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Q931P5

- THII_STAAM

UniProt

Q931P5 - THII_STAAM

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Protein
Probable tRNA sulfurtransferase
Gene
thiI, SAV1715
Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS By similarity.UniRule annotation

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei265 – 2651ATP By similarity
Binding sitei287 – 2871ATP; via amide nitrogen By similarity
Binding sitei296 – 2961ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi183 – 1842ATP By similarity
Nucleotide bindingi208 – 2092ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. sulfurtransferase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA thio-modification Source: UniProtKB-HAMAP
  2. thiamine biosynthetic process Source: UniProtKB-HAMAP
  3. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-1731-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferase (EC:2.8.1.4)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferase
Thiamine biosynthesis protein ThiI
tRNA 4-thiouridine synthase
Gene namesi
Name:thiI
Ordered Locus Names:SAV1715
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000002481: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 407407Probable tRNA sulfurtransferaseUniRule annotation
PRO_0000154864Add
BLAST

2D gel databases

World-2DPAGE0002:Q931P5.

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV1715.

Structurei

3D structure databases

ProteinModelPortaliQ931P5.
SMRiQ931P5. Positions 3-385.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini61 – 165105THUMP
Add
BLAST

Sequence similaritiesi

Belongs to the ThiI family.
Contains 1 THUMP domain.

Phylogenomic databases

eggNOGiCOG0301.
KOiK03151.
OMAiAQMKGPD.
OrthoDBiEOG6TBHGR.
PhylomeDBiQ931P5.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q931P5-1 [UniParc]FASTAAdd to Basket

« Hide

MKYDHLLVRY GELTLKGSNR KKFVNQLRNN VNKSLKGLDG FVVKGKRDRM    50
YIELEDHADI NEITYRLSKI FGIKSISPVL KVEKTIEAMS AAAIKFAQQF 100
EENSTFKIDV KRADKNFPMD TYELQRELGG TVLKQIENVS VNVKRPDHEI 150
RVEVRLDAIY MYEEVVPGSG GLPVGTGGKT LLMLSGGIDS PVAGMEVMRR 200
GVTIEAIHFH SPPFTSDQAK EKVIELTRIL AERVGPIKLH IVPFTELQKQ 250
VNKVVHPRYT MTSTRRMMMR VADKLVHQIG ALAIVNGENL GQVASQTLHS 300
MYAINNVTST PVLRPLLTYD KEEIIIKSKE IGTFETSIQP FEDCCTIFTP 350
KNPVTEPNFE KVVQYESVFD FEEMINRAVE NIETLEITSD YKTIKEQQTN 400
QLINDFL 407
Length:407
Mass (Da):46,228
Last modified:December 1, 2001 - v1
Checksum:i2BD404728AA854B7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000017 Genomic DNA. Translation: BAB57877.1.
RefSeqiNP_372239.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB57877; BAB57877; SAV1715.
GeneIDi1121690.
KEGGisav:SAV1715.
PATRICi19564182. VBIStaAur52173_1769.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000017 Genomic DNA. Translation: BAB57877.1 .
RefSeqi NP_372239.1. NC_002758.2.

3D structure databases

ProteinModelPortali Q931P5.
SMRi Q931P5. Positions 3-385.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 158878.SAV1715.

2D gel databases

World-2DPAGE 0002:Q931P5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB57877 ; BAB57877 ; SAV1715 .
GeneIDi 1121690.
KEGGi sav:SAV1715.
PATRICi 19564182. VBIStaAur52173_1769.

Phylogenomic databases

eggNOGi COG0301.
KOi K03151.
OMAi AQMKGPD.
OrthoDBi EOG6TBHGR.
PhylomeDBi Q931P5.

Enzyme and pathway databases

UniPathwayi UPA00060 .
BioCyci SAUR158878:GJJ5-1731-MONOMER.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
HAMAPi MF_00021. ThiI.
InterProi IPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view ]
Pfami PF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view ]
SMARTi SM00981. THUMP. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00342. TIGR00342. 1 hit.
PROSITEi PS51165. THUMP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mu50 / ATCC 700699.

Entry informationi

Entry nameiTHII_STAAM
AccessioniPrimary (citable) accession number: Q931P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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