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Q93113

- GST1D_ANOGA

UniProt

Q93113 - GST1D_ANOGA

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Protein

Glutathione S-transferase 1, isoform D

Gene
GstD1, GST1a, AGAP004164
Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has DDT dehydrochlorinase activity.2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane = 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride.1 Publication

Enzyme regulationi

Inhibited by S-hexylglutathione.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91Glutathione

GO - Molecular functioni

  1. DDT-dehydrochlorinase activity Source: UniProtKB-EC
  2. glutathione transferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase 1, isoform D (EC:2.5.1.18, EC:4.5.1.1)
Alternative name(s):
AgGst1-alpha
Aggst1-1
Aggst1-6
Aggst2-1
DDT-dehydrochlorinase
GST class-theta
Gene namesi
Name:GstD1
Synonyms:GST1a
ORF Names:AGAP004164
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
ProteomesiUP000007062: Chromosome 2R

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209Glutathione S-transferase 1, isoform DPRO_0000185962Add
BLAST

Expressioni

Developmental stagei

Expressed in larvae.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
209
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 54
Helixi10 – 2112
Beta strandi27 – 304
Helixi33 – 353
Helixi37 – 393
Helixi41 – 466
Beta strandi52 – 576
Beta strandi60 – 645
Helixi65 – 7612
Helixi87 – 10216
Helixi104 – 11815
Helixi124 – 14017
Turni141 – 1433
Beta strandi149 – 1513
Helixi154 – 16916
Helixi177 – 18913
Helixi193 – 20715

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PN9X-ray2.00A/B1-209[»]
ProteinModelPortaliQ93113.
SMRiQ93113. Positions 1-209.

Miscellaneous databases

EvolutionaryTraceiQ93113.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 8080GST N-terminalAdd
BLAST
Domaini86 – 207122GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 523Glutathione binding
Regioni64 – 663Glutathione binding

Sequence similaritiesi

Belongs to the GST superfamily. Theta family.

Phylogenomic databases

HOGENOMiHOG000125741.
KOiK00799.
OMAiAMNESID.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform D (identifier: Q93113-1) [UniParc]FASTAAdd to Basket

Also known as: 1-1, 1-6, 2-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDFYYLPGSA PCRAVQMTAA AVGVELNLKL TDLMKGEHMK PEFLKLNPQH    50
CIPTLVDNGF ALWESRAIQI YLAEKYGKDD KLYPKDPQKR AVVNQRLYFD 100
MGTLYQRFAD YHYPQIFAKQ PANPENEKKM KDAVGFLNTF LEGQEYAAGN 150
DLTIADLSLA ATIATYEVAG FDFAPYPNVA AWFARCKANA PGYALNQAGA 200
DEFKAKFLS 209
Length:209
Mass (Da):23,445
Last modified:February 1, 1997 - v1
Checksum:i3B262EEE21FAA9D0
GO
Isoform A (identifier: O77462-1) [UniParc]FASTAAdd to Basket

Also known as: 1-3

The sequence of this isoform can be found in the external entry O77462.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:186
Mass (Da):21,255
GO
Isoform B (identifier: O77473-1) [UniParc]FASTAAdd to Basket

Also known as: 1-4

The sequence of this isoform can be found in the external entry O77473.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:216
Mass (Da):24,492
GO
Isoform C (identifier: Q93112-1) [UniParc]FASTAAdd to Basket

Also known as: 1-5

The sequence of this isoform can be found in the external entry Q93112.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:209
Mass (Da):23,783
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241V → A in CAA96105. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71481 mRNA. Translation: CAA96105.1.
Z81292 mRNA. Translation: CAB03593.1.
AF071160 Genomic DNA. Translation: AAC79995.1.
AAAB01008880 Genomic DNA. Translation: EAA44713.3.
RefSeqiXP_313050.3. XM_313050.4.

Genome annotation databases

EnsemblMetazoaiAGAP004164-RB; AGAP004164-PB; AGAP004164. [Q93113-1]
GeneIDi1273988.
KEGGiaga:AgaP_AGAP004164.
VectorBaseiAGAP004163. Anopheles gambiae.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71481 mRNA. Translation: CAA96105.1 .
Z81292 mRNA. Translation: CAB03593.1 .
AF071160 Genomic DNA. Translation: AAC79995.1 .
AAAB01008880 Genomic DNA. Translation: EAA44713.3 .
RefSeqi XP_313050.3. XM_313050.4.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PN9 X-ray 2.00 A/B 1-209 [» ]
ProteinModelPortali Q93113.
SMRi Q93113. Positions 1-209.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai AGAP004164-RB ; AGAP004164-PB ; AGAP004164 . [Q93113-1 ]
GeneIDi 1273988.
KEGGi aga:AgaP_AGAP004164.
VectorBasei AGAP004163. Anopheles gambiae.

Organism-specific databases

CTDi 1273988.

Phylogenomic databases

HOGENOMi HOG000125741.
KOi K00799.
OMAi AMNESID.

Miscellaneous databases

EvolutionaryTracei Q93113.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and localization of a glutathione S-transferase class I gene from Anopheles gambiae."
    Ranson H., Cornel A.J., Fournier D., Vaughan A., Collins F.H., Hemingway J.
    J. Biol. Chem. 272:5464-5468(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
    Strain: G3 and Zands.
    Tissue: Larva.
  2. "The role of alternative mRNA splicing in generating heterogeneity within the Anopheles gambiae class I glutathione S-transferase family."
    Ranson H., Collins F.H., Hemingway J.
    Proc. Natl. Acad. Sci. U.S.A. 95:14284-14289(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: ZAN/U.
  3. "The genome sequence of the malaria mosquito Anopheles gambiae."
    Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F.
    , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L.
    Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PEST.
  4. "Cloning and characterization of two glutathione S-transferases from a DDT-resistant strain of Anopheles gambiae."
    Ranson H., Prapanthadara L., Hemingway J.
    Biochem. J. 324:97-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
    Strain: Zands.
  5. "Structure of an insect delta-class glutathione S-transferase from a DDT-resistant strain of the malaria vector Anopheles gambiae."
    Chen L., Hall P.R., Zhou X.E., Ranson H., Hemingway J., Meehan E.J.
    Acta Crystallogr. D 59:2211-2217(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH S-HEXYLGLUTATHIONE INHIBITOR, ENZYME REGULATION, SUBUNIT.

Entry informationi

Entry nameiGST1D_ANOGA
AccessioniPrimary (citable) accession number: Q93113
Secondary accession number(s): Q7PH24, Q94998
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 3, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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