects in protease involved in ecdysis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000002894123 – 765Zinc metalloproteinase nas-37Add BLAST743

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi126N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi307 ↔ 317By similarity
Disulfide bondi311 ↔ 331By similarity
Disulfide bondi333 ↔ 342By similarity
Disulfide bondi350 ↔ 374By similarity
Disulfide bondi400 ↔ 420By similarity
Disulfide bondi588 ↔ 621By similarity
Disulfide bondi592 ↔ 626By similarity
Disulfide bondi604 ↔ 611By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ93243.
PaxDbiQ93243.
PeptideAtlasiQ93243.
PRIDEiQ93243.

Expressioni

Tissue specificityi

Expressed in hypodermal cells. Not expressed in the seam cells in L1 to L3 larvae, but it is present in seam cells of L4 larvae. Also expressed in attachment points of the cuticle at the anterior end of larvae, in the arcade cells in the mouth, the anterior pharynx, the amphid socket cells, and in the rectal epithelial cells at the posterior end of the larvae (at protein level).2 Publications

Developmental stagei

Present in hypodermal cells of the anterior cuticle 4 hours before each molt and is shed in the cuticle after ecdysis.

Gene expression databases

BgeeiWBGene00003553.

Interactioni

Protein-protein interaction databases

STRINGi6239.C17G1.6a.

Structurei

3D structure databases

ProteinModelPortaliQ93243.
SMRiQ93243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini303 – 343EGF-likePROSITE-ProRule annotationAdd BLAST41
Domaini350 – 458CUBPROSITE-ProRule annotationAdd BLAST109
Domaini576 – 627TSP type-1PROSITE-ProRule annotationAdd BLAST52

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi527 – 534Poly-Ser8

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 1 CUB domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 TSP type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000016555.
InParanoidiQ93243.
OMAiYCETVEY.
OrthoDBiEOG091G0N5F.
PhylomeDBiQ93243.

Family and domain databases

CDDicd00041. CUB. 1 hit.
Gene3Di2.60.120.290. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000859. CUB_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR017050. Metallopeptidase_nem.
IPR033955. NAS-37.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
IPR000884. TSP1_rpt.
[Graphical view]
PANTHERiPTHR10127:SF602. PTHR10127:SF602. 3 hits.
PfamiPF01400. Astacin. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
PIRSFiPIRSF036365. Astacin_nematoda. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 1 hit.
SM00209. TSP1. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01180. CUB. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50092. TSP1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q93243-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSQACLKVC LALIGLVSIV STAYIANDVV SDYAEVKELL AAFYRKHAKK
60 70 80 90 100
YGHDYDPAAI QAIAENMDKS VKNDKTEATV NRKLWNEVFE NDIILTLPQA
110 120 130 140 150
ESLLSESNSP RSRRQAHPDP RNFWPNLTIS YEFYGGEETW RQLIRSAIRH
160 170 180 190 200
VEQNVCFKFK ENGGDRDGLR YYRGNGCWSN VGRVGGRQLV SIGYGCDSLG
210 220 230 240 250
IVSHETLHAL GLWHEQSRDD RDNFISIVAD KITRGTEGNF AKRTAANSDN
260 270 280 290 300
LGQPYDLGSV MHYGAKSFAY DWSSDTIKTR DWRYQNTIGQ RDGLSFKDAK
310 320 330 340 350
MINTRYCSNV CQRSLPCLNE GYTDPNNCGR CRCPSGYGGT YCETVEYTSC
360 370 380 390 400
GGSLTASSSY KKIESGIVQP DANCVWRIRN PGGNVEVMFD QVNFQCADPC
410 420 430 440 450
QSYVEVKYLS QKTSTGARLC CSLPSVIRSE GDDVIIILRG TPNTAVGWRG
460 470 480 490 500
FTLKYRAIGG TPITPATVRP TYATTTRPYW TRTASGWIHI KNPPLYKPDG
510 520 530 540 550
QIYTSDEQSA ETKYSSEELY DPSTFLSPSS SSASPALLLP SDASPQRPSA
560 570 580 590 600
QEHDLSQLSQ NALTRPTPTT TVAPDTASWS AWGEWSACSQ PCGGCGTKTR
610 620 630 640 650
VRACYGGNQV CPGSNLDRES CNAHACAKPK KGMICNGRLL LPCDLLAKLN
660 670 680 690 700
FGSNNYLNPK LKQSGFARSS TLPLPRISQR KPVFVNELEV HPPTERFLSS
710 720 730 740 750
STRRVKRQTA NRFCEKRFIY QCPTALLTIQ MEYKPDTQGT NDAYFQQYPE
760
CCSGYTPRRG VCYKN
Note: No experimental confirmation available.
Length:765
Mass (Da):85,180
Last modified:October 1, 2002 - v2
Checksum:i8D9D9B43A584BA95
GO
Isoform b (identifier: Q93243-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     484-563: Missing.

Show »
Length:685
Mass (Da):76,533
Checksum:iE5460F26BF1C8244
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014343484 – 563Missing in isoform b. 1 PublicationAdd BLAST80

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY912076 mRNA. Translation: AAX81408.1.
Z78415 Genomic DNA. Translation: CAB01675.2.
Z78415 Genomic DNA. Translation: CAI58633.1.
PIRiT19366.
RefSeqiNP_001024413.1. NM_001029242.3. [Q93243-1]
NP_001024414.1. NM_001029243.4. [Q93243-2]
UniGeneiCel.5964.

Genome annotation databases

EnsemblMetazoaiC17G1.6a; C17G1.6a; WBGene00003553. [Q93243-1]
GeneIDi181208.
KEGGicel:CELE_C17G1.6.
UCSCiC17G1.6b. c. elegans. [Q93243-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY912076 mRNA. Translation: AAX81408.1.
Z78415 Genomic DNA. Translation: CAB01675.2.
Z78415 Genomic DNA. Translation: CAI58633.1.
PIRiT19366.
RefSeqiNP_001024413.1. NM_001029242.3. [Q93243-1]
NP_001024414.1. NM_001029243.4. [Q93243-2]
UniGeneiCel.5964.

3D structure databases

ProteinModelPortaliQ93243.
SMRiQ93243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C17G1.6a.

Protein family/group databases

MEROPSiM12.318.

Proteomic databases

EPDiQ93243.
PaxDbiQ93243.
PeptideAtlasiQ93243.
PRIDEiQ93243.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC17G1.6a; C17G1.6a; WBGene00003553. [Q93243-1]
GeneIDi181208.
KEGGicel:CELE_C17G1.6.
UCSCiC17G1.6b. c. elegans. [Q93243-1]

Organism-specific databases

CTDi181208.
WormBaseiC17G1.6a; CE31417; WBGene00003553; nas-37.
C17G1.6b; CE38034; WBGene00003553; nas-37.

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119227.
HOGENOMiHOG000016555.
InParanoidiQ93243.
OMAiYCETVEY.
OrthoDBiEOG091G0N5F.
PhylomeDBiQ93243.

Miscellaneous databases

PROiQ93243.

Gene expression databases

BgeeiWBGene00003553.

Family and domain databases

CDDicd00041. CUB. 1 hit.
Gene3Di2.60.120.290. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000859. CUB_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR017050. Metallopeptidase_nem.
IPR033955. NAS-37.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
IPR000884. TSP1_rpt.
[Graphical view]
PANTHERiPTHR10127:SF602. PTHR10127:SF602. 3 hits.
PfamiPF01400. Astacin. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
PIRSFiPIRSF036365. Astacin_nematoda. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 1 hit.
SM00209. TSP1. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01180. CUB. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50092. TSP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAS37_CAEEL
AccessioniPrimary (citable) accession number: Q93243
Secondary accession number(s): Q5CZ39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.