Reviewed, UniProtKB/Swiss-Prot Q93112 (GST1C_ANOGA)

Names and origin

Protein names

Recommended name:
    Glutathione S-transferase 1, isoform C
    EC=2.5.1.18
Alternative name(s):
    GST class-theta
    AgGst1-alpha
    Aggst1-5

Gene names

Name:	GstD1
Synonyms:	GST1a
ORF Names:	AGAP004164

Organism

Anopheles gambiae (African malaria mosquito) [Complete proteome]

Taxonomic identifier

7165 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Anophelinae › Anopheles

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Enzyme regulation	Inhibited by S-hexylglutathione By similarity.
Subunit structure	Homodimer. Binds one molecule of S-hexylglutathione inhibitor per subunit By similarity.
Sequence similarities	Belongs to the GST superfamily. Theta family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform C (identifier: Q93112-1)

Also known as: 1-5;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: O77462-1)

Also known as: 1-3;

The sequence of this isoform can be found in the external entry O77462-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform B (identifier: O77473-1)

Also known as: 1-4;

The sequence of this isoform can be found in the external entry O77473-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform D (identifier: Q93113-1)

Also known as: 1-1; 1-6; 2-1;

The sequence of this isoform can be found in the external entry Q93113-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Glutathione S-transferase 1, isoform C		PRO_0000185961
Regions
	Domain	1 – 80	80	GST N-terminal
	Domain	86 – 207	122	GST C-terminal
	Region	6 – 11	6	S-hexylglutathione-binding By similarity
	Region	50 – 53	4	S-hexylglutathione-binding By similarity
	Region	64 – 66	3	S-hexylglutathione-binding By similarity
	Region	203 – 207	5	S-hexylglutathione-binding By similarity
Sites
	Active site	9	1	By similarity
	Binding site	33	1	S-hexylglutathione By similarity
	Binding site	38	1	S-hexylglutathione By similarity
	Binding site	101	1	S-hexylglutathione By similarity
	Binding site	105	1	S-hexylglutathione By similarity
	Binding site	113	1	S-hexylglutathione By similarity
Experimental info
	Sequence conflict	135	1	G → D in CAB03592. Ref.1
	Sequence conflict	139	1	S → T in CAB03592. Ref.1
	Sequence conflict	181	1	A → V in CAB03592. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform C (1-5) [UniParc]. Last modified May 16, 2006. Version 2. Checksum: ED01B322C2715409	FASTA	209	23,783
	10 20 30 40 50 60 MDFYYLPGSA PCRAVQMTAA AVGVELNLKL TDLMKGEHMK PEFLKINPQH CIPTLVDNGF 70 80 90 100 110 120 ALWESRAICT YLAEKYGKDD KLYPKDPQKR AVVNQRMYFD MGTLYQRFAD YYYPQIFAKQ 130 140 150 160 170 180 PANPENEQKM KDAVGFLNSF LDGHKYVAGD SLTIADLSIL ATISTYDVAG FDLAKYQHVA 190 200 AWYENIRKEA PGAAINQAGI EEFKKYFEK
	Isoform A (1-3). See O77462.	FASTA	–	–
	Isoform B (1-4). See O77473.	FASTA	–	–
	Isoform D (1-1) (1-6) (2-1). See Q93113.	FASTA	–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and localization of a glutathione S-transferase class I gene from Anopheles gambiae." Ranson H., Cornel A.J., Fournier D., Vaughan A., Collins F.H., Hemingway J. J. Biol. Chem. 272:5464-5468(1997) [PubMed: 9038148] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Zands. Tissue: Larva.
[2]	"The role of alternative mRNA splicing in generating heterogeneity within the Anopheles gambiae class I glutathione S-transferase family." Ranson H., Collins F.H., Hemingway J. Proc. Natl. Acad. Sci. U.S.A. 95:14284-14289(1998) [PubMed: 9826692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING. Strain: ZAN/U.
[3]	"The genome sequence of the malaria mosquito Anopheles gambiae." Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L. Science 298:129-149(2002) [PubMed: 12364791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PEST.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z81291 mRNA. Translation: CAB03592.1. AF071160 Genomic DNA. Translation: AAC79993.1. AAAB01008880 Genomic DNA. Translation: EAA08605.2.
RefSeq	XP_313049.1.
3D structure databases
HSSP	HSSP built from PDB template 1JLV based on UniProtKB Q9GNE9.
SMR	Q93112. Positions 1-207.
ModBase	Search...
Genome annotation databases
Ensembl	AGAP004164. Anopheles gambiae. [Contig view]
GeneID	1273988.
VectorBase	AGAP004163. Anopheles gambiae.
Phylogenomic databases
OMA	Q93112. KIDESAM.
Enzyme and pathway databases
BRENDA	2.5.1.18. 165157.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00143. Glutathione.

Entry information

Entry name

GST1C_ANOGA

Accession

Primary (citable) accession number: Q93112
Secondary accession number(s): O76482, Q7PQM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 16, 2006
Last modified:	May 5, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)