Iron-regulated surface determinant protein H precursor - Staphylococcus aureus (strain Mu50 / ATCC 700699)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Iron-regulated surface determinant protein H

Alternative name(s):
Haptoglobin receptor A
Staphylococcus aureus surface protein I

Gene names

Name:	isdH
Synonyms:	harA, sasI
Ordered Locus Names:	SAV1731

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcus ›

Protein attributes

Sequence length	891 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds human plasma haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin. Binds haptoglobin-hemoglobin complexes with significantly higher affinity than haptoglobin alone By similarity.
Subcellular location	Secreted › cell wall; Peptidoglycan-anchor Potential.
Domain	The NEAT 1 domain binds with higher affinity than the NEAT 2 domain haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin By similarity.
Sequence similarities	Belongs to the IsdH family. Contains 3 NEAT domains.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Repeat Signal
PTM	Peptidoglycan-anchor
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Cellular_component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 40

40

Potential

Chain

41 – 860

820

Iron-regulated surface determinant protein H

PRO_0000285187

Propeptide

861 – 891

31

Removed by sortase Potential

PRO_0000285188

Regions

Domain

105 – 232

128

NEAT 1

Domain

345 – 471

127

NEAT 2

Domain

543 – 660

118

NEAT 3

Motif

857 – 861

5

LPXTG sorting signal Potential

Compositional bias

698 – 775

78

Asp-rich

Amino acid modifications

Modified residue

860

1

Pentaglycyl murein peptidoglycan amidated threonine Potential

Secondary structure

1

.

891

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q931P4 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 7226A157FFE27123
Show »

FASTA

891

100,466

        10         20         30         40         50         60 
MNKHHPKLRS FYSIRKSTLG VASVIVSTLF LITSQHQAQA AENTNTSDKI SENQNNNATT 

        70         80         90        100        110        120 
TQQPKDTNQT QPATQPVITA KNYPAADESL KDAIKDPALE NKEHDIGPRE QVNFQLLDKN 

       130        140        150        160        170        180 
NETQYYHFFS IKDPADVYYT KKKAEVELDI NTASTWKKFE VYENNQKLPV RLVSYSPVPE 

       190        200        210        220        230        240 
DHAYIRFPVS DGTQELKIVS STQIDDGEET NYDYTKLVFA KPIYNDPSLV KSDTNDAVVT 

       250        260        270        280        290        300 
NDQSSSDASN QTNTNTSNQN TSTTNNANNQ PQATTNMSQP AQPKSSANAD QASSQPAHET 

       310        320        330        340        350        360 
NSNGNTNDKT NESSNQSDVN QQYPPADESL QDAIKNPAII DKEHTADNWR PIDFQMKNDK 

       370        380        390        400        410        420 
GERQFYHYAS TVEPATVIFT KTGPVIELGL KTASTWKKFE VYEGDKKLPV ELVSYDSDKD 

       430        440        450        460        470        480 
YAYIRFPVSN GTRDVKIVSS IEYGENIHED YDYTLMVFAQ PITNNPDDYV DEETYNLQKL 

       490        500        510        520        530        540 
LAPYHKAKTL ERQVYELEKL QEKLPEKYKA EYKKKLDQTR VELADQVKSA VTEFENVTPT 

       550        560        570        580        590        600 
NDQLTDLQEA HFVVFESEEN SESVMDGFVE HPFYTATLNG QKYVVMKTKD DSYWKDLIVE 

       610        620        630        640        650        660 
GKRVTTVSKD PKNNSRTLIF PYIPDKAVYN AIVKVVVANI GYEGQYHVRI INQDINTKDD 

       670        680        690        700        710        720 
DTSQNNTSEP LNVQTGQEGK VADTDVAENS STATNPKDAS DKADVIEPES DVVKDADNNI 

       730        740        750        760        770        780 
DKDVQHDVDH LSDMSDNNHF DKYDLKEMDT QIAKDTDRNV DNSVGMSSNV DTDKDSNKNK 

       790        800        810        820        830        840 
DKVIQLAHIA DKNNHTGKAA KLDVVKQNYN NTDKVTDKKT TEHLPSDIHK TVDKTVKTKE 

       850        860        870        880        890 
KAGTPSKENK LSQSKMLPKT GETTSSQSWW GLYALLGMLA LFIPKFRKES K

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References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus."
Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.

Hiramatsu K.
Lancet 357:1225-1240(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Mu50 / ATCC 700699.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000017 Genomic DNA. Translation: BAB57893.1.

RefSeq

NP_372255.1. NC_002758.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2E7D	X-ray	2.20	A/B	539-664	[»]
2Z6F	X-ray	1.90	A	539-664	[»]
3QUG	X-ray	1.70	A/B	539-664	[»]
3QUH	X-ray	2.70	A/B	539-664	[»]

ProteinModelPortal

Q931P4.

SMR

Q931P4. Positions 86-227, 326-466, 543-655.

ModBase

Search...

Protein-protein interaction databases

STRING

158878.SAV1731.

Protein family/group databases

TCDB

9.A.39.1.2. gram-positive bacterial hemoglobin receptor (Isd) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAB57893; BAB57893; SAV1731.

GeneID

1121706.

KEGG

sav:SAV1731.

PATRIC

19564216. VBIStaAur52173_1786.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

NOG12793.

HOGENOM

HOG000280077.

OMA

VQTGQED.

ProtClustDB

CLSK885152.

Enzyme and pathway databases

BioCyc

SAUR158878:GJJ5-1747-MONOMER.

Family and domain databases

InterPro

IPR005877. Gpos_YSIRK.
IPR019930. Haptoglobin-bd_HarA.
IPR019931. LPXTG_anchor.
IPR006635. NEA_transpt.
[Graphical view]

Pfam

PF05031. NEAT. 3 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]

SMART

SM00725. NEAT. 3 hits.
[Graphical view]

TIGRFAMs

TIGR03658. IsdH_HarA. 1 hit.
TIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.

PROSITE

PS50847. GRAM_POS_ANCHORING. False negative.
PS50978. NEAT. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q931P4.

Entry information

Entry name

ISDH_STAAM

Accession

Primary (citable) accession number: Q931P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	December 1, 2001
Last modified:	May 29, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families