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Protein

PTS system lactose-specific EIICB component

Gene

lacE

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II LacEF PTS system is involved in lactose transport.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei474Phosphocysteine intermediate; for EIIB activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system lactose-specific EIICB componentBy similarity
Alternative name(s):
EIICB-LacBy similarity
Short name:
EII-LacBy similarity
Including the following 2 domains:
PTS system lactose-specific EIIC componentBy similarity
Alternative name(s):
Lactose permease IIC componentBy similarity
PTS system lactose-specific EIIB componentBy similarity (EC:2.7.1.207By similarity)
Alternative name(s):
Lactose-specific phosphotransferase enzyme IIB componentBy similarity
Gene namesi
Name:lacEBy similarity
Ordered Locus Names:SAV2190
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

Subcellular locationi

Q931G6:
  • Cell membrane PROSITE-ProRule annotationBy similarity; Multi-pass membrane protein PROSITE-ProRule annotationBy similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei31 – 51HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei65 – 85HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei104 – 124HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei133 – 153HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei178 – 198HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei223 – 243HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei283 – 303HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei340 – 360HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei382 – 402HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865861 – 570PTS system lactose-specific EIICB componentAdd BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei474Phosphocysteine; by EIIAPROSITE-ProRule annotationBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ931G6.

Expressioni

Inductioni

Induced by lactose, galactose and galactose-6-P. Repressed by glucose.By similarity

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV2190.

Structurei

3D structure databases

ProteinModelPortaliQ931G6.
SMRiQ931G6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 410PTS EIIC type-3PROSITE-ProRule annotationAdd BLAST402
Domaini467 – 570PTS EIIB type-3PROSITE-ProRule annotationAdd BLAST104

Domaini

The EIIC type-3 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation
The PTS EIIB type-3 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CK1. Bacteria.
COG1455. LUCA.
HOGENOMiHOG000235576.
KOiK02787.
K02788.
OMAiISAMPFM.
PhylomeDBiQ931G6.

Family and domain databases

InterProiView protein in InterPro
IPR036095. PTS_EIIB-like_sf.
IPR003501. PTS_EIIB_2/3.
IPR013012. PTS_EIIB_3.
IPR003352. PTS_EIIC.
IPR004501. PTS_EIIC_3.
IPR004801. PTS_IIC_lac_spec.
PfamiView protein in Pfam
PF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00394. lac_pts_IIC. 1 hit.
TIGR00410. lacE. 1 hit.
TIGR00853. pts-lac. 1 hit.
PROSITEiView protein in PROSITE
PS51100. PTS_EIIB_TYPE_3. 1 hit.
PS51105. PTS_EIIC_TYPE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Q931G6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMQKLIAQIE KGKPFFEKLS RNIYLRAIRD GFISAMPVIL FSSIFLLIAY
60 70 80 90 100
VPNIFGFKWD KGMEAILMKP YNYTMGLVAF LVAGTTAKSL TDSFNRKLES
110 120 130 140 150
TNQINFISTM LAAMCGFLFL ASDPAKDGGF LSAFMGTKGL LTAFLSAFVT
160 170 180 190 200
VIVYNFCVKR NITIKMPKEV PPNISQVFKD LIPFSAVIII LYALDLVIRN
210 220 230 240 250
SFKSNVAEGI LKLFEPLFTA ADGWIGVTII FGAFALFWFV GIHGPSIVEP
260 270 280 290 300
AIAAITYANI EANFKLLQAG EHADKIITSG TQMFIVTFGG TGATLVVPFM
310 320 330 340 350
FMWMTKSKRN KAIGRASVVP TFFGVNEPIL FGAPLVLNPV FFIPFVLAPI
360 370 380 390 400
VNVWIFKLFV EVLGMNSFSV NLPWTTPGPL GIIMGTGFGL WSFVLAITLI
410 420 430 440 450
VVDIIIYYPF LKVYDSEILD EEEGRKESNS DLKEKVAANF DTKKADSILA
460 470 480 490 500
ASGVSDDAAK ASNITEQTNV LVLCAGGGTS GLLANALNKA AEEYHVPVKA
510 520 530 540 550
AAGGYGAHMD IMKEYQLIIL APQVASNYED IKQDTDRLGI KLAKTQGAEY
560 570
IKLTRDGQAA LDFVQQQFEN
Length:570
Mass (Da):62,432
Last modified:December 1, 2001 - v1
Checksum:i587192287079BE58
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB58352.1.
RefSeqiWP_000983330.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB58352; BAB58352; SAV2190.
GeneIDi31214932.
KEGGisav:SAV2190.

Similar proteinsi

Entry informationi

Entry nameiPTLCB_STAAM
AccessioniPrimary (citable) accession number: Q931G6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 1, 2001
Last modified: October 25, 2017
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome