Q93235 (AT1B1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit beta-1

Alternative name(s):
Sodium/potassium-dependent ATPase subunit beta-1

Gene names

Name:	nkb-1
ORF Names:	C17E4.9

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane By similarity. May also may function in the peptidic vesicle transport pathway from the endoplasmic reticulum for the defecation behaviors. Ref.3
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma By similarity.
Subcellular location	Membrane; Single-pass type II membrane protein By similarity.
Disruption phenotype	Defecation defects. Ref.3
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro body morphogenesis Inferred from mutant phenotype. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase oogenesis Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase positive regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase receptor-mediated endocytosis Inferred from mutant phenotype. Source: WormBase
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sodium:potassium-exchanging ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Sodium/potassium-transporting ATPase subunit beta-1		PRO_0000250558
Regions
	Topological domain	1 – 51	51	Cytoplasmic Potential
	Transmembrane	52 – 72	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	73 – 320	248	Extracellular Potential
Amino acid modifications
	Glycosylation	140	1	N-linked (GlcNAc...) Ref.2 Ref.4 Ref.5
	Disulfide bond	176 ↔ 190		By similarity
	Disulfide bond	228 ↔ 285		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q93235 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: BB1CDDCBCD070B8E	FASTA	320	36,672
	10 20 30 40 50 60 MEKKVDENAT LMNGVETTGP ARDDVPETFR EFLYNKKNGT VMGRTGKSWF QIIVFYIIFY 70 80 90 100 110 120 AFLAAFWLTC LTIFMKTLDP KVPRFYGKGT IIGVNPGVGY QPWLKERPDS TLIKYNLRDQ 130 140 150 160 170 180 KSYKAYLEQM KTYLTKYDSN ATETRECGAG DSNDDLEKNP DALPCRFDLS VFDKGCSEKS 190 200 210 220 230 240 DFGYKSGKPC VIISLNRLIG WRPTDYQENS VPEEVKDRYK AGSIAINCRG ATNVDQEHIG 250 260 270 280 290 300 KVTYMPSNGI DGRYYPYVFT KGYQQPIAMV KFDTIPRNKL VIVECRAYAL NIEHDISSRL 310 320 GMVYFEVMVE DKPVEEKKEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins." Kaji H., Saito H., Yamauchi Y., Shinkawa T., Taoka M., Hirabayashi J., Kasai K., Takahashi N., Isobe T. Nat. Biotechnol. 21:667-672(2003) [PubMed: 12754521] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-140, MASS SPECTROMETRY. Strain: Bristol N2.
[3]	"A novel function of the Na+/K+ ATPase subunit in vesicle transport for the retrograde synaptic transmission pathway." Doi M., Iwasaki K. (In) Proceedings of the 14th international C. elegans meeting, pp.231-231, Los Angeles (2003) Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[4]	"Identification of the hydrophobic glycoproteins of Caenorhabditis elegans." Fan X., She Y.-M., Bagshaw R.D., Callahan J.W., Schachter H., Mahuran D.J. Glycobiology 15:952-964(2005) [PubMed: 15888633] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-140, MASS SPECTROMETRY.
[5]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-140, MASS SPECTROMETRY. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z81037 Genomic DNA. Translation: CAB02752.1.
PIR	T19358.
RefSeq	NP_492506.1. NM_060105.4.
UniGene	Cel.19226.
3D structure databases
ProteinModelPortal	Q93235.
SMR	Q93235. Positions 35-311.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C17E4.9.1; C17E4.9.1; C17E4.9. C17E4.9.2; C17E4.9.2; C17E4.9. C17E4.9.3; C17E4.9.3; C17E4.9.
GeneID	182726.
KEGG	cel:C17E4.9.
NMPDR	fig|6239.3.peg.2493.
UCSC	C17E4.9. c. elegans.
Organism-specific databases
CTD	182726.
WormBase	C17E4.9; CE08258; WBGene00007646; nkb-1.
Phylogenomic databases
eggNOG	meNOG09411.
GeneTree	EMGT00050000007260.
HOGENOM	HBG379014.
InParanoid	Q93235.
OMA	FTKGYQQ.
PhylomeDB	Q93235.
Gene expression databases
ArrayExpress	Q93235.
Family and domain databases
InterPro	IPR000402. ATPase_P-typ_cation-exchng_bsu. [Graphical view]
KO	K01540.
PANTHER	PTHR11523. ATPase_H_Na/K_b. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
PROSITE	PS00390. ATPASE_NA_K_BETA_1. 1 hit. PS00391. ATPASE_NA_K_BETA_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	918610.

Entry information

Entry name

AT1B1_CAEEL

Accession

Primary (citable) accession number: Q93235

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project