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Protein

Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial

Gene

osgl-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (By similarity). Involved in mitochondrial genome maintenance.UniRule annotation1 Publication

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

a divalent metal cationUniRule annotationNote: Binds 1 divalent metal cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi133 – 1331Divalent metal cationUniRule annotation
Metal bindingi137 – 1371Divalent metal cationUniRule annotation
Binding sitei189 – 1891SubstrateUniRule annotation
Binding sitei208 – 2081Substrate; via amide nitrogenUniRule annotation
Binding sitei212 – 2121SubstrateUniRule annotation
Binding sitei336 – 3361SubstrateUniRule annotation
Metal bindingi337 – 3371Divalent metal cationUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrialUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein osgl-1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein osgl-1UniRule annotation
Gene namesi
Name:osgl-1UniRule annotation
ORF Names:C01G10.10
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiC01G10.10a; CE20480; WBGene00007237; osgl-1.

Subcellular locationi

  • Mitochondrion UniRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Enhances longevity, resistance to oxidative stress, and perturbs mitochondrial reticulum morphology.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2222MitochondrionUniRule annotationAdd
BLAST
Chaini23 – 421399Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrialPRO_0000424535Add
BLAST

Proteomic databases

EPDiQ93170.
PaxDbiQ93170.
PRIDEiQ93170.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi6239.C01G10.10.

Structurei

3D structure databases

ProteinModelPortaliQ93170.
SMRiQ93170. Positions 23-348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni156 – 1605Substrate bindingUniRule annotation
Regioni308 – 3092Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2707. Eukaryota.
COG0533. LUCA.
GeneTreeiENSGT00550000075001.
HOGENOMiHOG000019493.
InParanoidiQ93170.
KOiK01409.
OMAiIASEAHE.
OrthoDBiEOG761BVX.
PhylomeDBiQ93170.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93170-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIPKILNNN LVLKRIFCRN YSVKVLGIET SCDDTAVAIV NEKREILSSE
60 70 80 90 100
RYTERAIQRQ QGGINPSVCA LQHRENLPRL IEKCLNDAGT SPKDLDAVAV
110 120 130 140 150
TVTPGLVIAL KEGISAAIGF AKKHRLPLIP VHHMRAHALS ILLVDDSVRF
160 170 180 190 200
PFSAVLLSGG HALISVAEDV EKFKLYGQSV SGSPGECIDK VARQLGDLGS
210 220 230 240 250
EFDGIHVGAA VEILASRASA DGHLRYPIFL PNVPKANMNF DQIKGSYLNL
260 270 280 290 300
LERLRKNSET SIDIPDFCAS LQNTVARHIS SKLHIFFESL SEQEKLPKQL
310 320 330 340 350
VIGGGVAANQ YIFGAISKLS AAHNVTTIKV LLSLCTDNAE MIAYSGLLML
360 370 380 390 400
VNRSEAIWWR PNDIPDTIYA HARSDIGTDA SSEIIDTPRR KLVTSTIHGT
410 420
ERIRFRNLDD FKKPKSPKTT E
Length:421
Mass (Da):46,276
Last modified:May 1, 1999 - v2
Checksum:iE084339219539C5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81030 Genomic DNA. Translation: CAB02716.1.
PIRiT18825.
RefSeqiNP_506713.1. NM_074312.4.
UniGeneiCel.9389.

Genome annotation databases

EnsemblMetazoaiC01G10.10a; C01G10.10a; WBGene00007237.
GeneIDi180015.
KEGGicel:CELE_C01G10.10.
UCSCiC01G10.10. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81030 Genomic DNA. Translation: CAB02716.1.
PIRiT18825.
RefSeqiNP_506713.1. NM_074312.4.
UniGeneiCel.9389.

3D structure databases

ProteinModelPortaliQ93170.
SMRiQ93170. Positions 23-348.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C01G10.10.

Proteomic databases

EPDiQ93170.
PaxDbiQ93170.
PRIDEiQ93170.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC01G10.10a; C01G10.10a; WBGene00007237.
GeneIDi180015.
KEGGicel:CELE_C01G10.10.
UCSCiC01G10.10. c. elegans.

Organism-specific databases

CTDi180015.
WormBaseiC01G10.10a; CE20480; WBGene00007237; osgl-1.

Phylogenomic databases

eggNOGiKOG2707. Eukaryota.
COG0533. LUCA.
GeneTreeiENSGT00550000075001.
HOGENOMiHOG000019493.
InParanoidiQ93170.
KOiK01409.
OMAiIASEAHE.
OrthoDBiEOG761BVX.
PhylomeDBiQ93170.

Miscellaneous databases

NextBioi907756.
PROiQ93170.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Qri7/OSGEPL, the mitochondrial version of the universal Kae1/YgjD protein, is essential for mitochondrial genome maintenance."
    Oberto J., Breuil N., Hecker A., Farina F., Brochier-Armanet C., Culetto E., Forterre P.
    Nucleic Acids Res. 37:5343-5352(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MITOCHONDRIAL GENOME MAINTENANCE, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiOSGP2_CAEEL
AccessioniPrimary (citable) accession number: Q93170
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: May 1, 1999
Last modified: May 11, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.