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Protein

Phosphoenolpyruvate-protein phosphotransferase

Gene

ptsI

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).By similarity

Miscellaneous

The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain.By similarity

Catalytic activityi

Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei191Tele-phosphohistidine intermediateBy similarity1
Binding sitei298PEPBy similarity1
Binding sitei334PEPBy similarity1
Metal bindingi433MagnesiumBy similarity1
Metal bindingi457MagnesiumBy similarity1
Binding sitei467PEPBy similarity1
Active sitei504Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciSAUR158878:G1G22-1180-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate-protein phosphotransferaseBy similarity (EC:2.7.3.9By similarity)
Alternative name(s):
Phosphotransferase system, enzyme IBy similarity
Gene namesi
Name:ptsI
Ordered Locus Names:SAV1084
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001470811 – 572Phosphoenolpyruvate-protein phosphotransferaseAdd BLAST572

Proteomic databases

PaxDbiQ931U2
PRIDEiQ931U2

2D gel databases

World-2DPAGEi0002:Q931U2

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi158878.SAV1084

Structurei

3D structure databases

ProteinModelPortaliQ931U2
SMRiQ931U2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni456 – 457PEP bindingBy similarity2

Domaini

The N-terminal domain contains the HPr binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site.By similarity

Sequence similaritiesi

Belongs to the PEP-utilizing enzyme family.Curated

Phylogenomic databases

eggNOGiENOG4105BZ3 Bacteria
COG1080 LUCA
HOGENOMiHOG000278513
KOiK08483
OMAiCNAEWAL
PhylomeDBiQ931U2

Family and domain databases

Gene3Di1.10.274.101 hit
InterProiView protein in InterPro
IPR008279 PEP-util_enz_mobile_dom
IPR018274 PEP_util_AS
IPR000121 PEP_util_C
IPR023151 PEP_util_CS
IPR036637 Phosphohistidine_dom_sf
IPR024692 PTS_EI
IPR006318 PTS_EI-like
IPR008731 PTS_EIN
IPR036618 PtsI_HPr-bd_sf
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PfamiView protein in Pfam
PF05524 PEP-utilisers_N, 1 hit
PF00391 PEP-utilizers, 1 hit
PF02896 PEP-utilizers_C, 1 hit
PIRSFiPIRSF000732 PTS_enzyme_I, 1 hit
SUPFAMiSSF47831 SSF47831, 1 hit
SSF51621 SSF51621, 1 hit
SSF52009 SSF52009, 1 hit
TIGRFAMsiTIGR01417 PTS_I_fam, 1 hit
PROSITEiView protein in PROSITE
PS00742 PEP_ENZYMES_2, 1 hit
PS00370 PEP_ENZYMES_PHOS_SITE, 1 hit

Sequencei

Sequence statusi: Complete.

Q931U2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLIKGIAA SDGVAIAKAY LLVEPDLTFD KNEKVTDVEG EVAKFNSAIE
60 70 80 90 100
ASKVELTKIR NNAEVQLGAD KAAIFDAHLL VLDDPELIQP IQDKIKNENA
110 120 130 140 150
NAATALTDVT TQFVTIFESM DNEYMKERAA DIRDVSKRVL SHILGVELPN
160 170 180 190 200
PSMIDESVVI VGNDLTPSDT AQLNKEFVQG FATNIGGRTS HSAIMSRSLE
210 220 230 240 250
IPAIVGTKSI TQEVKQGDII IVDGLNGDVI VNPTEDELIA YQDKRECYFA
260 270 280 290 300
DKKELQKLRD ADTVTVDGVH AELAANIGTP NDLPGVIENG AQGIGLYRTE
310 320 330 340 350
FLYMGRDQMP TEEEQFEAYK EVLEAMDGKR VVVRTLDIGG DKELSYLNLP
360 370 380 390 400
EEMNPFLGYR AIRLCLAQQD IFRPQLRALL RASVYGKLNI MFPMVATINE
410 420 430 440 450
FREAKAILLE EKENLKNEGH DISDDIELGI MVEIPATAAL ADVFAKEVDF
460 470 480 490 500
FSIGTNDLIQ YTLAADRMSE RVSYLYQPYN PSILRLVKQV IEASHKEGKW
510 520 530 540 550
TGMCGEMAGD ETAIPLLLGL GLDEFSMSAT SILKARRQIN GLSKNEMTEL
560 570
ANRAVDCATQ EEVIELVNNY VK
Length:572
Mass (Da):63,206
Last modified:December 1, 2001 - v1
Checksum:iF2AAE2D47B7853C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA Translation: BAB57246.1
RefSeqiWP_000040049.1, NC_002758.2

Genome annotation databases

EnsemblBacteriaiBAB57246; BAB57246; SAV1084
KEGGisav:SAV1084

Similar proteinsi

Entry informationi

Entry nameiPT1_STAAM
AccessioniPrimary (citable) accession number: Q931U2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: December 1, 2001
Last modified: February 28, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome