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Protein

Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial

Gene

osgl-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (By similarity). Involved in mitochondrial genome maintenance.UniRule annotation1 Publication

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

a divalent metal cationUniRule annotationNote: Binds 1 divalent metal cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi133Divalent metal cationUniRule annotation1
Metal bindingi137Divalent metal cationUniRule annotation1
Binding sitei189SubstrateUniRule annotation1
Binding sitei208Substrate; via amide nitrogenUniRule annotation1
Binding sitei212SubstrateUniRule annotation1
Binding sitei336SubstrateUniRule annotation1
Metal bindingi337Divalent metal cationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processtRNA processing
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrialUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein osgl-1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein osgl-1UniRule annotation
Gene namesi
Name:osgl-1UniRule annotation
ORF Names:C01G10.10
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiC01G10.10a ; CE20480 ; WBGene00007237 ; osgl-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Enhances longevity, resistance to oxidative stress, and perturbs mitochondrial reticulum morphology.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 22MitochondrionUniRule annotationAdd BLAST22
ChainiPRO_000042453523 – 421Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrialAdd BLAST399

Proteomic databases

EPDiQ93170
PaxDbiQ93170
PeptideAtlasiQ93170

Expressioni

Gene expression databases

BgeeiWBGene00007237

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi6239.C01G10.10

Structurei

3D structure databases

ProteinModelPortaliQ93170
SMRiQ93170
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni156 – 160Substrate bindingUniRule annotation5
Regioni308 – 309Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2707 Eukaryota
COG0533 LUCA
GeneTreeiENSGT00550000075001
HOGENOMiHOG000019493
InParanoidiQ93170
KOiK01409
OMAiANRMEHA
OrthoDBiEOG091G0HJR
PhylomeDBiQ93170

Family and domain databases

HAMAPiMF_01445 TsaD, 1 hit
InterProiView protein in InterPro
IPR000905 Gcp-like_dom
IPR017861 KAE1/TsaD
IPR022450 TsaD
PANTHERiPTHR11735 PTHR11735, 1 hit
PTHR11735:SF6 PTHR11735:SF6, 1 hit
PfamiView protein in Pfam
PF00814 Peptidase_M22, 1 hit
PRINTSiPR00789 OSIALOPTASE
TIGRFAMsiTIGR00329 gcp_kae1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93170-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIPKILNNN LVLKRIFCRN YSVKVLGIET SCDDTAVAIV NEKREILSSE
60 70 80 90 100
RYTERAIQRQ QGGINPSVCA LQHRENLPRL IEKCLNDAGT SPKDLDAVAV
110 120 130 140 150
TVTPGLVIAL KEGISAAIGF AKKHRLPLIP VHHMRAHALS ILLVDDSVRF
160 170 180 190 200
PFSAVLLSGG HALISVAEDV EKFKLYGQSV SGSPGECIDK VARQLGDLGS
210 220 230 240 250
EFDGIHVGAA VEILASRASA DGHLRYPIFL PNVPKANMNF DQIKGSYLNL
260 270 280 290 300
LERLRKNSET SIDIPDFCAS LQNTVARHIS SKLHIFFESL SEQEKLPKQL
310 320 330 340 350
VIGGGVAANQ YIFGAISKLS AAHNVTTIKV LLSLCTDNAE MIAYSGLLML
360 370 380 390 400
VNRSEAIWWR PNDIPDTIYA HARSDIGTDA SSEIIDTPRR KLVTSTIHGT
410 420
ERIRFRNLDD FKKPKSPKTT E
Length:421
Mass (Da):46,276
Last modified:May 1, 1999 - v2
Checksum:iE084339219539C5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81030 Genomic DNA Translation: CAB02716.1
PIRiT18825
RefSeqiNP_506713.1, NM_074312.4
UniGeneiCel.9389

Genome annotation databases

EnsemblMetazoaiC01G10.10a; C01G10.10a; WBGene00007237
GeneIDi180015
KEGGicel:CELE_C01G10.10
UCSCiC01G10.10 c. elegans

Similar proteinsi

Entry informationi

Entry nameiOSGP2_CAEEL
AccessioniPrimary (citable) accession number: Q93170
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: May 1, 1999
Last modified: April 25, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health