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Protein

Glutathione S-transferase 1, isoform D

Gene

GstD1

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has DDT dehydrochlorinase activity.2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane = 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride.1 Publication

Enzyme regulationi

Inhibited by S-hexylglutathione.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9Glutathione1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Lyase, Transferase

Enzyme and pathway databases

BRENDAi2.5.1.18. 358.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase 1, isoform D (EC:2.5.1.18, EC:4.5.1.1)
Alternative name(s):
AgGst1-alpha
Aggst1-1
Aggst1-6
Aggst2-1
DDT-dehydrochlorinase
GST class-theta
Gene namesi
Name:GstD1
Synonyms:GST1a
ORF Names:AGAP004164
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
Proteomesi
  • UP000007062 Componentsi: Chromosome 2R, Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001859621 – 209Glutathione S-transferase 1, isoform DAdd BLAST209

Expressioni

Developmental stagei

Expressed in larvae.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1209
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi10 – 21Combined sources12
Beta strandi27 – 30Combined sources4
Helixi33 – 35Combined sources3
Helixi37 – 39Combined sources3
Helixi41 – 46Combined sources6
Beta strandi52 – 57Combined sources6
Beta strandi60 – 64Combined sources5
Helixi65 – 76Combined sources12
Helixi87 – 102Combined sources16
Helixi104 – 118Combined sources15
Helixi124 – 140Combined sources17
Turni141 – 143Combined sources3
Beta strandi149 – 151Combined sources3
Helixi154 – 169Combined sources16
Helixi177 – 189Combined sources13
Helixi193 – 207Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PN9X-ray2.00A/B1-209[»]
ProteinModelPortaliQ93113.
SMRiQ93113.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ93113.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 80GST N-terminalAdd BLAST80
Domaini86 – 207GST C-terminalAdd BLAST122

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 52Glutathione binding3
Regioni64 – 66Glutathione binding3

Sequence similaritiesi

Belongs to the GST superfamily. Theta family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

HOGENOMiHOG000125741.
OrthoDBiEOG091G0MS3.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform D (identifier: Q93113-1) [UniParc]FASTAAdd to basket
Also known as: 1-1, 1-6, 2-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDFYYLPGSA PCRAVQMTAA AVGVELNLKL TDLMKGEHMK PEFLKLNPQH
60 70 80 90 100
CIPTLVDNGF ALWESRAIQI YLAEKYGKDD KLYPKDPQKR AVVNQRLYFD
110 120 130 140 150
MGTLYQRFAD YHYPQIFAKQ PANPENEKKM KDAVGFLNTF LEGQEYAAGN
160 170 180 190 200
DLTIADLSLA ATIATYEVAG FDFAPYPNVA AWFARCKANA PGYALNQAGA

DEFKAKFLS
Length:209
Mass (Da):23,445
Last modified:February 1, 1997 - v1
Checksum:i3B262EEE21FAA9D0
GO
Isoform A (identifier: O77462-1) [UniParc]FASTAAdd to basket
Also known as: 1-3
The sequence of this isoform can be found in the external entry O77462.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:186
Mass (Da):21,255
GO
Isoform B (identifier: O77473-1) [UniParc]FASTAAdd to basket
Also known as: 1-4
The sequence of this isoform can be found in the external entry O77473.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:216
Mass (Da):24,492
GO
Isoform C (identifier: Q93112-1) [UniParc]FASTAAdd to basket
Also known as: 1-5
The sequence of this isoform can be found in the external entry Q93112.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:209
Mass (Da):23,783
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24V → A in CAA96105 (PubMed:9038148).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71481 mRNA. Translation: CAA96105.1.
Z81292 mRNA. Translation: CAB03593.1.
AF071160 Genomic DNA. Translation: AAC79995.1.
AAAB01008880 Genomic DNA. Translation: EAA44713.3.
RefSeqiXP_313050.3. XM_313050.4.

Genome annotation databases

EnsemblMetazoaiAGAP004164-RB; AGAP004164-PB; AGAP004164. [Q93113-1]
GeneIDi1273988.
VectorBaseiAGAP004164-RB; AGAP004164-PB; AGAP004164.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71481 mRNA. Translation: CAA96105.1.
Z81292 mRNA. Translation: CAB03593.1.
AF071160 Genomic DNA. Translation: AAC79995.1.
AAAB01008880 Genomic DNA. Translation: EAA44713.3.
RefSeqiXP_313050.3. XM_313050.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PN9X-ray2.00A/B1-209[»]
ProteinModelPortaliQ93113.
SMRiQ93113.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAGAP004164-RB; AGAP004164-PB; AGAP004164. [Q93113-1]
GeneIDi1273988.
VectorBaseiAGAP004164-RB; AGAP004164-PB; AGAP004164.

Organism-specific databases

CTDi1273988.

Phylogenomic databases

HOGENOMiHOG000125741.
OrthoDBiEOG091G0MS3.

Enzyme and pathway databases

BRENDAi2.5.1.18. 358.

Miscellaneous databases

EvolutionaryTraceiQ93113.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGST1D_ANOGA
AccessioniPrimary (citable) accession number: Q93113
Secondary accession number(s): Q7PH24, Q94998
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.