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Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

Catalytic activityi

ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei265ATPUniRule annotation1
Binding sitei287ATP; via amide nitrogenUniRule annotation1
Binding sitei296ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi183 – 184ATPUniRule annotation2
Nucleotide bindingi208 – 209ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase, tRNA-binding
Biological processThiamine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:SAV1715
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001548641 – 407Probable tRNA sulfurtransferaseAdd BLAST407

Proteomic databases

PaxDbiQ931P5.

2D gel databases

World-2DPAGEi0002:Q931P5.

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV1715.

Structurei

3D structure databases

ProteinModelPortaliQ931P5.
SMRiQ931P5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 165THUMPUniRule annotationAdd BLAST105

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D8I. Bacteria.
COG0301. LUCA.
KOiK03151.
OMAiKLFPEIM.
PhylomeDBiQ931P5.

Family and domain databases

CDDicd01712. ThiI. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI. 1 hit.
InterProiView protein in InterPro
IPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP_dom.
IPR003720. tRNA_STrfase.
PfamiView protein in Pfam
PF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
SMARTiView protein in SMART
SM00981. THUMP. 1 hit.
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiView protein in PROSITE
PS51165. THUMP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q931P5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYDHLLVRY GELTLKGSNR KKFVNQLRNN VNKSLKGLDG FVVKGKRDRM
60 70 80 90 100
YIELEDHADI NEITYRLSKI FGIKSISPVL KVEKTIEAMS AAAIKFAQQF
110 120 130 140 150
EENSTFKIDV KRADKNFPMD TYELQRELGG TVLKQIENVS VNVKRPDHEI
160 170 180 190 200
RVEVRLDAIY MYEEVVPGSG GLPVGTGGKT LLMLSGGIDS PVAGMEVMRR
210 220 230 240 250
GVTIEAIHFH SPPFTSDQAK EKVIELTRIL AERVGPIKLH IVPFTELQKQ
260 270 280 290 300
VNKVVHPRYT MTSTRRMMMR VADKLVHQIG ALAIVNGENL GQVASQTLHS
310 320 330 340 350
MYAINNVTST PVLRPLLTYD KEEIIIKSKE IGTFETSIQP FEDCCTIFTP
360 370 380 390 400
KNPVTEPNFE KVVQYESVFD FEEMINRAVE NIETLEITSD YKTIKEQQTN

QLINDFL
Length:407
Mass (Da):46,228
Last modified:December 1, 2001 - v1
Checksum:i2BD404728AA854B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57877.1.
RefSeqiWP_000872662.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB57877; BAB57877; SAV1715.
KEGGisav:SAV1715.

Similar proteinsi

Entry informationi

Entry nameiTHII_STAAM
AccessioniPrimary (citable) accession number: Q931P5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: December 1, 2001
Last modified: August 30, 2017
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families