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Q931P5

- THII_STAAM

UniProt

Q931P5 - THII_STAAM

Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

    Catalytic activityi

    L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei265 – 2651ATPUniRule annotation
    Binding sitei287 – 2871ATP; via amide nitrogenUniRule annotation
    Binding sitei296 – 2961ATPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi183 – 1842ATPUniRule annotation
    Nucleotide bindingi208 – 2092ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. sulfurtransferase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-HAMAP
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
    3. tRNA thio-modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Enzyme and pathway databases

    BioCyciSAUR158878:GJJ5-1731-MONOMER.
    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
    Alternative name(s):
    Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
    Thiamine biosynthesis protein ThiIUniRule annotation
    tRNA 4-thiouridine synthaseUniRule annotation
    Gene namesi
    Name:thiIUniRule annotation
    Ordered Locus Names:SAV1715
    OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
    Taxonomic identifieri158878 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000002481: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 407407Probable tRNA sulfurtransferasePRO_0000154864Add
    BLAST

    2D gel databases

    World-2DPAGE0002:Q931P5.

    Interactioni

    Protein-protein interaction databases

    STRINGi158878.SAV1715.

    Structurei

    3D structure databases

    ProteinModelPortaliQ931P5.
    SMRiQ931P5. Positions 3-385.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini61 – 165105THUMPUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ThiI family.UniRule annotation
    Contains 1 THUMP domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0301.
    KOiK03151.
    OMAiAQMKGPD.
    OrthoDBiEOG6TBHGR.
    PhylomeDBiQ931P5.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_00021. ThiI.
    InterProiIPR014729. Rossmann-like_a/b/a_fold.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view]
    PfamiPF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view]
    SMARTiSM00981. THUMP. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
    PROSITEiPS51165. THUMP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q931P5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKYDHLLVRY GELTLKGSNR KKFVNQLRNN VNKSLKGLDG FVVKGKRDRM    50
    YIELEDHADI NEITYRLSKI FGIKSISPVL KVEKTIEAMS AAAIKFAQQF 100
    EENSTFKIDV KRADKNFPMD TYELQRELGG TVLKQIENVS VNVKRPDHEI 150
    RVEVRLDAIY MYEEVVPGSG GLPVGTGGKT LLMLSGGIDS PVAGMEVMRR 200
    GVTIEAIHFH SPPFTSDQAK EKVIELTRIL AERVGPIKLH IVPFTELQKQ 250
    VNKVVHPRYT MTSTRRMMMR VADKLVHQIG ALAIVNGENL GQVASQTLHS 300
    MYAINNVTST PVLRPLLTYD KEEIIIKSKE IGTFETSIQP FEDCCTIFTP 350
    KNPVTEPNFE KVVQYESVFD FEEMINRAVE NIETLEITSD YKTIKEQQTN 400
    QLINDFL 407
    Length:407
    Mass (Da):46,228
    Last modified:December 1, 2001 - v1
    Checksum:i2BD404728AA854B7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000017 Genomic DNA. Translation: BAB57877.1.
    RefSeqiNP_372239.1. NC_002758.2.

    Genome annotation databases

    EnsemblBacteriaiBAB57877; BAB57877; SAV1715.
    GeneIDi1121690.
    KEGGisav:SAV1715.
    PATRICi19564182. VBIStaAur52173_1769.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000017 Genomic DNA. Translation: BAB57877.1 .
    RefSeqi NP_372239.1. NC_002758.2.

    3D structure databases

    ProteinModelPortali Q931P5.
    SMRi Q931P5. Positions 3-385.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 158878.SAV1715.

    2D gel databases

    World-2DPAGE 0002:Q931P5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB57877 ; BAB57877 ; SAV1715 .
    GeneIDi 1121690.
    KEGGi sav:SAV1715.
    PATRICi 19564182. VBIStaAur52173_1769.

    Phylogenomic databases

    eggNOGi COG0301.
    KOi K03151.
    OMAi AQMKGPD.
    OrthoDBi EOG6TBHGR.
    PhylomeDBi Q931P5.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .
    BioCyci SAUR158878:GJJ5-1731-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_00021. ThiI.
    InterProi IPR014729. Rossmann-like_a/b/a_fold.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view ]
    Pfami PF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view ]
    SMARTi SM00981. THUMP. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00342. TIGR00342. 1 hit.
    PROSITEi PS51165. THUMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Mu50 / ATCC 700699.

    Entry informationi

    Entry nameiTHII_STAAM
    AccessioniPrimary (citable) accession number: Q931P5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2002
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3