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Protein

GTP pyrophosphokinase

Gene

relA

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp (By similarity).By similarity

Catalytic activityi

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Pathway: ppGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GTP.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. GTP pyrophosphokinase (relA)
  2. no protein annotated in this organism
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GTP, the pathway ppGpp biosynthesis and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-1650-MONOMER.
UniPathwayiUPA00908; UER00884.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP pyrophosphokinase (EC:2.7.6.5)
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I
Gene namesi
Name:relA
Ordered Locus Names:SAV1634
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 736736GTP pyrophosphokinasePRO_0000166555Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV1634.

Structurei

3D structure databases

ProteinModelPortaliQ931Q4.
SMRiQ931Q4. Positions 15-380, 400-463, 658-736.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 156100HDAdd
BLAST
Domaini662 – 73675ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated
Contains 1 ACT domain.PROSITE-ProRule annotation
Contains 1 HD domain.Curated

Phylogenomic databases

eggNOGiCOG0317.
KOiK00951.
OMAiLDWLNFV.
OrthoDBiEOG6SV551.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q931Q4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGVYHIMNN EYPYSADEVL HKAKSYLSAD EYEYVLKSYH IAYEAHKGQF
60 70 80 90 100
RKNGLPYIMH PIQVAGILTE MRLDGPTIVA GFLHDVIEDT PYTFEDVKEM
110 120 130 140 150
FNEEVARIVD GVTKLKKVKY RSKEEQQAEN HRKLFIAIAK DVRVILVKLA
160 170 180 190 200
DRLHNMRTLK AMPREKQIRI SRETLEIYAP LAHHLGINTI KWELEDTALR
210 220 230 240 250
YIDNVQYFRI VNLMKKKRSE REAYIETAID RIRTEMDRMN IEGDINGRPK
260 270 280 290 300
HIYSIYRKMM KQKKQFDQIF DLLAIRVIVN SINDCYAILG LVHTLWKPMP
310 320 330 340 350
GRFKDYIAMP KQNLYQSLHT TVVGPNGDPL EIQIRTFDMH EIAEHGVAAH
360 370 380 390 400
WAYKEGKKVS EKDQTYQNKL NWLKELAEAD HTSSDAQEFM ETLKYDLQSD
410 420 430 440 450
KVYAFTPASD VIELPYGAVP IDFAYAIHSE VGNKMIGAKV NGKIVPIDYI
460 470 480 490 500
LQTGDIVEIR TSKHSYGPSR DWLKIVKSSS AKGKIKSFFK KQDRSSNIEK
510 520 530 540 550
GRMMVEVEIK EQGFRVEDIL TEKNIQVVNE KYNFANEDDL FAAVGFGGVT
560 570 580 590 600
SLQIVNKLTE RQRILDKQRA LNEEQEVTKS LPIKDNIITD SGVYVEGLEN
610 620 630 640 650
VLIKLSKCCN PIPGDDIVGY ITKGHGIKVH RTDCPNIKNE TERLINVEWV
660 670 680 690 700
KSKDATQKYQ VDLEVTAYDR NGLLNEVLQA VSSTAGNLIK VSGRSDIDKN
710 720 730
AIINISVMVK NVNDVYRVVE KIKQLGDVYT VTRVWN
Length:736
Mass (Da):84,576
Last modified:February 15, 2005 - v2
Checksum:iC158A507036972C1
GO

Sequence cautioni

The sequence BAB57796.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57796.1. Different initiation.
RefSeqiNP_372158.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB57796; BAB57796; SAV1634.
KEGGisav:SAV1634.
PATRICi19564018. VBIStaAur52173_1687.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57796.1. Different initiation.
RefSeqiNP_372158.1. NC_002758.2.

3D structure databases

ProteinModelPortaliQ931Q4.
SMRiQ931Q4. Positions 15-380, 400-463, 658-736.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV1634.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB57796; BAB57796; SAV1634.
KEGGisav:SAV1634.
PATRICi19564018. VBIStaAur52173_1687.

Phylogenomic databases

eggNOGiCOG0317.
KOiK00951.
OMAiLDWLNFV.
OrthoDBiEOG6SV551.

Enzyme and pathway databases

UniPathwayiUPA00908; UER00884.
BioCyciSAUR158878:GJJ5-1650-MONOMER.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mu50 / ATCC 700699.

Entry informationi

Entry nameiRELA_STAAM
AccessioniPrimary (citable) accession number: Q931Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: June 24, 2015
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.