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Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathway: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei265 – 2651ATPUniRule annotation
Binding sitei287 – 2871ATP; via amide nitrogenUniRule annotation
Binding sitei296 – 2961ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi183 – 1842ATPUniRule annotation
Nucleotide bindingi208 – 2092ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-1731-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:SAV1715
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 407407Probable tRNA sulfurtransferasePRO_0000154864Add
BLAST

2D gel databases

World-2DPAGE0002:Q931P5.

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV1715.

Structurei

3D structure databases

ProteinModelPortaliQ931P5.
SMRiQ931P5. Positions 3-385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini61 – 165105THUMPUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation
Contains 1 THUMP domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0301.
KOiK03151.
OMAiAQKIDTF.
OrthoDBiEOG6TBHGR.
PhylomeDBiQ931P5.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q931P5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYDHLLVRY GELTLKGSNR KKFVNQLRNN VNKSLKGLDG FVVKGKRDRM
60 70 80 90 100
YIELEDHADI NEITYRLSKI FGIKSISPVL KVEKTIEAMS AAAIKFAQQF
110 120 130 140 150
EENSTFKIDV KRADKNFPMD TYELQRELGG TVLKQIENVS VNVKRPDHEI
160 170 180 190 200
RVEVRLDAIY MYEEVVPGSG GLPVGTGGKT LLMLSGGIDS PVAGMEVMRR
210 220 230 240 250
GVTIEAIHFH SPPFTSDQAK EKVIELTRIL AERVGPIKLH IVPFTELQKQ
260 270 280 290 300
VNKVVHPRYT MTSTRRMMMR VADKLVHQIG ALAIVNGENL GQVASQTLHS
310 320 330 340 350
MYAINNVTST PVLRPLLTYD KEEIIIKSKE IGTFETSIQP FEDCCTIFTP
360 370 380 390 400
KNPVTEPNFE KVVQYESVFD FEEMINRAVE NIETLEITSD YKTIKEQQTN

QLINDFL
Length:407
Mass (Da):46,228
Last modified:December 1, 2001 - v1
Checksum:i2BD404728AA854B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57877.1.
RefSeqiNP_372239.1. NC_002758.2.
WP_000872662.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB57877; BAB57877; SAV1715.
KEGGisav:SAV1715.
PATRICi19564182. VBIStaAur52173_1769.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57877.1.
RefSeqiNP_372239.1. NC_002758.2.
WP_000872662.1. NC_002758.2.

3D structure databases

ProteinModelPortaliQ931P5.
SMRiQ931P5. Positions 3-385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV1715.

2D gel databases

World-2DPAGE0002:Q931P5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB57877; BAB57877; SAV1715.
KEGGisav:SAV1715.
PATRICi19564182. VBIStaAur52173_1769.

Phylogenomic databases

eggNOGiCOG0301.
KOiK03151.
OMAiAQKIDTF.
OrthoDBiEOG6TBHGR.
PhylomeDBiQ931P5.

Enzyme and pathway databases

UniPathwayiUPA00060.
BioCyciSAUR158878:GJJ5-1731-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mu50 / ATCC 700699.

Entry informationi

Entry nameiTHII_STAAM
AccessioniPrimary (citable) accession number: Q931P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: December 1, 2001
Last modified: June 24, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.